BuxeA.00096.a

Putative aminotransferase

CENTER ID: BuxeA.00096.a
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00096.a.B1.GE39500 Full length( BuxeA.00096.a ) 1 429
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00096.a.B1.PS37855 Full length( BuxeA.00096.a ) 1 429

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.7794
RefSeq: YP_555293.1
UniProt: Q13IZ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKSVFDRTKQ RHSRPALPAF VTSRVERYYR ERVQETWGGG HIMRGRIAGP DALHLSSNDY LAIARHPDII GSMAATLLEN GNGLLMSGIF LHGDCPQLQF ENRLATFMQA EAGVLCQSGY AANTGLIQSI ASEQTPVYID MMAHMSLWEG VRSAGAVPVA FRHNDAAHLE QQIGRYGQGI VLVDSVYSTT GSVCRLVSLA DVCERHACVF VVDESHSLGT HGPRGAGLVV ELGLQSRVAF RTASLAKAFA GRAGFISCPV DFQEYFKFES NPAIFSSTLL PHEISGLDAT LSVIECADRR RTRLASSAAW LRQRLTELGY NLNGSRSQII ALEAGTEQRT IVLRDALESR GIFGSVFCAP ATARNRALIR LSIHAALSDA QVEKIASVCR DIRSEVELDK WPSTRRLGDE LSARRRQRET ASEETAVTA
NT Sequence
ATGAAAAGCG TCTTTGATCG GACTAAACAG AGGCATAGCC GGCCGGCGTT GCCGGCTTTC GTCACATCGC GCGTCGAGCG GTATTACCGT GAGCGTGTGC AGGAAACATG GGGCGGGGGG CATATCATGC GCGGCCGTAT CGCGGGGCCC GATGCGCTGC ACCTGTCGAG CAACGACTAT CTCGCGATCG CCCGGCACCC GGACATTATC GGCAGCATGG CCGCGACGCT GCTTGAGAAC GGCAACGGGT TGTTGATGTC GGGCATTTTC CTGCACGGCG ATTGCCCTCA ACTCCAGTTC GAAAACCGGC TTGCGACGTT CATGCAGGCG GAGGCGGGCG TGCTGTGCCA GTCGGGCTAC GCGGCCAATA CCGGGCTGAT CCAGTCCATC GCGTCGGAGC AGACGCCGGT CTATATCGAC ATGATGGCGC ACATGTCGTT GTGGGAAGGC GTTCGCAGTG CGGGCGCGGT GCCGGTCGCG TTCCGGCATA ACGACGCCGC GCATCTCGAG CAGCAGATCG GACGCTATGG GCAAGGCATC GTGCTGGTGG ACTCGGTGTA CAGCACGACT GGCAGCGTAT GCCGGCTCGT CTCGCTCGCG GACGTATGCG AACGGCACGC GTGCGTGTTC GTCGTCGACG AGTCGCATTC GCTCGGCACG CACGGTCCGC GTGGCGCAGG GCTCGTCGTC GAACTCGGAC TGCAGTCCCG TGTCGCGTTT CGCACCGCCA GTCTCGCGAA GGCGTTTGCC GGCAGAGCTG GTTTCATTAG CTGTCCGGTC GATTTCCAGG AGTACTTCAA GTTCGAATCG AATCCGGCCA TTTTCAGTTC CACGCTATTG CCGCATGAAA TCAGCGGGCT CGATGCGACA CTGTCGGTGA TCGAATGCGC CGACCGGCGG CGCACGAGAC TCGCGTCGAG CGCCGCATGG CTGAGGCAGC GGTTGACGGA ACTCGGATAC AACCTGAACG GCAGCAGGAG TCAGATCATC GCCCTCGAAG CCGGTACGGA GCAGCGCACG ATCGTGTTGC GCGACGCATT GGAGTCGCGC GGCATTTTCG GCTCGGTGTT CTGTGCGCCC GCGACGGCAC GAAATCGGGC GCTGATCCGC CTGTCGATCC ACGCGGCGCT GAGCGACGCC CAGGTCGAAA AGATTGCCAG CGTCTGCCGT GACATCCGTA GCGAGGTCGA ACTCGACAAG TGGCCGTCCA CGCGACGTCT CGGCGATGAA TTGTCGGCGC GCAGACGCCA ACGCGAGACC GCCAGCGAAG AGACCGCTGT GACGGCT
Details for BuxeA.00096.a.B1.GE39500
HARVESTED ON: 9/10/2015
SEQUENCED ON: 9/16/2015
EXPECTED MW: 48kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMK SVFDRTKQRH SRPALPAFVT SRVERYYRER VQETWGGGHI MRGRIAGPDA LHLSSNDYLA IARHPDIIGS MAATLLENGN GLLMSGIFLH GDCPQLQFEN RLATFMQAEA GVLCQSGYAA NTGLIQSIAS EQTPVYIDMM AHMSLWEGVR SAGAVPVAFR HNDAAHLEQQ IGRYGQGIVL VDSVYSTTGS VCRLVSLADV CERHACVFVV DESHSLGTHG PRGAGLVVEL GLQSRVAFRT ASLAKAFAGR AGFISCPVDF QEYFKFESNP AIFSSTLLPH EISGLDATLS VIECADRRRT RLASSAAWLR QRLTELGYNL NGSRSQIIAL EAGTEQRTIV LRDALESRGI FGSVFCAPAT ARNRALIRLS IHAALSDAQV EKIASVCRDI RSEVELDKWP STRRLGDELS ARRRQRETA
Validated NT Sequence
tggcggtctc gcgttggcgt ctgcgcgccg acaattcatc gccgagacgt cgcgtggacg gccacttgtc gagttcgacc tcgctacgga tgtcacggca gacgctggca atcttttcga cctgggcgtc gctcagcgcc gcgtggatcg acaggcggat cagcgcccga tttcgtgccg tcgcgggcgc acagaacacc gagccgaaaa tgccgcgcga ctccaatgcg tcgcgcaaca cgatcgtgcg ctgctccgta ccggcttcga gggcgatgat ctgactcctg ctgccgttca ggttgtatcc gagttccgtc aaccgctgcc tcagccatgc ggcgctcgac gcgagtctcg tgcgccgccg gtcggcgcat tcgatcaccg acagtgtcgc atcgagcccg ctgatttcat gcggcaatag cgtggaactg aaaatggccg gattcgattc gaacttgaag tactcctgga aatcgaccgg acagctaatg aaaccagctc tgccggcaaa cgccttcgcg agactggcgg tgcgaaacgc gacacgggac tgcagtccga gttcgacgac gagccctgcg ccacgcggac cgtgcgtgcc gagcgaatgc gactcgtcga cgacgaacac gcacgcgtgc cgttcgcata cgtccgcgag cgagacgagc cggcatacgc tgccagtcgt gctgtacacc gagtccacca gcacgatgcc ttgcccatag cgtccgatct gctgctcgag atgcgcggcg tcgttatgcc ggaacgcgac cggcaccgcg cccgcactgc gaacgccttc ccacaacgac atgtgcgcca tcatgtcgat atagaccggc gtctgctccg acgcgatgga ctggatcagc ccggtattgg ccgcgtagcc cgactggcac agcacgcccg cctccgcctg catgaacgtc gcaagccggt tttcgaactg gagttgaggg caatcgccgt gcaggaaaat gcccgacatc aacaacccgt tgccgttctc aagcagcgtc gcggccatgc tgccgataat gtccgggtgc cgggcgatcg cgagatagtc gttgctcgac aggtgcagcg catcgggccc cgcgatacgg ccgcgcatga tatgcccccc gccccatgtt tcctgcacac gctcacggta ataccgctcg acgcgcgatg tgacgaaagc cggcaacgcc ggccggctat gcctctgttt agtccgatca aagacgcttt tcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMK SVFDRTKQRH SRPALPAFVT SRVERYYRER VQETWGGGHI MRGRIAGPDA LHLSSNDYLA IARHPDIIGS MAATLLENGN GLLMSGIFLH GDCPQLQFEN RLATFMQAEA GVLCQSGYAA NTGLIQSIAS EQTPVYIDMM AHMSLWEGVR SAGAVPVAFR HNDAAHLEQQ IGRYGQGIVL VDSVYSTTGS VCRLVSLADV CERHACVFVV DESHSLGTHG PRGAGLVVEL GLQSRVAFRT ASLAKAFAGR AGFISCPVDF QEYFKFESNP AIFSSTLLPH EISGLDATLS VIECADRRRT RLASSAAWLR QRLTELGYNL NGSRSQIIAL EAGTEQRTIV LRDALESRGI FGSVFCAPAT ARNRALIRLS IHAALSDAQV EKIASVCRDI RSEVELDKWP STRRLGDELS ARRRQRETAS EETAVTA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa gcgtctttga tcggactaaa cagaggcata gccggccggc gttgccggct ttcgtcacat cgcgcgtcga gcggtattac cgtgagcgtg tgcaggaaac atggggcggg gggcatatca tgcgcggccg tatcgcgggg cccgatgcgc tgcacctgtc gagcaacgac tatctcgcga tcgcccggca cccggacatt atcggcagca tggccgcgac gctgcttgag aacggcaacg ggttgttgat gtcgggcatt ttcctgcacg gcgattgccc tcaactccag ttcgaaaacc ggcttgcgac gttcatgcag gcggaggcgg gcgtgctgtg ccagtcgggc tacgcggcca ataccgggct gatccagtcc atcgcgtcgg agcagacgcc ggtctatatc gacatgatgg cgcacatgtc gttgtgggaa ggcgttcgca gtgcgggcgc ggtgccggtc gcgttccggc ataacgacgc cgcgcatctc gagcagcaga tcggacgcta tgggcaaggc atcgtgctgg tggactcggt gtacagcacg actggcagcg tatgccggct cgtctcgctc gcggacgtat gcgaacggca cgcgtgcgtg ttcgtcgtcg acgagtcgca ttcgctcggc acgcacggtc cgcgtggcgc agggctcgtc gtcgaactcg gactgcagtc ccgtgtcgcg tttcgcaccg ccagtctcgc gaaggcgttt gccggcagag ctggtttcat tagctgtccg gtcgatttcc aggagtactt caagttcgaa tcgaatccgg ccattttcag ttccacgcta ttgccgcatg aaatcagcgg gctcgatgcg acactgtcgg tgatcgaatg cgccgaccgg cggcgcacga gactcgcgtc gagcgccgca tggctgaggc agcggttgac ggaactcgga tacaacctga acggcagcag gagtcagatc atcgccctcg aagccggtac ggagcagcgc acgatcgtgt tgcgcgacgc attggagtcg cgcggcattt tcggctcggt gttctgtgcg cccgcgacgg cacgaaatcg ggcgctgatc cgcctgtcga tccacgcggc gctgagcgac gcccaggtcg aaaagattgc cagcgtctgc cgtgacatcc gtagcgaggt cgaactcgac aagtggccgt ccacgcgacg tctcggcgat gaattgtcgg cgcgcagacg ccaacgcgag accgccagcg aagagaccgc tgtgacggct tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BuxeA.00096.a.B1.PS37855
PURIFICATION DATe: 3/2/2016
CONCENTRATION: 46.5mg/ml
OBSERVED MW: 43kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK SVFDRTKQRH SRPALPAFVT SRVERYYRER VQETWGGGHI MRGRIAGPDA LHLSSNDYLA IARHPDIIGS MAATLLENGN GLLMSGIFLH GDCPQLQFEN RLATFMQAEA GVLCQSGYAA NTGLIQSIAS EQTPVYIDMM AHMSLWEGVR SAGAVPVAFR HNDAAHLEQQ IGRYGQGIVL VDSVYSTTGS VCRLVSLADV CERHACVFVV DESHSLGTHG PRGAGLVVEL GLQSRVAFRT ASLAKAFAGR AGFISCPVDF QEYFKFESNP AIFSSTLLPH EISGLDATLS VIECADRRRT RLASSAAWLR QRLTELGYNL NGSRSQIIAL EAGTEQRTIV LRDALESRGI FGSVFCAPAT ARNRALIRLS IHAALSDAQV EKIASVCRDI RSEVELDKWP STRRLGDELS ARRRQRETA
Validated NT Sequence
tggcggtctc gcgttggcgt ctgcgcgccg acaattcatc gccgagacgt cgcgtggacg gccacttgtc gagttcgacc tcgctacgga tgtcacggca gacgctggca atcttttcga cctgggcgtc gctcagcgcc gcgtggatcg acaggcggat cagcgcccga tttcgtgccg tcgcgggcgc acagaacacc gagccgaaaa tgccgcgcga ctccaatgcg tcgcgcaaca cgatcgtgcg ctgctccgta ccggcttcga gggcgatgat ctgactcctg ctgccgttca ggttgtatcc gagttccgtc aaccgctgcc tcagccatgc ggcgctcgac gcgagtctcg tgcgccgccg gtcggcgcat tcgatcaccg acagtgtcgc atcgagcccg ctgatttcat gcggcaatag cgtggaactg aaaatggccg gattcgattc gaacttgaag tactcctgga aatcgaccgg acagctaatg aaaccagctc tgccggcaaa cgccttcgcg agactggcgg tgcgaaacgc gacacgggac tgcagtccga gttcgacgac gagccctgcg ccacgcggac cgtgcgtgcc gagcgaatgc gactcgtcga cgacgaacac gcacgcgtgc cgttcgcata cgtccgcgag cgagacgagc cggcatacgc tgccagtcgt gctgtacacc gagtccacca gcacgatgcc ttgcccatag cgtccgatct gctgctcgag atgcgcggcg tcgttatgcc ggaacgcgac cggcaccgcg cccgcactgc gaacgccttc ccacaacgac atgtgcgcca tcatgtcgat atagaccggc gtctgctccg acgcgatgga ctggatcagc ccggtattgg ccgcgtagcc cgactggcac agcacgcccg cctccgcctg catgaacgtc gcaagccggt tttcgaactg gagttgaggg caatcgccgt gcaggaaaat gcccgacatc aacaacccgt tgccgttctc aagcagcgtc gcggccatgc tgccgataat gtccgggtgc cgggcgatcg cgagatagtc gttgctcgac aggtgcagcg catcgggccc cgcgatacgg ccgcgcatga tatgcccccc gccccatgtt tcctgcacac gctcacggta ataccgctcg acgcgcgatg tgacgaaagc cggcaacgcc ggccggctat gcctctgttt agtccgatca aagacgcttt tcatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHMK SVFDRTKQRH SRPALPAFVT SRVERYYRER VQETWGGGHI MRGRIAGPDA LHLSSNDYLA IARHPDIIGS MAATLLENGN GLLMSGIFLH GDCPQLQFEN RLATFMQAEA GVLCQSGYAA NTGLIQSIAS EQTPVYIDMM AHMSLWEGVR SAGAVPVAFR HNDAAHLEQQ IGRYGQGIVL VDSVYSTTGS VCRLVSLADV CERHACVFVV DESHSLGTHG PRGAGLVVEL GLQSRVAFRT ASLAKAFAGR AGFISCPVDF QEYFKFESNP AIFSSTLLPH EISGLDATLS VIECADRRRT RLASSAAWLR QRLTELGYNL NGSRSQIIAL EAGTEQRTIV LRDALESRGI FGSVFCAPAT ARNRALIRLS IHAALSDAQV EKIASVCRDI RSEVELDKWP STRRLGDELS ARRRQRETAS EETAVTA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAA GCGTCTTTGA TCGGACTAAA CAGAGGCATA GCCGGCCGGC GTTGCCGGCT TTCGTCACAT CGCGCGTCGA GCGGTATTAC CGTGAGCGTG TGCAGGAAAC ATGGGGCGGG GGGCATATCA TGCGCGGCCG TATCGCGGGG CCCGATGCGC TGCACCTGTC GAGCAACGAC TATCTCGCGA TCGCCCGGCA CCCGGACATT ATCGGCAGCA TGGCCGCGAC GCTGCTTGAG AACGGCAACG GGTTGTTGAT GTCGGGCATT TTCCTGCACG GCGATTGCCC TCAACTCCAG TTCGAAAACC GGCTTGCGAC GTTCATGCAG GCGGAGGCGG GCGTGCTGTG CCAGTCGGGC TACGCGGCCA ATACCGGGCT GATCCAGTCC ATCGCGTCGG AGCAGACGCC GGTCTATATC GACATGATGG CGCACATGTC GTTGTGGGAA GGCGTTCGCA GTGCGGGCGC GGTGCCGGTC GCGTTCCGGC ATAACGACGC CGCGCATCTC GAGCAGCAGA TCGGACGCTA TGGGCAAGGC ATCGTGCTGG TGGACTCGGT GTACAGCACG ACTGGCAGCG TATGCCGGCT CGTCTCGCTC GCGGACGTAT GCGAACGGCA CGCGTGCGTG TTCGTCGTCG ACGAGTCGCA TTCGCTCGGC ACGCACGGTC CGCGTGGCGC AGGGCTCGTC GTCGAACTCG GACTGCAGTC CCGTGTCGCG TTTCGCACCG CCAGTCTCGC GAAGGCGTTT GCCGGCAGAG CTGGTTTCAT TAGCTGTCCG GTCGATTTCC AGGAGTACTT CAAGTTCGAA TCGAATCCGG CCATTTTCAG TTCCACGCTA TTGCCGCATG AAATCAGCGG GCTCGATGCG ACACTGTCGG TGATCGAATG CGCCGACCGG CGGCGCACGA GACTCGCGTC GAGCGCCGCA TGGCTGAGGC AGCGGTTGAC GGAACTCGGA TACAACCTGA ACGGCAGCAG GAGTCAGATC ATCGCCCTCG AAGCCGGTAC GGAGCAGCGC ACGATCGTGT TGCGCGACGC ATTGGAGTCG CGCGGCATTT TCGGCTCGGT GTTCTGTGCG CCCGCGACGG CACGAAATCG GGCGCTGATC CGCCTGTCGA TCCACGCGGC GCTGAGCGAC GCCCAGGTCG AAAAGATTGC CAGCGTCTGC CGTGACATCC GTAGCGAGGT CGAACTCGAC AAGTGGCCGT CCACGCGACG TCTCGGCGAT GAATTGTCGG CGCGCAGACG CCAACGCGAG ACCGCCAGCG AAGAGACCGC TGTGACGGCT TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT