BuxeA.00085.c

Putative epimerase/dehydratase

CENTER ID: BuxeA.00085.c
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00085.c.B2.GE39439 Mature protein( BuxeA.00085.c ) 17 384
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.753
RefSeq: YP_557320.1
UniProt: Q144H1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKVLITGGAG FIGSSLARKL CASNAEVTVL DNLSPQIHGD NPDVTSAIFA SLPQSVHFVK GSVTDRAMLE RVMEGQDAIV HLAAETGTGQ SMYEVDRYVD VNVHGTALIL DILVKNSSNT VRNVVVASSR AIYGEGKYRS PAAGILYPGP RKEKDMLEGQ FEHLCPISGE PMECVPTDEE SKIHPTSLYG ITKQTQEQMV MTTCRSIGIA ASALRYQNVY GPGQSLSNPY TGILSIFSTR IKNGNPINIF EDGLESRDFV YIDDVVAATY SALTNPNAAD NVFGIGSGVR TSVIDVARSL CEAYGSNVDI SVTGAFRLGD VRHVYADLAR AREMLGFEPK VSFSEGIAKF AQWVDQQQVM ADTYEKSIAE MREKGLYKGC ERAA
NT Sequence
ATGAAAGTCC TCATTACGGG TGGCGCCGGC TTTATCGGCT CGAGTCTGGC GCGTAAGCTC TGCGCAAGCA ATGCGGAAGT GACGGTGCTC GATAATCTCT CGCCGCAGAT TCACGGAGAC AATCCGGATG TCACGTCCGC GATCTTTGCG TCGTTGCCGC AAAGCGTGCA CTTCGTGAAG GGATCGGTGA CGGATCGGGC CATGCTCGAA CGTGTGATGG AGGGGCAAGA CGCGATCGTC CATCTGGCGG CCGAGACAGG CACGGGACAA TCCATGTACG AGGTCGACCG TTACGTCGAT GTGAACGTGC ATGGCACGGC GCTGATTCTC GACATACTGG TCAAGAACAG CAGCAATACC GTGCGCAACG TGGTCGTCGC TTCGTCGCGA GCGATTTATG GCGAGGGCAA GTACCGTTCT CCGGCCGCCG GCATTCTGTA TCCCGGCCCG CGCAAGGAAA AAGACATGCT GGAGGGGCAA TTCGAGCACC TCTGCCCGAT TAGCGGCGAG CCGATGGAAT GCGTGCCGAC CGACGAGGAA TCGAAAATCC ATCCCACATC GCTCTACGGC ATTACCAAGC AGACTCAGGA ACAGATGGTG ATGACGACCT GCCGCTCCAT CGGCATCGCT GCGTCGGCCT TGCGTTATCA GAACGTCTAT GGGCCGGGGC AGTCGCTGTC CAATCCCTAC ACCGGTATTC TTTCGATCTT TTCGACGCGC ATCAAGAACG GCAATCCGAT CAATATCTTC GAGGACGGCC TGGAAAGCCG TGACTTCGTT TATATCGACG ACGTGGTCGC CGCAACGTAC TCCGCACTCA CCAATCCCAA TGCCGCGGAC AACGTATTCG GCATCGGCTC GGGCGTGCGC ACTTCGGTGA TCGACGTTGC GCGGTCGCTG TGCGAGGCGT ATGGCAGCAA CGTCGACATC AGCGTAACGG GCGCCTTCCG CCTTGGCGAT GTCCGCCATG TCTACGCTGA CCTGGCGAGA GCAAGGGAAA TGCTCGGCTT TGAGCCGAAA GTGTCGTTCA GCGAAGGCAT CGCGAAGTTT GCCCAATGGG TCGACCAGCA GCAGGTAATG GCCGACACTT ACGAGAAGAG TATTGCGGAG ATGCGGGAGA AAGGCCTGTA CAAGGGCTGT GAACGGGCGG CA
Details for BuxeA.00085.c.B2.GE39439
HARVESTED ON: 8/5/2015
SEQUENCED ON: 8/13/2015
EXPECTED MW: 41kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHAR KLCASNAEVT VLDNLSPQIH GDNPDVTSAI FASLPQSVHF VKGSVTDRAM LERVMEGQDA IVHLAAETGT GQSMYEVDRY VDVNVHGTAL ILDILVKNSS NTVRNVVVAS SRAIYGEGKY RSPAAGILYP GPRKEKDMLE GQFEHLCPIS GEPMECVPTD EESKIHPTSL YGITKQTQEQ MVMTTCRSIG IAASALRYQN VYGPGQSLSN PYTGILSIFS TRIKNGNPIN IFEDGLESRD FVYIDDVVAA TYSALTNPNA ADNVFGIGSG VRTSVIDVAR SLCEAYGSNV DISVTGAFRL GDVRHVYADL ARAREMLGFE PKVSFSEGIA KFAQWVDQQQ VMADTYEKSI AEMREKGLYK GCERAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgcgta agctctgcgc aagcaatgcg gaagtgacgg tgctcgataa tctctcgccg cagattcacg gagacaatcc ggatgtcacg tccgcgatct ttgcgtcgtt gccgcaaagc gtgcacttcg tgaagggatc ggtgacggat cgggccatgc tcgaacgtgt gatggagggg caagacgcga tcgtccatct ggcggccgag acaggcacgg gacaatccat gtacgaggtc gaccgttacg tcgatgtgaa cgtgcatggc acggcgctga ttctcgacat actggtcaag aacagcagca ataccgtgcg caacgtggtc gtcgcttcgt cgcgagcgat ttatggcgag ggcaagtacc gttctccggc cgccggcatt ctgtatcccg gcccgcgcaa ggaaaaagac atgctggagg ggcaattcga gcacctctgc ccgattagcg gcgagccgat ggaatgcgtg ccgaccgacg aggaatcgaa aatccatccc acatcgctct acggcattac caagcagact caggaacaga tggtgatgac gacctgccgc tccatcggca tcgctgcgtc ggccttgcgt tatcagaacg tctatgggcc ggggcagtcg ctgtccaatc cctacaccgg tattctttcg atcttttcga cgcgcatcaa gaacggcaat ccgatcaata tcttcgagga cggcctggaa agccgtgact tcgtttatat cgacgacgtg gtcgccgcaa cgtactccgc actcaccaat cccaatgccg cggacaacgt attcggcatc ggctcgggcg tgcgcacttc ggtgatcgac gttgcgcggt cgctgtgcga ggcgtatggc agcaacgtcg acatcagcgt aacgggcgcc ttccgccttg gcgatgtccg ccatgtctac gctgacctgg cgagagcaag ggaaatgctc ggctttgagc cgaaagtgtc gttcagcgaa ggcatcgcga agtttgccca atgggtcgac cagcagcagg taatggccga cacttacgag aagagtattg cggagatgcg ggagaaaggc ctgtacaagg gctgtgaacg ggcggcatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at