SSGCID: Target Page

BuxeA.00098.a

Molybdopterin synthase subunit MoaE

CENTER ID:	BuxeA.00098.a
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00098.a.B1.GE39502	Full length( BuxeA.00098.a )		1	158

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.2149
RefSeq:	YP_558645.1
UniProt:	Q13Z96

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPVRVQTEDF DLTAEVAGLR ARNPKIGAVA CFVGTVRDLN DGSMIETMEL EHYPGMTEKS LEAIIVSARE RWPGIEVLIV HRVGKLYPLD QIVLVATTAA HRGEAFASCE FVMDYLKTQA PFWKKEKTEA GERWVDARVT DDAALARWGI ESDNRTVE

NT Sequence

ATGCCCGTTC GTGTCCAGAC GGAAGACTTC GACCTCACCG CCGAGGTCGC CGGATTGCGC GCGCGCAACC CGAAGATCGG CGCGGTCGCC TGTTTTGTCG GCACCGTGCG CGACCTGAAC GACGGCAGCA TGATCGAGAC GATGGAGCTC GAACACTATC CCGGCATGAC GGAGAAGTCG CTGGAGGCGA TTATCGTCAG CGCGCGTGAA CGCTGGCCGG GCATCGAGGT GCTGATCGTG CACCGGGTCG GCAAGCTGTA TCCGCTCGAT CAGATCGTGC TGGTGGCGAC GACGGCCGCG CATCGCGGGG AAGCCTTCGC GTCGTGCGAA TTCGTGATGG ATTATCTGAA GACCCAGGCG CCGTTCTGGA AGAAAGAGAA GACCGAGGCC GGCGAGCGCT GGGTCGACGC CCGTGTCACC GACGACGCGG CACTCGCCCG CTGGGGCATC GAATCGGACA ATCGGACGGT GGAG

Details for BuxeA.00098.a.B1.GE39502

HARVESTED ON:	data unavailable
SEQUENCED ON:	9/16/2015
EXPECTED MW:	19kDa
OBSERVED MW:	55kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMP VRVQTEDFDL TAEVAGLRAR NPKIGAVACF VGTVRDLXDG SMIETMELEH YPGMTEKSLE AIIVSARERW PGIEVLIVHR VGKLYPLDQI VLVATTAAHR GEAFASCEFV MDYLKTQAPF WKKEKTEAGE RWVDARVTDD AALARWGIEX DNRXVE

Validated NT Sequence

atcttactca cttactccac cgnccgattg tcnnattcga tgccccagcg ggcgagtgcc gcgtcgtcgg tgacacgggc gtcgacccag cgctcgccgg cctcggtctt ctctttcttc cagaacggcg cctgggtctt cagataatcc atcacgaatt cgcacgacgc gaaggcttcc ccgcgatgcg cggccgtcgt cgccaccagc acgatctgat cgagcggata cagcttgccg acccggtgca cgatcagcac ctcgatgccc ggccagcgtt cacgcgcgct gacgataatc gcctccagcg acttctccgt catgccggga tagtgttcga gctccatcgt ctcgatcatg ctgccgtcgn tcaggtcgcg cacggtgccg acaaaacagg cgaccgcgcc gatcttcggg ttgcgcgcgc gcaatccggc gacctcggcg gtgaggtcga agtcttccgt ctggacacga acgggcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

MAHHHHHHMP VRVQTEDFDL TAEVAGLRAR NPKIGAVACF VGTVRDLNDG SMIETMELEH YPGMTEKSLE AIIVSARERW PGIEVLIVHR VGKLYPLDQI VLVATTAAHR GEAFASCEFV MDYLKTQAPF WKKEKTEAGE RWVDARVTDD AALARWGIES DNRTVE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccg ttcgtgtcca gacggaagac ttcgacctca ccgccgaggt cgccggattg cgcgcgcgca acccgaagat cggcgcggtc gcctgttttg tcggcaccgt gcgcgacctg aacgacggca gcatgatcga gacgatggag ctcgaacact atcccggcat gacggagaag tcgctggagg cgattatcgt cagcgcgcgt gaacgctggc cgggcatcga ggtgctgatc gtgcaccggg tcggcaagct gtatccgctc gatcagatcg tgctggtggc gacgacggcc gcgcatcgcg gggaagcctt cgcgtcgtgc gaattcgtga tggattatct gaagacccag gcgccgttct ggaagaaaga gaagaccgag gccggcgagc gctgggtcga cgcccgtgtc accgacgacg cggcactcgc ccgctggggc atcgaatcgg acaatcggac ggtggagtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at