SSGCID: Target Page

BuxeA.00090.b

3-deoxy-D-arabinoheptulosonate-7-phosphate synthase

CENTER ID:	BuxeA.00090.b
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00090.b.B1.GE39499	Full length( BuxeA.00090.b )		1	357

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.517
RefSeq:	YP_557078.1
UniProt:	Q145G3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPPHNTDDVR IRELKELTPP AHLIREFACD ETVSDVIYNS RTSMHRILHG MEDRLIVIIG PCSIHDTKAA MEYAGRLVEQ RKRFAGELEI VMRVYFEKPR TTVGWKGLIN DPYMDNSFKI NEGLRTAREL LLRINELGLP AGTEYLDMIS PQYIADLISW GAIGARTTES QVHRELASGL SCPVGFKNGT DGNVKIAVDA IKAASQPHHF LSVTKGGHSA IVSTAGNEDC HIILRGGKTP NYDADSVNAA CADIGKAGLA ARLMIDASHA NSSKKHENQI PVCADIGRQI ASGDERIVGV MVESNLVAGR QDLQEGCALT YGQSITDACI GWDESVAVLE GLADAVKQRR VARGSGN

NT Sequence

ATGCCCCCGC ACAACACCGA CGATGTCCGC ATCCGCGAAT TGAAAGAACT CACGCCGCCC GCTCACCTGA TCCGTGAATT CGCCTGCGAC GAAACGGTGT CCGACGTGAT CTATAACTCG CGCACCTCGA TGCATCGCAT TCTGCACGGC ATGGAAGATC GCCTGATCGT GATCATCGGA CCGTGCTCGA TTCACGATAC GAAGGCGGCG ATGGAATACG CCGGCCGTCT GGTCGAGCAG CGCAAGCGCT TTGCCGGCGA GCTCGAAATC GTGATGCGCG TGTACTTCGA AAAGCCGCGC ACAACTGTTG GCTGGAAGGG TCTCATCAAC GACCCGTACA TGGACAATAG CTTCAAGATC AACGAAGGCC TGCGCACCGC GCGCGAGTTG CTGTTGCGCA TCAACGAACT CGGCCTGCCG GCCGGCACCG AATACCTCGA CATGATCAGT CCGCAGTACA TCGCCGACCT GATCTCGTGG GGCGCAATCG GCGCGCGGAC CACCGAATCG CAGGTGCACC GCGAACTGGC TTCGGGCCTG TCGTGCCCGG TCGGCTTCAA GAACGGCACG GACGGCAACG TCAAGATCGC GGTCGACGCG ATCAAGGCCG CCTCGCAGCC GCACCATTTC CTGTCGGTCA CGAAGGGCGG CCACTCGGCA ATCGTGTCGA CCGCGGGCAA TGAGGACTGC CACATCATCC TGCGCGGCGG CAAGACGCCG AACTACGACG CGGACAGCGT CAACGCCGCG TGCGCGGACA TCGGCAAGGC CGGTCTCGCC GCGCGCCTGA TGATCGACGC GAGCCACGCG AACAGCTCGA AGAAGCACGA GAATCAGATT CCGGTGTGCG CGGATATCGG CCGCCAGATT GCTTCGGGTG ACGAACGGAT TGTCGGCGTG ATGGTGGAAT CGAATCTGGT GGCGGGCCGC CAGGATCTGC AGGAAGGCTG CGCGCTGACG TACGGCCAGA GCATCACCGA CGCGTGCATC GGCTGGGACG AAAGTGTCGC CGTGCTGGAA GGCCTCGCCG ACGCGGTGAA GCAGCGCCGT GTGGCACGCG GCAGCGGCAA C

Details for BuxeA.00090.b.B1.GE39499

HARVESTED ON:	9/10/2015
SEQUENCED ON:	9/16/2015
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMP PHNTDDVRIR ELKELTPPAH LIREFACDET VSDVIYNSRT SMHRILHGME DRLIVIIGPC SIHDTKAAME YAGRLVEQRK RFAGELEIVM RVYFEKPRTT VGWKGLINDP YMDNSFKINE GLRTARELLL RINELGLPAG TEYLDMISPQ YIADLISWGA IGARTTESQV HRELASGLSC PVGFKNGTDG NVKIAVDAIK AASQPHHFLS VTKGGHSAIV STAGNEDCHI ILRGGKTPNY DADSVNAACA DIGKAGLAAR LMIDASHANS SKKHENQIPV CADIGRQIAS GDERIVGVMV ESNLVAGRQD LQEGCALTYG QSITDACIGW DESVAVLEGL ADAVKQR

Validated NT Sequence

ggcgctgctt caccgcgtcg gcgaggcctt ccagcacggc gacactttcg tcccagccga tgcacgcgtc ggtgatgctc tggccgtacg tcagcgcgca gccttcctgc agatcctggc ggcccgccac cagattcgat tccaccatca cgccgacaat ccgttcgtca cccgaagcaa tctggcggcc gatatccgcg cacaccggaa tctgattctc gtgcttcttc gagctgttcg cgtggctcgc gtcgatcatc aggcgcgcgg cgagaccggc cttgccgatg tccgcgcacg cggcgttgac gctgtccgcg tcgtagttcg gcgtcttgcc gccgcgcagg atgatgtggc agtcctcatt gcccgcggtc gacacgattg ccgagtggcc gcccttcgtg accgacagga aatggtgcgg ctgcgaggcg gccttgatcg cgtcgaccgc gatcttgacg ttgccgtccg tgccgttctt gaagccgacc gggcacgaca ggcccgaagc cagttcgcgg tgcacctgcg attcggtggt ccgcgcgccg attgcgcccc acgagatcag gtcggcgatg tactgcggac tgatcatgtc gaggtattcg gtgccggccg gcaggccgag ttcgttgatg cgcaacagca actcgcgcgc ggtgcgcagg ccttcgttga tcttgaagct attgtccatg tacgggtcgt tgatgagacc cttccagcca acagttgtgc gcggcttttc gaagtacacg cgcatcacga tttcgagctc gccggcaaag cgcttgcgct gctcgaccag acggccggcg tattccatcg ccgccttcgt atcgtgaatc gagcacggtc cgatgatcac gatcaggcga tcttccatgc cgtgcagaat gcgatgcatc gaggtgcgcg agttatagat cacgtcggac accgtttcgt cgcaggcgaa ttcacggatc aggtgagcgg gcggcgtgag ttctttcaat tcgcggatgc ggacatcgtc ggtgttgtgc gggggcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccc cgcacaacac cgacgatgtc cgcatccgcg aattgaaaga actcacgccg cccgctcacc tgatccgtga attcgcctgc gacgaaacgg tgtccgacgt gatctataac tcgcgcacct cgatgcatcg cattctgcac ggcatggaag atcgcctgat cgtgatcatc ggaccgtgct cgattcacga tacgaaggcg gcgatggaat acgccggccg tctggtcgag cagcgcaagc gctttgccgg cgagctcgaa atcgtgatgc gcgtgtactt cgaaaagccg cgcacaactg ttggctggaa gggtctcatc aacgacccgt acatggacaa tagcttcaag atcaacgaag gcctgcgcac cgcgcgcgag ttgctgttgc gcatcaacga actcggcctg ccggccggca ccgaatacct cgacatgatc agtccgcagt acatcgccga cctgatctcg tggggcgcaa tcggcgcgcg gaccaccgaa tcgcaggtgc accgcgaact ggcttcgggc ctgtcgtgcc cggtcggctt caagaacggc acggacggca acgtcaagat cgcggtcgac gcgatcaagg ccgcctcgca gccgcaccat ttcctgtcgg tcacgaaggg cggccactcg gcaatcgtgt cgaccgcggg caatgaggac tgccacatca tcctgcgcgg cggcaagacg ccgaactacg acgcggacag cgtcaacgcc gcgtgcgcgg acatcggcaa ggccggtctc gccgcgcgcc tgatgatcga cgcgagccac gcgaacagct cgaagaagca cgagaatcag attccggtgt gcgcggatat cggccgccag attgcttcgg gtgacgaacg gattgtcggc gtgatggtgg aatcgaatct ggtggcgggc cgccaggatc tgcaggaagg ctgcgcgctg acgtacggcc agagcatcac cgacgcgtgc atcggctggg acgaaagtgt cgccgtgctg gaaggcctcg ccgacgcggt gaagcagcgc cgtgtggcac gcggcagcgg caactgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat