BuxeA.00077.d
Fumarylacetoacetate hydrolase
| CENTER ID: | BuxeA.00077.d |
| ORGANISM: | Burkholderia xenovorans LB400 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuxeA.00077.d.B1.GE39496 | Full length( BuxeA.00077.d ) | 1 | 436 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuxeA.00077.d.B1.PS37875 | Full length( BuxeA.00077.d ) | 1 | 436 |
Structures
5TI1
DEPOSITED: 9/30/2016
DETERMINATION: XRay
CLONE: BuxeA.00077.d.B1.GE39496
PROTEIN: BuxeA.00077.d.B1.PS37875
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|266265.5.peg.585 |
| RefSeq: | YP_557150.1 |
| UniProt: | Q144Z1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNASSDLQAT LDPSRKSWVE SANNPTGDFS IQNLPFGIFS DGLNATRRVG VAIGDSIVDL AALESAGLLS VPSTGAGDSV FVRDALNDFI ALGRDAWRSV RVQLSRLLSR DDATLRDDAE LRGRALIRQA DAQLHLPVQI PGYTDFYSSK EHATNVGSMF RDPKNALLPN WSEMPIGYNG RASSVVVSGT PVRRPNGQLK LPDQERPVFG ACRKLDIELE TGFVIGAGNA LGEPVTCADA EAHIFGMVLL NDWSARDIQQ WEYVPLGPFN AKTFATTISP WIVTLDALEP FRVAQPAQDP QPLAYLRHDG EHAFDITLEV TLRPQQAKEA STITRTNFKH MYWTMAQQLA HHTVSGCNTR VGDLMGSGTI SGPTEDSFGS LLELTWNGKK PLELREGGTR SFIEDGDELT LAGWCQGEGY RVGFGVCAGE ILPALK
NT Sequence
ATGAACGCAT CGAGCGATCT TCAGGCGACG CTCGATCCGT CGCGCAAAAG CTGGGTCGAG TCGGCCAACA ATCCGACCGG CGATTTTTCG ATTCAGAATC TGCCGTTCGG CATTTTCAGC GACGGGCTGA ATGCGACGCG CCGCGTGGGC GTGGCGATCG GCGACAGCAT CGTCGACCTG GCCGCGCTCG AAAGCGCGGG TCTGCTGAGC GTGCCGTCAA CGGGCGCGGG CGACAGCGTG TTCGTGCGCG ACGCGTTGAA CGATTTCATC GCCCTCGGCC GTGACGCATG GCGCAGTGTG CGTGTCCAGT TGAGCAGGCT GTTGTCGCGC GACGACGCAA CGCTGCGCGA CGACGCCGAG CTGCGCGGCC GCGCGCTGAT CCGTCAGGCC GACGCGCAAT TGCATCTGCC GGTGCAGATT CCGGGCTACA CCGATTTCTA TTCGTCGAAG GAGCACGCAA CGAATGTCGG CTCCATGTTC CGCGATCCGA AGAATGCGCT GCTGCCGAAC TGGTCGGAGA TGCCGATTGG TTACAACGGA CGCGCGTCGT CGGTGGTGGT GAGCGGCACG CCCGTGCGTC GTCCTAACGG ACAGCTGAAG CTGCCGGATC AGGAGCGTCC GGTGTTCGGC GCGTGCCGCA AGCTGGATAT CGAACTGGAA ACGGGCTTCG TGATCGGTGC GGGCAATGCC CTGGGCGAGC CGGTTACATG CGCGGATGCC GAAGCGCATA TCTTCGGCAT GGTGCTGCTG AACGACTGGA GCGCGCGCGA TATCCAGCAG TGGGAATACG TGCCGCTCGG GCCGTTCAAC GCGAAGACCT TCGCGACCAC GATCTCGCCC TGGATCGTGA CGCTCGACGC GCTCGAACCG TTCCGCGTCG CGCAGCCCGC GCAAGATCCG CAGCCGCTCG CTTATCTGCG GCACGACGGC GAACATGCTT TCGACATCAC GCTGGAAGTG ACGCTACGCC CGCAGCAGGC GAAAGAGGCG AGCACGATCA CGCGGACCAA CTTCAAACAC ATGTACTGGA CGATGGCGCA GCAACTCGCG CATCACACGG TATCGGGCTG CAACACGCGC GTGGGCGACC TGATGGGCTC CGGCACGATC AGCGGGCCGA CGGAGGATTC CTTCGGCAGC CTGCTCGAAT TGACGTGGAA CGGCAAGAAG CCGCTGGAGT TGCGCGAAGG CGGCACGCGC AGTTTCATCG AAGACGGCGA CGAGTTGACG CTTGCCGGCT GGTGCCAGGG CGAGGGTTAT CGGGTGGGCT TCGGCGTGTG CGCCGGGGAG ATTTTGCCGG CGTTGAAG
Details for BuxeA.00077.d.B1.GE39496
| HARVESTED ON: | 9/10/2015 |
| SEQUENCED ON: | 9/16/2015 |
| EXPECTED MW: | 48kDa |
| OBSERVED MW: | 48kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMN AXSDLQATLD PSRKSWVESA NNPTGDFSIQ NLPFGIFSDG LNATRRVGVA IGDSIVDLAA LESAGLLSVP STGAGDSVFV RDALNDFIAL GRDAWRSVRV QLSRLLSRDD ATLRDDAELR GRALIRQADA QLHLPVQIPG YTDFYSSKEH ATNVGSMFRD PKNALLPNWS EMPIGYNGRA SSVVVSGTPV RRPNGQLKLP DQERPVFGAC RKLDIELETG FVIGAGNALG EPVTCADAEA HIFGMVLLND WSARDIQQWE YVPLGPFNAK TFATTISPWI VTLDALEPFR VAQPAQDPQP LAYLRHDGEH AFDITLEVTL RPQQAKEAST ITRTNFKHMY WTMAQQLAHH TVSGCNTRVG DLMGSGTISG PTEDSFGSLL ELTWNGKKPL ELREGGTRSF IEDGDELTLA GWCQGEGYRV GFGVC
Validated NT Sequence
ggcgcacacg ccgaagccca cccgataacc ctcgccctgg caccagccgg caagcgtcaa ctcgtcgccg tcttcgatga aactgcgcgt gccgccttcg cgcaactcca gcggcttctt gccgttccac gtcaattcga gcaggctgcc gaaggaatcc tccgtcggcc cgctgatcgt gccggagccc atcaggtcgc ccacgcgcgt gttgcagccc gataccgtgt gatgcgcgag ttgctgcgcc atcgtccagt acatgtgttt gaagttggtc cgcgtgatcg tgctcgcctc tttcgcctgc tgcgggcgta gcgtcacttc cagcgtgatg tcgaaagcat gttcgccgtc gtgccgcaga taagcgagcg gctgcggatc ttgcgcgggc tgcgcgacgc ggaacggttc gagcgcgtcg agcgtcacga tccagggcga gatcgtggtc gcgaaggtct tcgcgttgaa cggcccgagc ggcacgtatt cccactgctg gatatcgcgc gcgctccagt cgttcagcag caccatgccg aagatatgcg cttcggcatc cgcgcatgta accggctcgc ccagggcatt gcccgcaccg atcacgaagc ccgtttccag ttcgatatcc agcttgcggc acgcgccgaa caccggacgc tcctgatccg gcagcttcag ctgtccgtta ggacgacgca cgggcgtgcc gctcaccacc accgacgacg cgcgtccgtt gtaaccaatc ggcatctccg accagttcgg cagcagcgca ttcttcggat cgcggaacat ggagccgaca ttcgttgcgt gctccttcga cgaatagaaa tcggtgtagc ccggaatctg caccggcaga tgcaattgcg cgtcggcctg acggatcagc gcgcggccgc gcagctcggc gtcgtcgcgc agcgttgcgt cgtcgcgcga caacagcctg ctcaactgga cacgcacact gcgccatgcg tcacggccga gggcgatgaa atcgttcaac gcgtcgcgca cgaacacgct gtcgcccgcg cccgttgacg gcacgctcag cagacccgcg ctttcgagcg cggccaggtc gacgatgctg tcgccgatcg ccacgcccac gcggcgcgtc gcattcagcc cgtcgctgaa aatgccgaac ggcagattct gaatcgaaaa atcgccggtc ggattgttgg ccgactcgac ccagcttttg cgcgacggat cgagcgtcgc ctgaagatcg ctnnntgcgt tcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMN ASSDLQATLD PSRKSWVESA NNPTGDFSIQ NLPFGIFSDG LNATRRVGVA IGDSIVDLAA LESAGLLSVP STGAGDSVFV RDALNDFIAL GRDAWRSVRV QLSRLLSRDD ATLRDDAELR GRALIRQADA QLHLPVQIPG YTDFYSSKEH ATNVGSMFRD PKNALLPNWS EMPIGYNGRA SSVVVSGTPV RRPNGQLKLP DQERPVFGAC RKLDIELETG FVIGAGNALG EPVTCADAEA HIFGMVLLND WSARDIQQWE YVPLGPFNAK TFATTISPWI VTLDALEPFR VAQPAQDPQP LAYLRHDGEH AFDITLEVTL RPQQAKEAST ITRTNFKHMY WTMAQQLAHH TVSGCNTRVG DLMGSGTISG PTEDSFGSLL ELTWNGKKPL ELREGGTRSF IEDGDELTLA GWCQGEGYRV GFGVCAGEIL PALK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg catcgagcga tcttcaggcg acgctcgatc cgtcgcgcaa aagctgggtc gagtcggcca acaatccgac cggcgatttt tcgattcaga atctgccgtt cggcattttc agcgacgggc tgaatgcgac gcgccgcgtg ggcgtggcga tcggcgacag catcgtcgac ctggccgcgc tcgaaagcgc gggtctgctg agcgtgccgt caacgggcgc gggcgacagc gtgttcgtgc gcgacgcgtt gaacgatttc atcgccctcg gccgtgacgc atggcgcagt gtgcgtgtcc agttgagcag gctgttgtcg cgcgacgacg caacgctgcg cgacgacgcc gagctgcgcg gccgcgcgct gatccgtcag gccgacgcgc aattgcatct gccggtgcag attccgggct acaccgattt ctattcgtcg aaggagcacg caacgaatgt cggctccatg ttccgcgatc cgaagaatgc gctgctgccg aactggtcgg agatgccgat tggttacaac ggacgcgcgt cgtcggtggt ggtgagcggc acgcccgtgc gtcgtcctaa cggacagctg aagctgccgg atcaggagcg tccggtgttc ggcgcgtgcc gcaagctgga tatcgaactg gaaacgggct tcgtgatcgg tgcgggcaat gccctgggcg agccggttac atgcgcggat gccgaagcgc atatcttcgg catggtgctg ctgaacgact ggagcgcgcg cgatatccag cagtgggaat acgtgccgct cgggccgttc aacgcgaaga ccttcgcgac cacgatctcg ccctggatcg tgacgctcga cgcgctcgaa ccgttccgcg tcgcgcagcc cgcgcaagat ccgcagccgc tcgcttatct gcggcacgac ggcgaacatg ctttcgacat cacgctggaa gtgacgctac gcccgcagca ggcgaaagag gcgagcacga tcacgcggac caacttcaaa cacatgtact ggacgatggc gcagcaactc gcgcatcaca cggtatcggg ctgcaacacg cgcgtgggcg acctgatggg ctccggcacg atcagcgggc cgacggagga ttccttcggc agcctgctcg aattgacgtg gaacggcaag aagccgctgg agttgcgcga aggcggcacg cgcagtttca tcgaagacgg cgacgagttg acgcttgccg gctggtgcca gggcgagggt tatcgggtgg gcttcggcgt gtgcgccggg gagattttgc cggcgttgaa gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for BuxeA.00077.d.B1.PS37875
| PURIFICATION DATe: | 3/8/2016 |
| CONCENTRATION: | 67mg/ml |
| OBSERVED MW: | 47kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | SEC buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN AXSDLQATLD PSRKSWVESA NNPTGDFSIQ NLPFGIFSDG LNATRRVGVA IGDSIVDLAA LESAGLLSVP STGAGDSVFV RDALNDFIAL GRDAWRSVRV QLSRLLSRDD ATLRDDAELR GRALIRQADA QLHLPVQIPG YTDFYSSKEH ATNVGSMFRD PKNALLPNWS EMPIGYNGRA SSVVVSGTPV RRPNGQLKLP DQERPVFGAC RKLDIELETG FVIGAGNALG EPVTCADAEA HIFGMVLLND WSARDIQQWE YVPLGPFNAK TFATTISPWI VTLDALEPFR VAQPAQDPQP LAYLRHDGEH AFDITLEVTL RPQQAKEAST ITRTNFKHMY WTMAQQLAHH TVSGCNTRVG DLMGSGTISG PTEDSFGSLL ELTWNGKKPL ELREGGTRSF IEDGDELTLA GWCQGEGYRV GFGVC
Validated NT Sequence
ggcgcacacg ccgaagccca cccgataacc ctcgccctgg caccagccgg caagcgtcaa ctcgtcgccg tcttcgatga aactgcgcgt gccgccttcg cgcaactcca gcggcttctt gccgttccac gtcaattcga gcaggctgcc gaaggaatcc tccgtcggcc cgctgatcgt gccggagccc atcaggtcgc ccacgcgcgt gttgcagccc gataccgtgt gatgcgcgag ttgctgcgcc atcgtccagt acatgtgttt gaagttggtc cgcgtgatcg tgctcgcctc tttcgcctgc tgcgggcgta gcgtcacttc cagcgtgatg tcgaaagcat gttcgccgtc gtgccgcaga taagcgagcg gctgcggatc ttgcgcgggc tgcgcgacgc ggaacggttc gagcgcgtcg agcgtcacga tccagggcga gatcgtggtc gcgaaggtct tcgcgttgaa cggcccgagc ggcacgtatt cccactgctg gatatcgcgc gcgctccagt cgttcagcag caccatgccg aagatatgcg cttcggcatc cgcgcatgta accggctcgc ccagggcatt gcccgcaccg atcacgaagc ccgtttccag ttcgatatcc agcttgcggc acgcgccgaa caccggacgc tcctgatccg gcagcttcag ctgtccgtta ggacgacgca cgggcgtgcc gctcaccacc accgacgacg cgcgtccgtt gtaaccaatc ggcatctccg accagttcgg cagcagcgca ttcttcggat cgcggaacat ggagccgaca ttcgttgcgt gctccttcga cgaatagaaa tcggtgtagc ccggaatctg caccggcaga tgcaattgcg cgtcggcctg acggatcagc gcgcggccgc gcagctcggc gtcgtcgcgc agcgttgcgt cgtcgcgcga caacagcctg ctcaactgga cacgcacact gcgccatgcg tcacggccga gggcgatgaa atcgttcaac gcgtcgcgca cgaacacgct gtcgcccgcg cccgttgacg gcacgctcag cagacccgcg ctttcgagcg cggccaggtc gacgatgctg tcgccgatcg ccacgcccac gcggcgcgtc gcattcagcc cgtcgctgaa aatgccgaac ggcagattct gaatcgaaaa atcgccggtc ggattgttgg ccgactcgac ccagcttttg cgcgacggat cgagcgtcgc ctgaagatcg ctnnntgcgt tcatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHMN ASSDLQATLD PSRKSWVESA NNPTGDFSIQ NLPFGIFSDG LNATRRVGVA IGDSIVDLAA LESAGLLSVP STGAGDSVFV RDALNDFIAL GRDAWRSVRV QLSRLLSRDD ATLRDDAELR GRALIRQADA QLHLPVQIPG YTDFYSSKEH ATNVGSMFRD PKNALLPNWS EMPIGYNGRA SSVVVSGTPV RRPNGQLKLP DQERPVFGAC RKLDIELETG FVIGAGNALG EPVTCADAEA HIFGMVLLND WSARDIQQWE YVPLGPFNAK TFATTISPWI VTLDALEPFR VAQPAQDPQP LAYLRHDGEH AFDITLEVTL RPQQAKEAST ITRTNFKHMY WTMAQQLAHH TVSGCNTRVG DLMGSGTISG PTEDSFGSLL ELTWNGKKPL ELREGGTRSF IEDGDELTLA GWCQGEGYRV GFGVCAGEIL PALK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACG CATCGAGCGA TCTTCAGGCG ACGCTCGATC CGTCGCGCAA AAGCTGGGTC GAGTCGGCCA ACAATCCGAC CGGCGATTTT TCGATTCAGA ATCTGCCGTT CGGCATTTTC AGCGACGGGC TGAATGCGAC GCGCCGCGTG GGCGTGGCGA TCGGCGACAG CATCGTCGAC CTGGCCGCGC TCGAAAGCGC GGGTCTGCTG AGCGTGCCGT CAACGGGCGC GGGCGACAGC GTGTTCGTGC GCGACGCGTT GAACGATTTC ATCGCCCTCG GCCGTGACGC ATGGCGCAGT GTGCGTGTCC AGTTGAGCAG GCTGTTGTCG CGCGACGACG CAACGCTGCG CGACGACGCC GAGCTGCGCG GCCGCGCGCT GATCCGTCAG GCCGACGCGC AATTGCATCT GCCGGTGCAG ATTCCGGGCT ACACCGATTT CTATTCGTCG AAGGAGCACG CAACGAATGT CGGCTCCATG TTCCGCGATC CGAAGAATGC GCTGCTGCCG AACTGGTCGG AGATGCCGAT TGGTTACAAC GGACGCGCGT CGTCGGTGGT GGTGAGCGGC ACGCCCGTGC GTCGTCCTAA CGGACAGCTG AAGCTGCCGG ATCAGGAGCG TCCGGTGTTC GGCGCGTGCC GCAAGCTGGA TATCGAACTG GAAACGGGCT TCGTGATCGG TGCGGGCAAT GCCCTGGGCG AGCCGGTTAC ATGCGCGGAT GCCGAAGCGC ATATCTTCGG CATGGTGCTG CTGAACGACT GGAGCGCGCG CGATATCCAG CAGTGGGAAT ACGTGCCGCT CGGGCCGTTC AACGCGAAGA CCTTCGCGAC CACGATCTCG CCCTGGATCG TGACGCTCGA CGCGCTCGAA CCGTTCCGCG TCGCGCAGCC CGCGCAAGAT CCGCAGCCGC TCGCTTATCT GCGGCACGAC GGCGAACATG CTTTCGACAT CACGCTGGAA GTGACGCTAC GCCCGCAGCA GGCGAAAGAG GCGAGCACGA TCACGCGGAC CAACTTCAAA CACATGTACT GGACGATGGC GCAGCAACTC GCGCATCACA CGGTATCGGG CTGCAACACG CGCGTGGGCG ACCTGATGGG CTCCGGCACG ATCAGCGGGC CGACGGAGGA TTCCTTCGGC AGCCTGCTCG AATTGACGTG GAACGGCAAG AAGCCGCTGG AGTTGCGCGA AGGCGGCACG CGCAGTTTCA TCGAAGACGG CGACGAGTTG ACGCTTGCCG GCTGGTGCCA GGGCGAGGGT TATCGGGTGG GCTTCGGCGT GTGCGCCGGG GAGATTTTGC CGGCGTTGAA GTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT