BuxeA.00113.a

3-oxoacyl-(Acyl-carrier-protein) synthase II

CENTER ID: BuxeA.00113.a
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00113.a.A2.GE33024 Mature protein( BuxeA.00113.a ) 16 408
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.3643
RefSeq: YP_560058.1
UniProt: Q13V83

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKRVVITGMG GVTALGDSWD VIETRLRSGV NAVRRMPEWD YFESLHTRLA CPLPGFTMPA HYPRKKTRSM GPVSMYSVRA SELALADAGL ADDATIKDGR MGVAYGSSSG SVQPIRAFGT MLETGSMSDV TSNSYVQMMP HTTAVNVSLF WDLKGRIIPT SCACASGSQA IGYAYEAIQT GKQTLMLAGG AEELSGPAVA VFDTLYATST RNDEPHLTPR PFDAARDGLV VGEGAATLVL EEYGHAVARG ARIHAEIVGF GCNSDGAHMT QPTAETMALA MQLALRDAQL APEAIAYVNA HGTSTDRGDI AESHATAQTF GAHMPISSLK SYVGHTLGAC GALEAWWTIE MMKRNWYAPT LNLTRVDPAC APLDYIAGTG RQIDAEYVMS NNFAFGGINT SLIFRRVR
NT Sequence
ATGAAGCGCG TCGTCATTAC CGGCATGGGC GGCGTCACGG CATTGGGCGA CAGCTGGGAC GTGATCGAAA CGCGTCTGAG GAGCGGCGTG AACGCCGTGC GGCGCATGCC CGAATGGGAC TACTTCGAGT CGCTGCATAC GCGGCTTGCG TGTCCGCTGC CGGGGTTCAC GATGCCCGCG CATTATCCGC GCAAGAAGAC CCGCTCGATG GGACCGGTCT CGATGTACTC GGTGCGCGCG AGCGAACTGG CGCTCGCCGA CGCGGGCCTC GCCGACGACG CCACCATCAA GGACGGCCGC ATGGGCGTCG CGTACGGTTC GTCGTCGGGC TCGGTGCAGC CGATCCGCGC TTTCGGCACG ATGCTCGAAA CCGGCTCGAT GAGCGACGTC ACCTCCAACA GCTACGTGCA GATGATGCCG CACACCACCG CGGTCAACGT CAGCCTGTTC TGGGATCTGA AAGGGCGGAT CATTCCGACC TCGTGCGCGT GCGCCTCGGG CAGCCAGGCA ATCGGCTATG CGTATGAAGC GATCCAGACC GGCAAGCAGA CGCTGATGCT GGCGGGCGGC GCGGAGGAAC TGTCGGGCCC GGCGGTCGCC GTGTTCGATA CGCTGTACGC CACCAGCACG CGCAACGACG AGCCGCATCT GACGCCGCGT CCGTTCGACG CCGCGCGCGA CGGCCTCGTG GTCGGCGAAG GCGCGGCCAC CCTGGTGCTC GAAGAATACG GGCACGCGGT GGCGCGCGGT GCGCGGATTC ACGCGGAGAT CGTCGGCTTC GGCTGCAATT CGGACGGCGC GCATATGACG CAGCCGACCG CCGAAACAAT GGCGCTCGCG ATGCAACTCG CGCTCAGGGA TGCTCAGCTC GCGCCTGAAG CGATTGCCTA CGTGAATGCG CACGGCACCT CGACGGATCG CGGCGATATT GCCGAAAGCC ACGCGACCGC GCAGACCTTC GGCGCGCACA TGCCGATCAG TTCGCTCAAG AGCTATGTCG GCCATACGCT CGGCGCGTGC GGCGCGCTCG AAGCCTGGTG GACCATCGAG ATGATGAAGC GCAACTGGTA TGCGCCGACC TTGAACCTGA CCCGCGTCGA TCCGGCCTGC GCGCCGCTCG ATTACATCGC CGGCACGGGC CGGCAGATCG ATGCCGAATA CGTGATGAGC AACAACTTCG CGTTCGGCGG CATCAACACG TCGCTGATTT TCAGGCGCGT TCGA
Details for BuxeA.00113.a.A2.GE33024
HARVESTED ON: 8/16/2011
SEQUENCED ON: 8/22/2011
EXPECTED MW: 44kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 81
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGDSWDVIE TRLRSGVNAV RRMPEWDYFE SLHTRLACPL PGFTMPAHYP RKKTRSMGPV SMYSVRASEL ALADAGLADD ATIKDGRMGV AYGSSSGSVQ PIRAFGTMLE TGSMSDVTSN SYVQMMPHTT AVNVSLFWDL KGRIIPTSCA CASGSQAIGY AYEAIQTGKQ TLMLAGGAEE LSGPAVAVFD TLYATSTRND EPHLTPRPFD AARDGLVVGE XAATLVLEEY GHAVARGARI HAEIVGFGCN SDGAHMTQPT AETMALAMQL ALRDAQLAPX AIXYVNXHGT STXRGXXAES HATAQXFGAH MPISSLXXYV XYAXXXXALX XXXXXXMXXX LXXXXXXXXX XXXXXXXXXX XXGXXXXXXX XXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
ttttntttac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgggcga cagctgggac gtgatcgaaa cgcgtctgag gagcggcgtg aacgccgtgc ggcgcatgcc cgaatgggac tacttcgagt cgctgcatac gcggcttgcg tgtccgctgc cggggttcac gatgcccgcg cattatccgc gcaagaagac ccgctcgatg ggaccggtct cgatgtactc ggtgcgcgcg agcgaactgg cgctcgccga cgcgggcctc gccgacgacg ccaccatcaa ggacggccgc atgggcgtcg cgtacggttc gtcgtcgggc tcggtgcagc cgatccgcgc tttcggcacg atgctcgaaa ccggctcgat gagcgacgtc acctccaaca gctacgtgca gatgatgccg cacaccaccg cggtcaacgt cagcctgttc tgggatctga aagggcggat cattccgacc tcgtgcgcgt gcgcctcggg cagccaggca atcggctatg cgtatgaagc gatccagacc ggcaagcaga cgctgatgct ggcgggcggc gcggaggaac tgtcgggccc ggcggtcgcc gtgttcgata cgctgtacgc caccagcacg cgcaacgacg agccgcatct gacgccgcgt ccgttcgacg ccgcgcgcga cggcctcgtg gtcggcgaan gcgcggccac cctggtgctc gaagaatacg ggcacgcggt ggcgcgcggt gcgcggattc acgcggagat cgtcggcttc ggctgcaatt cggacggcgc gcatatgacg cagccgaccg ccgaaacaat ggcgctcgcg atgcaactcg cgctcaggga tgctcagctc gcgcctgnag cgattgncta cgtgaatgng cacggcacct cgacgnatcg cggcganntt gccgaaagcc acgcgaccgc gcagancttc ggngcgcaca tgccgatcag ttcgctcnan anctatgtnn nntacgctng nncgngnnnn gcgctcnann ngnnngnnnt cnnnntgatg aannnnnanc tggnannnnn nnnannngnn cnnanccnnn ncnnnnnnnn nnnnnnnncn nnntngnnnn nntnnggnnn nnnnannnnn nnnnaanann nnnnnnnnnn nnnnnntnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGDSWDVIE TRLRSGVNAV RRMPEWDYFE SLHTRLACPL PGFTMPAHYP RKKTRSMGPV SMYSVRASEL ALADAGLADD ATIKDGRMGV AYGSSSGSVQ PIRAFGTMLE TGSMSDVTSN SYVQMMPHTT AVNVSLFWDL KGRIIPTSCA CASGSQAIGY AYEAIQTGKQ TLMLAGGAEE LSGPAVAVFD TLYATSTRND EPHLTPRPFD AARDGLVVGE GAATLVLEEY GHAVARGARI HAEIVGFGCN SDGAHMTQPT AETMALAMQL ALRDAQLAPE AIAYVNAHGT STDRGDIAES HATAQTFGAH MPISSLKSYV GHTLGACGAL EAWWTIEMMK RNWYAPTLNL TRVDPACAPL DYIAGTGRQI DAEYVMSNNF AFGGINTSLI FRRVR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcgacagc tgggacgtga tcgaaacgcg tctgaggagc ggcgtgaacg ccgtgcggcg catgcccgaa tgggactact tcgagtcgct gcatacgcgg cttgcgtgtc cgctgccggg gttcacgatg cccgcgcatt atccgcgcaa gaagacccgc tcgatgggac cggtctcgat gtactcggtg cgcgcgagcg aactggcgct cgccgacgcg ggcctcgccg acgacgccac catcaaggac ggccgcatgg gcgtcgcgta cggttcgtcg tcgggctcgg tgcagccgat ccgcgctttc ggcacgatgc tcgaaaccgg ctcgatgagc gacgtcacct ccaacagcta cgtgcagatg atgccgcaca ccaccgcggt caacgtcagc ctgttctggg atctgaaagg gcggatcatt ccgacctcgt gcgcgtgcgc ctcgggcagc caggcaatcg gctatgcgta tgaagcgatc cagaccggca agcagacgct gatgctggcg ggcggcgcgg aggaactgtc gggcccggcg gtcgccgtgt tcgatacgct gtacgccacc agcacgcgca acgacgagcc gcatctgacg ccgcgtccgt tcgacgccgc gcgcgacggc ctcgtggtcg gcgaaggcgc ggccaccctg gtgctcgaag aatacgggca cgcggtggcg cgcggtgcgc ggattcacgc ggagatcgtc ggcttcggct gcaattcgga cggcgcgcat atgacgcagc cgaccgccga aacaatggcg ctcgcgatgc aactcgcgct cagggatgct cagctcgcgc ctgaagcgat tgcctacgtg aatgcgcacg gcacctcgac ggatcgcggc gatattgccg aaagccacgc gaccgcgcag accttcggcg cgcacatgcc gatcagttcg ctcaagagct atgtcggcca tacgctcggc gcgtgcggcg cgctcgaagc ctggtggacc atcgagatga tgaagcgcaa ctggtatgcg ccgaccttga acctgacccg cgtcgatccg gcctgcgcgc cgctcgatta catcgccggc acgggccggc agatcgatgc cgaatacgtg atgagcaaca acttcgcgtt cggcggcatc aacacgtcgc tgattttcag gcgcgttcga taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa