SSGCID: Target Page

BuxeA.00107.a

RND efflux pump, membrane fusion protein, CzcB subfamily

CENTER ID:	BuxeA.00107.a
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00107.a.A2.GE32495	Mature protein( BuxeA.00107.a )		28	418

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00107.a.A2.PW32415	Mature protein( BuxeA.00107.a )		28	418

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.6932
RefSeq:	YP_554455.1
UniProt:	Q13LD4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTILPLSRRR VAIAALVVVV VAGLGTFGAI RVDASSPAAP TIVPEVDVAT VVQKTITDWQ SYSGRLEAVE KVDVRSQVPG TIVSVNFKDG ALVKKGDTLF VIDPRPYAAE VDRAEAQLAA AQARTGYTQS DWERAQRLIA DNAIARRDYD EKQNAAREAS ANLKAAQAAL ETARINLGYT KIVAPVSGRV SRAEITVGNV ISAGATAAPL TTLVSVSPIY ASFDADEQTY LQYLSRAKDG SKVPVELGLA DESGYSRSGV IESVDNRLDT SSGTIRVRAR FDNQDGALIP GLYARVKVGG SEPHPALLID DAAVGTDQDK KFVLVVDQGN HVVYRTIDVG GMQGNLRVVK GGLKPDDRIV VNGIQRVRPG DTVRAHMVPM TADQDPNSAP LAQTPAQPQT QTQAQTQTPT RTKADKNS

NT Sequence

ATGACTATCC TTCCTCTTTC CCGTCGCCGT GTGGCGATCG CCGCACTTGT CGTCGTCGTC GTGGCCGGAC TGGGCACGTT CGGCGCGATT CGCGTGGACG CGAGCTCGCC CGCCGCGCCG ACCATCGTGC CGGAAGTCGA TGTCGCCACT GTGGTGCAGA AAACGATCAC GGACTGGCAG AGCTATTCGG GCCGTCTCGA AGCCGTCGAA AAGGTGGACG TGCGTTCGCA GGTGCCGGGC ACCATCGTGT CCGTCAATTT CAAGGACGGC GCGCTCGTGA AGAAGGGCGA CACGCTGTTC GTGATCGACC CGCGCCCGTA CGCCGCCGAA GTGGATCGCG CCGAAGCGCA ACTCGCCGCC GCGCAGGCGC GCACGGGTTA CACGCAGAGC GATTGGGAGC GTGCGCAACG CCTGATTGCC GACAATGCGA TCGCCAGGCG TGACTACGAC GAGAAGCAGA ACGCCGCGCG CGAAGCGAGC GCGAACCTGA AGGCCGCGCA AGCCGCCCTC GAAACCGCCC GCATCAATCT CGGCTACACG AAGATCGTCG CGCCGGTTTC GGGCCGCGTG TCGCGCGCGG AAATCACGGT GGGCAACGTC ATTTCGGCGG GCGCGACTGC TGCGCCGCTG ACCACGCTGG TGTCGGTTTC GCCGATCTAC GCATCGTTCG ACGCGGACGA ACAGACGTAT CTGCAATACC TGAGCCGCGC GAAAGACGGC AGCAAGGTGC CGGTGGAACT CGGTCTCGCG GACGAAAGCG GCTACTCGCG CAGCGGCGTG ATCGAGTCGG TGGACAACCG CCTCGACACC TCGTCGGGCA CGATCCGCGT GCGCGCGCGC TTCGATAACC AGGATGGTGC ACTGATTCCC GGTTTGTATG CGCGCGTGAA GGTAGGCGGC AGCGAACCGC ATCCGGCGCT GTTGATCGAC GACGCCGCCG TCGGTACCGA TCAGGACAAG AAATTCGTGC TGGTGGTGGA TCAGGGCAAT CACGTCGTGT ATCGCACGAT CGACGTGGGC GGCATGCAGG GCAATCTGCG CGTGGTGAAG GGCGGCCTGA AACCGGATGA CCGCATCGTC GTAAACGGTA TCCAGCGCGT GCGTCCGGGT GACACGGTGC GCGCCCATAT GGTGCCGATG ACGGCCGATC AGGATCCGAA CAGCGCGCCG CTCGCGCAAA CGCCGGCGCA ACCGCAAACG CAGACGCAAG CCCAAACGCA GACGCCGACG CGCACCAAAG CGGACAAGAA TTCG

Details for BuxeA.00107.a.A2.GE32495

HARVESTED ON:	11/8/2011
SEQUENCED ON:	11/11/2011
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

MGAIRVDASS PAAPTIVPEV DVATVVQKTI TDWQSYSGRL EAVEKVDVRS QVPGTIVSVN FKDGALVKKG DTLFVIDPRP YAAEVDRAEA QLAAAQARTG YTQSDWERAQ RLIADNAIAR RDYDEKQNAA REASANLKAA QAALETARIN LGYTKIVAPV SGRVSRAEIT VGNVISAGAT AAPLTTLVSV SPIYASFDAD EQTYLQYLSR AKDGSKVPVE LGLADESGYS RSGVIESVDN RLDTSSGTIR VRARFDNQDG ALIPGLYARV KVGGSEPHPA LLIDDAAVGT DQDKKFVLVV DQGNHVVYRT IDVGGMQGNL RVVKGGLKPD DRIVVNGIQR VRPGDTVRAH MVPMTADQDP NSAPLAQTPA QPQTQTQAQT QTPTR

Validated NT Sequence

tgcgcgtcgg cgtctgcgtt tgggcttgcg tctgcgtttg cggttgcgcc ggcgtttgcg cgagcggcgc gctgttcgga tcctgatcgg ccgtcatcgg caccatatgg gcgcgcaccg tgtcacccgg acgcacgcgc tggataccgt ttacgacgat gcggtcatcc ggtttcaggc cgcccttcac cacgcgcaga ttgccctgca tgccgcccac gtcgatcgtg cgatacacga cgtgattgcc ctgatccacc accagcacga atttcttgtc ctgatcggta ccgacggcgg cgtcgtcgat caacagcgcc ggatgcggtt cgctgccgcc taccttcacg cgcgcataca aaccgggaat cagtgcacca tcctggttat cgaagcgcgc gcgcacgcgg atcgtgcccg acgaggtgtc gaggcggttg tccaccgact cgatcacgcc gctgcgcgag tagccgcttt cgtccgcgag accgagttcc accggcacct tgctgccgtc tttcgcgcgg ctcaggtatt gcagatacgt ctgttcgtcc gcgtcgaacg atgcgtagat cggcgaaacc gacaccagcg tggtcagcgg cgcagcagtc gcgcccgccg aaatgacgtt gcccaccgtg atttccgcgc gcgacacgcg gcccgaaacc ggcgcgacga tcttcgtgta gccgagattg atgcgggcgg tttcgagggc ggcttgcgcg gccttcaggt tcgcgctcgc ttcgcgcgcg gcgttctgct tctcgtcgta gtcacgcctg gcgatcgcat tgtcggcaat caggcgttgc gcacgctccc aatcgctctg cgtgtaaccc gtgcgcgcct gcgcggcggc gagttgcgct tcggcgcgat ccacttcggc ggcgtacggg cgcgggtcga tcacgaacag cgtgtcgccc ttcttcacga gcgcgccgtc cttgaaattg acggacacga tggtgcccgg cacctgcgaa cgcacgtcca ccttttcgac ggcttcgaga cggcccgaat agctctgcca gtccgtgatc gttttctgca ccacagtggc gacatcgact tccggcacga tggtcggcgc ggcgggcgag ctcgcgtcca cgcgaatcgc gcccatcgaa ccaggaccct gggtctgagc ttccagggta cc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMGAIRVDAS SPAAPTIVPE VDVATVVQKT ITDWQSYSGR LEAVEKVDVR SQVPGTIVSV NFKDGALVKK GDTLFVIDPR PYAAEVDRAE AQLAAAQART GYTQSDWERA QRLIADNAIA RRDYDEKQNA AREASANLKA AQAALETARI NLGYTKIVAP VSGRVSRAEI TVGNVISAGA TAAPLTTLVS VSPIYASFDA DEQTYLQYLS RAKDGSKVPV ELGLADESGY SRSGVIESVD NRLDTSSGTI RVRARFDNQD GALIPGLYAR VKVGGSEPHP ALLIDDAAVG TDQDKKFVLV VDQGNHVVYR TIDVGGMQGN LRVVKGGLKP DDRIVVNGIQ RVRPGDTVRA HMVPMTADQD PNSAPLAQTP AQPQTQTQAQ TQTPTRTKAD KNS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcgcgatt cgcgtggacg cgagctcgcc cgccgcgccg accatcgtgc cggaagtcga tgtcgccact gtggtgcaga aaacgatcac ggactggcag agctattcgg gccgtctcga agccgtcgaa aaggtggacg tgcgttcgca ggtgccgggc accatcgtgt ccgtcaattt caaggacggc gcgctcgtga agaagggcga cacgctgttc gtgatcgacc cgcgcccgta cgccgccgaa gtggatcgcg ccgaagcgca actcgccgcc gcgcaggcgc gcacgggtta cacgcagagc gattgggagc gtgcgcaacg cctgattgcc gacaatgcga tcgccaggcg tgactacgac gagaagcaga acgccgcgcg cgaagcgagc gcgaacctga aggccgcgca agccgccctc gaaaccgccc gcatcaatct cggctacacg aagatcgtcg cgccggtttc gggccgcgtg tcgcgcgcgg aaatcacggt gggcaacgtc atttcggcgg gcgcgactgc tgcgccgctg accacgctgg tgtcggtttc gccgatctac gcatcgttcg acgcggacga acagacgtat ctgcaatacc tgagccgcgc gaaagacggc agcaaggtgc cggtggaact cggtctcgcg gacgaaagcg gctactcgcg cagcggcgtg atcgagtcgg tggacaaccg cctcgacacc tcgtcgggca cgatccgcgt gcgcgcgcgc ttcgataacc aggatggtgc actgattccc ggtttgtatg cgcgcgtgaa ggtaggcggc agcgaaccgc atccggcgct gttgatcgac gacgccgccg tcggtaccga tcaggacaag aaattcgtgc tggtggtgga tcagggcaat cacgtcgtgt atcgcacgat cgacgtgggc ggcatgcagg gcaatctgcg cgtggtgaag ggcggcctga aaccggatga ccgcatcgtc gtaaacggta tccagcgcgt gcgtccgggt gacacggtgc gcgcccatat ggtgccgatg acggccgatc aggatccgaa cagcgcgccg ctcgcgcaaa cgccggcgca accgcaaacg cagacgcaag cccaaacgca gacgccgacg cgcaccaaag cggacaagaa ttcgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for BuxeA.00107.a.A2.PW32415

PURIFICATION DATe:	12/24/2011
CONCENTRATION:	31.07mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGCGCGATT CGCGTGGACG CGAGCTCGCC CGCCGCGCCG ACCATCGTGC CGGAAGTCGA TGTCGCCACT GTGGTGCAGA AAACGATCAC GGACTGGCAG AGCTATTCGG GCCGTCTCGA AGCCGTCGAA AAGGTGGACG TGCGTTCGCA GGTGCCGGGC ACCATCGTGT CCGTCAATTT CAAGGACGGC GCGCTCGTGA AGAAGGGCGA CACGCTGTTC GTGATCGACC CGCGCCCGTA CGCCGCCGAA GTGGATCGCG CCGAAGCGCA ACTCGCCGCC GCGCAGGCGC GCACGGGTTA CACGCAGAGC GATTGGGAGC GTGCGCAACG CCTGATTGCC GACAATGCGA TCGCCAGGCG TGACTACGAC GAGAAGCAGA ACGCCGCGCG CGAAGCGAGC GCGAACCTGA AGGCCGCGCA AGCCGCCCTC GAAACCGCCC GCATCAATCT CGGCTACACG AAGATCGTCG CGCCGGTTTC GGGCCGCGTG TCGCGCGCGG AAATCACGGT GGGCAACGTC ATTTCGGCGG GCGCGACTGC TGCGCCGCTG ACCACGCTGG TGTCGGTTTC GCCGATCTAC GCATCGTTCG ACGCGGACGA ACAGACGTAT CTGCAATACC TGAGCCGCGC GAAAGACGGC AGCAAGGTGC CGGTGGAACT CGGTCTCGCG GACGAAAGCG GCTACTCGCG CAGCGGCGTG ATCGAGTCGG TGGACAACCG CCTCGACACC TCGTCGGGCA CGATCCGCGT GCGCGCGCGC TTCGATAACC AGGATGGTGC ACTGATTCCC GGTTTGTATG CGCGCGTGAA GGTAGGCGGC AGCGAACCGC ATCCGGCGCT GTTGATCGAC GACGCCGCCG TCGGTACCGA TCAGGACAAG AAATTCGTGC TGGTGGTGGA TCAGGGCAAT CACGTCGTGT ATCGCACGAT CGACGTGGGC GGCATGCAGG GCAATCTGCG CGTGGTGAAG GGCGGCCTGA AACCGGATGA CCGCATCGTC GTAAACGGTA TCCAGCGCGT GCGTCCGGGT GACACGGTGC GCGCCCATAT GGTGCCGATG ACGGCCGATC AGGATCCGAA CAGCGCGCCG CTCGCGCAAA CGCCGGCGCA ACCGCAAACG CAGACGCAAG CCCAAACGCA GACGCCGACG CGCACCAAAG CGGACAAGAA TTCGTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA