BuxeA.00113.c

3-oxoacyl-[acyl-carrier-protein] synthase II

CENTER ID: BuxeA.00113.c
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00113.c.A1.GE33026 Full length( BuxeA.00113.c ) 1 428
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.3499
RefSeq: YP_559922.1
UniProt: Q13VL9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAAVAFAFVI YEKGVTVSRR RVVVTGLGLI SPVGNNVADG WANLVAGKSG IANITKFDAS NFSTRFAGEV KGFNIEDYIP GKEARHMDTF IHYGVAAGIQ AMQDSGLEVT DENSERIGVV VGSGIGGLPM IEVTQTELLN RGPRRISPFF VPASIINMIS GHLSIKFGIK GPNLAIVTAC TTGLHCIGEA SRLIEYGDAD VMIAGGAEST VSPLGVGGFA AARALSQRND DPATASRPWD KDRDGFVLGE GAGVMVLEEY EHAKARGAKI YAEVSGYGMS GDAYHMTAPL EDGDGARRCM LAALKNAGIN LDQVNYLNAH GTSTPLGDLA ETTGIKRAFG DHAKDMVVNS TKSMTGHLLG GAGGLESVFT VLAVHHQVSP PTINIFNQDP ACDLDYCANT AREMKIDVAL KNSFGFGGTN GTLVFKRA
NT Sequence
GTGGCCGCTG TGGCTTTTGC TTTTGTCATC TATGAAAAAG GGGTTACCGT GAGCCGCCGT CGTGTTGTTG TTACAGGCCT GGGGCTGATT TCGCCTGTTG GCAATAATGT TGCCGACGGC TGGGCCAATC TGGTCGCCGG CAAGTCGGGT ATTGCCAATA TCACGAAGTT CGATGCGTCG AACTTTTCGA CTCGTTTTGC CGGCGAGGTG AAGGGCTTCA ATATCGAGGA CTACATCCCC GGTAAGGAAG CGCGCCACAT GGATACGTTT ATCCACTACG GCGTGGCTGC AGGCATCCAG GCGATGCAGG ACAGCGGCCT CGAAGTCACC GACGAGAATT CGGAGCGTAT CGGCGTGGTG GTTGGTTCGG GCATCGGCGG CTTGCCGATG ATCGAAGTGA CGCAAACCGA ACTGCTCAAT CGCGGCCCGC GCCGTATTTC GCCGTTCTTC GTGCCGGCGT CGATCATCAA CATGATCTCC GGCCATCTGT CGATCAAGTT CGGCATCAAG GGTCCGAATC TGGCTATCGT CACGGCCTGT ACCACGGGCC TGCACTGTAT CGGCGAGGCG TCGCGCCTGA TCGAATACGG CGACGCGGAC GTGATGATCG CGGGTGGTGC AGAATCGACC GTATCGCCGC TCGGCGTCGG CGGCTTTGCG GCGGCGCGCG CGCTGTCGCA ACGCAATGAC GATCCGGCCA CGGCGAGCCG TCCGTGGGAC AAGGATCGCG ACGGTTTCGT GCTGGGCGAG GGCGCCGGCG TCATGGTGCT CGAAGAGTAC GAGCACGCGA AGGCACGCGG CGCGAAGATT TACGCCGAAG TCAGCGGCTA TGGGATGAGC GGCGACGCCT ATCACATGAC CGCCCCGCTG GAAGACGGCG ACGGCGCGCG CCGCTGCATG CTGGCGGCGT TGAAGAACGC AGGCATCAAC CTCGATCAGG TGAATTATCT GAACGCGCAT GGCACGTCCA CGCCGCTCGG CGACCTGGCG GAAACCACCG GCATCAAGCG CGCCTTTGGC GATCATGCCA AAGACATGGT CGTGAACTCG ACCAAGTCGA TGACCGGGCA CCTGCTGGGC GGCGCCGGCG GTCTGGAGTC GGTGTTTACC GTGCTGGCCG TGCATCATCA GGTGTCGCCG CCGACGATCA ACATCTTCAA CCAGGATCCG GCCTGTGATC TGGATTACTG CGCCAATACC GCGCGGGAGA TGAAGATCGA CGTCGCGCTG AAGAACTCGT TCGGGTTCGG CGGCACGAAC GGCACGCTGG TCTTCAAACG CGCC
Details for BuxeA.00113.c.A1.GE33026
HARVESTED ON: 8/16/2011
SEQUENCED ON: 8/22/2011
EXPECTED MW: 47kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 74
PERCENT COVERAGE: 93
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVAAVAFAF VIYEKGVTVS RRRVVVTGLG LISPVGNNVA DGWANLVAGK SGIANITKFD ASNFSTRFAG EVKGFNIEDY IPGKEARHMD TFIHYGVAAG IQAMQDSGLE VTDENSERIG VVVGSGIGGL PMIEVTQTEL LNRGPRRISP FFVPASIINM ISGHLSIKFG IKGPNLAIVT ACTTGLHCIG EASRLIEYGD ADVMIAGGAE STVSPLGVGG FAAARALSQR XDDPATASRP WDKDRDGFVL GEGAGVMVLE EYXHAKARGA KIYAEXSGYG MSGDAYHMTA PLXXAXXRAA ACWRRXXRXX NLDRXXSEXX WXVXXXRRXG XTXXIXXXXX XXXXXXXXXS XXXXXXXXXX XXXXXXXXXX XXXXGXXXXX XXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg ccgctgtggc ttttgctttt gtcatctatg aaaaaggggt taccgtgagc cgccgtcgtg ttgttgttac aggcctgggg ctgatttcgc ctgttggcaa taatgttgcc gacggctggg ccaatctggt cgccggcaag tcgggtattg ccaatatcac gaagttcgat gcgtcgaact tttcgactcg ttttgccggc gaggtgaagg gcttcaatat cgaggactac atccccggta aggaagcgcg ccacatggat acgtttatcc actacggcgt ggctgcaggc atccaggcga tgcaggacag cggcctcgaa gtcaccgacg agaattcgga gcgtatcggc gtggtggttg gttcgggcat cggcggcttg ccgatgatcg aagtgacgca aaccgaactg ctcaatcgcg gcccgcgccg tatttcgccg ttcttcgtgc cggcgtcgat catcaacatg atctccggcc atctgtcgat caagttcggc atcaagggtc cgaatctggc tatcgtcacg gcctgtacca cgggcctgca ctgtatcggc gaggcgtcgc gcctgatcga atacggcgac gcggacgtga tgatcgcggg tggtgcagaa tcgaccgtat cgccgctcgg cgtcggcggc tttgcggcgg cgcgcgcgct gtcgcaacgc antgacgatc cggccacggc gagccgtccg tgggacaagg atcgcgacgg tttcgtgctg ggcgagggcg ccggcgtcat ggtgctcgaa gagtacnagc acgcgaaggc acgcggcgcg aagatttacg ccgaantcag cggctatggg atgagcggcg acgcctatca catgaccgcc ccgctgnana nggcganggn gcgcgccgct gcatgctggc ggcgttnana acgcnnnntc aacctcgatc gnngantntc tgaacncnca tggnacgtcc nnnccnctcg gcganctggn gnaacnnncn gcatcaannn nnnncnttng nnnannnnnn nnnaannnnn nnngnantcn annnannnna nnnnnnnnng nnnnnnnnnn gnnnnnnnnn nnnnnnnnnn nnnnttnnnn nnnnnngnnn ngggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncnnnn nnnnnnnnnn nnnnnnnnnc nnnnnnnnnn nggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVAAVAFAF VIYEKGVTVS RRRVVVTGLG LISPVGNNVA DGWANLVAGK SGIANITKFD ASNFSTRFAG EVKGFNIEDY IPGKEARHMD TFIHYGVAAG IQAMQDSGLE VTDENSERIG VVVGSGIGGL PMIEVTQTEL LNRGPRRISP FFVPASIINM ISGHLSIKFG IKGPNLAIVT ACTTGLHCIG EASRLIEYGD ADVMIAGGAE STVSPLGVGG FAAARALSQR NDDPATASRP WDKDRDGFVL GEGAGVMVLE EYEHAKARGA KIYAEVSGYG MSGDAYHMTA PLEDGDGARR CMLAALKNAG INLDQVNYLN AHGTSTPLGD LAETTGIKRA FGDHAKDMVV NSTKSMTGHL LGGAGGLESV FTVLAVHHQV SPPTINIFNQ DPACDLDYCA NTAREMKIDV ALKNSFGFGG TNGTLVFKRA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggccgct gtggcttttg cttttgtcat ctatgaaaaa ggggttaccg tgagccgccg tcgtgttgtt gttacaggcc tggggctgat ttcgcctgtt ggcaataatg ttgccgacgg ctgggccaat ctggtcgccg gcaagtcggg tattgccaat atcacgaagt tcgatgcgtc gaacttttcg actcgttttg ccggcgaggt gaagggcttc aatatcgagg actacatccc cggtaaggaa gcgcgccaca tggatacgtt tatccactac ggcgtggctg caggcatcca ggcgatgcag gacagcggcc tcgaagtcac cgacgagaat tcggagcgta tcggcgtggt ggttggttcg ggcatcggcg gcttgccgat gatcgaagtg acgcaaaccg aactgctcaa tcgcggcccg cgccgtattt cgccgttctt cgtgccggcg tcgatcatca acatgatctc cggccatctg tcgatcaagt tcggcatcaa gggtccgaat ctggctatcg tcacggcctg taccacgggc ctgcactgta tcggcgaggc gtcgcgcctg atcgaatacg gcgacgcgga cgtgatgatc gcgggtggtg cagaatcgac cgtatcgccg ctcggcgtcg gcggctttgc ggcggcgcgc gcgctgtcgc aacgcaatga cgatccggcc acggcgagcc gtccgtggga caaggatcgc gacggtttcg tgctgggcga gggcgccggc gtcatggtgc tcgaagagta cgagcacgcg aaggcacgcg gcgcgaagat ttacgccgaa gtcagcggct atgggatgag cggcgacgcc tatcacatga ccgccccgct ggaagacggc gacggcgcgc gccgctgcat gctggcggcg ttgaagaacg caggcatcaa cctcgatcag gtgaattatc tgaacgcgca tggcacgtcc acgccgctcg gcgacctggc ggaaaccacc ggcatcaagc gcgcctttgg cgatcatgcc aaagacatgg tcgtgaactc gaccaagtcg atgaccgggc acctgctggg cggcgccggc ggtctggagt cggtgtttac cgtgctggcc gtgcatcatc aggtgtcgcc gccgacgatc aacatcttca accaggatcc ggcctgtgat ctggattact gcgccaatac cgcgcgggag atgaagatcg acgtcgcgct gaagaactcg ttcgggttcg gcggcacgaa cggcacgctg gtcttcaaac gcgcctaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa