SSGCID: Target Page

BuxeA.00120.a

GTPase obg

CENTER ID:	BuxeA.00120.a
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00120.a.A1.GE33030	Full length( BuxeA.00120.a )		1	373

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00120.a.A1.PS01347	Full length( BuxeA.00120.a )		1	373

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.4106
RefSeq:	YP_560476.1
UniProt:	Q13U15

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKFIDEARIE VIAGDGGDGS ASMRREKFVP FGGPDGGDGG RGGSVIAVAD RNINTLIDYR YAKKHLARNG ENGRGADCYG KGGDDITLRM PVGTTITDME TGELIADLTE HNQSVQIAQG GAGGLGNLHF KSSTNRAPRQ KTDGKPGERR MVRLELKVLA DVGLLGMPNA GKSTFISSVS NARPKIADYP FTTLAPNLGV VRVGPSRSFV IADIPGLIEG AAEGAGLGHQ FLRHLQRTGL LLHIVDLAPF DESVDPVAEA KAIVNELRKY DELLYEKPRW LVLNKLDMVP EDEREARVSA FIEGFGWDGP VFEISALTGQ GCENLCYAVF DYIAAHSDAQ RAAEAEDLAA DVRFRAKPAD PVAADDSGVD PQQ

NT Sequence

ATGAAGTTCA TTGACGAAGC GAGGATTGAA GTCATCGCCG GCGACGGAGG GGATGGCAGC GCGTCGATGC GCCGCGAGAA ATTCGTTCCG TTCGGCGGCC CGGATGGCGG CGACGGCGGC CGGGGCGGCA GCGTGATCGC GGTCGCGGAC CGCAACATCA ACACGCTGAT CGACTATCGC TACGCGAAGA AACACCTGGC GCGCAACGGC GAAAACGGCC GCGGTGCGGA CTGCTACGGC AAGGGCGGCG ACGACATCAC GCTGCGCATG CCGGTGGGCA CCACCATCAC CGACATGGAG ACCGGCGAGC TGATCGCCGA TCTGACCGAG CACAACCAGA GCGTGCAGAT CGCTCAGGGC GGCGCGGGCG GTCTCGGTAA CCTGCATTTC AAATCCAGCA CGAACCGCGC GCCGCGTCAG AAGACGGACG GCAAGCCGGG CGAGCGCCGC ATGGTGCGCC TCGAGTTGAA GGTGTTGGCG GACGTCGGTC TGCTCGGCAT GCCGAACGCG GGCAAGTCGA CCTTCATCTC GTCGGTGTCG AACGCAAGGC CGAAGATCGC CGATTACCCG TTCACCACGC TCGCGCCGAA TCTCGGCGTG GTGCGTGTCG GGCCGAGCCG CAGCTTCGTG ATTGCGGACA TTCCCGGCCT GATCGAGGGC GCGGCGGAAG GCGCGGGCCT CGGTCATCAG TTCCTGCGCC ATCTGCAGCG CACGGGTTTG TTGCTGCACA TCGTCGACCT CGCGCCGTTC GACGAATCGG TCGATCCGGT CGCGGAAGCC AAGGCGATCG TCAACGAACT GCGCAAGTAC GACGAACTGC TGTATGAAAA GCCGCGCTGG CTGGTGTTGA ACAAGCTGGA TATGGTGCCG GAAGACGAGC GCGAAGCGCG TGTCTCGGCA TTTATCGAGG GCTTTGGCTG GGACGGTCCG GTGTTCGAAA TCTCGGCGCT GACTGGCCAG GGCTGTGAAA ACCTTTGCTA CGCGGTGTTC GACTACATCG CCGCGCATTC GGACGCACAG CGCGCGGCCG AGGCCGAAGA TCTCGCCGCC GACGTGCGTT TCCGCGCGAA GCCGGCAGAT CCGGTCGCAG CGGACGACTC CGGCGTCGAC CCGCAGCAA

Details for BuxeA.00120.a.A1.GE33030

HARVESTED ON:	8/16/2011
SEQUENCED ON:	8/22/2011
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMKFIDEA RIEVIAGDGG DGSASMRREK FVPFGGPDGG DGGRGGSVIA VADRNINTLI DYRYAKKHLA RNGENGRGAD CYGKGGDDIT LRMPVGTTIT DMETGELIAD LTEHNQSVQI AQGGAGGLGN LHFKSSTNRA PRQKTDGKPG ERRMVRLELK VLADVGLLGM PNAGKSTFIS SVSNARPKIA DYPFTTLAPN LGVVRVGPSR SFVIADIPGL IEGAAEGAGL GHQFLRHLQR TGLLLHIVDL APFDESVDPV AEAKAIVNEL RKYDELLYEK PRWLVLNKLD MVPEDEREAR VSAFIEGFGW DGPVFEISAL TGQGCENLCY AVFDYIAAHS DAQRAAEAED LAADVRFRAK PADPVAADDS GVD

Validated NT Sequence

cgggtcgacg ccggagtcgt ccgctgcgac cggatctgcc ggcttcgcgc ggaaacgcac gtcggcggcg agatcttcgg cctcggccgc gcgctgtgcg tccgaatgcg cggcgatgta gtcgaacacc gcgtagcaaa ggttttcaca gccctggcca gtcagcgccg agatttcgaa caccggaccg tcccagccaa agccctcgat aaatgccgag acacgcgctt cgcgctcgtc ttccggcacc atatccagct tgttcaacac cagccagcgc ggcttttcat acagcagttc gtcgtacttg cgcagttcgt tgacgatcgc cttggcttcc gcgaccggat cgaccgattc gtcgaacggc gcgaggtcga cgatgtgcag caacaaaccc gtgcgctgca gatggcgcag gaactgatga ccgaggcccg cgccttccgc cgcgccctcg atcaggccgg gaatgtccgc aatcacgaag ctgcggctcg gcccgacacg caccacgccg agattcggcg cgagcgtggt gaacgggtaa tcggcgatct tcggccttgc gttcgacacc gacgagatga aggtcgactt gcccgcgttc ggcatgccga gcagaccgac gtccgccaac accttcaact cgaggcgcac catgcggcgc tcgcccggct tgccgtccgt cttctgacgc ggcgcgcggt tcgtgctgga tttgaaatgc aggttaccga gaccgcccgc gccgccctga gcgatctgca cgctctggtt gtgctcggtc agatcggcga tcagctcgcc ggtctccatg tcggtgatgg tggtgcccac cggcatgcgc agcgtgatgt cgtcgccgcc cttgccgtag cagtccgcac cgcggccgtt ttcgccgttg cgcgccaggt gtttcttcgc gtagcgatag tcgatcagcg tgttgatgtt gcggtccgcg accgcgatca cgctgccgcc ccggccgccg tcgccgccat ccgggccgcc gaacggaacg aatttctcgc ggcgcatcga cgcgctgcca tcccctccgt cgccggcgat gacttcaatc ctcgcttcgt caatgaactt catcgaacca ggaccctggg tctgagcttc cagggtaccc atatg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMKFIDEARI EVIAGDGGDG SASMRREKFV PFGGPDGGDG GRGGSVIAVA DRNINTLIDY RYAKKHLARN GENGRGADCY GKGGDDITLR MPVGTTITDM ETGELIADLT EHNQSVQIAQ GGAGGLGNLH FKSSTNRAPR QKTDGKPGER RMVRLELKVL ADVGLLGMPN AGKSTFISSV SNARPKIADY PFTTLAPNLG VVRVGPSRSF VIADIPGLIE GAAEGAGLGH QFLRHLQRTG LLLHIVDLAP FDESVDPVAE AKAIVNELRK YDELLYEKPR WLVLNKLDMV PEDEREARVS AFIEGFGWDG PVFEISALTG QGCENLCYAV FDYIAAHSDA QRAAEAEDLA ADVRFRAKPA DPVAADDSGV DPQQ

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagttcatt gacgaagcga ggattgaagt catcgccggc gacggagggg atggcagcgc gtcgatgcgc cgcgagaaat tcgttccgtt cggcggcccg gatggcggcg acggcggccg gggcggcagc gtgatcgcgg tcgcggaccg caacatcaac acgctgatcg actatcgcta cgcgaagaaa cacctggcgc gcaacggcga aaacggccgc ggtgcggact gctacggcaa gggcggcgac gacatcacgc tgcgcatgcc ggtgggcacc accatcaccg acatggagac cggcgagctg atcgccgatc tgaccgagca caaccagagc gtgcagatcg ctcagggcgg cgcgggcggt ctcggtaacc tgcatttcaa atccagcacg aaccgcgcgc cgcgtcagaa gacggacggc aagccgggcg agcgccgcat ggtgcgcctc gagttgaagg tgttggcgga cgtcggtctg ctcggcatgc cgaacgcggg caagtcgacc ttcatctcgt cggtgtcgaa cgcaaggccg aagatcgccg attacccgtt caccacgctc gcgccgaatc tcggcgtggt gcgtgtcggg ccgagccgca gcttcgtgat tgcggacatt cccggcctga tcgagggcgc ggcggaaggc gcgggcctcg gtcatcagtt cctgcgccat ctgcagcgca cgggtttgtt gctgcacatc gtcgacctcg cgccgttcga cgaatcggtc gatccggtcg cggaagccaa ggcgatcgtc aacgaactgc gcaagtacga cgaactgctg tatgaaaagc cgcgctggct ggtgttgaac aagctggata tggtgccgga agacgagcgc gaagcgcgtg tctcggcatt tatcgagggc tttggctggg acggtccggt gttcgaaatc tcggcgctga ctggccaggg ctgtgaaaac ctttgctacg cggtgttcga ctacatcgcc gcgcattcgg acgcacagcg cgcggccgag gccgaagatc tcgccgccga cgtgcgtttc cgcgcgaagc cggcagatcc ggtcgcagcg gacgactccg gcgtcgaccc gcagcaataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for BuxeA.00120.a.A1.PS01347

PURIFICATION DATe:	1/20/2012
CONCENTRATION:	103.11mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAGTTCATT GACGAAGCGA GGATTGAAGT CATCGCCGGC GACGGAGGGG ATGGCAGCGC GTCGATGCGC CGCGAGAAAT TCGTTCCGTT CGGCGGCCCG GATGGCGGCG ACGGCGGCCG GGGCGGCAGC GTGATCGCGG TCGCGGACCG CAACATCAAC ACGCTGATCG ACTATCGCTA CGCGAAGAAA CACCTGGCGC GCAACGGCGA AAACGGCCGC GGTGCGGACT GCTACGGCAA GGGCGGCGAC GACATCACGC TGCGCATGCC GGTGGGCACC ACCATCACCG ACATGGAGAC CGGCGAGCTG ATCGCCGATC TGACCGAGCA CAACCAGAGC GTGCAGATCG CTCAGGGCGG CGCGGGCGGT CTCGGTAACC TGCATTTCAA ATCCAGCACG AACCGCGCGC CGCGTCAGAA GACGGACGGC AAGCCGGGCG AGCGCCGCAT GGTGCGCCTC GAGTTGAAGG TGTTGGCGGA CGTCGGTCTG CTCGGCATGC CGAACGCGGG CAAGTCGACC TTCATCTCGT CGGTGTCGAA CGCAAGGCCG AAGATCGCCG ATTACCCGTT CACCACGCTC GCGCCGAATC TCGGCGTGGT GCGTGTCGGG CCGAGCCGCA GCTTCGTGAT TGCGGACATT CCCGGCCTGA TCGAGGGCGC GGCGGAAGGC GCGGGCCTCG GTCATCAGTT CCTGCGCCAT CTGCAGCGCA CGGGTTTGTT GCTGCACATC GTCGACCTCG CGCCGTTCGA CGAATCGGTC GATCCGGTCG CGGAAGCCAA GGCGATCGTC AACGAACTGC GCAAGTACGA CGAACTGCTG TATGAAAAGC CGCGCTGGCT GGTGTTGAAC AAGCTGGATA TGGTGCCGGA AGACGAGCGC GAAGCGCGTG TCTCGGCATT TATCGAGGGC TTTGGCTGGG ACGGTCCGGT GTTCGAAATC TCGGCGCTGA CTGGCCAGGG CTGTGAAAAC CTTTGCTACG CGGTGTTCGA CTACATCGCC GCGCATTCGG ACGCACAGCG CGCGGCCGAG GCCGAAGATC TCGCCGCCGA CGTGCGTTTC CGCGCGAAGC CGGCAGATCC GGTCGCAGCG GACGACTCCG GCGTCGACCC GCAGCAATAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA