BuxeA.00096.b
8-amino-7-oxononanoate synthase
| CENTER ID: | BuxeA.00096.b |
| ORGANISM: | Burkholderia xenovorans LB400 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuxeA.00096.b.B1.GE39501 | Full length( BuxeA.00096.b ) | 1 | 394 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuxeA.00096.b.B1.PS37838 | Full length( BuxeA.00096.b ) | 1 | 394 |
Structures
5JAY
DEPOSITED: 4/12/2016
DETERMINATION: XRay
CLONE: BuxeA.00096.b.B1.GE39501
PROTEIN: BuxeA.00096.b.B1.PS37838
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|266265.5.peg.209 |
| RefSeq: | YP_556788.1 |
| UniProt: | Q146K3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHLLDTLAEG LKEIDARGLR RRRRTADTPC AAHMTVDGRA IIGFASNDYL GLAAHPQLIA AIAEGAQRYG AGSGGSHLLG GHSRAHAQLE DDLAEFVGGF VENARALYFS TGYMANLATL TALAGRGTTL FSDALNHASL IDGARLSRAD VQIYPHCDTD ALSAMLEASD ADVKVIVSDT VFSMDGDIAP LPRLLELAEQ HGAWLIVDDA HGFGVLGPQG RGAIAQAALR SPNLISIGTL GKAAGVSGAF VAAHETVIEW LVQRARPYIF TTASVPAAAH AVSASLRIIG GEEGDARRAH LQQLIGRTRA MLKATPWLPV DSHTAVQPLI IGANDATLEI AATLDRAGLW VPAIRPPTVP TGTSRLRISL SAAHSQADLD RLEAGLQQLG AKAA
NT Sequence
ATGCATCTGC TCGACACACT CGCCGAAGGG CTGAAGGAGA TTGACGCGCG CGGCCTGCGC CGTCGCCGCC GCACCGCCGA CACGCCGTGC GCGGCCCACA TGACCGTCGA CGGCCGCGCG ATCATCGGCT TCGCCAGCAA CGACTATCTC GGCCTCGCCG CGCATCCGCA ACTGATCGCG GCCATCGCTG AAGGCGCGCA GCGCTATGGC GCGGGCAGCG GCGGCTCGCA TCTGCTCGGC GGCCACTCGC GCGCGCATGC GCAACTCGAA GACGATCTCG CCGAATTCGT CGGCGGCTTC GTCGAGAATG CGCGCGCGCT CTACTTCAGC ACGGGCTACA TGGCGAATCT CGCCACGCTC ACCGCGCTCG CGGGCCGCGG CACGACGCTC TTCTCCGACG CGCTGAATCA CGCGTCGCTG ATCGACGGCG CGCGTTTGTC CCGCGCCGAC GTGCAGATCT ACCCGCACTG CGATACCGAC GCGCTGAGCG CGATGCTCGA AGCCTCGGAC GCCGACGTCA AGGTGATCGT CTCCGACACC GTCTTCAGCA TGGACGGCGA TATCGCGCCG CTCCCACGTC TGCTCGAACT CGCGGAACAA CACGGCGCCT GGCTGATCGT CGACGACGCG CACGGTTTCG GCGTGCTCGG CCCGCAAGGC CGCGGCGCGA TCGCGCAAGC CGCGCTGCGT TCGCCGAATC TCATTTCGAT CGGCACGCTC GGCAAAGCGG CAGGCGTCTC CGGCGCGTTC GTCGCGGCGC ATGAGACGGT GATCGAATGG CTCGTGCAGC GCGCTCGCCC GTACATTTTC ACCACGGCGT CGGTGCCGGC TGCGGCACAT GCGGTGTCGG CGAGTCTGCG CATCATCGGC GGCGAGGAAG GCGATGCGCG GCGCGCGCAT CTTCAGCAAT TGATCGGGCG CACGCGCGCC ATGCTCAAGG CAACGCCGTG GCTACCGGTC GATTCGCATA CGGCCGTGCA GCCGCTCATC ATCGGCGCGA ACGACGCCAC ACTCGAGATC GCGGCCACGC TCGATCGCGC GGGCCTGTGG GTGCCGGCGA TCCGTCCGCC CACCGTGCCC ACCGGCACGT CGCGTTTGCG CATTTCGTTA TCGGCCGCGC ACTCGCAGGC GGATCTGGAT CGTCTTGAAG CAGGCTTGCA GCAACTCGGA GCGAAAGCGG CA
Details for BuxeA.00096.b.B1.GE39501
| HARVESTED ON: | 9/10/2015 |
| SEQUENCED ON: | 9/16/2015 |
| EXPECTED MW: | 42kDa |
| OBSERVED MW: | 42kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 78 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMH LLXXXAEGLX XXDARGLRRR RRTAXXPCAX XMTVXGRAII GFASNDYLGL AAHPQLIAAX AEGAQRYGAG SGGSHLLGGH SRAHXQLXXD LAXFVGGFVE NXRALYFSTG YMANLATLTA LAGRXTTLFS DALNHAXXID GARLSRADVQ IYPHCDTDAL SAMLEASDAD VKVIVSDTVF SMDGDIAPLP RLLELAEQHG AWLIVDDAHG FGVLGPQGRG AIAQAALRSP NLISIGTLDK AAGVSGAFVA AHETVIEWLV QRARPYIFTT ASVPAAAHAV XXXXRIIGGE EXXARRAXXQ QLIGRTRAXX KATPWXXGRF AYGRAAXHHR RXXRHTXDRG HARSXXPVGA XXPSAXRXXX XVXXAXXVXX XAXXGGXXSS
Validated NT Sequence
cntgcttcaa gacgatcnnn ntccgcctnn nnnngcgcnn nnnataacgn nntgcgcaan nnnnacntnn nnnnnnncac gnnnggcgga cggatnnncg gcacccacag gnnnnnncga tcgagcgtgg ccgcgatctn nngtgtggcg tcnnncgcgc cgatgatgnn nggctgcacg gccgtatgcg aatcgaccnn ctnnccacgg cgttgccttg nnnntggcgc gcgtgcgccc gatcaattgc tgannntgcg cgcgccgcgc annnnnttcc tcgccgccga tgatgcgcnn nctnnnnnac accgcatgtg ccgcagccgg caccgacgcc gtggtgaaaa tgtacgggcg agcgcgctgc acgagccatt cgatcaccgt ctcatgcgcc gcgacgaacg cgccggagac gcctgccgct ttgtcgagcg tgccgatcga aatgagattc ggcgaacgca gcgcggcttg cgcgatcgcg ccgcggcctt gcgggccgag cacgccgaaa ccgtgcgcgt cgtcgacgat cagccaggcg ccgtgttgtt ccgcgagttc gagcagacgt gggagcggcg cgatatcgcc gtccatgctg aagacggtgt cggagacgat caccttgacg tcggcgtccg aggcttcgag catcgcgctc agcgcgtcgg tatcgcagtg cgggtagatc tgcacgtcgg cgcgggacaa acgcgcgccg tcgatcannn acgcgtgatt cagcgcgtcg gagaagagcg tcgtnnngcg gcccgcgagc gcggtgagcg tggcgagatt cgccatgtag cccgtgctga agtagagcgc gcgnncattc tcgacgaagc cgccgacgaa tncggcgaga tcgnnnnnna gttgnncatg cgcgcgcgag tggccgccga gcagatgcga gccgccgctg cccgcgccat agcgctgcgc gccttcagcg nnngccgcga tcagttgcgg atgcgcggcg aggccgagat agtcgttgct ggcgaagccg atgatcgcgc ggccgnnnac ggtcatgnnn nccgcgcacg gcntnnnggc ggtgcggcgg cgacggcgca ggccgcgcgc gtcaanntnn ttcagccctt cggcgannnn ntcgagcaga tgcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMH LLDTLAEGLK EIDARGLRRR RRTADTPCAA HMTVDGRAII GFASNDYLGL AAHPQLIAAI AEGAQRYGAG SGGSHLLGGH SRAHAQLEDD LAEFVGGFVE NARALYFSTG YMANLATLTA LAGRGTTLFS DALNHASLID GARLSRADVQ IYPHCDTDAL SAMLEASDAD VKVIVSDTVF SMDGDIAPLP RLLELAEQHG AWLIVDDAHG FGVLGPQGRG AIAQAALRSP NLISIGTLGK AAGVSGAFVA AHETVIEWLV QRARPYIFTT ASVPAAAHAV SASLRIIGGE EGDARRAHLQ QLIGRTRAML KATPWLPVDS HTAVQPLIIG ANDATLEIAA TLDRAGLWVP AIRPPTVPTG TSRLRISLSA AHSQADLDRL EAGLQQLGAK AA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcatc tgctcgacac actcgccgaa gggctgaagg agattgacgc gcgcggcctg cgccgtcgcc gccgcaccgc cgacacgccg tgcgcggccc acatgaccgt cgacggccgc gcgatcatcg gcttcgccag caacgactat ctcggcctcg ccgcgcatcc gcaactgatc gcggccatcg ctgaaggcgc gcagcgctat ggcgcgggca gcggcggctc gcatctgctc ggcggccact cgcgcgcgca tgcgcaactc gaagacgatc tcgccgaatt cgtcggcggc ttcgtcgaga atgcgcgcgc gctctacttc agcacgggct acatggcgaa tctcgccacg ctcaccgcgc tcgcgggccg cggcacgacg ctcttctccg acgcgctgaa tcacgcgtcg ctgatcgacg gcgcgcgttt gtcccgcgcc gacgtgcaga tctacccgca ctgcgatacc gacgcgctga gcgcgatgct cgaagcctcg gacgccgacg tcaaggtgat cgtctccgac accgtcttca gcatggacgg cgatatcgcg ccgctcccac gtctgctcga actcgcggaa caacacggcg cctggctgat cgtcgacgac gcgcacggtt tcggcgtgct cggcccgcaa ggccgcggcg cgatcgcgca agccgcgctg cgttcgccga atctcatttc gatcggcacg ctcggcaaag cggcaggcgt ctccggcgcg ttcgtcgcgg cgcatgagac ggtgatcgaa tggctcgtgc agcgcgctcg cccgtacatt ttcaccacgg cgtcggtgcc ggctgcggca catgcggtgt cggcgagtct gcgcatcatc ggcggcgagg aaggcgatgc gcggcgcgcg catcttcagc aattgatcgg gcgcacgcgc gccatgctca aggcaacgcc gtggctaccg gtcgattcgc atacggccgt gcagccgctc atcatcggcg cgaacgacgc cacactcgag atcgcggcca cgctcgatcg cgcgggcctg tgggtgccgg cgatccgtcc gcccaccgtg cccaccggca cgtcgcgttt gcgcatttcg ttatcggccg cgcactcgca ggcggatctg gatcgtcttg aagcaggctt gcagcaactc ggagcgaaag cggcatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BuxeA.00096.b.B1.PS37838
| PURIFICATION DATe: | 2/17/2016 |
| CONCENTRATION: | 45.7mg/ml |
| OBSERVED MW: | 40kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 78 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMH LLXXXAEGLX XXDARGLRRR RRTAXXPCAX XMTVXGRAII GFASNDYLGL AAHPQLIAAX AEGAQRYGAG SGGSHLLGGH SRAHXQLXXD LAXFVGGFVE NXRALYFSTG YMANLATLTA LAGRXTTLFS DALNHAXXID GARLSRADVQ IYPHCDTDAL SAMLEASDAD VKVIVSDTVF SMDGDIAPLP RLLELAEQHG AWLIVDDAHG FGVLGPQGRG AIAQAALRSP NLISIGTLDK AAGVSGAFVA AHETVIEWLV QRARPYIFTT ASVPAAAHAV XXXXRIIGGE EXXARRAXXQ QLIGRTRAXX KATPWXXGRF AYGRAAXHHR RXXRHTXDRG HARSXXPVGA XXPSAXRXXX XVXXAXXVXX XAXXGGXXSS
Validated NT Sequence
cntgcttcaa gacgatcnnn ntccgcctnn nnnngcgcnn nnnataacgn nntgcgcaan nnnnacntnn nnnnnnncac gnnnggcgga cggatnnncg gcacccacag gnnnnnncga tcgagcgtgg ccgcgatctn nngtgtggcg tcnnncgcgc cgatgatgnn nggctgcacg gccgtatgcg aatcgaccnn ctnnccacgg cgttgccttg nnnntggcgc gcgtgcgccc gatcaattgc tgannntgcg cgcgccgcgc annnnnttcc tcgccgccga tgatgcgcnn nctnnnnnac accgcatgtg ccgcagccgg caccgacgcc gtggtgaaaa tgtacgggcg agcgcgctgc acgagccatt cgatcaccgt ctcatgcgcc gcgacgaacg cgccggagac gcctgccgct ttgtcgagcg tgccgatcga aatgagattc ggcgaacgca gcgcggcttg cgcgatcgcg ccgcggcctt gcgggccgag cacgccgaaa ccgtgcgcgt cgtcgacgat cagccaggcg ccgtgttgtt ccgcgagttc gagcagacgt gggagcggcg cgatatcgcc gtccatgctg aagacggtgt cggagacgat caccttgacg tcggcgtccg aggcttcgag catcgcgctc agcgcgtcgg tatcgcagtg cgggtagatc tgcacgtcgg cgcgggacaa acgcgcgccg tcgatcannn acgcgtgatt cagcgcgtcg gagaagagcg tcgtnnngcg gcccgcgagc gcggtgagcg tggcgagatt cgccatgtag cccgtgctga agtagagcgc gcgnncattc tcgacgaagc cgccgacgaa tncggcgaga tcgnnnnnna gttgnncatg cgcgcgcgag tggccgccga gcagatgcga gccgccgctg cccgcgccat agcgctgcgc gccttcagcg nnngccgcga tcagttgcgg atgcgcggcg aggccgagat agtcgttgct ggcgaagccg atgatcgcgc ggccgnnnac ggtcatgnnn nccgcgcacg gcntnnnggc ggtgcggcgg cgacggcgca ggccgcgcgc gtcaanntnn ttcagccctt cggcgannnn ntcgagcaga tgcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMH LLDTLAEGLK EIDARGLRRR RRTADTPCAA HMTVDGRAII GFASNDYLGL AAHPQLIAAI AEGAQRYGAG SGGSHLLGGH SRAHAQLEDD LAEFVGGFVE NARALYFSTG YMANLATLTA LAGRGTTLFS DALNHASLID GARLSRADVQ IYPHCDTDAL SAMLEASDAD VKVIVSDTVF SMDGDIAPLP RLLELAEQHG AWLIVDDAHG FGVLGPQGRG AIAQAALRSP NLISIGTLGK AAGVSGAFVA AHETVIEWLV QRARPYIFTT ASVPAAAHAV SASLRIIGGE EGDARRAHLQ QLIGRTRAML KATPWLPVDS HTAVQPLIIG ANDATLEIAA TLDRAGLWVP AIRPPTVPTG TSRLRISLSA AHSQADLDRL EAGLQQLGAK AA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCATC TGCTCGACAC ACTCGCCGAA GGGCTGAAGG AGATTGACGC GCGCGGCCTG CGCCGTCGCC GCCGCACCGC CGACACGCCG TGCGCGGCCC ACATGACCGT CGACGGCCGC GCGATCATCG GCTTCGCCAG CAACGACTAT CTCGGCCTCG CCGCGCATCC GCAACTGATC GCGGCCATCG CTGAAGGCGC GCAGCGCTAT GGCGCGGGCA GCGGCGGCTC GCATCTGCTC GGCGGCCACT CGCGCGCGCA TGCGCAACTC GAAGACGATC TCGCCGAATT CGTCGGCGGC TTCGTCGAGA ATGCGCGCGC GCTCTACTTC AGCACGGGCT ACATGGCGAA TCTCGCCACG CTCACCGCGC TCGCGGGCCG CGGCACGACG CTCTTCTCCG ACGCGCTGAA TCACGCGTCG CTGATCGACG GCGCGCGTTT GTCCCGCGCC GACGTGCAGA TCTACCCGCA CTGCGATACC GACGCGCTGA GCGCGATGCT CGAAGCCTCG GACGCCGACG TCAAGGTGAT CGTCTCCGAC ACCGTCTTCA GCATGGACGG CGATATCGCG CCGCTCCCAC GTCTGCTCGA ACTCGCGGAA CAACACGGCG CCTGGCTGAT CGTCGACGAC GCGCACGGTT TCGGCGTGCT CGGCCCGCAA GGCCGCGGCG CGATCGCGCA AGCCGCGCTG CGTTCGCCGA ATCTCATTTC GATCGGCACG CTCGGCAAAG CGGCAGGCGT CTCCGGCGCG TTCGTCGCGG CGCATGAGAC GGTGATCGAA TGGCTCGTGC AGCGCGCTCG CCCGTACATT TTCACCACGG CGTCGGTGCC GGCTGCGGCA CATGCGGTGT CGGCGAGTCT GCGCATCATC GGCGGCGAGG AAGGCGATGC GCGGCGCGCG CATCTTCAGC AATTGATCGG GCGCACGCGC GCCATGCTCA AGGCAACGCC GTGGCTACCG GTCGATTCGC ATACGGCCGT GCAGCCGCTC ATCATCGGCG CGAACGACGC CACACTCGAG ATCGCGGCCA CGCTCGATCG CGCGGGCCTG TGGGTGCCGG CGATCCGTCC GCCCACCGTG CCCACCGGCA CGTCGCGTTT GCGCATTTCG TTATCGGCCG CGCACTCGCA GGCGGATCTG GATCGTCTTG AAGCAGGCTT GCAGCAACTC GGAGCGAAAG CGGCATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT