BuxeA.00107.c

Membrane fusion protein, RND efflux pump

CENTER ID: BuxeA.00107.c
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00107.c.A2.GE32497 Mature protein( BuxeA.00107.c ) 28 410
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00107.c.A2.PS01316 Mature protein( BuxeA.00107.c ) 28 410

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.3891
RefSeq: YP_560278.1
UniProt: Q13UL3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRVERVPFRL ISAATAAVLL AACGPKQSAP PPQTPEVGVV TVQPTAVPVV TELPGRTSAF LVAQVRARVD GIVLRREFTE GSQVKAGQRL YKIDPAPYIA TLNNAKATLA KAQANLVSTT AQANRYKVLV AANAVSKQDY DNAVAAEGQA AADVAAGKAA VDTAQINLGY TDVVSPVSGQ VGISQVTPGA YVQASAATLM ATVQTLDPVY VDLTQSSLDG LKLRRDMQEG RLKTTGPDAA RVSLILEDGR TYSEKGKLQF TDVTVDQGTG SVTVRAIFKN ADRVLLPGMF VRARIDEGVN DNALVVPQVG VTHDQKGQPT ALVVGNDNKV ALRQLVTSGT YGSNWVVESG LNPGDRVIVQ GTDKAKPGMQ VKAVAAQLPA TPASDASAQG APAASGAAQM AQPASAASGA
NT Sequence
ATGCGCGTCG AACGGGTTCC ATTCCGCTTA ATCAGTGCCG CGACGGCTGC CGTATTGCTG GCAGCGTGCG GACCAAAACA ATCTGCGCCG CCGCCCCAAA CGCCCGAAGT CGGCGTTGTC ACAGTTCAGC CGACGGCCGT CCCCGTCGTC ACCGAATTGC CCGGCCGCAC CAGTGCCTTC CTCGTCGCGC AAGTGCGCGC GCGGGTCGAC GGCATCGTGC TGCGCCGCGA GTTCACGGAA GGCAGCCAGG TCAAGGCGGG TCAGCGTCTG TACAAGATCG ATCCGGCGCC GTACATCGCC ACGTTGAACA ACGCCAAGGC GACGCTGGCG AAGGCGCAGG CCAATCTCGT GTCCACCACG GCGCAGGCCA ATCGCTACAA GGTGCTGGTT GCCGCCAACG CGGTCAGCAA GCAGGACTAC GACAACGCGG TCGCGGCGGA AGGCCAGGCG GCGGCTGACG TCGCAGCGGG CAAGGCGGCG GTCGACACGG CGCAGATCAA TCTGGGCTAC ACCGACGTCG TGTCGCCGGT GAGCGGCCAG GTCGGCATCT CGCAGGTCAC GCCGGGCGCC TACGTGCAAG CCAGCGCCGC CACGCTGATG GCCACCGTGC AGACACTCGA TCCGGTCTAT GTGGACCTGA CGCAATCGAG CCTCGACGGC CTGAAGCTGC GCCGCGACAT GCAGGAAGGG CGCCTGAAAA CGACGGGTCC GGACGCCGCC AGAGTGTCGC TGATTCTCGA AGACGGCCGC ACCTATTCGG AAAAGGGCAA GCTGCAGTTC ACCGACGTGA CGGTCGACCA GGGCACCGGT TCGGTCACGG TGCGCGCGAT CTTCAAGAAC GCCGACCGGG TGCTGCTGCC TGGCATGTTC GTGCGCGCGC GCATCGACGA AGGCGTGAAC GACAACGCGC TCGTGGTGCC GCAAGTCGGC GTGACGCACG ACCAGAAGGG GCAGCCCACC GCGCTGGTCG TCGGTAACGA CAACAAGGTC GCACTGCGTC AACTGGTTAC GTCCGGCACG TATGGTTCGA ACTGGGTGGT CGAAAGCGGC CTGAATCCGG GCGACCGCGT GATCGTGCAG GGCACCGACA AGGCGAAACC CGGCATGCAG GTCAAGGCCG TTGCCGCGCA ACTTCCCGCC ACACCCGCTT CCGACGCCTC CGCTCAGGGC GCGCCGGCCG CCAGCGGTGC TGCGCAGATG GCTCAACCTG CCTCCGCTGC ATCGGGCGCG
Details for BuxeA.00107.c.A2.GE32497
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/11/2011
EXPECTED MW: 41kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MSAPPPQTPE VGVVTVQPTA VPVVTELPGR TSAFLVAQVR ARVDGIVLRR EFTEGSQVKA GQRLYKIDPA PYIATLNNAK ATLAKAQANL VSTTAQANRY KVLVAANAVS KQDYDNAVAA EGQAAADVAA GKAAVDTAQI NLGYTDVVSP VSGQVGISQV TPGAYVQASA ATLMATVQTL DPVYVDLTQS SLDGLKLRRD MQEGRLKTTG PDAARVSLIL EDGRTYSEKG KLQFTDVTVD QGTGSVTVRA IFKNADRVLL PGMFVRARID EGVNDNALVV PQVGVTHDQK GQPTALVVGN DNKVALRQLV TSGTYGSNWV VESGLNPGDR VIVQGTDKAK PGMQVKAVAA QLPATPASDA SAQGAPAASG AAQMAQPASA AS
Validated NT Sequence
cccgatgcag cggaggcagg ttgagccatc tgcgcagcac cgctggcggc cggcgcgccc tgagcggagg cgtcggaagc gggtgtggcg ggaagttgcg cggcaacggc cttgacctgc atgccgggtt tcgccttgtc ggtgccctgc acgatcacgc ggtcgcccgg attcaggccg ctttcgacca cccagttcga accatacgtg ccggacgtaa ccagttgacg cagtgcgacc ttgttgtcgt taccgacgac cagcgcggtg ggctgcccct tctggtcgtg cgtcacgccg acttgcggca ccacgagcgc gttgtcgttc acgccttcgt cgatgcgcgc gcgcacgaac atgccaggca gcagcacccg gtcggcgttc ttgaagatcg cgcgcaccgt gaccgaaccg gtgccctggt cgaccgtcac gtcggtgaac tgcagcttgc ccttttccga ataggtgcgg ccgtcttcga gaatcagcga cactctggcg gcgtccggac ccgtcgtttt caggcgccct tcctgcatgt cgcggcgcag cttcaggccg tcgaggctcg attgcgtcag gtccacatag accggatcga gtgtctgcac ggtggccatc agcgtggcgg cgctggcttg cacgtaggcg cccggcgtga cctgcgagat gccgacctgg ccgctcaccg gcgacacgac gtcggtgtag cccagattga tctgcgccgt gtcgaccgcc gccttgcccg ctgcgacgtc agccgccgcc tggccttccg ccgcgaccgc gttgtcgtag tcctgcttgc tgaccgcgtt ggcggcaacc agcaccttgt agcgattggc ctgcgccgtg gtggacacga gattggcctg cgccttcgcc agcgtcgcct tggcgttgtt caacgtggcg atgtacggcg ccggatcgat cttgtacaga cgctgacccg ccttgacctg gctgccttcc gtgaactcgc ggcgcagcac gatgccgtcg acccgcgcgc gcacttgcgc gacgaggaag gcactggtgc ggccgggcaa ttcggtgacg acggggacgg ccgtcggctg aactgtgaca acgccgactt cgggcgtttg gggcggcggc gcagacatcg aaccaggacc ctgggtctga gcttccaggg tacc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSAPPPQTP EVGVVTVQPT AVPVVTELPG RTSAFLVAQV RARVDGIVLR REFTEGSQVK AGQRLYKIDP APYIATLNNA KATLAKAQAN LVSTTAQANR YKVLVAANAV SKQDYDNAVA AEGQAAADVA AGKAAVDTAQ INLGYTDVVS PVSGQVGISQ VTPGAYVQAS AATLMATVQT LDPVYVDLTQ SSLDGLKLRR DMQEGRLKTT GPDAARVSLI LEDGRTYSEK GKLQFTDVTV DQGTGSVTVR AIFKNADRVL LPGMFVRARI DEGVNDNALV VPQVGVTHDQ KGQPTALVVG NDNKVALRQL VTSGTYGSNW VVESGLNPGD RVIVQGTDKA KPGMQVKAVA AQLPATPASD ASAQGAPAAS GAAQMAQPAS AASGA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctgcgccg ccgccccaaa cgcccgaagt cggcgttgtc acagttcagc cgacggccgt ccccgtcgtc accgaattgc ccggccgcac cagtgccttc ctcgtcgcgc aagtgcgcgc gcgggtcgac ggcatcgtgc tgcgccgcga gttcacggaa ggcagccagg tcaaggcggg tcagcgtctg tacaagatcg atccggcgcc gtacatcgcc acgttgaaca acgccaaggc gacgctggcg aaggcgcagg ccaatctcgt gtccaccacg gcgcaggcca atcgctacaa ggtgctggtt gccgccaacg cggtcagcaa gcaggactac gacaacgcgg tcgcggcgga aggccaggcg gcggctgacg tcgcagcggg caaggcggcg gtcgacacgg cgcagatcaa tctgggctac accgacgtcg tgtcgccggt gagcggccag gtcggcatct cgcaggtcac gccgggcgcc tacgtgcaag ccagcgccgc cacgctgatg gccaccgtgc agacactcga tccggtctat gtggacctga cgcaatcgag cctcgacggc ctgaagctgc gccgcgacat gcaggaaggg cgcctgaaaa cgacgggtcc ggacgccgcc agagtgtcgc tgattctcga agacggccgc acctattcgg aaaagggcaa gctgcagttc accgacgtga cggtcgacca gggcaccggt tcggtcacgg tgcgcgcgat cttcaagaac gccgaccggg tgctgctgcc tggcatgttc gtgcgcgcgc gcatcgacga aggcgtgaac gacaacgcgc tcgtggtgcc gcaagtcggc gtgacgcacg accagaaggg gcagcccacc gcgctggtcg tcggtaacga caacaaggtc gcactgcgtc aactggttac gtccggcacg tatggttcga actgggtggt cgaaagcggc ctgaatccgg gcgaccgcgt gatcgtgcag ggcaccgaca aggcgaaacc cggcatgcag gtcaaggccg ttgccgcgca acttcccgcc acacccgctt ccgacgcctc cgctcagggc gcgccggccg ccagcggtgc tgcgcagatg gctcaacctg cctccgctgc atcgggcgcg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for BuxeA.00107.c.A2.PS01316
PURIFICATION DATe: 12/28/2011
CONCENTRATION: 33.7mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MSAPPPQTPE VGVVTVQPTA VPVVTELPGR TSAFLVAQVR ARVDGIVLRR EFTEGSQVKA GQRLYKIDPA PYIATLNNAK ATLAKAQANL VSTTAQANRY KVLVAANAVS KQDYDNAVAA EGQAAADVAA GKAAVDTAQI NLGYTDVVSP VSGQVGISQV TPGAYVQASA ATLMATVQTL DPVYVDLTQS SLDGLKLRRD MQEGRLKTTG PDAARVSLIL EDGRTYSEKG KLQFTDVTVD QGTGSVTVRA IFKNADRVLL PGMFVRARID EGVNDNALVV PQVGVTHDQK GQPTALVVGN DNKVALRQLV TSGTYGSNWV VESGLNPGDR VIVQGTDKAK PGMQVKAVAA QLPATPASDA SAQGAPAASG AAQMAQPASA AS
Validated NT Sequence
cccgatgcag cggaggcagg ttgagccatc tgcgcagcac cgctggcggc cggcgcgccc tgagcggagg cgtcggaagc gggtgtggcg ggaagttgcg cggcaacggc cttgacctgc atgccgggtt tcgccttgtc ggtgccctgc acgatcacgc ggtcgcccgg attcaggccg ctttcgacca cccagttcga accatacgtg ccggacgtaa ccagttgacg cagtgcgacc ttgttgtcgt taccgacgac cagcgcggtg ggctgcccct tctggtcgtg cgtcacgccg acttgcggca ccacgagcgc gttgtcgttc acgccttcgt cgatgcgcgc gcgcacgaac atgccaggca gcagcacccg gtcggcgttc ttgaagatcg cgcgcaccgt gaccgaaccg gtgccctggt cgaccgtcac gtcggtgaac tgcagcttgc ccttttccga ataggtgcgg ccgtcttcga gaatcagcga cactctggcg gcgtccggac ccgtcgtttt caggcgccct tcctgcatgt cgcggcgcag cttcaggccg tcgaggctcg attgcgtcag gtccacatag accggatcga gtgtctgcac ggtggccatc agcgtggcgg cgctggcttg cacgtaggcg cccggcgtga cctgcgagat gccgacctgg ccgctcaccg gcgacacgac gtcggtgtag cccagattga tctgcgccgt gtcgaccgcc gccttgcccg ctgcgacgtc agccgccgcc tggccttccg ccgcgaccgc gttgtcgtag tcctgcttgc tgaccgcgtt ggcggcaacc agcaccttgt agcgattggc ctgcgccgtg gtggacacga gattggcctg cgccttcgcc agcgtcgcct tggcgttgtt caacgtggcg atgtacggcg ccggatcgat cttgtacaga cgctgacccg ccttgacctg gctgccttcc gtgaactcgc ggcgcagcac gatgccgtcg acccgcgcgc gcacttgcgc gacgaggaag gcactggtgc ggccgggcaa ttcggtgacg acggggacgg ccgtcggctg aactgtgaca acgccgactt cgggcgtttg gggcggcggc gcagacatcg aaccaggacc ctgggtctga gcttccaggg tacc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSAPPPQTP EVGVVTVQPT AVPVVTELPG RTSAFLVAQV RARVDGIVLR REFTEGSQVK AGQRLYKIDP APYIATLNNA KATLAKAQAN LVSTTAQANR YKVLVAANAV SKQDYDNAVA AEGQAAADVA AGKAAVDTAQ INLGYTDVVS PVSGQVGISQ VTPGAYVQAS AATLMATVQT LDPVYVDLTQ SSLDGLKLRR DMQEGRLKTT GPDAARVSLI LEDGRTYSEK GKLQFTDVTV DQGTGSVTVR AIFKNADRVL LPGMFVRARI DEGVNDNALV VPQVGVTHDQ KGQPTALVVG NDNKVALRQL VTSGTYGSNW VVESGLNPGD RVIVQGTDKA KPGMQVKAVA AQLPATPASD ASAQGAPAAS GAAQMAQPAS AASGA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCTGCGCCG CCGCCCCAAA CGCCCGAAGT CGGCGTTGTC ACAGTTCAGC CGACGGCCGT CCCCGTCGTC ACCGAATTGC CCGGCCGCAC CAGTGCCTTC CTCGTCGCGC AAGTGCGCGC GCGGGTCGAC GGCATCGTGC TGCGCCGCGA GTTCACGGAA GGCAGCCAGG TCAAGGCGGG TCAGCGTCTG TACAAGATCG ATCCGGCGCC GTACATCGCC ACGTTGAACA ACGCCAAGGC GACGCTGGCG AAGGCGCAGG CCAATCTCGT GTCCACCACG GCGCAGGCCA ATCGCTACAA GGTGCTGGTT GCCGCCAACG CGGTCAGCAA GCAGGACTAC GACAACGCGG TCGCGGCGGA AGGCCAGGCG GCGGCTGACG TCGCAGCGGG CAAGGCGGCG GTCGACACGG CGCAGATCAA TCTGGGCTAC ACCGACGTCG TGTCGCCGGT GAGCGGCCAG GTCGGCATCT CGCAGGTCAC GCCGGGCGCC TACGTGCAAG CCAGCGCCGC CACGCTGATG GCCACCGTGC AGACACTCGA TCCGGTCTAT GTGGACCTGA CGCAATCGAG CCTCGACGGC CTGAAGCTGC GCCGCGACAT GCAGGAAGGG CGCCTGAAAA CGACGGGTCC GGACGCCGCC AGAGTGTCGC TGATTCTCGA AGACGGCCGC ACCTATTCGG AAAAGGGCAA GCTGCAGTTC ACCGACGTGA CGGTCGACCA GGGCACCGGT TCGGTCACGG TGCGCGCGAT CTTCAAGAAC GCCGACCGGG TGCTGCTGCC TGGCATGTTC GTGCGCGCGC GCATCGACGA AGGCGTGAAC GACAACGCGC TCGTGGTGCC GCAAGTCGGC GTGACGCACG ACCAGAAGGG GCAGCCCACC GCGCTGGTCG TCGGTAACGA CAACAAGGTC GCACTGCGTC AACTGGTTAC GTCCGGCACG TATGGTTCGA ACTGGGTGGT CGAAAGCGGC CTGAATCCGG GCGACCGCGT GATCGTGCAG GGCACCGACA AGGCGAAACC CGGCATGCAG GTCAAGGCCG TTGCCGCGCA ACTTCCCGCC ACACCCGCTT CCGACGCCTC CGCTCAGGGC GCGCCGGCCG CCAGCGGTGC TGCGCAGATG GCTCAACCTG CCTCCGCTGC ATCGGGCGCG TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA