BuxeA.00107.e

RND efflux pump, membrane fusion protein, CzcB subfamily

CENTER ID: BuxeA.00107.e
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00107.e.A2.GE33022 Mature protein( BuxeA.00107.e ) 37 391
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00107.e.A2.PW33632 Mature protein( BuxeA.00107.e ) 37 391

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.1602
RefSeq: YP_558135.1
UniProt: Q141A6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLSSQKRLTA ALGALAVVGA VAGIGFTHGT TSGPLSAAQA ADAPPPATQV DVAVVLSQTI TDWQSYSGRL EAIDHVEVRP LVAGTIIAVH FKDGALVRKG DPLFTIDPRP YQAEVDRAAA QVAAARARAI YTSTDAARAD RLLADNAIAK RDYDEKQNAS REAAAGVKAA EAALEAAQVN LGYTRITASV TGRVSRAELT VGNVVSTGAN APLLTTLVSV SPIYASFDVD EQTYLQYLGR DRNTKVPVSL GLANEDGYSR KGAVTSVDNR LDTSSGTIRV RATIDNADGA LVPGLYARVK VGGGEPHPAI LIDDAAVGTD QAKKFVLVVD GANKVVYREV QLGGLHDNLR VVTSGLKAGE RIVVNGMMHA KPSDTVSPHM VSMTDAAKSA A
NT Sequence
ATGCTCTCCA GTCAAAAACG GCTCACTGCC GCGCTCGGTG CGCTGGCAGT TGTCGGCGCA GTTGCAGGCA TTGGCTTCAC TCATGGGACC ACGTCAGGTC CGCTGAGTGC AGCTCAGGCT GCCGACGCGC CGCCGCCAGC TACTCAGGTC GACGTCGCGG TCGTCCTTTC GCAGACCATC ACGGACTGGC AAAGCTACTC CGGGCGTCTC GAAGCCATCG ACCATGTCGA AGTACGCCCG CTCGTTGCCG GCACCATCAT TGCCGTCCAC TTCAAGGACG GCGCGCTGGT CAGGAAAGGC GACCCGCTTT TCACGATCGA CCCCCGCCCC TATCAGGCCG AAGTCGACCG CGCCGCAGCA CAGGTTGCAG CGGCCAGGGC TCGCGCTATT TACACGTCGA CCGATGCCGC GCGCGCTGAC CGACTGCTCG CCGACAATGC CATCGCAAAG CGCGACTACG ACGAAAAGCA GAATGCCTCG CGCGAAGCTG CCGCAGGAGT GAAGGCTGCC GAAGCCGCGC TGGAAGCAGC ACAGGTCAAT CTCGGCTACA CCAGGATTAC CGCCTCGGTG ACGGGCCGCG TATCGCGCGC CGAACTGACC GTCGGCAACG TCGTATCGAC CGGCGCGAAC GCGCCTCTGC TTACCACGCT GGTTTCGGTG TCGCCGATTT ACGCCTCGTT CGATGTCGAT GAACAGACGT ATCTGCAATA CCTTGGCCGC GACCGCAATA CGAAGGTGCC GGTGTCGCTG GGTCTTGCAA ATGAAGACGG CTACTCGCGC AAGGGGGCGG TTACGTCGGT TGATAACCGG CTCGACACGT CGTCCGGCAC CATCCGTGTG CGTGCCACTA TCGACAACGC AGACGGCGCG CTCGTTCCGG GTCTCTACGC ACGCGTCAAG GTTGGCGGCG GCGAGCCCCA TCCGGCCATT CTGATTGACG ACGCGGCCGT TGGCACTGAC CAGGCGAAGA AGTTCGTGCT GGTCGTGGAC GGCGCGAACA AGGTCGTCTA TCGCGAAGTC CAGCTTGGCG GTCTGCACGA CAACCTCCGC GTCGTGACCT CGGGTCTGAA GGCGGGTGAG CGCATCGTCG TGAACGGCAT GATGCACGCA AAGCCGAGTG ACACCGTCAG CCCGCACATG GTCAGCATGA CAGACGCTGC CAAATCCGCA GCG
Details for BuxeA.00107.e.A2.GE33022
HARVESTED ON: 8/16/2011
SEQUENCED ON: 8/22/2011
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 81
Validated AA Sequence
MAAQAADAPP PATQVDVAVV LSQTITDWQS YSGRLEAIDH VEVRPLVAGT IIAVHFKDGA LVRKGDPLFT IDPRPYQAEV DRAAAQVAAA RARAIYTSTD AARADRLLAD NAIAKRDYDE KQNASREAAA GVKAAEAALE AAQVNLGYTR ITASVTGRVS RAELTVGNVV STGANAPLLT TLVSVSPIYA SFDVDEQTYL QYLGRDRNTK VPVSLGLANE DGYSRKGAVT SVDNRLDTSS GTIRVRATID NADGALVPGL YARVKVGGGE PHPAILIDDA AVGTDXXKKF VLVVDGANKV VYREVQLG
Validated NT Sequence
accgccaagc tggacttcgc gatagacnac cttgttcgcg ccgtccacga ccagcacgaa cttcttcncc nggtcagtgc caacggccgc gtcgtcaatc agaatggccg gatggggctc gccgccgcca accttgacgc gtgcgtagag acccggaacg agcgcgccgt ctgcgttgtc gatagtggca cgcacacgga tggtgccgga cgacgtgtcg agccggttat caaccgacgt aaccgccccc ttgcgcgagt agccgtcttc atttgcaaga cccagcgaca ccggcacctt cgtattgcgg tcgcggccaa ggtattgcag atacgtctgt tcatcgacat cgaacgaggc gtaaatcggc gacaccgaaa ccagcgtggt aagcagaggc gcgttcgcgc cggtcgatac gacgttgccg acggtcagtt cggcgcgcga tacgcggccc gtcaccgagg cggtaatcct ggtgtagccg agattgacct gtgctgcttc cagcgcggct tcggcagcct tcactcctgc ggcagcttcg cgcgaggcat tctgcttttc gtcgtagtcg cgctttgcga tggcattgtc ggcgagcagt cggtcagcgc gcgcggcatc ggtcgacgtg taaatagcgc gagccctggc cgctgcaacc tgtgctgcgg cgcggtcgac ttcggcctga taggggcggg ggtcgatcgt gaaaagcggg tcgcctttcc tgaccagcgc gccgtccttg aagtggacgg caatgatggt gccggcaacg agcgggcgta cttcgacatg gtcgatggct tcgagacgcc cggagtagct ttgccagtcc gtgatggtct gcgaaaggac gaccgcgacg tcgacctgag tagctggcgg cggcgcgtcg gcagcctgag ctgccatcga accaggaccc tgggtctgag cttccagggt a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAQAADAP PPATQVDVAV VLSQTITDWQ SYSGRLEAID HVEVRPLVAG TIIAVHFKDG ALVRKGDPLF TIDPRPYQAE VDRAAAQVAA ARARAIYTST DAARADRLLA DNAIAKRDYD EKQNASREAA AGVKAAEAAL EAAQVNLGYT RITASVTGRV SRAELTVGNV VSTGANAPLL TTLVSVSPIY ASFDVDEQTY LQYLGRDRNT KVPVSLGLAN EDGYSRKGAV TSVDNRLDTS SGTIRVRATI DNADGALVPG LYARVKVGGG EPHPAILIDD AAVGTDQAKK FVLVVDGANK VVYREVQLGG LHDNLRVVTS GLKAGERIVV NGMMHAKPSD TVSPHMVSMT DAAKSAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcagctcag gctgccgacg cgccgccgcc agctactcag gtcgacgtcg cggtcgtcct ttcgcagacc atcacggact ggcaaagcta ctccgggcgt ctcgaagcca tcgaccatgt cgaagtacgc ccgctcgttg ccggcaccat cattgccgtc cacttcaagg acggcgcgct ggtcaggaaa ggcgacccgc ttttcacgat cgacccccgc ccctatcagg ccgaagtcga ccgcgccgca gcacaggttg cagcggccag ggctcgcgct atttacacgt cgaccgatgc cgcgcgcgct gaccgactgc tcgccgacaa tgccatcgca aagcgcgact acgacgaaaa gcagaatgcc tcgcgcgaag ctgccgcagg agtgaaggct gccgaagccg cgctggaagc agcacaggtc aatctcggct acaccaggat taccgcctcg gtgacgggcc gcgtatcgcg cgccgaactg accgtcggca acgtcgtatc gaccggcgcg aacgcgcctc tgcttaccac gctggtttcg gtgtcgccga tttacgcctc gttcgatgtc gatgaacaga cgtatctgca ataccttggc cgcgaccgca atacgaaggt gccggtgtcg ctgggtcttg caaatgaaga cggctactcg cgcaaggggg cggttacgtc ggttgataac cggctcgaca cgtcgtccgg caccatccgt gtgcgtgcca ctatcgacaa cgcagacggc gcgctcgttc cgggtctcta cgcacgcgtc aaggttggcg gcggcgagcc ccatccggcc attctgattg acgacgcggc cgttggcact gaccaggcga agaagttcgt gctggtcgtg gacggcgcga acaaggtcgt ctatcgcgaa gtccagcttg gcggtctgca cgacaacctc cgcgtcgtga cctcgggtct gaaggcgggt gagcgcatcg tcgtgaacgg catgatgcac gcaaagccga gtgacaccgt cagcccgcac atggtcagca tgacagacgc tgccaaatcc gcagcgtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BuxeA.00107.e.A2.PW33632
PURIFICATION DATe: 11/19/2011
CONCENTRATION: 28.14mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 100µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 81
Protocol Notes
notes unavailable
Validated AA Sequence
MAAQAADAPP PATQVDVAVV LSQTITDWQS YSGRLEAIDH VEVRPLVAGT IIAVHFKDGA LVRKGDPLFT IDPRPYQAEV DRAAAQVAAA RARAIYTSTD AARADRLLAD NAIAKRDYDE KQNASREAAA GVKAAEAALE AAQVNLGYTR ITASVTGRVS RAELTVGNVV STGANAPLLT TLVSVSPIYA SFDVDEQTYL QYLGRDRNTK VPVSLGLANE DGYSRKGAVT SVDNRLDTSS GTIRVRATID NADGALVPGL YARVKVGGGE PHPAILIDDA AVGTDXXKKF VLVVDGANKV VYREVQLG
Validated NT Sequence
accgccaagc tggacttcgc gatagacnac cttgttcgcg ccgtccacga ccagcacgaa cttcttcncc nggtcagtgc caacggccgc gtcgtcaatc agaatggccg gatggggctc gccgccgcca accttgacgc gtgcgtagag acccggaacg agcgcgccgt ctgcgttgtc gatagtggca cgcacacgga tggtgccgga cgacgtgtcg agccggttat caaccgacgt aaccgccccc ttgcgcgagt agccgtcttc atttgcaaga cccagcgaca ccggcacctt cgtattgcgg tcgcggccaa ggtattgcag atacgtctgt tcatcgacat cgaacgaggc gtaaatcggc gacaccgaaa ccagcgtggt aagcagaggc gcgttcgcgc cggtcgatac gacgttgccg acggtcagtt cggcgcgcga tacgcggccc gtcaccgagg cggtaatcct ggtgtagccg agattgacct gtgctgcttc cagcgcggct tcggcagcct tcactcctgc ggcagcttcg cgcgaggcat tctgcttttc gtcgtagtcg cgctttgcga tggcattgtc ggcgagcagt cggtcagcgc gcgcggcatc ggtcgacgtg taaatagcgc gagccctggc cgctgcaacc tgtgctgcgg cgcggtcgac ttcggcctga taggggcggg ggtcgatcgt gaaaagcggg tcgcctttcc tgaccagcgc gccgtccttg aagtggacgg caatgatggt gccggcaacg agcgggcgta cttcgacatg gtcgatggct tcgagacgcc cggagtagct ttgccagtcc gtgatggtct gcgaaaggac gaccgcgacg tcgacctgag tagctggcgg cggcgcgtcg gcagcctgag ctgccatcga accaggaccc tgggtctgag cttccagggt a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAQAADAP PPATQVDVAV VLSQTITDWQ SYSGRLEAID HVEVRPLVAG TIIAVHFKDG ALVRKGDPLF TIDPRPYQAE VDRAAAQVAA ARARAIYTST DAARADRLLA DNAIAKRDYD EKQNASREAA AGVKAAEAAL EAAQVNLGYT RITASVTGRV SRAELTVGNV VSTGANAPLL TTLVSVSPIY ASFDVDEQTY LQYLGRDRNT KVPVSLGLAN EDGYSRKGAV TSVDNRLDTS SGTIRVRATI DNADGALVPG LYARVKVGGG EPHPAILIDD AAVGTDQAKK FVLVVDGANK VVYREVQLGG LHDNLRVVTS GLKAGERIVV NGMMHAKPSD TVSPHMVSMT DAAKSAA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCAGCTCAG GCTGCCGACG CGCCGCCGCC AGCTACTCAG GTCGACGTCG CGGTCGTCCT TTCGCAGACC ATCACGGACT GGCAAAGCTA CTCCGGGCGT CTCGAAGCCA TCGACCATGT CGAAGTACGC CCGCTCGTTG CCGGCACCAT CATTGCCGTC CACTTCAAGG ACGGCGCGCT GGTCAGGAAA GGCGACCCGC TTTTCACGAT CGACCCCCGC CCCTATCAGG CCGAAGTCGA CCGCGCCGCA GCACAGGTTG CAGCGGCCAG GGCTCGCGCT ATTTACACGT CGACCGATGC CGCGCGCGCT GACCGACTGC TCGCCGACAA TGCCATCGCA AAGCGCGACT ACGACGAAAA GCAGAATGCC TCGCGCGAAG CTGCCGCAGG AGTGAAGGCT GCCGAAGCCG CGCTGGAAGC AGCACAGGTC AATCTCGGCT ACACCAGGAT TACCGCCTCG GTGACGGGCC GCGTATCGCG CGCCGAACTG ACCGTCGGCA ACGTCGTATC GACCGGCGCG AACGCGCCTC TGCTTACCAC GCTGGTTTCG GTGTCGCCGA TTTACGCCTC GTTCGATGTC GATGAACAGA CGTATCTGCA ATACCTTGGC CGCGACCGCA ATACGAAGGT GCCGGTGTCG CTGGGTCTTG CAAATGAAGA CGGCTACTCG CGCAAGGGGG CGGTTACGTC GGTTGATAAC CGGCTCGACA CGTCGTCCGG CACCATCCGT GTGCGTGCCA CTATCGACAA CGCAGACGGC GCGCTCGTTC CGGGTCTCTA CGCACGCGTC AAGGTTGGCG GCGGCGAGCC CCATCCGGCC ATTCTGATTG ACGACGCGGC CGTTGGCACT GACCAGGCGA AGAAGTTCGT GCTGGTCGTG GACGGCGCGA ACAAGGTCGT CTATCGCGAA GTCCAGCTTG GCGGTCTGCA CGACAACCTC CGCGTCGTGA CCTCGGGTCT GAAGGCGGGT GAGCGCATCG TCGTGAACGG CATGATGCAC GCAAAGCCGA GTGACACCGT CAGCCCGCAC ATGGTCAGCA TGACAGACGC TGCCAAATCC GCAGCGTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA