BuxeA.00109.a

Putative amidohydrolase

CENTER ID: BuxeA.00109.a
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00109.a.B1.GE39504 Full length( BuxeA.00109.a ) 1 442
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.8571
RefSeq: YP_556034.1
UniProt: Q13GV5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSATFFAVPD VRRYALRNVR VPACTTAQPA HSGDFVEADL LIEDGRIAAV APAGTVPAAD GPDLGRSLVL PGMIDCHTHL DKGHIWPRQP NPTGDVPGAA HATGVDRTAR WNAEDVRRRM EFGLMTAWAH GVVAIRTHLD CLAPQAAISL PVLEALRDAW AGRIALQANV LVPLDVYLTD EARALADAAQ RAGATLGAVA RFRTLPNHPI TPQFDVAMTR LLRLASERGL DVDLHVDEST DPTSRMLPEV ARIVRRERFD GRVLCGHCCS LALQDERYVD ATLDALAAAR IDIVSLPTVN LYLQSRAPGK TPTWRGVTLL HEMRARGLRV AVAGDNCRDP FYAYGDHDML DTFTQAVRIL QLDHPIGDWL AAATRTPAQI MGLEAGPRGV VAAGEPADVI VTRARDYSEL LSRHQFDRVV LRAGRPIDTT LPDYRLLDDL MV
NT Sequence
TTGAGCGCGA CCTTCTTTGC CGTGCCGGAC GTGCGCCGCT ACGCGCTGCG CAACGTGCGC GTGCCCGCTT GCACGACCGC GCAGCCCGCG CATAGCGGCG ATTTCGTCGA AGCCGATCTG CTGATCGAAG ACGGCCGCAT TGCCGCCGTC GCGCCGGCCG GAACCGTGCC GGCGGCCGAC GGACCCGACC TCGGCCGGTC GCTCGTGCTG CCGGGCATGA TCGATTGCCA CACGCACCTC GACAAAGGCC ACATCTGGCC GCGCCAGCCG AACCCCACCG GCGACGTGCC GGGCGCTGCG CACGCGACCG GCGTGGACCG CACGGCGCGC TGGAACGCGG AGGACGTGCG GCGGCGTATG GAGTTCGGCC TGATGACCGC GTGGGCGCAC GGGGTGGTCG CCATCCGCAC CCACCTCGAT TGCCTCGCGC CGCAGGCCGC GATCTCGCTG CCGGTGCTCG AAGCGCTGCG CGACGCGTGG GCCGGCCGCA TCGCGCTGCA GGCGAACGTG CTCGTTCCGC TCGACGTCTA CCTGACCGAC GAGGCCCGCG CGCTCGCCGA CGCCGCGCAA CGCGCGGGCG CGACGCTCGG CGCGGTGGCG CGCTTTCGCA CGCTGCCGAA TCACCCAATC ACACCGCAAT TCGACGTGGC GATGACGCGT CTTTTGCGAC TCGCAAGCGA ACGCGGTCTC GACGTGGACC TGCATGTCGA CGAGTCGACC GATCCCACGA GCCGCATGCT GCCCGAAGTC GCGCGCATCG TGCGGCGCGA GCGTTTCGAC GGACGCGTTC TGTGCGGCCA TTGCTGCTCG CTCGCGTTGC AGGACGAGCG CTATGTCGAC GCGACGCTCG ACGCGCTCGC CGCCGCGCGC ATCGACATCG TGAGCCTGCC GACGGTCAAC CTGTATCTGC AATCGCGCGC GCCGGGCAAG ACGCCGACCT GGCGCGGCGT CACGCTGCTC CACGAAATGC GCGCGCGCGG GCTGCGCGTC GCGGTAGCGG GCGACAACTG CCGCGATCCG TTCTACGCCT ATGGCGATCA CGACATGCTC GACACGTTCA CGCAGGCGGT GCGCATTCTG CAGCTCGATC ACCCGATCGG CGACTGGCTC GCGGCAGCCA CGCGCACGCC CGCGCAGATC ATGGGTCTCG AAGCCGGGCC GCGCGGCGTC GTCGCCGCAG GCGAGCCGGC GGACGTCATC GTCACGCGCG CCCGCGACTA TTCGGAGTTG CTGTCGAGGC ATCAGTTCGA CCGCGTCGTG CTGCGCGCCG GCAGGCCGAT CGACACGACG CTGCCCGACT ACCGCCTGCT CGACGACCTG ATGGTC
Details for BuxeA.00109.a.B1.GE39504
HARVESTED ON: 9/10/2015
SEQUENCED ON: 9/16/2015
EXPECTED MW: 49kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS ATFFAVPDVR RYALRNVRVP ACTTAQPAHS GDFVEADLLI EDGRIAAVAP AGTVPAADGP DLGRSLVLPG MIDCHTHLDK GHIWPRQPNP TGDVPGAAHA TGVDRTARWN AEDVRRRMEF GLMTAWAHGV VAIRTHLDCL APQAAISLPV LEALRDAWAG RIALQANVLV PLDVYLTDEA RALADAAQRA GATLGAVARF RTLPNHPITP QFDVAMTRLL RLASERGLDV DLHVDESTDP TSRMLPEVAR IVRRERFDGR VLCGHCCSLA LQDERYVDAT LDALAAARID IVSLPTVNLY LQSRAPGKTP TWRGVTLLHE MRARGLRVAV AGDNCRDPFY AYGDHDMLDT FTQAVRILQL DHPIGDWLAA ATRTPAQIMG LEAGPRGVVA AGEPADVIVT RARDYSELLS RHQFDRVVLR AGRPIDTTLP DYRLLDDLMV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgagcg cgaccttctt tgccgtgccg gacgtgcgcc gctacgcgct gcgcaacgtg cgcgtgcccg cttgcacgac cgcgcagccc gcgcatagcg gcgatttcgt cgaagccgat ctgctgatcg aagacggccg cattgccgcc gtcgcgccgg ccggaaccgt gccggcggcc gacggacccg acctcggccg gtcgctcgtg ctgccgggca tgatcgattg ccacacgcac ctcgacaaag gccacatctg gccgcgccag ccgaacccca ccggcgacgt gccgggcgct gcgcacgcga ccggcgtgga ccgcacggcg cgctggaacg cggaggacgt gcggcggcgt atggagttcg gcctgatgac cgcgtgggcg cacggggtgg tcgccatccg cacccacctc gattgcctcg cgccgcaggc cgcgatctcg ctgccggtgc tcgaagcgct gcgcgacgcg tgggccggcc gcatcgcgct gcaggcgaac gtgctcgttc cgctcgacgt ctacctgacc gacgaggccc gcgcgctcgc cgacgccgcg caacgcgcgg gcgcgacgct cggcgcggtg gcgcgctttc gcacgctgcc gaatcaccca atcacaccgc aattcgacgt ggcgatgacg cgtcttttgc gactcgcaag cgaacgcggt ctcgacgtgg acctgcatgt cgacgagtcg accgatccca cgagccgcat gctgcccgaa gtcgcgcgca tcgtgcggcg cgagcgtttc gacggacgcg ttctgtgcgg ccattgctgc tcgctcgcgt tgcaggacga gcgctatgtc gacgcgacgc tcgacgcgct cgccgccgcg cgcatcgaca tcgtgagcct gccgacggtc aacctgtatc tgcaatcgcg cgcgccgggc aagacgccga cctggcgcgg cgtcacgctg ctccacgaaa tgcgcgcgcg cgggctgcgc gtcgcggtag cgggcgacaa ctgccgcgat ccgttctacg cctatggcga tcacgacatg ctcgacacgt tcacgcaggc ggtgcgcatt ctgcagctcg atcacccgat cggcgactgg ctcgcggcag ccacgcgcac gcccgcgcag atcatgggtc tcgaagccgg gccgcgcggc gtcgtcgccg caggcgagcc ggcggacgtc atcgtcacgc gcgcccgcga ctattcggag ttgctgtcga ggcatcagtt cgaccgcgtc gtgctgcgcg ccggcaggcc gatcgacacg acgctgcccg actaccgcct gctcgacgac ctgatggtct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat