BuxeA.00088.a
Ornithine carbamoyltransferase
| CENTER ID: | BuxeA.00088.a |
| ORGANISM: | Burkholderia xenovorans LB400 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuxeA.00088.a.A1.GE32491 | Full length( BuxeA.00088.a ) | 1 | 333 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuxeA.00088.a.A1.PS01326 | Full length( BuxeA.00088.a ) | 1 | 333 | ||
| BuxeA.00088.a.B2.PW39423 | AlphaFold domain | 2 | 332 |
Structures
9Y83
DEPOSITED: 9/11/2025
DETERMINATION: XRay
CLONE: BuxeA.00088.a.B2.GE45648
PROTEIN: BuxeA.00088.a.B2.PW39423
9Y8H
DEPOSITED: 9/11/2025
DETERMINATION: XRay
CLONE: BuxeA.00088.a.B2.GE45648
PROTEIN: BuxeA.00088.a.B2.PW39423
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|266265.5.peg.8507 |
| RefSeq: | YP_555981.1 |
| UniProt: | Q13H08 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFNVHNRSYL TLIEYTPRQI RYLLDLSRDL KRAKYAGTET PRLNGKNIAL IFEKTSTRTR CAFEVAAHDQ GAHVTYIDPN GSQIGHKESM KDTARVLGRM YDAIEYRGFG QEIVEELAKY AGVPVYNGLT DEFHPTQMLA DVLTMHEFSD RPIHDIAYCY IGDAHNNTGN SLMIVGAKLG MDVRLCAPRC LWPHDELIEQ CRAIAATTGA RLTLTEKPEE AVKGVDFIYT DVWVSMGEPF EKWGARIEEL LPYRVNAALL AASGNPRVKF MHCLPAFHDS NTHVGKQIAD QYGLPNGVEV TDDVFESDAS IVFEQAENRL HTIKAILVAT LTG
NT Sequence
ATGTTCAACG TACACAACCG CAGCTACCTC ACGCTGATCG AATATACGCC TCGCCAGATT CGCTACCTGC TCGACCTGTC CCGCGATCTG AAGCGCGCCA AGTATGCCGG AACCGAAACG CCCCGCCTGA ACGGCAAGAA TATCGCGCTG ATCTTTGAGA AGACCTCGAC GCGCACGCGT TGTGCATTCG AAGTGGCGGC GCACGACCAG GGCGCCCATG TCACCTATAT CGACCCGAAC GGCTCGCAGA TCGGCCACAA GGAATCGATG AAGGACACCG CCCGGGTGCT GGGCCGCATG TATGACGCGA TCGAATATCG TGGATTCGGT CAGGAGATCG TCGAAGAGCT TGCGAAGTAT GCCGGCGTGC CTGTCTACAA CGGCTTGACT GACGAGTTCC ACCCGACGCA GATGCTGGCC GACGTGCTAA CGATGCACGA GTTCAGCGAC AGGCCGATCC ACGACATCGC CTATTGCTAC ATAGGCGACG CGCACAACAA TACTGGCAAT TCGCTGATGA TTGTGGGCGC CAAGCTCGGC ATGGATGTCC GCCTGTGTGC TCCGCGCTGC CTGTGGCCGC ACGATGAACT GATCGAACAG TGCCGGGCAA TCGCGGCCAC GACGGGCGCG CGCCTGACGT TGACCGAAAA GCCGGAGGAG GCCGTGAAAG GCGTCGACTT CATCTATACC GACGTATGGG TATCGATGGG CGAGCCGTTC GAGAAGTGGG GCGCGCGGAT CGAGGAGCTT TTGCCGTACC GGGTGAATGC CGCACTGCTG GCTGCAAGTG GTAATCCGCG GGTGAAGTTC ATGCATTGCC TGCCGGCGTT TCATGACTCG AACACCCACG TCGGCAAGCA GATTGCTGAC CAATATGGGC TCCCGAACGG CGTCGAGGTG ACCGACGACG TGTTCGAATC CGACGCTTCG ATCGTCTTCG AGCAGGCGGA GAACCGCCTT CATACGATCA AGGCGATTCT GGTCGCTACG CTCACCGGC
Details for BuxeA.00088.a.A1.GE32491
| HARVESTED ON: | 11/8/2011 |
| SEQUENCED ON: | 11/11/2011 |
| EXPECTED MW: | 39kDa |
| OBSERVED MW: | 39kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MGTLEAQTQG PGSMFNVHNR SYLTLIEYTP RQIRYLLDLS RDLKRAKYAG TETPRLNGKN IALIFEKTST RTRCAFEVAA HDQGAHVTYI DPNGSQIGHK ESMKDTARVL GRMYDAIEYR GFGQEIVEEL AKYAGVPVYN GLTDEFHPTQ MLADVLTMHE FSDRPIHDIA YCYIGDAHNN TGNSLMIVGA KLGMDVRLCA PRCLWPHDEL IEQCRAIAAT TGARLTLTEK PEEAVKGVDF IYTDVWVSMG EPFEKWGARI EELLPYRVNA ALLAASGNPR VKFMHCLPAF HDSNTHVGKQ IADQYGLPNG VEVTDDVFES DASIVFEQAE NRLHTIKAIL VATLTG
Validated NT Sequence
tattagccgg tgagcgtagc gaccagaatc gccttgatcg tatgaaggcg gttctccgcc tgctcgaaga cgatcgaagc gtcggattcg aacacgtcgt cggtcacctc gacgccgttc gggagcccat attggtcagc aatctgcttg ccgacgtggg tgttcgagtc atgaaacgcc ggcaggcaat gcatgaactt cacccgcgga ttaccacttg cagccagcag tgcggcattc acccggtacg gcaaaagctc ctcgatccgc gcgccccact tctcgaacgg ctcgcccatc gatacccata cgtcggtata gatgaagtcg acgcctttca cggcctcctc cggcttttcg gtcaacgtca ggcgcgcgcc cgtcgtggcc gcgattgccc ggcactgttc gatcagttca tcgtgcggcc acaggcagcg cggagcacac aggcggacat ccatgccgag cttggcgccc acaatcatca gcgaattgcc agtattgttg tgcgcgtcgc ctatgtagca ataggcgatg tcgtggatcg gcctgtcgct gaactcgtgc atcgttagca cgtcggccag catctgcgtc gggtggaact cgtcagtcaa gccgttgtag acaggcacgc cggcatactt cgcaagctct tcgacgatct cctgaccgaa tccacgatat tcgatcgcgt catacatgcg gcccagcacc cgggcggtgt ccttcatcga ttccttgtgg ccgatctgcg agccgttcgg gtcgatatag gtgacatggg cgccctggtc gtgcgccgcc acttcgaatg cacaacgcgt gcgcgtcgag gtcttctcaa agatcagcgc gatattcttg ccgttcaggc ggggcgtttc ggttccggca tacttggcgc gcttcagatc gcgggacagg tcgagcaggt agcgaatctg gcgaggcgta tattcgatca gcgtgaggta gctgcggttg tgtacgttga acatcgaacc aggaccctgg gtctgagctt ccagggtacc cata
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFNVHNRSY LTLIEYTPRQ IRYLLDLSRD LKRAKYAGTE TPRLNGKNIA LIFEKTSTRT RCAFEVAAHD QGAHVTYIDP NGSQIGHKES MKDTARVLGR MYDAIEYRGF GQEIVEELAK YAGVPVYNGL TDEFHPTQML ADVLTMHEFS DRPIHDIAYC YIGDAHNNTG NSLMIVGAKL GMDVRLCAPR CLWPHDELIE QCRAIAATTG ARLTLTEKPE EAVKGVDFIY TDVWVSMGEP FEKWGARIEE LLPYRVNAAL LAASGNPRVK FMHCLPAFHD SNTHVGKQIA DQYGLPNGVE VTDDVFESDA SIVFEQAENR LHTIKAILVA TLTG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttcaacgta cacaaccgca gctacctcac gctgatcgaa tatacgcctc gccagattcg ctacctgctc gacctgtccc gcgatctgaa gcgcgccaag tatgccggaa ccgaaacgcc ccgcctgaac ggcaagaata tcgcgctgat ctttgagaag acctcgacgc gcacgcgttg tgcattcgaa gtggcggcgc acgaccaggg cgcccatgtc acctatatcg acccgaacgg ctcgcagatc ggccacaagg aatcgatgaa ggacaccgcc cgggtgctgg gccgcatgta tgacgcgatc gaatatcgtg gattcggtca ggagatcgtc gaagagcttg cgaagtatgc cggcgtgcct gtctacaacg gcttgactga cgagttccac ccgacgcaga tgctggccga cgtgctaacg atgcacgagt tcagcgacag gccgatccac gacatcgcct attgctacat aggcgacgcg cacaacaata ctggcaattc gctgatgatt gtgggcgcca agctcggcat ggatgtccgc ctgtgtgctc cgcgctgcct gtggccgcac gatgaactga tcgaacagtg ccgggcaatc gcggccacga cgggcgcgcg cctgacgttg accgaaaagc cggaggaggc cgtgaaaggc gtcgacttca tctataccga cgtatgggta tcgatgggcg agccgttcga gaagtggggc gcgcggatcg aggagctttt gccgtaccgg gtgaatgccg cactgctggc tgcaagtggt aatccgcggg tgaagttcat gcattgcctg ccggcgtttc atgactcgaa cacccacgtc ggcaagcaga ttgctgacca atatgggctc ccgaacggcg tcgaggtgac cgacgacgtg ttcgaatccg acgcttcgat cgtcttcgag caggcggaga accgccttca tacgatcaag gcgattctgg tcgctacgct caccggctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BuxeA.00088.a.A1.PS01326
| PURIFICATION DATe: | 1/4/2012 |
| CONCENTRATION: | 33.87mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMFNVHNR SYLTLIEYTP RQIRYLLDLS RDLKRAKYAG TETPRLNGKN IALIFEKTST RTRCAFEVAA HDQGAHVTYI DPNGSQIGHK ESMKDTARVL GRMYDAIEYR GFGQEIVEEL AKYAGVPVYN GLTDEFHPTQ MLADVLTMHE FSDRPIHDIA YCYIGDAHNN TGNSLMIVGA KLGMDVRLCA PRCLWPHDEL IEQCRAIAAT TGARLTLTEK PEEAVKGVDF IYTDVWVSMG EPFEKWGARI EELLPYRVNA ALLAASGNPR VKFMHCLPAF HDSNTHVGKQ IADQYGLPNG VEVTDDVFES DASIVFEQAE NRLHTIKAIL VATLTG
Validated NT Sequence
tattagccgg tgagcgtagc gaccagaatc gccttgatcg tatgaaggcg gttctccgcc tgctcgaaga cgatcgaagc gtcggattcg aacacgtcgt cggtcacctc gacgccgttc gggagcccat attggtcagc aatctgcttg ccgacgtggg tgttcgagtc atgaaacgcc ggcaggcaat gcatgaactt cacccgcgga ttaccacttg cagccagcag tgcggcattc acccggtacg gcaaaagctc ctcgatccgc gcgccccact tctcgaacgg ctcgcccatc gatacccata cgtcggtata gatgaagtcg acgcctttca cggcctcctc cggcttttcg gtcaacgtca ggcgcgcgcc cgtcgtggcc gcgattgccc ggcactgttc gatcagttca tcgtgcggcc acaggcagcg cggagcacac aggcggacat ccatgccgag cttggcgccc acaatcatca gcgaattgcc agtattgttg tgcgcgtcgc ctatgtagca ataggcgatg tcgtggatcg gcctgtcgct gaactcgtgc atcgttagca cgtcggccag catctgcgtc gggtggaact cgtcagtcaa gccgttgtag acaggcacgc cggcatactt cgcaagctct tcgacgatct cctgaccgaa tccacgatat tcgatcgcgt catacatgcg gcccagcacc cgggcggtgt ccttcatcga ttccttgtgg ccgatctgcg agccgttcgg gtcgatatag gtgacatggg cgccctggtc gtgcgccgcc acttcgaatg cacaacgcgt gcgcgtcgag gtcttctcaa agatcagcgc gatattcttg ccgttcaggc ggggcgtttc ggttccggca tacttggcgc gcttcagatc gcgggacagg tcgagcaggt agcgaatctg gcgaggcgta tattcgatca gcgtgaggta gctgcggttg tgtacgttga acatcgaacc aggaccctgg gtctgagctt ccagggtacc cata
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFNVHNRSY LTLIEYTPRQ IRYLLDLSRD LKRAKYAGTE TPRLNGKNIA LIFEKTSTRT RCAFEVAAHD QGAHVTYIDP NGSQIGHKES MKDTARVLGR MYDAIEYRGF GQEIVEELAK YAGVPVYNGL TDEFHPTQML ADVLTMHEFS DRPIHDIAYC YIGDAHNNTG NSLMIVGAKL GMDVRLCAPR CLWPHDELIE QCRAIAATTG ARLTLTEKPE EAVKGVDFIY TDVWVSMGEP FEKWGARIEE LLPYRVNAAL LAASGNPRVK FMHCLPAFHD SNTHVGKQIA DQYGLPNGVE VTDDVFESDA SIVFEQAENR LHTIKAILVA TLTG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTCAACGTA CACAACCGCA GCTACCTCAC GCTGATCGAA TATACGCCTC GCCAGATTCG CTACCTGCTC GACCTGTCCC GCGATCTGAA GCGCGCCAAG TATGCCGGAA CCGAAACGCC CCGCCTGAAC GGCAAGAATA TCGCGCTGAT CTTTGAGAAG ACCTCGACGC GCACGCGTTG TGCATTCGAA GTGGCGGCGC ACGACCAGGG CGCCCATGTC ACCTATATCG ACCCGAACGG CTCGCAGATC GGCCACAAGG AATCGATGAA GGACACCGCC CGGGTGCTGG GCCGCATGTA TGACGCGATC GAATATCGTG GATTCGGTCA GGAGATCGTC GAAGAGCTTG CGAAGTATGC CGGCGTGCCT GTCTACAACG GCTTGACTGA CGAGTTCCAC CCGACGCAGA TGCTGGCCGA CGTGCTAACG ATGCACGAGT TCAGCGACAG GCCGATCCAC GACATCGCCT ATTGCTACAT AGGCGACGCG CACAACAATA CTGGCAATTC GCTGATGATT GTGGGCGCCA AGCTCGGCAT GGATGTCCGC CTGTGTGCTC CGCGCTGCCT GTGGCCGCAC GATGAACTGA TCGAACAGTG CCGGGCAATC GCGGCCACGA CGGGCGCGCG CCTGACGTTG ACCGAAAAGC CGGAGGAGGC CGTGAAAGGC GTCGACTTCA TCTATACCGA CGTATGGGTA TCGATGGGCG AGCCGTTCGA GAAGTGGGGC GCGCGGATCG AGGAGCTTTT GCCGTACCGG GTGAATGCCG CACTGCTGGC TGCAAGTGGT AATCCGCGGG TGAAGTTCAT GCATTGCCTG CCGGCGTTTC ATGACTCGAA CACCCACGTC GGCAAGCAGA TTGCTGACCA ATATGGGCTC CCGAACGGCG TCGAGGTGAC CGACGACGTG TTCGAATCCG ACGCTTCGAT CGTCTTCGAG CAGGCGGAGA ACCGCCTTCA TACGATCAAG GCGATTCTGG TCGCTACGCT CACCGGCTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA
Details for BuxeA.00088.a.B2.PW39423
| PURIFICATION DATe: | 5/2/2025 |
| CONCENTRATION: | 24.5mg/ml |
| OBSERVED MW: | 34kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 23 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHFN VHNRSYLTLI EYTPRQIRYL LDLSRDLKRA KYAGTETPRL NGKNIALIFE KTSTRTRCAF EVAAHDQGAH VTYIDPNGSQ IGHKESMKDT ARVLGRMYDA IEYRGFGQEI VEELAKYAGV PVYNGLTDEF HPTQMLADVL TMHEFSDRPI HDIAYCYIGD AHNNTGNSLM IVGAKLGMDV RLCAPRCLWP HDELIEQCRA IAATTGARLT LTEKPEEAVK GVDFIYTDVW VSMGEPFEKW GARIEELLPY RVNAALLAAS GNPRVKFMHC LPAFHDSNTH VGKQIADQYG LPNGVEVTDD VFESDASIVF EQAENRLHTI KAILVATLT
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTCAACG TACACAACCG CAGCTACCTC ACGCTGATCG AATATACGCC TCGCCAGATT CGCTACCTGC TCGACCTGTC CCGCGATCTG AAGCGCGCCA AGTATGCCGG AACCGAAACG CCCCGCCTGA ACGGCAAGAA TATCGCGCTG ATCTTTGAGA AGACCTCGAC GCGCACGCGT TGTGCATTCG AAGTGGCGGC GCACGACCAG GGCGCCCATG TCACCTATAT CGACCCGAAC GGCTCGCAGA TCGGCCACAA GGAATCGATG AAGGACACCG CCCGGGTGCT GGGCCGCATG TATGACGCGA TCGAATATCG TGGATTCGGT CAGGAGATCG TCGAAGAGCT TGCGAAGTAT GCCGGCGTGC CTGTCTACAA CGGCTTGACT GACGAGTTCC ACCCGACGCA GATGCTGGCC GACGTGCTAA CGATGCACGA GTTCAGCGAC AGGCCGATCC ACGACATCGC CTATTGCTAC ATAGGCGACG CGCACAACAA TACTGGCAAT TCGCTGATGA TTGTGGGCGC CAAGCTCGGC ATGGATGTCC GCCTGTGTGC TCCGCGCTGC CTGTGGCCGC ACGATGAACT GATCGAACAG TGCCGGGCAA TCGCGGCCAC GACGGGCGCG CGCCTGACGT TGACCGAAAA GCCGGAGGAG GCCGTGAAAG GCGTCGACTT CATCTATACC GACGTATGGG TATCGATGGG CGAGCCGTTC GAGAAGTGGG GCGCGCGGAT CGAGGAGCTT TTGCCGTACC GGGTGAATGC CGCACTGCTG GCTGCAAGTG GTAATCCGCG GGTGAAGTTC ATGCATTGCC TGCCGGCGTT TCATGACTCG AACACCCACG TCGGCAAGCA GATTGCTGAC CAATATGGGC TCCCGAACGG CGTCGAGGTG ACCGACGACG TGTTCGAATC CGACGCTTCG ATCGTCTTCG AGCAGGCGGA GAACCGCCTT CATACGATCA AGGCGATTCT GGTCGCTACG CTCACCTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T