BuxeA.00119.a
D-alanine--D-alanine ligase
| CENTER ID: | BuxeA.00119.a |
| ORGANISM: | Burkholderia xenovorans LB400 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuxeA.00119.a.A1.GE33029 | Full length( BuxeA.00119.a ) | 1 | 313 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuxeA.00119.a.A1.PS01358 | Full length( BuxeA.00119.a ) | 1 | 313 |
Structures
4EGJ
DEPOSITED: 3/31/2012
DETERMINATION: XRay
CLONE: BuxeA.00119.a.A1.GE33029
PROTEIN: BuxeA.00119.a.A1.PS01358
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_130117 |
| PATRIC ID: | fig|266265.5.peg.4128 |
| RefSeq: | YP_560497.1 |
| UniProt: | Q13TZ4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSIDPKQFG KVAVLLGGNS AEREVSLNSG RLVLQGLRDA GIDAHPFDPA ERPLAALKEE GFVRAFNALH GGYGENGQIQ GALDFYGIRY TGSGVLGSAL GLDKFRTKLV WQQLGIPTPP FEAVLRGDDY EARAKEIVAK LGLPLFVKPA SEGSSVAVIK VKSADALPAA LIEAVKFDRI VVVEKSIEGG GEYTACIAGN LDLPVIRIVP AGEFYDYHAK YIANDTQYLI PCGLTADEEA RLKVLARRAF DVLGCTDWGR ADFMLDADGN PYFLEVNTAP GMTDHSLPPK AARAVGISYQ ELVVAVLALT LKD
NT Sequence
ATGAGCAGTA TCGACCCCAA ACAGTTCGGC AAGGTGGCAG TGCTGCTCGG CGGTAATTCC GCCGAGCGCG AGGTGTCGCT CAATTCCGGG CGTCTCGTGC TTCAAGGTCT GCGCGACGCC GGCATCGACG CGCATCCGTT CGATCCGGCC GAGCGTCCGC TCGCCGCATT GAAGGAAGAG GGCTTCGTGC GCGCGTTCAA CGCGCTGCAC GGCGGCTACG GCGAGAACGG CCAGATCCAG GGCGCGCTCG ATTTTTACGG CATCCGCTAC ACGGGCAGCG GCGTGCTCGG CTCGGCGCTC GGTCTGGACA AGTTCCGCAC CAAGCTCGTG TGGCAGCAAC TCGGTATTCC GACGCCGCCG TTCGAAGCCG TGCTGCGCGG CGACGACTAC GAAGCGCGCG CCAAGGAGAT CGTCGCGAAG CTCGGTTTGC CGCTTTTCGT GAAGCCGGCG AGCGAAGGTT CGAGCGTCGC GGTAATCAAG GTGAAAAGCG CAGACGCGCT GCCCGCCGCG TTGATCGAAG CAGTGAAGTT CGACAGGATC GTGGTGGTCG AAAAGAGTAT TGAAGGCGGC GGCGAATACA CCGCGTGCAT CGCCGGCAAT CTGGATTTGC CGGTGATCCG CATCGTGCCG GCCGGCGAGT TTTACGATTA CCACGCGAAG TACATCGCGA ACGACACGCA GTATCTGATT CCGTGCGGTC TGACGGCGGA CGAGGAGGCG CGTCTGAAAG TGCTGGCGCG CCGTGCCTTC GACGTGCTCG GCTGCACCGA CTGGGGCCGC GCGGATTTCA TGCTCGACGC CGACGGCAAC CCGTATTTCC TCGAAGTGAA CACGGCGCCC GGCATGACCG ATCACTCGCT GCCGCCGAAA GCGGCGAGAG CGGTGGGCAT CAGTTATCAG GAACTCGTTG TCGCCGTGTT GGCGCTGACG CTCAAGGAC
Details for BuxeA.00119.a.A1.GE33029
| HARVESTED ON: | 8/16/2011 |
| SEQUENCED ON: | 8/22/2011 |
| EXPECTED MW: | 35kDa |
| OBSERVED MW: | 35kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MGTLEAQTQG PGSMSSIDPK QFGKVAVLLG GNSAEREVSL NSGRLVLQGL RDAGIDAHPF DPAERPLAAL KEEGFVRAFN ALHGGYGENG QIQGALDFYG IRYTGSGVLG SALGLDKFRT KLVWQQLGIP TPPFEAVLRG DDYEARAKEI VAKLGLPLFV KPASEGSSVA VIKVKSADAL PAALIEAVKF DRIVVVEKSI EGGGEYTACI AGNLDLPVIR IVPAGEFYDY HAKYIANDTQ YLIPCGLTAD EEARLKVLAR RAFDVLGCTD WGRADFMLDA DGNPYFLEVN TAPGMTDHSL PPKAARAVGI SYQELVVAVL ALTL
Validated NT Sequence
tgagcgtcag cgccaacacg gcgacaacga gttcctgata actgatgccc accgctctcg ccgctttcgg cggcagcgag tgatcggtca tgccgggcgc cgtgttcact tcgaggaaat acgggttgcc gtcggcgtcg agcatgaaat ccgcgcggcc ccagtcggtg cagccgagca cgtcgaaggc acggcgcgcc agcactttca gacgcgcctc ctcgtccgcc gtcagaccgc acggaatcag atactgcgtg tcgttcgcga tgtacttcgc gtggtaatcg taaaactcgc cggccggcac gatgcggatc accggcaaat ccagattgcc ggcgatgcac gcggtgtatt cgccgccgcc ttcaatactc ttttcgacca ccacgatcct gtcgaacttc actgcttcga tcaacgcggc gggcagcgcg tctgcgcttt tcaccttgat taccgcgacg ctcgaacctt cgctcgccgg cttcacgaaa agcggcaaac cgagcttcgc gacgatctcc ttggcgcgcg cttcgtagtc gtcgccgcgc agcacggctt cgaacggcgg cgtcggaata ccgagttgct gccacacgag cttggtgcgg aacttgtcca gaccgagcgc cgagccgagc acgccgctgc ccgtgtagcg gatgccgtaa aaatcgagcg cgccctggat ctggccgttc tcgccgtagc cgccgtgcag cgcgttgaac gcgcgcacga agccctcttc cttcaatgcg gcgagcggac gctcggccgg atcgaacgga tgcgcgtcga tgccggcgtc gcgcagacct tgaagcacga gacgcccgga attgagcgac acctcgcgct cggcggaatt accgccgagc agcactgcca ccttgccgaa ctgtttgggg tcgatactgc tcatcgaacc aggaccctgg gtctgagctt ccagggtacc catatga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSIDPKQF GKVAVLLGGN SAEREVSLNS GRLVLQGLRD AGIDAHPFDP AERPLAALKE EGFVRAFNAL HGGYGENGQI QGALDFYGIR YTGSGVLGSA LGLDKFRTKL VWQQLGIPTP PFEAVLRGDD YEARAKEIVA KLGLPLFVKP ASEGSSVAVI KVKSADALPA ALIEAVKFDR IVVVEKSIEG GGEYTACIAG NLDLPVIRIV PAGEFYDYHA KYIANDTQYL IPCGLTADEE ARLKVLARRA FDVLGCTDWG RADFMLDADG NPYFLEVNTA PGMTDHSLPP KAARAVGISY QELVVAVLAL TLKD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcagtatc gaccccaaac agttcggcaa ggtggcagtg ctgctcggcg gtaattccgc cgagcgcgag gtgtcgctca attccgggcg tctcgtgctt caaggtctgc gcgacgccgg catcgacgcg catccgttcg atccggccga gcgtccgctc gccgcattga aggaagaggg cttcgtgcgc gcgttcaacg cgctgcacgg cggctacggc gagaacggcc agatccaggg cgcgctcgat ttttacggca tccgctacac gggcagcggc gtgctcggct cggcgctcgg tctggacaag ttccgcacca agctcgtgtg gcagcaactc ggtattccga cgccgccgtt cgaagccgtg ctgcgcggcg acgactacga agcgcgcgcc aaggagatcg tcgcgaagct cggtttgccg cttttcgtga agccggcgag cgaaggttcg agcgtcgcgg taatcaaggt gaaaagcgca gacgcgctgc ccgccgcgtt gatcgaagca gtgaagttcg acaggatcgt ggtggtcgaa aagagtattg aaggcggcgg cgaatacacc gcgtgcatcg ccggcaatct ggatttgccg gtgatccgca tcgtgccggc cggcgagttt tacgattacc acgcgaagta catcgcgaac gacacgcagt atctgattcc gtgcggtctg acggcggacg aggaggcgcg tctgaaagtg ctggcgcgcc gtgccttcga cgtgctcggc tgcaccgact ggggccgcgc ggatttcatg ctcgacgccg acggcaaccc gtatttcctc gaagtgaaca cggcgcccgg catgaccgat cactcgctgc cgccgaaagc ggcgagagcg gtgggcatca gttatcagga actcgttgtc gccgtgttgg cgctgacgct caaggactaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BuxeA.00119.a.A1.PS01358
| PURIFICATION DATe: | 1/24/2012 |
| CONCENTRATION: | 115.9mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMSSIDPK QFGKVAVLLG GNSAEREVSL NSGRLVLQGL RDAGIDAHPF DPAERPLAAL KEEGFVRAFN ALHGGYGENG QIQGALDFYG IRYTGSGVLG SALGLDKFRT KLVWQQLGIP TPPFEAVLRG DDYEARAKEI VAKLGLPLFV KPASEGSSVA VIKVKSADAL PAALIEAVKF DRIVVVEKSI EGGGEYTACI AGNLDLPVIR IVPAGEFYDY HAKYIANDTQ YLIPCGLTAD EEARLKVLAR RAFDVLGCTD WGRADFMLDA DGNPYFLEVN TAPGMTDHSL PPKAARAVGI SYQELVVAVL ALTL
Validated NT Sequence
tgagcgtcag cgccaacacg gcgacaacga gttcctgata actgatgccc accgctctcg ccgctttcgg cggcagcgag tgatcggtca tgccgggcgc cgtgttcact tcgaggaaat acgggttgcc gtcggcgtcg agcatgaaat ccgcgcggcc ccagtcggtg cagccgagca cgtcgaaggc acggcgcgcc agcactttca gacgcgcctc ctcgtccgcc gtcagaccgc acggaatcag atactgcgtg tcgttcgcga tgtacttcgc gtggtaatcg taaaactcgc cggccggcac gatgcggatc accggcaaat ccagattgcc ggcgatgcac gcggtgtatt cgccgccgcc ttcaatactc ttttcgacca ccacgatcct gtcgaacttc actgcttcga tcaacgcggc gggcagcgcg tctgcgcttt tcaccttgat taccgcgacg ctcgaacctt cgctcgccgg cttcacgaaa agcggcaaac cgagcttcgc gacgatctcc ttggcgcgcg cttcgtagtc gtcgccgcgc agcacggctt cgaacggcgg cgtcggaata ccgagttgct gccacacgag cttggtgcgg aacttgtcca gaccgagcgc cgagccgagc acgccgctgc ccgtgtagcg gatgccgtaa aaatcgagcg cgccctggat ctggccgttc tcgccgtagc cgccgtgcag cgcgttgaac gcgcgcacga agccctcttc cttcaatgcg gcgagcggac gctcggccgg atcgaacgga tgcgcgtcga tgccggcgtc gcgcagacct tgaagcacga gacgcccgga attgagcgac acctcgcgct cggcggaatt accgccgagc agcactgcca ccttgccgaa ctgtttgggg tcgatactgc tcatcgaacc aggaccctgg gtctgagctt ccagggtacc catatga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSIDPKQF GKVAVLLGGN SAEREVSLNS GRLVLQGLRD AGIDAHPFDP AERPLAALKE EGFVRAFNAL HGGYGENGQI QGALDFYGIR YTGSGVLGSA LGLDKFRTKL VWQQLGIPTP PFEAVLRGDD YEARAKEIVA KLGLPLFVKP ASEGSSVAVI KVKSADALPA ALIEAVKFDR IVVVEKSIEG GGEYTACIAG NLDLPVIRIV PAGEFYDYHA KYIANDTQYL IPCGLTADEE ARLKVLARRA FDVLGCTDWG RADFMLDADG NPYFLEVNTA PGMTDHSLPP KAARAVGISY QELVVAVLAL TLKD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCAGTATC GACCCCAAAC AGTTCGGCAA GGTGGCAGTG CTGCTCGGCG GTAATTCCGC CGAGCGCGAG GTGTCGCTCA ATTCCGGGCG TCTCGTGCTT CAAGGTCTGC GCGACGCCGG CATCGACGCG CATCCGTTCG ATCCGGCCGA GCGTCCGCTC GCCGCATTGA AGGAAGAGGG CTTCGTGCGC GCGTTCAACG CGCTGCACGG CGGCTACGGC GAGAACGGCC AGATCCAGGG CGCGCTCGAT TTTTACGGCA TCCGCTACAC GGGCAGCGGC GTGCTCGGCT CGGCGCTCGG TCTGGACAAG TTCCGCACCA AGCTCGTGTG GCAGCAACTC GGTATTCCGA CGCCGCCGTT CGAAGCCGTG CTGCGCGGCG ACGACTACGA AGCGCGCGCC AAGGAGATCG TCGCGAAGCT CGGTTTGCCG CTTTTCGTGA AGCCGGCGAG CGAAGGTTCG AGCGTCGCGG TAATCAAGGT GAAAAGCGCA GACGCGCTGC CCGCCGCGTT GATCGAAGCA GTGAAGTTCG ACAGGATCGT GGTGGTCGAA AAGAGTATTG AAGGCGGCGG CGAATACACC GCGTGCATCG CCGGCAATCT GGATTTGCCG GTGATCCGCA TCGTGCCGGC CGGCGAGTTT TACGATTACC ACGCGAAGTA CATCGCGAAC GACACGCAGT ATCTGATTCC GTGCGGTCTG ACGGCGGACG AGGAGGCGCG TCTGAAAGTG CTGGCGCGCC GTGCCTTCGA CGTGCTCGGC TGCACCGACT GGGGCCGCGC GGATTTCATG CTCGACGCCG ACGGCAACCC GTATTTCCTC GAAGTGAACA CGGCGCCCGG CATGACCGAT CACTCGCTGC CGCCGAAAGC GGCGAGAGCG GTGGGCATCA GTTATCAGGA ACTCGTTGTC GCCGTGTTGG CGCTGACGCT CAAGGACTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA