SSGCID: Target Page

BuxeA.00118.b

UDP-glucose pyrophosphorylase

CENTER ID:	BuxeA.00118.b
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00118.b.B1.GE39510	Full length( BuxeA.00118.b )		1	293

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00118.b.B1.PS37951	Full length( BuxeA.00118.b )		1	293

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.6008
RefSeq:	YP_553579.1
UniProt:	Q13NW0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLKVTKAVFP VAGLGTRFLP ATKASPKEML PIVDKPLIQY AVEEAMAAGI TEMIFVTGRS KRAIEDHFDK SYEVEAELEA RGKDKLLQLV RSIKPSHVDC FYVRQPEALG LGHAVLCAEK LVGDSPFAVI LADDLLYGSP PVMTQMIEVF DHYHSSVIGV EEIPAQDTKS YGIVDGKEWE QSIIKMSGIV EKPEPSAAPS NLGVVGRYVL KPRIFKHLRT IKPGAGGELQ LTDAIESLLA DEQVLAYKYR GTRFDCGSKI GYLKATVEFA VRHPEVGVEF REYLEEYLSL LPM

NT Sequence

ATGCTAAAAG TTACAAAGGC AGTCTTTCCG GTAGCAGGCC TTGGCACGCG ATTTCTCCCT GCCACGAAGG CGAGCCCAAA GGAGATGCTG CCGATCGTGG ATAAGCCGCT CATCCAATAT GCGGTCGAAG AGGCTATGGC CGCCGGCATC ACTGAAATGA TTTTCGTGAC GGGCCGCAGC AAGCGCGCGA TCGAAGACCA TTTCGACAAG TCGTATGAGG TCGAGGCGGA ACTGGAAGCG CGCGGCAAGG ACAAGCTGCT GCAATTGGTA CGCAGCATCA AGCCGAGCCA TGTCGACTGT TTTTACGTGC GCCAACCGGA GGCGCTCGGC CTCGGGCACG CGGTGCTATG CGCGGAGAAG CTGGTAGGCG ACAGTCCGTT CGCCGTGATT CTCGCGGACG ACCTGCTATA CGGCAGCCCT CCTGTCATGA CGCAAATGAT CGAGGTGTTC GATCACTACC ATAGCTCGGT GATCGGCGTT GAGGAAATTC CGGCGCAGGA TACCAAATCG TACGGCATCG TCGACGGCAA GGAATGGGAG CAGTCCATCA TCAAGATGTC GGGCATCGTG GAAAAGCCCG AGCCGAGCGC GGCTCCTTCA AACCTCGGTG TTGTGGGTCG ATATGTGCTG AAGCCGCGCA TTTTCAAACA TCTGCGCACC ATCAAGCCCG GTGCCGGTGG CGAACTCCAG TTGACGGATG CGATCGAGTC GCTGCTTGCC GATGAGCAGG TACTGGCCTA CAAGTACCGC GGCACGCGCT TTGATTGCGG CAGCAAAATC GGCTACCTGA AGGCCACGGT GGAATTCGCG GTGCGGCATC CCGAGGTAGG CGTCGAGTTT CGTGAGTACC TTGAGGAGTA TCTGTCTCTG CTGCCGATG

Details for BuxeA.00118.b.B1.GE39510

HARVESTED ON:	9/10/2015
SEQUENCED ON:	9/16/2015
EXPECTED MW:	33kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML KVTKAVFPVA GLGTRFLPAT KASPKEMLPI VDKPLIQYAV EEAMAAGITE MIFVTGRSKR AIEDHFDKSY EVEAELEARG KDKLLQLVRS IKPSHVDCFY VRQPEALGLG HAVLCAEKLV GDSPFAVILA DDLLYGSPPV MTQMIEVFDH YHSSVIGVEE IPAQDTKSYG IVDGKEWEQS IIKMSGIVEK PEPSAAPSNL GVVGRYVLKP RIFKHLRTIK PGAGGELQLT DAIESLLADE QVLAYKYRGT RFDCGSKIGY LKATVEFAVR HPEVGVEFRE YLEXXXSLLP M

Validated NT Sequence

tcttactcac ttacatcggc agcagagaca nnnnnncctc aaggtactca cgaaactcga cgcctacctc gggatgccgc accgcgaatt ccaccgtggc cttcaggtag ccgattttgc tgccgcaatc aaagcgcgtg ccgcggtact tgtaggccag tacctgctca tcggcaagca gcgactcgat cgcatccgtc aactggagtt cgccaccggc accgggcttg atggtgcgca gatgtttgaa aatgcgcggc ttcagcacat atcgacccac aacaccgagg tttgaaggag ccgcgctcgg ctcgggcttt tccacgatgc ccgacatctt gatgatggac tgctcccatt ccttgccgtc gacgatgccg tacgatttgg tatcctgcgc cggaatttcc tcaacgccga tcaccgagct atggtagtga tcgaacacct cgatcatttg cgtcatgaca ggagggctgc cgtatagcag gtcgtccgcg agaatcacgg cgaacggact gtcgcctacc agcttctccg cgcatagcac cgcgtgcccg aggccgagcg cctccggttg gcgcacgtaa aaacagtcga catggctcgg cttgatgctg cgtaccaatt gcagcagctt gtccttgccg cgcgcttcca gttccgcctc gacctcatac gacttgtcga aatggtcttc gatcgcgcgc ttgctgcggc ccgtcacgaa aatcatttca gtgatgccgg cggccatagc ctcttcgacc gcatattgga tgagcggctt atccacgatc ggcagcatct cctttgggct cgccttcgtg gcagggagaa atcgcgtgcc aaggcctgct accggaaaga ctgcctttgt aacttttagc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctaa aagttacaaa ggcagtcttt ccggtagcag gccttggcac gcgatttctc cctgccacga aggcgagccc aaaggagatg ctgccgatcg tggataagcc gctcatccaa tatgcggtcg aagaggctat ggccgccggc atcactgaaa tgattttcgt gacgggccgc agcaagcgcg cgatcgaaga ccatttcgac aagtcgtatg aggtcgaggc ggaactggaa gcgcgcggca aggacaagct gctgcaattg gtacgcagca tcaagccgag ccatgtcgac tgtttttacg tgcgccaacc ggaggcgctc ggcctcgggc acgcggtgct atgcgcggag aagctggtag gcgacagtcc gttcgccgtg attctcgcgg acgacctgct atacggcagc cctcctgtca tgacgcaaat gatcgaggtg ttcgatcact accatagctc ggtgatcggc gttgaggaaa ttccggcgca ggataccaaa tcgtacggca tcgtcgacgg caaggaatgg gagcagtcca tcatcaagat gtcgggcatc gtggaaaagc ccgagccgag cgcggctcct tcaaacctcg gtgttgtggg tcgatatgtg ctgaagccgc gcattttcaa acatctgcgc accatcaagc ccggtgccgg tggcgaactc cagttgacgg atgcgatcga gtcgctgctt gccgatgagc aggtactggc ctacaagtac cgcggcacgc gctttgattg cggcagcaaa atcggctacc tgaaggccac ggtggaattc gcggtgcggc atcccgaggt aggcgtcgag tttcgtgagt accttgagga gtatctgtct ctgctgccga tgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for BuxeA.00118.b.B1.PS37951

PURIFICATION DATe:	6/23/2016
CONCENTRATION:	8.8mg/ml
OBSERVED MW:	35kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	SEC Buffer
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	24
VIAL VOLUME:	27µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCTAA AAGTTACAAA GGCAGTCTTT CCGGTAGCAG GCCTTGGCAC GCGATTTCTC CCTGCCACGA AGGCGAGCCC AAAGGAGATG CTGCCGATCG TGGATAAGCC GCTCATCCAA TATGCGGTCG AAGAGGCTAT GGCCGCCGGC ATCACTGAAA TGATTTTCGT GACGGGCCGC AGCAAGCGCG CGATCGAAGA CCATTTCGAC AAGTCGTATG AGGTCGAGGC GGAACTGGAA GCGCGCGGCA AGGACAAGCT GCTGCAATTG GTACGCAGCA TCAAGCCGAG CCATGTCGAC TGTTTTTACG TGCGCCAACC GGAGGCGCTC GGCCTCGGGC ACGCGGTGCT ATGCGCGGAG AAGCTGGTAG GCGACAGTCC GTTCGCCGTG ATTCTCGCGG ACGACCTGCT ATACGGCAGC CCTCCTGTCA TGACGCAAAT GATCGAGGTG TTCGATCACT ACCATAGCTC GGTGATCGGC GTTGAGGAAA TTCCGGCGCA GGATACCAAA TCGTACGGCA TCGTCGACGG CAAGGAATGG GAGCAGTCCA TCATCAAGAT GTCGGGCATC GTGGAAAAGC CCGAGCCGAG CGCGGCTCCT TCAAACCTCG GTGTTGTGGG TCGATATGTG CTGAAGCCGC GCATTTTCAA ACATCTGCGC ACCATCAAGC CCGGTGCCGG TGGCGAACTC CAGTTGACGG ATGCGATCGA GTCGCTGCTT GCCGATGAGC AGGTACTGGC CTACAAGTAC CGCGGCACGC GCTTTGATTG CGGCAGCAAA ATCGGCTACC TGAAGGCCAC GGTGGAATTC GCGGTGCGGC ATCCCGAGGT AGGCGTCGAG TTTCGTGAGT ACCTTGAGGA GTATCTGTCT CTGCTGCCGA TGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT