BuxeA.00116.a

Putative terephthalate dihydrodiol dehydrogenase(TphB)

CENTER ID: BuxeA.00116.a
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00116.a.A1.GE33027 Full length( BuxeA.00116.a ) 1 349
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.4944
RefSeq: YP_552595.1
UniProt: Q13RP4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRTRDRAMT VALAIGDPNG IGPEIAVKAA AQMARDERSE HGGHAVHSQP RIVLFGDAFV IRRYARQCCP ELMLRLVQLD DLPSAEPNAI DMVDVASLPA EAFVPGEVAA AAGTATLAYV SAALRAARAG QVDAVIACPH SETAINASGV RFAGYPGFVA HEMGMPAEDV YLLLIGGGLR IVHATLHEGI ASALARLDQR HVERAARAAV QALQLMGIAH PVVGLMGINP HAGEGGLFGR DDIDITEPVA RKLRDDGMTV IGPQGADLLL TNPDIDVFVA MYHDQGHIPV KLRAGRHSAA LSIGAGVLFS SVGHGSGFDI AGTLLADPAP LLGAIRLVTT GTVLAPLAE
NT Sequence
ATGAGCCGCA CCCGTGACCG CGCGATGACT GTCGCGCTGG CGATCGGCGA CCCCAACGGC ATCGGTCCGG AAATCGCGGT CAAGGCTGCC GCCCAGATGG CGCGCGACGA ACGCAGCGAA CATGGCGGCC ACGCTGTGCA CTCGCAGCCG CGCATCGTCC TGTTTGGCGA CGCGTTTGTG ATCCGGCGCT ACGCTCGCCA GTGTTGTCCC GAACTCATGC TTCGCCTCGT GCAACTGGAC GATCTGCCGT CTGCCGAGCC CAACGCAATC GATATGGTCG ACGTCGCGTC GCTGCCTGCC GAAGCGTTTG TGCCAGGCGA GGTCGCGGCC GCCGCGGGCA CGGCAACGCT CGCCTACGTG AGCGCCGCGC TTCGTGCCGC GCGCGCGGGC CAGGTGGACG CCGTGATTGC CTGTCCGCAT TCGGAGACCG CGATCAATGC GTCGGGCGTG CGCTTCGCCG GTTATCCGGG CTTCGTGGCG CACGAAATGG GCATGCCGGC TGAGGACGTC TATCTGCTGT TGATCGGCGG CGGCCTGCGC ATCGTTCATG CGACGCTGCA CGAAGGCATT GCTTCGGCAC TCGCGCGGCT CGATCAGCGC CACGTCGAAC GCGCCGCGCG GGCGGCGGTG CAGGCACTGC AATTGATGGG CATCGCGCAT CCCGTGGTCG GACTGATGGG CATCAATCCG CATGCGGGCG AGGGCGGTCT TTTCGGCCGT GACGACATCG ATATTACCGA GCCGGTCGCG CGCAAGTTGC GTGATGACGG CATGACGGTT ATCGGTCCGC AAGGCGCCGA TCTATTGCTC ACCAATCCCG ACATCGACGT GTTTGTGGCG ATGTATCACG ACCAGGGCCA CATTCCCGTC AAGCTGCGCG CCGGCCGCCA TAGCGCCGCG CTGTCGATCG GCGCGGGCGT GCTGTTTTCG AGCGTCGGGC ACGGCAGCGG TTTCGACATT GCCGGCACCT TGCTCGCGGA TCCCGCGCCG CTGCTCGGCG CGATCCGTCT GGTCACAACT GGAACCGTGC TCGCTCCACT GGCTGAG
Details for BuxeA.00116.a.A1.GE33027
HARVESTED ON: 8/16/2011
SEQUENCED ON: 8/22/2011
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MSRTRDRAMT VALAIGDPNG IGPEIAVKAA AQMARDERSE HGGHAVHSQP RIVLFGDAFV IRRYARQCCP ELMLRLVQLD DLPSAEPNAI DMVDVASLPA EAFVPGEVAA AAGTATLAYV SAALRAARAG QVDAVIACPH SETAINASGV RFAGYPGFVA HEMGMPAEDV YLLLIGGGLR IVHATLHEGI ASALARLDQR HVERAARAAV QALQLMGIAH PVVGLMGINP HAGEGGLFGR DDIDITEPVA RKLRDDGMTV IGPQGADLLL TNPDIDVFVA MYHDQGHIPV KLRAGRHSAA LSIGAGVLFS SVGHGSGFDI AGTLLADPAP LLGAIRLVTT GTVLAPLA
Validated NT Sequence
tcagccagtg gagcgagcac ggttccagtt gtgaccagac ggatcgcgcc gagcagcggc gcgggatccg cgagcaaggt gccggcaatg tcgaaaccgc tgccgtgccc gacgctcgaa aacagcacgc ccgcgccgat cgacagcgcg gcgctatggc ggccggcgcg cagcttgacg ggaatgtggc cctggtcgtg atacatcgcc acaaacacgt cgatgtcggg attggtgagc aatagatcgg cgccttgcgg accgataacc gtcatgccgt catcacgcaa cttgcgcgcg accggctcgg taatatcgat gtcgtcacgg ccgaaaagac cgccctcgcc cgcatgcgga ttgatgccca tcagtccgac cacgggatgc gcgatgccca tcaattgcag tgcctgcacc gccgcccgcg cggcgcgttc gacgtggcgc tgatcgagcc gcgcgagtgc cgaagcaatg ccttcgtgca gcgtcgcatg aacgatgcgc aggccgccgc cgatcaacag cagatagacg tcctcagccg gcatgcccat ttcgtgcgcc acgaagcccg gataaccggc gaagcgcacg cccgacgcat tgatcgcggt ctccgaatgc ggacaggcaa tcacggcgtc cacctggccc gcgcgcgcgg cacgaagcgc ggcgctcacg taggcgagcg ttgccgtgcc cgcggcggcc gcgacctcgc ctggcacaaa cgcttcggca ggcagcgacg cgacgtcgac catatcgatt gcgttgggct cggcagacgg cagatcgtcc agttgcacga ggcgaagcat gagttcggga caacactggc gagcgtagcg ccggatcaca aacgcgtcgc caaacaggac gatgcgcggc tgcgagtgca cagcgtggcc gccatgttcg ctgcgttcgt cgcgcgccat ctgggcggca gccttgaccg cgatttccgg accgatgccg ttggggtcgc cgatcgccag cgcgacagtc atcgcgcggt cacgggtgcg gctcatcgaa ccaggaccct gggtctgagc ttccaggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSRTRDRAM TVALAIGDPN GIGPEIAVKA AAQMARDERS EHGGHAVHSQ PRIVLFGDAF VIRRYARQCC PELMLRLVQL DDLPSAEPNA IDMVDVASLP AEAFVPGEVA AAAGTATLAY VSAALRAARA GQVDAVIACP HSETAINASG VRFAGYPGFV AHEMGMPAED VYLLLIGGGL RIVHATLHEG IASALARLDQ RHVERAARAA VQALQLMGIA HPVVGLMGIN PHAGEGGLFG RDDIDITEPV ARKLRDDGMT VIGPQGADLL LTNPDIDVFV AMYHDQGHIP VKLRAGRHSA ALSIGAGVLF SSVGHGSGFD IAGTLLADPA PLLGAIRLVT TGTVLAPLAE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagccgcacc cgtgaccgcg cgatgactgt cgcgctggcg atcggcgacc ccaacggcat cggtccggaa atcgcggtca aggctgccgc ccagatggcg cgcgacgaac gcagcgaaca tggcggccac gctgtgcact cgcagccgcg catcgtcctg tttggcgacg cgtttgtgat ccggcgctac gctcgccagt gttgtcccga actcatgctt cgcctcgtgc aactggacga tctgccgtct gccgagccca acgcaatcga tatggtcgac gtcgcgtcgc tgcctgccga agcgtttgtg ccaggcgagg tcgcggccgc cgcgggcacg gcaacgctcg cctacgtgag cgccgcgctt cgtgccgcgc gcgcgggcca ggtggacgcc gtgattgcct gtccgcattc ggagaccgcg atcaatgcgt cgggcgtgcg cttcgccggt tatccgggct tcgtggcgca cgaaatgggc atgccggctg aggacgtcta tctgctgttg atcggcggcg gcctgcgcat cgttcatgcg acgctgcacg aaggcattgc ttcggcactc gcgcggctcg atcagcgcca cgtcgaacgc gccgcgcggg cggcggtgca ggcactgcaa ttgatgggca tcgcgcatcc cgtggtcgga ctgatgggca tcaatccgca tgcgggcgag ggcggtcttt tcggccgtga cgacatcgat attaccgagc cggtcgcgcg caagttgcgt gatgacggca tgacggttat cggtccgcaa ggcgccgatc tattgctcac caatcccgac atcgacgtgt ttgtggcgat gtatcacgac cagggccaca ttcccgtcaa gctgcgcgcc ggccgccata gcgccgcgct gtcgatcggc gcgggcgtgc tgttttcgag cgtcgggcac ggcagcggtt tcgacattgc cggcaccttg ctcgcggatc ccgcgccgct gctcggcgcg atccgtctgg tcacaactgg aaccgtgctc gctccactgg ctgagtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa