BuxeA.00116.b

4-hydroxythreonine-4-phosphate dehydrogenase

CENTER ID: BuxeA.00116.b
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00116.b.A1.GE33028 Full length( BuxeA.00116.b ) 1 336
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.463
RefSeq: YP_557029.1
UniProt: Q145L2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTAAQSVRP PLQIAITTGE PAGVGPELTA QALAGAAAHW PHAQFIVLGD ADLLAERARA VGVDWRALVA DGKRVRLQHR PLAAPAVAGK LNAANGRYVL DLLDSAIDAA VAGTFDAIVT APLQKSTIND AGVPFTGHTE YLAERTHTPR VVMMLAGTGK RPLRVALATT HLPLKDVSAA LSIEGILETL RIIDHDLRHH FGLPAPRILV TGLNPHAGEN GYLGREEIEV ITPALKLADA QGIDARGPYP ADTLFQPRYL EQADCVLAMF HDQGLPVLKY ATFGEGINVT LGLPIIRTSV DHGTALDLAG TGRADAGSLI AAIDTAVSMA QHRRAG
NT Sequence
ATGACAACCG CCGCCCAGTC CGTCCGCCCA CCGTTGCAGA TCGCGATCAC GACCGGCGAG CCCGCCGGCG TCGGCCCCGA GCTGACGGCG CAGGCGCTCG CCGGCGCGGC GGCGCATTGG CCCCACGCGC AGTTCATCGT GCTGGGCGAC GCGGACCTGC TGGCCGAACG CGCGCGCGCC GTGGGTGTCG ATTGGCGCGC GCTGGTGGCG GACGGCAAGC GCGTGCGCCT GCAACACCGG CCGCTTGCGG CGCCTGCGGT CGCGGGCAAG CTGAACGCGG CCAACGGCCG CTACGTGCTC GATCTGCTCG ACAGCGCAAT CGACGCAGCG GTGGCCGGCA CCTTCGACGC GATCGTGACC GCGCCGCTGC AAAAGAGCAC CATCAACGAC GCCGGCGTGC CGTTCACCGG CCACACCGAA TATCTGGCCG AGCGCACGCA TACGCCGCGC GTGGTGATGA TGCTGGCCGG CACCGGCAAG CGGCCGTTGC GGGTCGCGCT GGCAACCACG CATCTGCCGC TCAAGGATGT TTCCGCCGCG CTGTCGATCG AAGGCATTCT CGAAACGCTT CGCATCATCG ATCACGATTT GCGGCATCAC TTCGGCTTGC CGGCGCCGCG TATTCTCGTG ACCGGGCTGA ACCCGCACGC GGGCGAAAAC GGTTATCTTG GCCGCGAGGA AATCGAGGTC ATCACGCCGG CGCTAAAGCT CGCGGACGCT CAAGGCATCG ACGCGCGCGG CCCGTACCCG GCCGACACGC TGTTCCAGCC GCGTTACCTG GAGCAGGCCG ACTGCGTGCT GGCCATGTTC CACGACCAGG GTCTGCCGGT GTTGAAGTAC GCCACGTTCG GCGAAGGCAT CAATGTCACG CTCGGCTTGC CGATCATCCG CACTTCGGTC GATCACGGCA CCGCGCTCGA CCTGGCGGGC ACGGGCCGCG CCGACGCGGG CAGCCTGATC GCTGCGATCG ATACGGCGGT GTCGATGGCG CAGCATCGCC GCGCGGGC
Details for BuxeA.00116.b.A1.GE33028
HARVESTED ON: 8/16/2011
SEQUENCED ON: 8/22/2011
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 86
Validated AA Sequence
MTTAAQSVRP PLQIAITTGE PAGVGPELTA QALAGAAAHW PHAQFIVLGD ADLLAERARA VGVDWRALVA DGKRVRLQHR PLAAPAVAGK LNAANGRYVL DLLDSAIDAA VAGTFDAIVT APLQKSTIND AGVPFTGHTE YLAERTHTPR VVMMLAGTGK RPLRVALATT HLPLKDVSAA LSIEGILETL RIIDHDLRHH FGLPAPRILV TGLNPHAGEN GYLGREEIEV ITPALKLADA QGIDARGPYP ADTLFQPRYL EQADCVLAMF HDQGLPVLKY ATFGEGINVT LGLPIIRTSV DHGTALDL
Validated NT Sequence
ccaggtcgag cgcggtgccg tgatcgaccg aagtgcggat gatcggcaag ccgagcgtga cattgatgcc ttcgccgaac gtggcgtact tcaacaccgg cagaccctgg tcgtggaaca tggccagcac gcagtcggcc tgctccaggt aacgcggctg gaacagcgtg tcggccgggt acgggccgcg cgcgtcgatg ccttgagcgt ccgcgagctt tagcgccggc gtgatgacct cgatttcctc gcggccaaga taaccgtttt cgcccgcgtg cgggttcagc ccggtcacga gaatacgcgg cgccggcaag ccgaagtgat gccgcaaatc gtgatcgatg atgcgaagcg tttcgagaat gccttcgatc gacagcgcgg cggaaacatc cttgagcggc agatgcgtgg ttgccagcgc gacccgcaac ggccgcttgc cggtgccggc cagcatcatc accacgcgcg gcgtatgcgt gcgctcggcc agatattcgg tgtggccggt gaacggcacg ccggcgtcgt tgatggtgct cttttgcagc ggcgcggtca cgatcgcgtc gaaggtgccg gccaccgctg cgtcgattgc gctgtcgagc agatcgagca cgtagcggcc gttggccgcg ttcagcttgc ccgcgaccgc aggcgccgca agcggccggt gttgcaggcg cacgcgcttg ccgtccgcca ccagcgcgcg ccaatcgaca cccacggcgc gcgcgcgttc ggccagcagg tccgcgtcgc ccagcacgat gaactgcgcg tggggccaat gcgccgccgc gccggcgagc gcctgcgccg tcagctcggg gccgacgccg gcgggctcgc cggtcgtgat cgcgatctgc aacggtgggc ggacggactg ggcggcggtt gtcatcgaac caggaccctg ggtctgagct tccaggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTAAQSVR PPLQIAITTG EPAGVGPELT AQALAGAAAH WPHAQFIVLG DADLLAERAR AVGVDWRALV ADGKRVRLQH RPLAAPAVAG KLNAANGRYV LDLLDSAIDA AVAGTFDAIV TAPLQKSTIN DAGVPFTGHT EYLAERTHTP RVVMMLAGTG KRPLRVALAT THLPLKDVSA ALSIEGILET LRIIDHDLRH HFGLPAPRIL VTGLNPHAGE NGYLGREEIE VITPALKLAD AQGIDARGPY PADTLFQPRY LEQADCVLAM FHDQGLPVLK YATFGEGINV TLGLPIIRTS VDHGTALDLA GTGRADAGSL IAAIDTAVSM AQHRRAG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaaccgcc gcccagtccg tccgcccacc gttgcagatc gcgatcacga ccggcgagcc cgccggcgtc ggccccgagc tgacggcgca ggcgctcgcc ggcgcggcgg cgcattggcc ccacgcgcag ttcatcgtgc tgggcgacgc ggacctgctg gccgaacgcg cgcgcgccgt gggtgtcgat tggcgcgcgc tggtggcgga cggcaagcgc gtgcgcctgc aacaccggcc gcttgcggcg cctgcggtcg cgggcaagct gaacgcggcc aacggccgct acgtgctcga tctgctcgac agcgcaatcg acgcagcggt ggccggcacc ttcgacgcga tcgtgaccgc gccgctgcaa aagagcacca tcaacgacgc cggcgtgccg ttcaccggcc acaccgaata tctggccgag cgcacgcata cgccgcgcgt ggtgatgatg ctggccggca ccggcaagcg gccgttgcgg gtcgcgctgg caaccacgca tctgccgctc aaggatgttt ccgccgcgct gtcgatcgaa ggcattctcg aaacgcttcg catcatcgat cacgatttgc ggcatcactt cggcttgccg gcgccgcgta ttctcgtgac cgggctgaac ccgcacgcgg gcgaaaacgg ttatcttggc cgcgaggaaa tcgaggtcat cacgccggcg ctaaagctcg cggacgctca aggcatcgac gcgcgcggcc cgtacccggc cgacacgctg ttccagccgc gttacctgga gcaggccgac tgcgtgctgg ccatgttcca cgaccagggt ctgccggtgt tgaagtacgc cacgttcggc gaaggcatca atgtcacgct cggcttgccg atcatccgca cttcggtcga tcacggcacc gcgctcgacc tggcgggcac gggccgcgcc gacgcgggca gcctgatcgc tgcgatcgat acggcggtgt cgatggcgca gcatcgccgc gcgggctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa