SSGCID: Target Page

BuxeA.00105.a

Putative beta-lactamase class C and other penicillin binding proteins

CENTER ID:	BuxeA.00105.a
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00105.a.B1.GE39503	Full length( BuxeA.00105.a )		1	399

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.4713
RefSeq:	YP_552369.1
UniProt:	Q13SC0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQPAGLCASR LTALTNAMQG YVERGEVAGV VAMVWRRGEI GYFEPLGLRD EAAQLPMERD TLFRIASMTK PVTSAAILML IEEDRLSLDT PVSLWLPELS TPRVLRDPTG PLDQTDPARA SLTVLDLLTH RAGFAYHFTA TGPLSEAYAA AFNGFEARMD GNAWLARIAD LPLMFQPGSR WHYGIATDVL GVLIERVSGM PLGEFFRTRI FEPLGMRDTA FWVPEAQLAR LATAYGVEPG TRRRVVEDHP ASSRWANPDR FQSGGGGLVS TAQDYLQFAQ LLLGRGRVGT TRLLSHRSVD LMRSNFLTRD QRRVPAFGHV QWAGQGFGLG LSIVDDPAQQ LSLGYRSMGS FGWPGAYGTS WFADPVEDLI GLMLIQRRSI EPFPMAIDFE RRVYDSIDD

NT Sequence

ATGCAACCAG CAGGACTTTG CGCGTCTCGG CTGACGGCGC TGACCAACGC CATGCAAGGC TATGTAGAGC GCGGTGAAGT CGCGGGAGTG GTCGCGATGG TCTGGCGGCG CGGCGAGATC GGCTATTTCG AACCGCTTGG CTTGCGAGAT GAAGCCGCGC AGTTGCCGAT GGAGCGCGAC ACGCTGTTCC GCATTGCGTC CATGACCAAG CCGGTGACCA GCGCCGCGAT CCTGATGCTG ATCGAAGAAG ACCGGCTCTC TCTCGATACG CCGGTCTCCC TCTGGTTGCC GGAATTGTCC ACGCCACGTG TGCTGCGCGA TCCCACTGGC CCGCTGGACC AGACCGACCC GGCCAGAGCG TCGCTCACCG TGCTGGATCT GCTGACCCAC CGCGCCGGCT TCGCCTATCA TTTCACGGCA ACCGGACCGC TCTCCGAAGC TTACGCTGCC GCTTTCAACG GTTTCGAAGC GCGCATGGAC GGCAACGCCT GGCTTGCCCG CATCGCGGAT TTGCCGCTGA TGTTTCAGCC CGGCTCACGC TGGCACTACG GCATCGCGAC CGACGTACTC GGCGTGCTGA TCGAACGCGT GAGCGGCATG CCGCTCGGCG AGTTCTTCCG GACCCGGATC TTCGAGCCGC TCGGTATGCG CGACACCGCG TTCTGGGTGC CCGAAGCACA ACTCGCCCGG CTGGCCACTG CTTATGGCGT CGAACCTGGC ACGCGGCGGC GTGTGGTCGA AGATCATCCG GCGTCGAGCC GATGGGCGAA TCCGGACCGG TTCCAGAGCG GCGGCGGCGG CCTGGTCTCG ACCGCGCAGG ACTATCTGCA ATTCGCGCAA CTCCTGCTCG GGCGCGGGCG GGTTGGTACG ACGCGTTTGC TGTCGCATCG CTCGGTGGAC CTGATGCGCT CCAATTTTCT GACGCGCGAC CAACGCCGCG TGCCGGCTTT CGGACATGTG CAGTGGGCAG GCCAGGGGTT CGGCCTCGGC TTGTCCATCG TCGACGATCC GGCGCAGCAG TTGTCGCTCG GCTATCGCTC GATGGGATCG TTCGGCTGGC CGGGCGCATA CGGCACGAGC TGGTTCGCCG ATCCCGTGGA AGACCTGATC GGTCTGATGC TGATCCAGCG GCGCTCCATC GAGCCGTTTC CTATGGCAAT CGATTTCGAA CGCCGCGTGT ACGATTCAAT CGACGAT

Details for BuxeA.00105.a.B1.GE39503

HARVESTED ON:	9/10/2015
SEQUENCED ON:	9/16/2015
EXPECTED MW:	45kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMQ PAGLCASRLT ALTNAMQGYV ERGEVAGVVA MVWRRGEIGY FEPLGLRDEA AQLPMERDTL FRIASMTKPV TSAAILMLIE EDRLSLDTPV SLWLPELSTP RVLRDPTGPL DQTDPARASL TVLDLLTHRA GFAYHFTATG PLSEAYAAAF NGFEARMDGN AWLARIADLP LMFQPGSRWH YGIATDVLGV LIERVSGMPL GEFFRTRIFE PLGMRDTAFW VPEAQLARLA TAYGVEPGTR RRVVEDHPAS SRWANPDRFQ SGGGGLVSTA QDYLQFAQLL LGRGRVGTTR LLSHRSVDLM RSNFLTRDQR RVPAFGHVQW AGQGFGLGLS IVDDPAQQLS LGYRSMGSFG WPGAYGTSWF ADPVEDLIGL MLIQRRSIEP FPMAIDFE

Validated NT Sequence

gcgttcgaaa tcgattgcca taggaaacgg ctcgatggag cgccgctgga tcagcatcag accgatcagg tcttccacgg gatcggcgaa ccagctcgtg ccgtatgcgc ccggccagcc gaacgatccc atcgagcgat agccgagcga caactgctgc gccggatcgt cgacgatgga caagccgagg ccgaacccct ggcctgccca ctgcacatgt ccgaaagccg gcacgcggcg ttggtcgcgc gtcagaaaat tggagcgcat caggtccacc gagcgatgcg acagcaaacg cgtcgtacca acccgcccgc gcccgagcag gagttgcgcg aattgcagat agtcctgcgc ggtcgagacc aggccgccgc cgccgctctg gaaccggtcc ggattcgccc atcggctcga cgccggatga tcttcgacca cacgccgccg cgtgccaggt tcgacgccat aagcagtggc cagccgggcg agttgtgctt cgggcaccca gaacgcggtg tcgcgcatac cgagcggctc gaagatccgg gtccggaaga actcgccgag cggcatgccg ctcacgcgtt cgatcagcac gccgagtacg tcggtcgcga tgccgtagtg ccagcgtgag ccgggctgaa acatcagcgg caaatccgcg atgcgggcaa gccaggcgtt gccgtccatg cgcgcttcga aaccgttgaa agcggcagcg taagcttcgg agagcggtcc ggttgccgtg aaatgatagg cgaagccggc gcggtgggtc agcagatcca gcacggtgag cgacgctctg gccgggtcgg tctggtccag cgggccagtg ggatcgcgca gcacacgtgg cgtggacaat tccggcaacc agagggagac cggcgtatcg agagagagcc ggtcttcttc gatcagcatc aggatcgcgg cgctggtcac cggcttggtc atggacgcaa tgcggaacag cgtgtcgcgc tccatcggca actgcgcggc ttcatctcgc aagccaagcg gttcgaaata gccgatctcg ccgcgccgcc agaccatcgc gaccactccc gcgacttcac cgcgctctac atagccttgc atggcgttgg tcagcgccgt cagccgagac gcgcaaagtc ctgctggttg catatggtgg tggtggtggt gagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaac cagcaggact ttgcgcgtct cggctgacgg cgctgaccaa cgccatgcaa ggctatgtag agcgcggtga agtcgcggga gtggtcgcga tggtctggcg gcgcggcgag atcggctatt tcgaaccgct tggcttgcga gatgaagccg cgcagttgcc gatggagcgc gacacgctgt tccgcattgc gtccatgacc aagccggtga ccagcgccgc gatcctgatg ctgatcgaag aagaccggct ctctctcgat acgccggtct ccctctggtt gccggaattg tccacgccac gtgtgctgcg cgatcccact ggcccgctgg accagaccga cccggccaga gcgtcgctca ccgtgctgga tctgctgacc caccgcgccg gcttcgccta tcatttcacg gcaaccggac cgctctccga agcttacgct gccgctttca acggtttcga agcgcgcatg gacggcaacg cctggcttgc ccgcatcgcg gatttgccgc tgatgtttca gcccggctca cgctggcact acggcatcgc gaccgacgta ctcggcgtgc tgatcgaacg cgtgagcggc atgccgctcg gcgagttctt ccggacccgg atcttcgagc cgctcggtat gcgcgacacc gcgttctggg tgcccgaagc acaactcgcc cggctggcca ctgcttatgg cgtcgaacct ggcacgcggc ggcgtgtggt cgaagatcat ccggcgtcga gccgatgggc gaatccggac cggttccaga gcggcggcgg cggcctggtc tcgaccgcgc aggactatct gcaattcgcg caactcctgc tcgggcgcgg gcgggttggt acgacgcgtt tgctgtcgca tcgctcggtg gacctgatgc gctccaattt tctgacgcgc gaccaacgcc gcgtgccggc tttcggacat gtgcagtggg caggccaggg gttcggcctc ggcttgtcca tcgtcgacga tccggcgcag cagttgtcgc tcggctatcg ctcgatggga tcgttcggct ggccgggcgc atacggcacg agctggttcg ccgatcccgt ggaagacctg atcggtctga tgctgatcca gcggcgctcc atcgagccgt ttcctatggc aatcgatttc gaacgccgcg tgtacgattc aatcgacgat tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat