BuxeA.00090.a

3-deoxy-D-arabinoheptulosonate-7-phosphate synthase

CENTER ID: BuxeA.00090.a
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00090.a.B1.GE39498 Full length( BuxeA.00090.a ) 1 372
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00090.a.B1.PS37856 Full length( BuxeA.00090.a ) 1 372
BuxeA.00090.a.B1.PS37857 Full length( BuxeA.00090.a ) 1 372

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.6811
RefSeq: YP_554347.1
UniProt: Q13LP2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNAIDNPLHD QEVGSADATQ DTTRIDDVRI GAVRPLISPA LLQDELPTPP SVQTLVEKTR AEIADILHGR DDRLVMIVGP CSIHDHDQAI DYARKLKVAA DTYRDDLLIV MRVYFEKPRT TVGWKGYIND PRLDGSFRIN EGLRLARQLL LDINGLGLST ATEFLDLLSP QYIADLIAWG AIGARTTESQ SHRQLASGLS CPIGFKNGTD GGVQIAADAI VAARASHAFM GMTKMGMAAI FETRGNDDAH VILRGGKKGP NYDSASVDAT CVALKSAGLR EQVMIDCSHA NSGKSHLRQL EVVQDIALQL SQRERRIIGV MLESHLEEGR QDLKPGVPLR HGVSITDACV SWAQTEPVLE TLAEATRKRR AG
NT Sequence
TTGAACGCCA TCGACAATCC TTTGCACGAT CAGGAAGTCG GCTCGGCCGA CGCCACCCAG GACACCACGC GCATCGACGA CGTTCGGATC GGCGCGGTAC GTCCGCTGAT TTCCCCTGCG CTGTTGCAGG ACGAGTTGCC CACACCGCCT TCGGTGCAGA CGCTGGTCGA GAAGACCCGC GCGGAAATCG CCGACATTCT GCATGGCCGC GACGACCGGC TCGTGATGAT CGTCGGACCG TGCTCGATTC ACGACCACGA CCAGGCGATC GACTATGCGC GCAAGCTGAA AGTCGCCGCC GACACTTACA GGGACGACCT GCTGATCGTA ATGCGGGTCT ACTTCGAGAA GCCGCGCACT ACCGTCGGCT GGAAAGGCTA TATCAACGAC CCGCGGCTCG ACGGCAGTTT CCGTATCAAT GAAGGTCTGC GTCTCGCTCG GCAATTGCTG CTCGATATCA ACGGTCTCGG CCTGTCCACC GCCACCGAGT TTCTCGACCT GCTGAGCCCG CAGTACATCG CCGATCTGAT CGCGTGGGGC GCTATCGGCG CGCGCACGAC GGAGAGTCAG AGCCATCGTC AACTCGCTTC GGGACTGAGC TGCCCGATCG GCTTCAAGAA CGGCACGGAC GGCGGCGTGC AGATCGCCGC GGACGCGATC GTCGCCGCGC GCGCGAGCCA TGCGTTCATG GGCATGACGA AAATGGGCAT GGCCGCGATC TTCGAAACGC GCGGCAACGA CGACGCGCAC GTGATCCTGC GCGGCGGCAA GAAAGGGCCT AACTACGACA GCGCGTCGGT GGACGCGACT TGCGTCGCGC TGAAATCAGC GGGACTGCGC GAGCAGGTCA TGATCGACTG TTCGCATGCG AACTCGGGCA AATCGCATTT GCGTCAACTC GAAGTGGTGC AGGATATCGC GCTGCAGTTG TCGCAGCGCG AGCGCCGCAT TATCGGCGTC ATGCTGGAAA GTCATCTGGA AGAAGGCCGC CAGGATCTGA AGCCTGGTGT GCCGTTGCGT CACGGCGTAT CGATCACGGA TGCCTGCGTC AGCTGGGCGC AGACCGAGCC GGTGCTCGAA ACACTCGCCG AGGCCACCCG CAAACGCCGC GCGGGC
Details for BuxeA.00090.a.B1.GE39498
HARVESTED ON: 9/10/2015
SEQUENCED ON: 9/16/2015
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHML NAIDNPLHDQ EVGSADATQD TTRIDDVRIG AVRPLISPAL LQDELPTPPS VQTLVEKTRA EIADILHGRD DRLVMIVGPC SIHDHDQAID YARKLKVAAD TYRDDLLIVM RVYFEKPRTT VGWKGYINDP RLDGSFRINE GLRLARQLLL DINGLGLSTA TEFLDLLSPQ YIADLIAWGA IGARTTESQS HRQLASGLSC PIGFKNGTDG GVQIAADAIV AARASHAFMG MTKMGMAAIF ETRGNDDAHV ILRGGKKGPN YDSASVDATC VALKSAGLRE QVMIDCSHAN SGKSHLRQLE VVQDIALQLS QRERRIIGVM LESHLEEGRQ DLKPGVPLRH GVSITDACVS WAQTEPVLET LA
Validated NT Sequence
ctcggcgagt gtttcgagca ccggctcggt ctgcgcccag ctgacgcagg catccgtgat cgatacgccg tgacgcaacg gcacaccagg cttcagatcc tggcggcctt cttccagatg actttccagc atgacgccga taatgcggcg ctcgcgctgc gacaactgca gcgcgatatc ctgcaccact tcgagttgac gcaaatgcga tttgcccgag ttcgcatgcg aacagtcgat catgacctgc tcgcgcagtc ccgctgattt cagcgcgacg caagtcgcgt ccaccgacgc gctgtcgtag ttaggccctt tcttgccgcc gcgcaggatc acgtgcgcgt cgtcgttgcc gcgcgtttcg aagatcgcgg ccatgcccat tttcgtcatg cccatgaacg catggctcgc gcgcgcggcg acgatcgcgt ccgcggcgat ctgcacgccg ccgtccgtgc cgttcttgaa gccgatcggg cagctcagtc ccgaagcgag ttgacgatgg ctctgactct ccgtcgtgcg cgcgccgata gcgccccacg cgatcagatc ggcgatgtac tgcgggctca gcaggtcgag aaactcggtg gcggtggaca ggccgagacc gttgatatcg agcagcaatt gccgagcgag acgcagacct tcattgatac ggaaactgcc gtcgagccgc gggtcgttga tatagccttt ccagccgacg gtagtgcgcg gcttctcgaa gtagacccgc attacgatca gcaggtcgtc cctgtaagtg tcggcggcga ctttcagctt gcgcgcatag tcgatcgcct ggtcgtggtc gtgaatcgag cacggtccga cgatcatcac gagccggtcg tcgcggccat gcagaatgtc ggcgatttcc gcgcgggtct tctcgaccag cgtctgcacc gaaggcggtg tgggcaactc gtcctgcaac agcgcagggg aaatcagcgg acgtaccgcg ccgatccgaa cgtcgtcgat gcgcgtggtg tcctgggtgg cgtcggccga gccgacttcc tgatcgtgca aaggattgtc gatggcgttc aacatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMN AIDNPLHDQE VGSADATQDT TRIDDVRIGA VRPLISPALL QDELPTPPSV QTLVEKTRAE IADILHGRDD RLVMIVGPCS IHDHDQAIDY ARKLKVAADT YRDDLLIVMR VYFEKPRTTV GWKGYINDPR LDGSFRINEG LRLARQLLLD INGLGLSTAT EFLDLLSPQY IADLIAWGAI GARTTESQSH RQLASGLSCP IGFKNGTDGG VQIAADAIVA ARASHAFMGM TKMGMAAIFE TRGNDDAHVI LRGGKKGPNY DSASVDATCV ALKSAGLREQ VMIDCSHANS GKSHLRQLEV VQDIALQLSQ RERRIIGVML ESHLEEGRQD LKPGVPLRHG VSITDACVSW AQTEPVLETL AEATRKRRAG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgaacg ccatcgacaa tcctttgcac gatcaggaag tcggctcggc cgacgccacc caggacacca cgcgcatcga cgacgttcgg atcggcgcgg tacgtccgct gatttcccct gcgctgttgc aggacgagtt gcccacaccg ccttcggtgc agacgctggt cgagaagacc cgcgcggaaa tcgccgacat tctgcatggc cgcgacgacc ggctcgtgat gatcgtcgga ccgtgctcga ttcacgacca cgaccaggcg atcgactatg cgcgcaagct gaaagtcgcc gccgacactt acagggacga cctgctgatc gtaatgcggg tctacttcga gaagccgcgc actaccgtcg gctggaaagg ctatatcaac gacccgcggc tcgacggcag tttccgtatc aatgaaggtc tgcgtctcgc tcggcaattg ctgctcgata tcaacggtct cggcctgtcc accgccaccg agtttctcga cctgctgagc ccgcagtaca tcgccgatct gatcgcgtgg ggcgctatcg gcgcgcgcac gacggagagt cagagccatc gtcaactcgc ttcgggactg agctgcccga tcggcttcaa gaacggcacg gacggcggcg tgcagatcgc cgcggacgcg atcgtcgccg cgcgcgcgag ccatgcgttc atgggcatga cgaaaatggg catggccgcg atcttcgaaa cgcgcggcaa cgacgacgcg cacgtgatcc tgcgcggcgg caagaaaggg cctaactacg acagcgcgtc ggtggacgcg acttgcgtcg cgctgaaatc agcgggactg cgcgagcagg tcatgatcga ctgttcgcat gcgaactcgg gcaaatcgca tttgcgtcaa ctcgaagtgg tgcaggatat cgcgctgcag ttgtcgcagc gcgagcgccg cattatcggc gtcatgctgg aaagtcatct ggaagaaggc cgccaggatc tgaagcctgg tgtgccgttg cgtcacggcg tatcgatcac ggatgcctgc gtcagctggg cgcagaccga gccggtgctc gaaacactcg ccgaggccac ccgcaaacgc cgcgcgggct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BuxeA.00090.a.B1.PS37856
PURIFICATION DATe: 3/3/2016
CONCENTRATION: 59mg/ml
OBSERVED MW: 45kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML NAIDNPLHDQ EVGSADATQD TTRIDDVRIG AVRPLISPAL LQDELPTPPS VQTLVEKTRA EIADILHGRD DRLVMIVGPC SIHDHDQAID YARKLKVAAD TYRDDLLIVM RVYFEKPRTT VGWKGYINDP RLDGSFRINE GLRLARQLLL DINGLGLSTA TEFLDLLSPQ YIADLIAWGA IGARTTESQS HRQLASGLSC PIGFKNGTDG GVQIAADAIV AARASHAFMG MTKMGMAAIF ETRGNDDAHV ILRGGKKGPN YDSASVDATC VALKSAGLRE QVMIDCSHAN SGKSHLRQLE VVQDIALQLS QRERRIIGVM LESHLEEGRQ DLKPGVPLRH GVSITDACVS WAQTEPVLET LA
Validated NT Sequence
ctcggcgagt gtttcgagca ccggctcggt ctgcgcccag ctgacgcagg catccgtgat cgatacgccg tgacgcaacg gcacaccagg cttcagatcc tggcggcctt cttccagatg actttccagc atgacgccga taatgcggcg ctcgcgctgc gacaactgca gcgcgatatc ctgcaccact tcgagttgac gcaaatgcga tttgcccgag ttcgcatgcg aacagtcgat catgacctgc tcgcgcagtc ccgctgattt cagcgcgacg caagtcgcgt ccaccgacgc gctgtcgtag ttaggccctt tcttgccgcc gcgcaggatc acgtgcgcgt cgtcgttgcc gcgcgtttcg aagatcgcgg ccatgcccat tttcgtcatg cccatgaacg catggctcgc gcgcgcggcg acgatcgcgt ccgcggcgat ctgcacgccg ccgtccgtgc cgttcttgaa gccgatcggg cagctcagtc ccgaagcgag ttgacgatgg ctctgactct ccgtcgtgcg cgcgccgata gcgccccacg cgatcagatc ggcgatgtac tgcgggctca gcaggtcgag aaactcggtg gcggtggaca ggccgagacc gttgatatcg agcagcaatt gccgagcgag acgcagacct tcattgatac ggaaactgcc gtcgagccgc gggtcgttga tatagccttt ccagccgacg gtagtgcgcg gcttctcgaa gtagacccgc attacgatca gcaggtcgtc cctgtaagtg tcggcggcga ctttcagctt gcgcgcatag tcgatcgcct ggtcgtggtc gtgaatcgag cacggtccga cgatcatcac gagccggtcg tcgcggccat gcagaatgtc ggcgatttcc gcgcgggtct tctcgaccag cgtctgcacc gaaggcggtg tgggcaactc gtcctgcaac agcgcagggg aaatcagcgg acgtaccgcg ccgatccgaa cgtcgtcgat gcgcgtggtg tcctgggtgg cgtcggccga gccgacttcc tgatcgtgca aaggattgtc gatggcgttc aacatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMN AIDNPLHDQE VGSADATQDT TRIDDVRIGA VRPLISPALL QDELPTPPSV QTLVEKTRAE IADILHGRDD RLVMIVGPCS IHDHDQAIDY ARKLKVAADT YRDDLLIVMR VYFEKPRTTV GWKGYINDPR LDGSFRINEG LRLARQLLLD INGLGLSTAT EFLDLLSPQY IADLIAWGAI GARTTESQSH RQLASGLSCP IGFKNGTDGG VQIAADAIVA ARASHAFMGM TKMGMAAIFE TRGNDDAHVI LRGGKKGPNY DSASVDATCV ALKSAGLREQ VMIDCSHANS GKSHLRQLEV VQDIALQLSQ RERRIIGVML ESHLEEGRQD LKPGVPLRHG VSITDACVSW AQTEPVLETL AEATRKRRAG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTGAACG CCATCGACAA TCCTTTGCAC GATCAGGAAG TCGGCTCGGC CGACGCCACC CAGGACACCA CGCGCATCGA CGACGTTCGG ATCGGCGCGG TACGTCCGCT GATTTCCCCT GCGCTGTTGC AGGACGAGTT GCCCACACCG CCTTCGGTGC AGACGCTGGT CGAGAAGACC CGCGCGGAAA TCGCCGACAT TCTGCATGGC CGCGACGACC GGCTCGTGAT GATCGTCGGA CCGTGCTCGA TTCACGACCA CGACCAGGCG ATCGACTATG CGCGCAAGCT GAAAGTCGCC GCCGACACTT ACAGGGACGA CCTGCTGATC GTAATGCGGG TCTACTTCGA GAAGCCGCGC ACTACCGTCG GCTGGAAAGG CTATATCAAC GACCCGCGGC TCGACGGCAG TTTCCGTATC AATGAAGGTC TGCGTCTCGC TCGGCAATTG CTGCTCGATA TCAACGGTCT CGGCCTGTCC ACCGCCACCG AGTTTCTCGA CCTGCTGAGC CCGCAGTACA TCGCCGATCT GATCGCGTGG GGCGCTATCG GCGCGCGCAC GACGGAGAGT CAGAGCCATC GTCAACTCGC TTCGGGACTG AGCTGCCCGA TCGGCTTCAA GAACGGCACG GACGGCGGCG TGCAGATCGC CGCGGACGCG ATCGTCGCCG CGCGCGCGAG CCATGCGTTC ATGGGCATGA CGAAAATGGG CATGGCCGCG ATCTTCGAAA CGCGCGGCAA CGACGACGCG CACGTGATCC TGCGCGGCGG CAAGAAAGGG CCTAACTACG ACAGCGCGTC GGTGGACGCG ACTTGCGTCG CGCTGAAATC AGCGGGACTG CGCGAGCAGG TCATGATCGA CTGTTCGCAT GCGAACTCGG GCAAATCGCA TTTGCGTCAA CTCGAAGTGG TGCAGGATAT CGCGCTGCAG TTGTCGCAGC GCGAGCGCCG CATTATCGGC GTCATGCTGG AAAGTCATCT GGAAGAAGGC CGCCAGGATC TGAAGCCTGG TGTGCCGTTG CGTCACGGCG TATCGATCAC GGATGCCTGC GTCAGCTGGG CGCAGACCGA GCCGGTGCTC GAAACACTCG CCGAGGCCAC CCGCAAACGC CGCGCGGGCT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT
Details for BuxeA.00090.a.B1.PS37857
PURIFICATION DATe: 3/3/2016
CONCENTRATION: 45.9mg/ml
OBSERVED MW: 45kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML NAIDNPLHDQ EVGSADATQD TTRIDDVRIG AVRPLISPAL LQDELPTPPS VQTLVEKTRA EIADILHGRD DRLVMIVGPC SIHDHDQAID YARKLKVAAD TYRDDLLIVM RVYFEKPRTT VGWKGYINDP RLDGSFRINE GLRLARQLLL DINGLGLSTA TEFLDLLSPQ YIADLIAWGA IGARTTESQS HRQLASGLSC PIGFKNGTDG GVQIAADAIV AARASHAFMG MTKMGMAAIF ETRGNDDAHV ILRGGKKGPN YDSASVDATC VALKSAGLRE QVMIDCSHAN SGKSHLRQLE VVQDIALQLS QRERRIIGVM LESHLEEGRQ DLKPGVPLRH GVSITDACVS WAQTEPVLET LA
Validated NT Sequence
ctcggcgagt gtttcgagca ccggctcggt ctgcgcccag ctgacgcagg catccgtgat cgatacgccg tgacgcaacg gcacaccagg cttcagatcc tggcggcctt cttccagatg actttccagc atgacgccga taatgcggcg ctcgcgctgc gacaactgca gcgcgatatc ctgcaccact tcgagttgac gcaaatgcga tttgcccgag ttcgcatgcg aacagtcgat catgacctgc tcgcgcagtc ccgctgattt cagcgcgacg caagtcgcgt ccaccgacgc gctgtcgtag ttaggccctt tcttgccgcc gcgcaggatc acgtgcgcgt cgtcgttgcc gcgcgtttcg aagatcgcgg ccatgcccat tttcgtcatg cccatgaacg catggctcgc gcgcgcggcg acgatcgcgt ccgcggcgat ctgcacgccg ccgtccgtgc cgttcttgaa gccgatcggg cagctcagtc ccgaagcgag ttgacgatgg ctctgactct ccgtcgtgcg cgcgccgata gcgccccacg cgatcagatc ggcgatgtac tgcgggctca gcaggtcgag aaactcggtg gcggtggaca ggccgagacc gttgatatcg agcagcaatt gccgagcgag acgcagacct tcattgatac ggaaactgcc gtcgagccgc gggtcgttga tatagccttt ccagccgacg gtagtgcgcg gcttctcgaa gtagacccgc attacgatca gcaggtcgtc cctgtaagtg tcggcggcga ctttcagctt gcgcgcatag tcgatcgcct ggtcgtggtc gtgaatcgag cacggtccga cgatcatcac gagccggtcg tcgcggccat gcagaatgtc ggcgatttcc gcgcgggtct tctcgaccag cgtctgcacc gaaggcggtg tgggcaactc gtcctgcaac agcgcagggg aaatcagcgg acgtaccgcg ccgatccgaa cgtcgtcgat gcgcgtggtg tcctgggtgg cgtcggccga gccgacttcc tgatcgtgca aaggattgtc gatggcgttc aacatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMN AIDNPLHDQE VGSADATQDT TRIDDVRIGA VRPLISPALL QDELPTPPSV QTLVEKTRAE IADILHGRDD RLVMIVGPCS IHDHDQAIDY ARKLKVAADT YRDDLLIVMR VYFEKPRTTV GWKGYINDPR LDGSFRINEG LRLARQLLLD INGLGLSTAT EFLDLLSPQY IADLIAWGAI GARTTESQSH RQLASGLSCP IGFKNGTDGG VQIAADAIVA ARASHAFMGM TKMGMAAIFE TRGNDDAHVI LRGGKKGPNY DSASVDATCV ALKSAGLREQ VMIDCSHANS GKSHLRQLEV VQDIALQLSQ RERRIIGVML ESHLEEGRQD LKPGVPLRHG VSITDACVSW AQTEPVLETL AEATRKRRAG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTGAACG CCATCGACAA TCCTTTGCAC GATCAGGAAG TCGGCTCGGC CGACGCCACC CAGGACACCA CGCGCATCGA CGACGTTCGG ATCGGCGCGG TACGTCCGCT GATTTCCCCT GCGCTGTTGC AGGACGAGTT GCCCACACCG CCTTCGGTGC AGACGCTGGT CGAGAAGACC CGCGCGGAAA TCGCCGACAT TCTGCATGGC CGCGACGACC GGCTCGTGAT GATCGTCGGA CCGTGCTCGA TTCACGACCA CGACCAGGCG ATCGACTATG CGCGCAAGCT GAAAGTCGCC GCCGACACTT ACAGGGACGA CCTGCTGATC GTAATGCGGG TCTACTTCGA GAAGCCGCGC ACTACCGTCG GCTGGAAAGG CTATATCAAC GACCCGCGGC TCGACGGCAG TTTCCGTATC AATGAAGGTC TGCGTCTCGC TCGGCAATTG CTGCTCGATA TCAACGGTCT CGGCCTGTCC ACCGCCACCG AGTTTCTCGA CCTGCTGAGC CCGCAGTACA TCGCCGATCT GATCGCGTGG GGCGCTATCG GCGCGCGCAC GACGGAGAGT CAGAGCCATC GTCAACTCGC TTCGGGACTG AGCTGCCCGA TCGGCTTCAA GAACGGCACG GACGGCGGCG TGCAGATCGC CGCGGACGCG ATCGTCGCCG CGCGCGCGAG CCATGCGTTC ATGGGCATGA CGAAAATGGG CATGGCCGCG ATCTTCGAAA CGCGCGGCAA CGACGACGCG CACGTGATCC TGCGCGGCGG CAAGAAAGGG CCTAACTACG ACAGCGCGTC GGTGGACGCG ACTTGCGTCG CGCTGAAATC AGCGGGACTG CGCGAGCAGG TCATGATCGA CTGTTCGCAT GCGAACTCGG GCAAATCGCA TTTGCGTCAA CTCGAAGTGG TGCAGGATAT CGCGCTGCAG TTGTCGCAGC GCGAGCGCCG CATTATCGGC GTCATGCTGG AAAGTCATCT GGAAGAAGGC CGCCAGGATC TGAAGCCTGG TGTGCCGTTG CGTCACGGCG TATCGATCAC GGATGCCTGC GTCAGCTGGG CGCAGACCGA GCCGGTGCTC GAAACACTCG CCGAGGCCAC CCGCAAACGC CGCGCGGGCT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT