SSGCID: Target Page

BuxeA.00095.a

Putative peroxidase

CENTER ID:	BuxeA.00095.a
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00095.a.B1.GE39440	Full length( BuxeA.00095.a )		1	281

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.7258
RefSeq:	YP_554763.1
UniProt:	Q13KH6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPRSGDHGDG TVHPQAHDDN LERFEAHGAA PLPVPNEEGY VEHEGARIWY ASYGAGVPVI LLHGGLGHSG NWGYQLPALL GAGRRVVVVD SRGHGRSTRD ARPYRYELMA SDVLAVMDTL QLERAALVGW SDGACVAMVL GITAAERVAG VFFFGCNMDP SGTKTFAPTP TVERCFSRHA KDYAQLSATP DDFEAFVGAV STMMKTEPNY CADDLARVRV PVAIVHSEHD EFIEPEHADY LARTIPGAEL ILLSGVSHFA PLQRPAQFNQ VMLDFLRRLG G

NT Sequence

TTGCCACGCA GCGGAGACCA CGGCGATGGA ACCGTCCACC CCCAGGCGCA CGACGACAAC CTCGAACGTT TCGAAGCACA CGGCGCGGCG CCGCTGCCGG TCCCGAACGA AGAAGGTTAC GTCGAGCATG AAGGCGCGCG AATCTGGTAC GCGTCGTACG GCGCCGGCGT GCCGGTGATT CTGCTGCACG GCGGCCTCGG GCACAGCGGC AATTGGGGCT ACCAGTTGCC GGCGCTGCTC GGCGCGGGGC GTCGCGTGGT GGTTGTCGAC AGTCGCGGCC ACGGCCGCAG CACCCGCGAT GCGCGGCCCT ACCGCTACGA GCTGATGGCG TCCGACGTGC TGGCCGTGAT GGACACGTTG CAACTCGAAC GCGCCGCGCT GGTGGGCTGG AGCGACGGCG CCTGCGTCGC GATGGTATTG GGCATCACCG CCGCGGAGCG GGTGGCCGGT GTGTTCTTCT TCGGCTGCAA CATGGATCCG AGCGGCACGA AGACGTTCGC GCCGACGCCG ACGGTGGAGC GCTGTTTCAG CCGCCATGCA AAAGACTACG CGCAGCTATC GGCAACGCCG GACGACTTCG AAGCGTTTGT CGGCGCCGTC AGCACGATGA TGAAAACCGA GCCCAACTAT TGCGCCGACG ATCTGGCGCG AGTCCGCGTG CCGGTCGCCA TCGTGCATAG CGAGCATGAC GAATTCATCG AGCCGGAACA CGCCGATTAT CTCGCTCGCA CCATTCCGGG CGCGGAACTG ATTCTGCTGT CCGGCGTGAG CCATTTCGCG CCGCTGCAAC GGCCGGCGCA ATTCAATCAG GTGATGCTCG ACTTCCTGCG CCGGCTTGGC GGC

Details for BuxeA.00095.a.B1.GE39440

HARVESTED ON:	8/5/2015
SEQUENCED ON:	8/13/2015
EXPECTED MW:	32kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML PRSGDHGDGT VHPQAHDDNL ERFEAHGAAP LPVPNEEGYV EHEGARIWYA SYGAGVPVIL LHGGLGHSGN WGYQLPALLG AGRRVVVVDS RGHGRSTRDA RPYRYELMAS DVLAVMDTLQ LERAALVGWS DGACVAMVLG ITAAERVAGV FFFGCNMDPS GTKTFAPTPT VERCFSRHAK DYAQLSATPD DFEAFVGAVS TMMKTEPNYC ADDLARVRVP VAIVHSEHDE FIEPEHADYL ARTIPGAELI LLSGVSHFAP LQRPAQFNQV MLXFXRRLGG

Validated NT Sequence

atcttactca cttagccgcc aagccggcgc angaagtnga gcatcacctg attgaattgc gccggccgtt gcagcggcgc gaaatggctc acgccggaca gcagaatcag ttccgcgccc ggaatggtgc gagcgagata atcggcgtgt tccggctcga tgaattcgtc atgctcgcta tgcacgatgg cgaccggcac gcggactcgc gccagatcgt cggcgcaata gttgggctcg gttttcatca tcgtgctgac ggcgccgaca aacgcttcga agtcgtccgg cgttgccgat agctgcgcgt agtcttttgc atggcggctg aaacagcgct ccaccgtcgg cgtcggcgcg aacgtcttcg tgccgctcgg atccatgttg cagccgaaga agaacacacc ggccacccgc tccgcggcgg tgatgcccaa taccatcgcg acgcaggcgc cgtcgctcca gcccaccagc gcggcgcgtt cgagttgcaa cgtgtccatc acggccagca cgtcggacgc catcagctcg tagcggtagg gccgcgcatc gcgggtgctg cggccgtggc cgcgactgtc gacaaccacc acgcgacgcc ccgcgccgag cagcgccggc aactggtagc cccaattgcc gctgtgcccg aggccgccgt gcagcagaat caccggcacg ccggcgccgt acgacgcgta ccagattcgc gcgccttcat gctcgacgta accttcttcg ttcgggaccg gcagcggcgc cgcgccgtgt gcttcgaaac gttcgaggtt gtcgtcgtgc gcctgggggt ggacggttcc atcgccgtgg tctccgctgc gtggcaacat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

MAHHHHHHMP RSGDHGDGTV HPQAHDDNLE RFEAHGAAPL PVPNEEGYVE HEGARIWYAS YGAGVPVILL HGGLGHSGNW GYQLPALLGA GRRVVVVDSR GHGRSTRDAR PYRYELMASD VLAVMDTLQL ERAALVGWSD GACVAMVLGI TAAERVAGVF FFGCNMDPSG TKTFAPTPTV ERCFSRHAKD YAQLSATPDD FEAFVGAVST MMKTEPNYCA DDLARVRVPV AIVHSEHDEF IEPEHADYLA RTIPGAELIL LSGVSHFAPL QRPAQFNQVM LDFLRRLGG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgccac gcagcggaga ccacggcgat ggaaccgtcc acccccaggc gcacgacgac aacctcgaac gtttcgaagc acacggcgcg gcgccgctgc cggtcccgaa cgaagaaggt tacgtcgagc atgaaggcgc gcgaatctgg tacgcgtcgt acggcgccgg cgtgccggtg attctgctgc acggcggcct cgggcacagc ggcaattggg gctaccagtt gccggcgctg ctcggcgcgg ggcgtcgcgt ggtggttgtc gacagtcgcg gccacggccg cagcacccgc gatgcgcggc cctaccgcta cgagctgatg gcgtccgacg tgctggccgt gatggacacg ttgcaactcg aacgcgccgc gctggtgggc tggagcgacg gcgcctgcgt cgcgatggta ttgggcatca ccgccgcgga gcgggtggcc ggtgtgttct tcttcggctg caacatggat ccgagcggca cgaagacgtt cgcgccgacg ccgacggtgg agcgctgttt cagccgccat gcaaaagact acgcgcagct atcggcaacg ccggacgact tcgaagcgtt tgtcggcgcc gtcagcacga tgatgaaaac cgagcccaac tattgcgccg acgatctggc gcgagtccgc gtgccggtcg ccatcgtgca tagcgagcat gacgaattca tcgagccgga acacgccgat tatctcgctc gcaccattcc gggcgcggaa ctgattctgc tgtccggcgt gagccatttc gcgccgctgc aacggccggc gcaattcaat caggtgatgc tcgacttcct gcgccggctt ggcggctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t