BuxeA.00134.a

4-hydroxyphenylpyruvate dioxygenase

CENTER ID: BuxeA.00134.a
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00134.a.B1.GE39718 Full length( BuxeA.00134.a ) 1 377
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00134.a.B1.PS37884 Full length( BuxeA.00134.a ) 1 377

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.4917
RefSeq: YP_552572.1
UniProt: Q13RR7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPSDLPTPDA ALRAVSAPEH NPLGTAGLEF VEFAARDPRA LGETFTKLGF KAIARHISKD VTLYRQGEMQ FLINAEPDSF AARYAEEYGA GICAIGIRVA DAQRAFGRAI ELGAWAFEGE KIGAGELLIP AIQGIGDSHI YFVDRWRGRG GQRGGLGDIS IFDIDFRPIQ IDTAQADLSH VGTGLVAVDH LTQTVGEGRM QEWLDFYRDL LNFREIHELH ANWHVSAESR VMVSPCGAIR VPLYEEGTRR TNLMHEYLPD HPGEGVQHIA LATDDIFACV EQLLANGVEF VEPPARYYEQ LDARLPGHGL DVERLRRTHL LVDGEIGTDG VPLLFFQTFV RRRAGEIFFE IVQRQGHHGF GEGNLSALAQ AREQAGA
NT Sequence
ATGCCCAGCG ACTTGCCCAC TCCAGACGCC GCGCTGCGCG CCGTGTCGGC ACCTGAGCAC AATCCGCTCG GCACGGCCGG CCTCGAATTC GTCGAGTTCG CCGCGCGCGA CCCACGCGCG CTCGGCGAAA CCTTCACCAA ACTCGGCTTC AAGGCGATTG CGCGCCATAT CAGCAAGGAC GTCACGCTAT ATCGCCAGGG TGAGATGCAG TTTCTCATCA ACGCCGAGCC CGATTCGTTC GCCGCGCGCT ATGCGGAGGA ATACGGCGCG GGCATCTGCG CGATCGGCAT TCGCGTGGCC GACGCGCAAC GCGCGTTCGG CCGTGCGATC GAACTCGGCG CATGGGCGTT CGAAGGCGAG AAGATCGGCG CCGGCGAGCT GCTGATTCCG GCCATCCAGG GCATTGGCGA CTCGCATATC TATTTCGTCG ACCGCTGGCG CGGCCGCGGC GGCCAGCGTG GCGGCCTCGG CGACATCTCG ATCTTCGACA TCGACTTCCG GCCCATCCAG ATCGACACGG CACAGGCCGA CCTGAGCCAC GTGGGCACCG GCCTCGTCGC AGTCGATCAC CTGACGCAGA CGGTTGGCGA GGGCCGGATG CAGGAATGGC TCGACTTCTA TCGCGACCTG CTCAATTTCC GCGAGATTCA CGAGCTGCAC GCCAACTGGC ATGTATCGGC GGAATCGCGC GTGATGGTCT CGCCATGCGG CGCGATCCGC GTGCCCCTGT ATGAGGAAGG GACGCGCCGG ACCAACCTGA TGCACGAGTA TCTGCCGGAC CATCCGGGCG AAGGCGTGCA GCACATCGCG CTTGCCACCG ACGACATTTT CGCGTGCGTC GAGCAGTTGC TCGCGAACGG CGTGGAATTC GTCGAGCCGC CGGCGCGCTA TTACGAGCAA CTCGACGCAC GTCTGCCGGG CCACGGGCTC GACGTCGAGC GGCTCAGGCG CACGCACCTG CTGGTGGACG GCGAAATCGG CACGGACGGG GTGCCGCTGC TCTTTTTTCA GACCTTCGTG CGCCGCCGCG CCGGCGAAAT ATTCTTCGAG ATCGTGCAGC GCCAGGGACA CCACGGCTTT GGCGAAGGCA ATCTGAGCGC GCTGGCGCAG GCGCGCGAAC AAGCCGGCGC T
Details for BuxeA.00134.a.B1.GE39718
HARVESTED ON: 12/3/2015
SEQUENCED ON: 12/7/2015
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMP SDLPTPDAAL RAVSAPEHNP LGTAGLEFVE FAARDPRALG ETFTKLGFKA IARHISKDVT LYRQGEMQFL INAEPDSFAA RYAEEYGAGI CAIGIRVADA QRAFGRAIEL GAWAFEGEKI GAGELLIPAI QGIGDSHIYF VDRWRGRGGQ RGGLGDISIF DIDFRPIQID TAQADLSHVG TGLVAVDHLT QTVGEGRMQE WLDFYRDLLN FREIHELHAN WHVSAESRVM VSPCGAIRVP LYEEGTRRTN LMHEYLPDHP GEGVQHIALA TDDIFACVEQ LLANGVEFVE PPARYYEQLD ARLPGHGLDV ERLRRTHLLV DGEIGTDGVP LLFFQTFVRR RAGEIFFEIV QRQGHHGFGE GNLS
Validated NT Sequence
cgcgctcaga ttgccttcgc caaagccgtg gtgtccctgg cgctgcacga tctcgaagaa tatttcgccg gcgcggcggc gcacgaaggt ctgaaaaaag agcagcggca ccccgtccgt gccgatttcg ccgtccacca gcaggtgcgt gcgcctgagc cgctcgacgt cgagcccgtg gcccggcaga cgtgcgtcga gttgctcgta atagcgcgcc ggcggctcga cgaattccac gccgttcgcg agcaactgct cgacgcacgc gaaaatgtcg tcggtggcaa gcgcgatgtg ctgcacgcct tcgcccggat ggtccggcag atactcgtgc atcaggttgg tccggcgcgt cccttcctca tacaggggca cgcggatcgc gccgcatggc gagaccatca cgcgcgattc cgccgataca tgccagttgg cgtgcagctc gtgaatctcg cggaaattga gcaggtcgcg atagaagtcg agccattcct gcatccggcc ctcgccaacc gtctgcgtca ggtgatcgac tgcgacgagg ccggtgccca cgtggctcag gtcggcctgt gccgtgtcga tctggatggg ccggaagtcg atgtcgaaga tcgagatgtc gccgaggccg ccacgctggc cgccgcggcc gcgccagcgg tcgacgaaat agatatgcga gtcgccaatg ccctggatgg ccggaatcag cagctcgccg gcgccgatct tctcgccttc gaacgcccat gcgccgagtt cgatcgcacg gccgaacgcg cgttgcgcgt cggccacgcg aatgccgatc gcgcagatgc ccgcgccgta ttcctccgca tagcgcgcgg cgaacgaatc gggctcggcg ttgatgagaa actgcatctc accctggcga tatagcgtga cgtccttgct gatatggcgc gcaatcgcct tgaagccgag tttggtgaag gtttcgccga gcgcgcgtgg gtcgcgcgcg gcgaactcga cgaattcgag gccggccgtg ccgagcggat tgtgctcagg tgccgacacg gcgcgcagcg cggcgtctgg agtgggcaag tcgctgggca tatggtggtg gtggtggtga gccatgg
Expected Protein Sequence
MAHHHHHHMP SDLPTPDAAL RAVSAPEHNP LGTAGLEFVE FAARDPRALG ETFTKLGFKA IARHISKDVT LYRQGEMQFL INAEPDSFAA RYAEEYGAGI CAIGIRVADA QRAFGRAIEL GAWAFEGEKI GAGELLIPAI QGIGDSHIYF VDRWRGRGGQ RGGLGDISIF DIDFRPIQID TAQADLSHVG TGLVAVDHLT QTVGEGRMQE WLDFYRDLLN FREIHELHAN WHVSAESRVM VSPCGAIRVP LYEEGTRRTN LMHEYLPDHP GEGVQHIALA TDDIFACVEQ LLANGVEFVE PPARYYEQLD ARLPGHGLDV ERLRRTHLLV DGEIGTDGVP LLFFQTFVRR RAGEIFFEIV QRQGHHGFGE GNLSALAQAR EQAGA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccca gcgacttgcc cactccagac gccgcgctgc gcgccgtgtc ggcacctgag cacaatccgc tcggcacggc cggcctcgaa ttcgtcgagt tcgccgcgcg cgacccacgc gcgctcggcg aaaccttcac caaactcggc ttcaaggcga ttgcgcgcca tatcagcaag gacgtcacgc tatatcgcca gggtgagatg cagtttctca tcaacgccga gcccgattcg ttcgccgcgc gctatgcgga ggaatacggc gcgggcatct gcgcgatcgg cattcgcgtg gccgacgcgc aacgcgcgtt cggccgtgcg atcgaactcg gcgcatgggc gttcgaaggc gagaagatcg gcgccggcga gctgctgatt ccggccatcc agggcattgg cgactcgcat atctatttcg tcgaccgctg gcgcggccgc ggcggccagc gtggcggcct cggcgacatc tcgatcttcg acatcgactt ccggcccatc cagatcgaca cggcacaggc cgacctgagc cacgtgggca ccggcctcgt cgcagtcgat cacctgacgc agacggttgg cgagggccgg atgcaggaat ggctcgactt ctatcgcgac ctgctcaatt tccgcgagat tcacgagctg cacgccaact ggcatgtatc ggcggaatcg cgcgtgatgg tctcgccatg cggcgcgatc cgcgtgcccc tgtatgagga agggacgcgc cggaccaacc tgatgcacga gtatctgccg gaccatccgg gcgaaggcgt gcagcacatc gcgcttgcca ccgacgacat tttcgcgtgc gtcgagcagt tgctcgcgaa cggcgtggaa ttcgtcgagc cgccggcgcg ctattacgag caactcgacg cacgtctgcc gggccacggg ctcgacgtcg agcggctcag gcgcacgcac ctgctggtgg acggcgaaat cggcacggac ggggtgccgc tgctcttttt tcagaccttc gtgcgccgcc gcgccggcga aatattcttc gagatcgtgc agcgccaggg acaccacggc tttggcgaag gcaatctgag cgcgctggcg caggcgcgcg aacaagccgg cgcttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BuxeA.00134.a.B1.PS37884
PURIFICATION DATe: 3/23/2016
CONCENTRATION: 29.9mg/ml
OBSERVED MW: 42kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMP SDLPTPDAAL RAVSAPEHNP LGTAGLEFVE FAARDPRALG ETFTKLGFKA IARHISKDVT LYRQGEMQFL INAEPDSFAA RYAEEYGAGI CAIGIRVADA QRAFGRAIEL GAWAFEGEKI GAGELLIPAI QGIGDSHIYF VDRWRGRGGQ RGGLGDISIF DIDFRPIQID TAQADLSHVG TGLVAVDHLT QTVGEGRMQE WLDFYRDLLN FREIHELHAN WHVSAESRVM VSPCGAIRVP LYEEGTRRTN LMHEYLPDHP GEGVQHIALA TDDIFACVEQ LLANGVEFVE PPARYYEQLD ARLPGHGLDV ERLRRTHLLV DGEIGTDGVP LLFFQTFVRR RAGEIFFEIV QRQGHHGFGE GNLS
Validated NT Sequence
cgcgctcaga ttgccttcgc caaagccgtg gtgtccctgg cgctgcacga tctcgaagaa tatttcgccg gcgcggcggc gcacgaaggt ctgaaaaaag agcagcggca ccccgtccgt gccgatttcg ccgtccacca gcaggtgcgt gcgcctgagc cgctcgacgt cgagcccgtg gcccggcaga cgtgcgtcga gttgctcgta atagcgcgcc ggcggctcga cgaattccac gccgttcgcg agcaactgct cgacgcacgc gaaaatgtcg tcggtggcaa gcgcgatgtg ctgcacgcct tcgcccggat ggtccggcag atactcgtgc atcaggttgg tccggcgcgt cccttcctca tacaggggca cgcggatcgc gccgcatggc gagaccatca cgcgcgattc cgccgataca tgccagttgg cgtgcagctc gtgaatctcg cggaaattga gcaggtcgcg atagaagtcg agccattcct gcatccggcc ctcgccaacc gtctgcgtca ggtgatcgac tgcgacgagg ccggtgccca cgtggctcag gtcggcctgt gccgtgtcga tctggatggg ccggaagtcg atgtcgaaga tcgagatgtc gccgaggccg ccacgctggc cgccgcggcc gcgccagcgg tcgacgaaat agatatgcga gtcgccaatg ccctggatgg ccggaatcag cagctcgccg gcgccgatct tctcgccttc gaacgcccat gcgccgagtt cgatcgcacg gccgaacgcg cgttgcgcgt cggccacgcg aatgccgatc gcgcagatgc ccgcgccgta ttcctccgca tagcgcgcgg cgaacgaatc gggctcggcg ttgatgagaa actgcatctc accctggcga tatagcgtga cgtccttgct gatatggcgc gcaatcgcct tgaagccgag tttggtgaag gtttcgccga gcgcgcgtgg gtcgcgcgcg gcgaactcga cgaattcgag gccggccgtg ccgagcggat tgtgctcagg tgccgacacg gcgcgcagcg cggcgtctgg agtgggcaag tcgctgggca tatggtggtg gtggtggtga gccatgg
Expressed Protein Sequence
MAHHHHHHMP SDLPTPDAAL RAVSAPEHNP LGTAGLEFVE FAARDPRALG ETFTKLGFKA IARHISKDVT LYRQGEMQFL INAEPDSFAA RYAEEYGAGI CAIGIRVADA QRAFGRAIEL GAWAFEGEKI GAGELLIPAI QGIGDSHIYF VDRWRGRGGQ RGGLGDISIF DIDFRPIQID TAQADLSHVG TGLVAVDHLT QTVGEGRMQE WLDFYRDLLN FREIHELHAN WHVSAESRVM VSPCGAIRVP LYEEGTRRTN LMHEYLPDHP GEGVQHIALA TDDIFACVEQ LLANGVEFVE PPARYYEQLD ARLPGHGLDV ERLRRTHLLV DGEIGTDGVP LLFFQTFVRR RAGEIFFEIV QRQGHHGFGE GNLSALAQAR EQAGA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCCA GCGACTTGCC CACTCCAGAC GCCGCGCTGC GCGCCGTGTC GGCACCTGAG CACAATCCGC TCGGCACGGC CGGCCTCGAA TTCGTCGAGT TCGCCGCGCG CGACCCACGC GCGCTCGGCG AAACCTTCAC CAAACTCGGC TTCAAGGCGA TTGCGCGCCA TATCAGCAAG GACGTCACGC TATATCGCCA GGGTGAGATG CAGTTTCTCA TCAACGCCGA GCCCGATTCG TTCGCCGCGC GCTATGCGGA GGAATACGGC GCGGGCATCT GCGCGATCGG CATTCGCGTG GCCGACGCGC AACGCGCGTT CGGCCGTGCG ATCGAACTCG GCGCATGGGC GTTCGAAGGC GAGAAGATCG GCGCCGGCGA GCTGCTGATT CCGGCCATCC AGGGCATTGG CGACTCGCAT ATCTATTTCG TCGACCGCTG GCGCGGCCGC GGCGGCCAGC GTGGCGGCCT CGGCGACATC TCGATCTTCG ACATCGACTT CCGGCCCATC CAGATCGACA CGGCACAGGC CGACCTGAGC CACGTGGGCA CCGGCCTCGT CGCAGTCGAT CACCTGACGC AGACGGTTGG CGAGGGCCGG ATGCAGGAAT GGCTCGACTT CTATCGCGAC CTGCTCAATT TCCGCGAGAT TCACGAGCTG CACGCCAACT GGCATGTATC GGCGGAATCG CGCGTGATGG TCTCGCCATG CGGCGCGATC CGCGTGCCCC TGTATGAGGA AGGGACGCGC CGGACCAACC TGATGCACGA GTATCTGCCG GACCATCCGG GCGAAGGCGT GCAGCACATC GCGCTTGCCA CCGACGACAT TTTCGCGTGC GTCGAGCAGT TGCTCGCGAA CGGCGTGGAA TTCGTCGAGC CGCCGGCGCG CTATTACGAG CAACTCGACG CACGTCTGCC GGGCCACGGG CTCGACGTCG AGCGGCTCAG GCGCACGCAC CTGCTGGTGG ACGGCGAAAT CGGCACGGAC GGGGTGCCGC TGCTCTTTTT TCAGACCTTC GTGCGCCGCC GCGCCGGCGA AATATTCTTC GAGATCGTGC AGCGCCAGGG ACACCACGGC TTTGGCGAAG GCAATCTGAG CGCGCTGGCG CAGGCGCGCG AACAAGCCGG CGCTTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT