BuxeA.00102.a

2-dehydro-3-deoxyphosphooctonate aldolase

CENTER ID: BuxeA.00102.a
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00102.a.A1.GE32494 Full length( BuxeA.00102.a ) 1 284
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.2985
RefSeq: YP_559435.1
UniProt: Q13X06

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKLGDFEIGL DRPFFLIAGT CVVESEQMTI DTAGRLKEIC AKLNIPFIYK SSYDKANRSS GKSFRGLGMD EGLRILSEVK RQLGLPVLTD VHAEHEIEQV ASVVDVLQTP AFLCRQTDFI HACARSGKPV NIKKGQFLAP HDMKNVIDKA RDAAREAGLS EDRFMACERG VSFGYNNLVS DMRSLAIMRE TNAPVVFDAT HSVQLPGGQG TSSGGQREFV PVLARAAVAV GVAGLFMETH PNPAQAKSDG PNAVPLHRMA DLLETLVTLD RAVKRAPFLE SDFN
NT Sequence
ATGAAACTGG GCGATTTCGA AATCGGGCTC GACAGGCCGT TTTTCCTGAT CGCGGGCACC TGTGTCGTCG AATCCGAACA GATGACGATC GACACAGCTG GCCGGCTAAA GGAAATCTGC GCGAAGCTGA ATATTCCGTT CATCTACAAG TCGTCGTACG ACAAGGCCAA CCGCAGCAGC GGCAAGTCGT TCCGCGGTCT GGGCATGGAC GAAGGTTTGC GCATCCTGTC GGAAGTGAAG CGCCAACTCG GTCTGCCGGT GCTGACCGAC GTTCATGCCG AGCACGAGAT CGAGCAGGTC GCCTCGGTGG TCGACGTGCT GCAAACGCCG GCTTTCCTGT GCCGTCAAAC GGACTTCATC CACGCTTGCG CACGTTCGGG CAAACCGGTC AACATCAAGA AAGGCCAGTT TCTCGCACCG CACGACATGA AGAACGTGAT CGACAAGGCG CGTGACGCGG CGCGTGAAGC GGGTCTGTCG GAAGACCGGT TCATGGCGTG CGAGCGCGGT GTGTCGTTCG GCTATAACAA CCTCGTGTCG GACATGCGCT CGCTCGCGAT CATGCGCGAA ACCAACGCGC CGGTCGTGTT CGACGCCACT CACTCGGTGC AGTTGCCGGG CGGGCAGGGC ACGAGCTCGG GCGGCCAGCG CGAATTCGTG CCGGTGCTGG CGCGTGCCGC GGTGGCGGTG GGCGTGGCCG GTCTCTTCAT GGAAACGCAC CCGAATCCGG CGCAGGCCAA ATCGGACGGT CCGAACGCCG TGCCGCTGCA TCGCATGGCC GATCTGCTCG AGACGCTGGT CACGCTCGAT CGGGCCGTCA AGCGCGCGCC GTTCCTCGAA AGCGATTTCA AC
Details for BuxeA.00102.a.A1.GE32494
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/11/2011
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 91
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKLGDFEIG LDRPFFLIAG TCVVESEQMT IDTAGRLKEI CAKLNIPFIY KSSYDKANRS SGKSFRGLGM DEGLRILSEV KRQLGLPVLT DVHAEHEIEQ VASVVDVLQT PAFLCRQTDF IHACARSGKP VNIKKGQFLA PHDMKNVIDK ARDAAREAGL SEDRFMACER GVSFGYNNLV SDMRSLAIMR ETNAPVVFDA THSVQLPGGQ GTSSGGQREF VPVLARAAVA VGVAGLFMET HPNPAXAKSD GPNAVPLHRM GRSARDAGHA RSGRQARAVP RKRFX
Validated NT Sequence
tanttttgtt taactttaag aaggagatat accatggctc atcaccatca ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatga aactgggcga tttcgaaatc gggctcgaca ggccgttttt cctgatcgcg ggcacctgtg tcgtcgaatc cgaacagatg acgatcgaca cagctggccg gctaaaggaa atctgcgcga agctgaatat tccgttcatc tacaagtcgt cgtacgacaa ggccaaccgc agcagcggca agtcgttccg cggtctgggc atggacgaag gtttgcgcat cctgtcggaa gtgaagcgcc aactcggtct gccggtgctg accgacgttc atgccgagca cgagatcgag caggtcgcct cggtggtcga cgtgctgcaa acgccggctt tcctgtgccg tcaaacggac ttcatccacg cttgcgcacg ttcgggcaaa ccggtcaaca tcaagaaagg ccagtttctc gcaccgcacg acatgaagaa cgtgatcgac aaggcgcgtg acgcggcgcg tgaagcgggt ctgtcggaag accggttcat ggcgtgcgag cgcggtgtgt cgttcggcta taacaacctc gtgtcggaca tgcgctcgct cgcgatcatg cgcgaaacca acgcgccngt cgtgttcgac gccactcact cggtgcagtt gccgggcggg cagggcacga gctcgggcgg ccagcgcgaa ttcgtgccgg tgctggcgcg tgccgcggtg gcggtgggcg tggccggtct cttcatggaa acgcacccga atccggcgca ngccaaatcg gacggtccga acgccgtgcc gctgcatcgc atgggccgat ctgctcgaga cgctggtcac gctcgatcgg gccgtcaagc gcgcgccgtt cctcgaaagc gatttcanta atancagcac gaacaagttc tgcagcagct tctcgaggat ccngctgcta ncaaancccg aangnaagct ganntggctg ctgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKLGDFEIG LDRPFFLIAG TCVVESEQMT IDTAGRLKEI CAKLNIPFIY KSSYDKANRS SGKSFRGLGM DEGLRILSEV KRQLGLPVLT DVHAEHEIEQ VASVVDVLQT PAFLCRQTDF IHACARSGKP VNIKKGQFLA PHDMKNVIDK ARDAAREAGL SEDRFMACER GVSFGYNNLV SDMRSLAIMR ETNAPVVFDA THSVQLPGGQ GTSSGGQREF VPVLARAAVA VGVAGLFMET HPNPAQAKSD GPNAVPLHRM ADLLETLVTL DRAVKRAPFL ESDFN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaactgggc gatttcgaaa tcgggctcga caggccgttt ttcctgatcg cgggcacctg tgtcgtcgaa tccgaacaga tgacgatcga cacagctggc cggctaaagg aaatctgcgc gaagctgaat attccgttca tctacaagtc gtcgtacgac aaggccaacc gcagcagcgg caagtcgttc cgcggtctgg gcatggacga aggtttgcgc atcctgtcgg aagtgaagcg ccaactcggt ctgccggtgc tgaccgacgt tcatgccgag cacgagatcg agcaggtcgc ctcggtggtc gacgtgctgc aaacgccggc tttcctgtgc cgtcaaacgg acttcatcca cgcttgcgca cgttcgggca aaccggtcaa catcaagaaa ggccagtttc tcgcaccgca cgacatgaag aacgtgatcg acaaggcgcg tgacgcggcg cgtgaagcgg gtctgtcgga agaccggttc atggcgtgcg agcgcggtgt gtcgttcggc tataacaacc tcgtgtcgga catgcgctcg ctcgcgatca tgcgcgaaac caacgcgccg gtcgtgttcg acgccactca ctcggtgcag ttgccgggcg ggcagggcac gagctcgggc ggccagcgcg aattcgtgcc ggtgctggcg cgtgccgcgg tggcggtggg cgtggccggt ctcttcatgg aaacgcaccc gaatccggcg caggccaaat cggacggtcc gaacgccgtg ccgctgcatc gcatggccga tctgctcgag acgctggtca cgctcgatcg ggccgtcaag cgcgcgccgt tcctcgaaag cgatttcaac taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa