BuxeA.00077.c

Fumarylacetoacetate hydrolase

CENTER ID: BuxeA.00077.c
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00077.c.B1.GE39495 Full length( BuxeA.00077.c ) 1 437
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00077.c.B1.PS37867 Full length( BuxeA.00077.c ) 1 437
BuxeA.00077.c.B1.PS37868 Full length( BuxeA.00077.c ) 1 437
BuxeA.00077.c.B1.PS38171 Full length( BuxeA.00077.c ) 1 437

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.1770
RefSeq: YP_558292.1
UniProt: Q140J9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVLNETHDP SLKSWVASAN AAGSDFPIQN LPFAVFRRNG SPEAFRGGVA IGEMIVDLGA VARLLDGVVA DAARAGSESS LNALMAQGSA AWSALRLALS RALREGALQQ AAFANALVAQ ADAEYTVPAR IGDYTDFYTS IYHATNIGRL FRPDNPLMPN YKWVPIGYHG RASSIAVSGQ QFPRPVGQTF PPGSEQPSFG PSKRLDYELE LAVYIGTGNA LGSPIPIDDA EAHVFGIGLL NDWSARDVQA WEYQPLGPFL AKSFASTVSP WIVTLEALAP YRVAFERNAG DPQPLPYLDS VRQRERGAFD ITLELGIESA RLRSQGRPAA RVSLTNYRHA YWTIAQMVAH HTVNGCNLQP GDLLGTGTLS GPTLDQAGAL IETTSGGKNP FELGNGEART FLEDGDAVII RGWCEKAGAA RIGFGECRGA ILPARAV
NT Sequence
ATGTCCGTTC TCAACGAAAC CCACGATCCG AGTCTCAAGA GCTGGGTCGC CTCGGCCAAC GCGGCTGGTA GCGATTTTCC GATCCAGAAC TTGCCGTTCG CGGTGTTTCG CCGTAACGGC AGCCCGGAGG CGTTCCGCGG TGGCGTCGCG ATCGGCGAGA TGATCGTCGA TCTCGGCGCG GTCGCGCGCC TGCTAGATGG TGTGGTCGCC GACGCGGCCC GGGCCGGCAG CGAGAGCAGC CTCAATGCGC TGATGGCGCA GGGCTCGGCC GCGTGGTCGG CGCTACGGCT CGCGCTATCG CGCGCGCTGC GCGAGGGCGC GCTGCAGCAG GCCGCGTTCG CGAATGCGCT GGTCGCGCAG GCCGACGCCG AATACACGGT GCCCGCGCGG ATCGGCGACT ACACCGACTT CTATACCTCG ATCTACCATG CGACCAATAT CGGCAGGCTG TTCCGGCCGG ACAATCCGCT GATGCCGAAC TACAAGTGGG TACCGATCGG CTATCACGGC CGCGCCTCGT CGATCGCGGT GTCGGGCCAG CAGTTCCCGC GGCCGGTCGG GCAGACGTTT CCGCCGGGCT CGGAGCAGCC GAGCTTCGGG CCGAGCAAGC GGCTCGACTA CGAGCTCGAG CTCGCCGTCT ACATTGGCAC TGGCAACGCG CTCGGCAGTC CGATCCCGAT CGACGATGCG GAGGCGCATG TGTTCGGCAT CGGCCTCCTG AACGACTGGT CCGCCCGCGA CGTCCAGGCT TGGGAATATC AGCCGCTCGG GCCGTTCCTC GCGAAGAGCT TCGCGAGCAC GGTGTCTCCG TGGATCGTCA CGCTCGAGGC GCTCGCTCCG TACCGCGTGG CGTTCGAGCG CAACGCGGGC GATCCGCAGC CGCTGCCTTA CCTCGATTCG GTGCGGCAGC GCGAGCGGGG CGCATTCGAC ATCACGCTCG AGCTCGGGAT CGAATCGGCG CGGCTGCGCT CGCAGGGCCG GCCGGCCGCG CGCGTAAGCC TCACGAACTA CCGCCACGCG TACTGGACGA TCGCGCAGAT GGTCGCGCAT CACACGGTCA ACGGTTGCAA CCTGCAGCCG GGCGACCTGC TTGGCACGGG CACGCTGTCG GGTCCGACGC TGGACCAGGC GGGCGCGCTG ATCGAGACCA CGAGCGGAGG CAAGAACCCG TTCGAGCTCG GCAATGGCGA GGCGCGGACT TTCCTCGAGG ACGGCGATGC GGTGATCATC CGTGGATGGT GCGAGAAGGC GGGTGCGGCA CGCATCGGCT TCGGCGAGTG TCGTGGCGCG ATTTTGCCCG CGCGTGCGGT G
Details for BuxeA.00077.c.B1.GE39495
HARVESTED ON: 9/10/2015
SEQUENCED ON: 9/16/2015
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS VLNETHDPSL KSWVASANAA GSDFPIQNLP FAVFRRNGSP EAFRGGVAIG EMIVDLGAVA RLLDGVVADA ARAGSESSLN ALMAQGSAAW SALRLALSRA LREGALQQAA FANALVAQAD AEYTVPARIG DYTDFYTSIY HATNIGRLFR PDNPLMPNYK WVPIGYHGRA SSIAVSGQQF PRPVGQTFPP GSEQPSFGPS KRLDYELELA VYIGTGNALG SPIPIDDAEA HVFGIGLLND WSARDVQAWE YQPLGPFLAK SFASTVSPWI VTLEALAPYR VAFERNAGDP QPLPYLDSVR QRERGAFDIT LELGIESARL RSQGRPAARV SLTNYRHAYW TIAQMVAHHT VNGCNLQPGD LLGTGTLSGP TLDQAGALIE TTSGGKNPFE LGNGEARTFL EDGDAVIIRG WCEKAGAARI GFGECRGX
Validated NT Sequence
tnnngccacg acactcgccg aagccgatgc gtgccgcacc cgccttctcg caccatccac ggatgatcac cgcatcgccg tcctcgagga aagtccgcgc ctcgccattg ccgagctcga acgggttctt gcctccgctc gtggtctcga tcagcgcgcc cgcctggtcc agcgtcggac ccgacagcgt gcccgtgcca agcaggtcgc ccggctgcag gttgcaaccg ttgaccgtgt gatgcgcgac catctgcgcg atcgtccagt acgcgtggcg gtagttcgtg aggcttacgc gcgcggccgg ccggccctgc gagcgcagcc gcgccgattc gatcccgagc tcgagcgtga tgtcgaatgc gccccgctcg cgctgccgca ccgaatcgag gtaaggcagc ggctgcggat cgcccgcgtt gcgctcgaac gccacgcggt acggagcgag cgcctcgagc gtgacgatcc acggagacac cgtgctcgcg aagctcttcg cgaggaacgg cccgagcggc tgatattccc aagcctggac gtcgcgggcg gaccagtcgt tcaggaggcc gatgccgaac acatgcgcct ccgcatcgtc gatcgggatc ggactgccga gcgcgttgcc agtgccaatg tagacggcga gctcgagctc gtagtcgagc cgcttgctcg gcccgaagct cggctgctcc gagcccggcg gaaacgtctg cccgaccggc cgcgggaact gctggcccga caccgcgatc gacgaggcgc ggccgtgata gccgatcggt acccacttgt agttcggcat cagcggattg tccggccgga acagcctgcc gatattggtc gcatggtaga tcgaggtata gaagtcggtg tagtcgccga tccgcgcggg caccgtgtat tcggcgtcgg cctgcgcgac cagcgcattc gcgaacgcgg cctgctgcag cgcgccctcg cgcagcgcgc gcgatagcgc gagccgtagc gccgaccacg cggccgagcc ctgcgccatc agcgcattga ggctgctctc gctgccggcc cgggccgcgt cggcgaccac accatctagc aggcgcgcga ccgcgccgag atcgacgatc atctcgccga tcgcgacgcc accgcggaac gcctccgggc tgccgttacg gcgaaacacc gcgaacggca agttctggat cggaaaatcg ctaccagccg cgttggccga ggcgacccag ctcttgagac tcggatcgtg ggtttcgttg agaacggaca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMS VLNETHDPSL KSWVASANAA GSDFPIQNLP FAVFRRNGSP EAFRGGVAIG EMIVDLGAVA RLLDGVVADA ARAGSESSLN ALMAQGSAAW SALRLALSRA LREGALQQAA FANALVAQAD AEYTVPARIG DYTDFYTSIY HATNIGRLFR PDNPLMPNYK WVPIGYHGRA SSIAVSGQQF PRPVGQTFPP GSEQPSFGPS KRLDYELELA VYIGTGNALG SPIPIDDAEA HVFGIGLLND WSARDVQAWE YQPLGPFLAK SFASTVSPWI VTLEALAPYR VAFERNAGDP QPLPYLDSVR QRERGAFDIT LELGIESARL RSQGRPAARV SLTNYRHAYW TIAQMVAHHT VNGCNLQPGD LLGTGTLSGP TLDQAGALIE TTSGGKNPFE LGNGEARTFL EDGDAVIIRG WCEKAGAARI GFGECRGAIL PARAV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg ttctcaacga aacccacgat ccgagtctca agagctgggt cgcctcggcc aacgcggctg gtagcgattt tccgatccag aacttgccgt tcgcggtgtt tcgccgtaac ggcagcccgg aggcgttccg cggtggcgtc gcgatcggcg agatgatcgt cgatctcggc gcggtcgcgc gcctgctaga tggtgtggtc gccgacgcgg cccgggccgg cagcgagagc agcctcaatg cgctgatggc gcagggctcg gccgcgtggt cggcgctacg gctcgcgcta tcgcgcgcgc tgcgcgaggg cgcgctgcag caggccgcgt tcgcgaatgc gctggtcgcg caggccgacg ccgaatacac ggtgcccgcg cggatcggcg actacaccga cttctatacc tcgatctacc atgcgaccaa tatcggcagg ctgttccggc cggacaatcc gctgatgccg aactacaagt gggtaccgat cggctatcac ggccgcgcct cgtcgatcgc ggtgtcgggc cagcagttcc cgcggccggt cgggcagacg tttccgccgg gctcggagca gccgagcttc gggccgagca agcggctcga ctacgagctc gagctcgccg tctacattgg cactggcaac gcgctcggca gtccgatccc gatcgacgat gcggaggcgc atgtgttcgg catcggcctc ctgaacgact ggtccgcccg cgacgtccag gcttgggaat atcagccgct cgggccgttc ctcgcgaaga gcttcgcgag cacggtgtct ccgtggatcg tcacgctcga ggcgctcgct ccgtaccgcg tggcgttcga gcgcaacgcg ggcgatccgc agccgctgcc ttacctcgat tcggtgcggc agcgcgagcg gggcgcattc gacatcacgc tcgagctcgg gatcgaatcg gcgcggctgc gctcgcaggg ccggccggcc gcgcgcgtaa gcctcacgaa ctaccgccac gcgtactgga cgatcgcgca gatggtcgcg catcacacgg tcaacggttg caacctgcag ccgggcgacc tgcttggcac gggcacgctg tcgggtccga cgctggacca ggcgggcgcg ctgatcgaga ccacgagcgg aggcaagaac ccgttcgagc tcggcaatgg cgaggcgcgg actttcctcg aggacggcga tgcggtgatc atccgtggat ggtgcgagaa ggcgggtgcg gcacgcatcg gcttcggcga gtgtcgtggc gcgattttgc ccgcgcgtgc ggtgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BuxeA.00077.c.B1.PS37867
PURIFICATION DATe: 3/8/2016
CONCENTRATION: 58mg/ml
OBSERVED MW: 47kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS VLNETHDPSL KSWVASANAA GSDFPIQNLP FAVFRRNGSP EAFRGGVAIG EMIVDLGAVA RLLDGVVADA ARAGSESSLN ALMAQGSAAW SALRLALSRA LREGALQQAA FANALVAQAD AEYTVPARIG DYTDFYTSIY HATNIGRLFR PDNPLMPNYK WVPIGYHGRA SSIAVSGQQF PRPVGQTFPP GSEQPSFGPS KRLDYELELA VYIGTGNALG SPIPIDDAEA HVFGIGLLND WSARDVQAWE YQPLGPFLAK SFASTVSPWI VTLEALAPYR VAFERNAGDP QPLPYLDSVR QRERGAFDIT LELGIESARL RSQGRPAARV SLTNYRHAYW TIAQMVAHHT VNGCNLQPGD LLGTGTLSGP TLDQAGALIE TTSGGKNPFE LGNGEARTFL EDGDAVIIRG WCEKAGAARI GFGECRGX
Validated NT Sequence
tnnngccacg acactcgccg aagccgatgc gtgccgcacc cgccttctcg caccatccac ggatgatcac cgcatcgccg tcctcgagga aagtccgcgc ctcgccattg ccgagctcga acgggttctt gcctccgctc gtggtctcga tcagcgcgcc cgcctggtcc agcgtcggac ccgacagcgt gcccgtgcca agcaggtcgc ccggctgcag gttgcaaccg ttgaccgtgt gatgcgcgac catctgcgcg atcgtccagt acgcgtggcg gtagttcgtg aggcttacgc gcgcggccgg ccggccctgc gagcgcagcc gcgccgattc gatcccgagc tcgagcgtga tgtcgaatgc gccccgctcg cgctgccgca ccgaatcgag gtaaggcagc ggctgcggat cgcccgcgtt gcgctcgaac gccacgcggt acggagcgag cgcctcgagc gtgacgatcc acggagacac cgtgctcgcg aagctcttcg cgaggaacgg cccgagcggc tgatattccc aagcctggac gtcgcgggcg gaccagtcgt tcaggaggcc gatgccgaac acatgcgcct ccgcatcgtc gatcgggatc ggactgccga gcgcgttgcc agtgccaatg tagacggcga gctcgagctc gtagtcgagc cgcttgctcg gcccgaagct cggctgctcc gagcccggcg gaaacgtctg cccgaccggc cgcgggaact gctggcccga caccgcgatc gacgaggcgc ggccgtgata gccgatcggt acccacttgt agttcggcat cagcggattg tccggccgga acagcctgcc gatattggtc gcatggtaga tcgaggtata gaagtcggtg tagtcgccga tccgcgcggg caccgtgtat tcggcgtcgg cctgcgcgac cagcgcattc gcgaacgcgg cctgctgcag cgcgccctcg cgcagcgcgc gcgatagcgc gagccgtagc gccgaccacg cggccgagcc ctgcgccatc agcgcattga ggctgctctc gctgccggcc cgggccgcgt cggcgaccac accatctagc aggcgcgcga ccgcgccgag atcgacgatc atctcgccga tcgcgacgcc accgcggaac gcctccgggc tgccgttacg gcgaaacacc gcgaacggca agttctggat cggaaaatcg ctaccagccg cgttggccga ggcgacccag ctcttgagac tcggatcgtg ggtttcgttg agaacggaca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMS VLNETHDPSL KSWVASANAA GSDFPIQNLP FAVFRRNGSP EAFRGGVAIG EMIVDLGAVA RLLDGVVADA ARAGSESSLN ALMAQGSAAW SALRLALSRA LREGALQQAA FANALVAQAD AEYTVPARIG DYTDFYTSIY HATNIGRLFR PDNPLMPNYK WVPIGYHGRA SSIAVSGQQF PRPVGQTFPP GSEQPSFGPS KRLDYELELA VYIGTGNALG SPIPIDDAEA HVFGIGLLND WSARDVQAWE YQPLGPFLAK SFASTVSPWI VTLEALAPYR VAFERNAGDP QPLPYLDSVR QRERGAFDIT LELGIESARL RSQGRPAARV SLTNYRHAYW TIAQMVAHHT VNGCNLQPGD LLGTGTLSGP TLDQAGALIE TTSGGKNPFE LGNGEARTFL EDGDAVIIRG WCEKAGAARI GFGECRGAIL PARAV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCG TTCTCAACGA AACCCACGAT CCGAGTCTCA AGAGCTGGGT CGCCTCGGCC AACGCGGCTG GTAGCGATTT TCCGATCCAG AACTTGCCGT TCGCGGTGTT TCGCCGTAAC GGCAGCCCGG AGGCGTTCCG CGGTGGCGTC GCGATCGGCG AGATGATCGT CGATCTCGGC GCGGTCGCGC GCCTGCTAGA TGGTGTGGTC GCCGACGCGG CCCGGGCCGG CAGCGAGAGC AGCCTCAATG CGCTGATGGC GCAGGGCTCG GCCGCGTGGT CGGCGCTACG GCTCGCGCTA TCGCGCGCGC TGCGCGAGGG CGCGCTGCAG CAGGCCGCGT TCGCGAATGC GCTGGTCGCG CAGGCCGACG CCGAATACAC GGTGCCCGCG CGGATCGGCG ACTACACCGA CTTCTATACC TCGATCTACC ATGCGACCAA TATCGGCAGG CTGTTCCGGC CGGACAATCC GCTGATGCCG AACTACAAGT GGGTACCGAT CGGCTATCAC GGCCGCGCCT CGTCGATCGC GGTGTCGGGC CAGCAGTTCC CGCGGCCGGT CGGGCAGACG TTTCCGCCGG GCTCGGAGCA GCCGAGCTTC GGGCCGAGCA AGCGGCTCGA CTACGAGCTC GAGCTCGCCG TCTACATTGG CACTGGCAAC GCGCTCGGCA GTCCGATCCC GATCGACGAT GCGGAGGCGC ATGTGTTCGG CATCGGCCTC CTGAACGACT GGTCCGCCCG CGACGTCCAG GCTTGGGAAT ATCAGCCGCT CGGGCCGTTC CTCGCGAAGA GCTTCGCGAG CACGGTGTCT CCGTGGATCG TCACGCTCGA GGCGCTCGCT CCGTACCGCG TGGCGTTCGA GCGCAACGCG GGCGATCCGC AGCCGCTGCC TTACCTCGAT TCGGTGCGGC AGCGCGAGCG GGGCGCATTC GACATCACGC TCGAGCTCGG GATCGAATCG GCGCGGCTGC GCTCGCAGGG CCGGCCGGCC GCGCGCGTAA GCCTCACGAA CTACCGCCAC GCGTACTGGA CGATCGCGCA GATGGTCGCG CATCACACGG TCAACGGTTG CAACCTGCAG CCGGGCGACC TGCTTGGCAC GGGCACGCTG TCGGGTCCGA CGCTGGACCA GGCGGGCGCG CTGATCGAGA CCACGAGCGG AGGCAAGAAC CCGTTCGAGC TCGGCAATGG CGAGGCGCGG ACTTTCCTCG AGGACGGCGA TGCGGTGATC ATCCGTGGAT GGTGCGAGAA GGCGGGTGCG GCACGCATCG GCTTCGGCGA GTGTCGTGGC GCGATTTTGC CCGCGCGTGC GGTGTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT
Details for BuxeA.00077.c.B1.PS37868
PURIFICATION DATe: 3/8/2016
CONCENTRATION: 53.8mg/ml
OBSERVED MW: 47kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS VLNETHDPSL KSWVASANAA GSDFPIQNLP FAVFRRNGSP EAFRGGVAIG EMIVDLGAVA RLLDGVVADA ARAGSESSLN ALMAQGSAAW SALRLALSRA LREGALQQAA FANALVAQAD AEYTVPARIG DYTDFYTSIY HATNIGRLFR PDNPLMPNYK WVPIGYHGRA SSIAVSGQQF PRPVGQTFPP GSEQPSFGPS KRLDYELELA VYIGTGNALG SPIPIDDAEA HVFGIGLLND WSARDVQAWE YQPLGPFLAK SFASTVSPWI VTLEALAPYR VAFERNAGDP QPLPYLDSVR QRERGAFDIT LELGIESARL RSQGRPAARV SLTNYRHAYW TIAQMVAHHT VNGCNLQPGD LLGTGTLSGP TLDQAGALIE TTSGGKNPFE LGNGEARTFL EDGDAVIIRG WCEKAGAARI GFGECRGX
Validated NT Sequence
tnnngccacg acactcgccg aagccgatgc gtgccgcacc cgccttctcg caccatccac ggatgatcac cgcatcgccg tcctcgagga aagtccgcgc ctcgccattg ccgagctcga acgggttctt gcctccgctc gtggtctcga tcagcgcgcc cgcctggtcc agcgtcggac ccgacagcgt gcccgtgcca agcaggtcgc ccggctgcag gttgcaaccg ttgaccgtgt gatgcgcgac catctgcgcg atcgtccagt acgcgtggcg gtagttcgtg aggcttacgc gcgcggccgg ccggccctgc gagcgcagcc gcgccgattc gatcccgagc tcgagcgtga tgtcgaatgc gccccgctcg cgctgccgca ccgaatcgag gtaaggcagc ggctgcggat cgcccgcgtt gcgctcgaac gccacgcggt acggagcgag cgcctcgagc gtgacgatcc acggagacac cgtgctcgcg aagctcttcg cgaggaacgg cccgagcggc tgatattccc aagcctggac gtcgcgggcg gaccagtcgt tcaggaggcc gatgccgaac acatgcgcct ccgcatcgtc gatcgggatc ggactgccga gcgcgttgcc agtgccaatg tagacggcga gctcgagctc gtagtcgagc cgcttgctcg gcccgaagct cggctgctcc gagcccggcg gaaacgtctg cccgaccggc cgcgggaact gctggcccga caccgcgatc gacgaggcgc ggccgtgata gccgatcggt acccacttgt agttcggcat cagcggattg tccggccgga acagcctgcc gatattggtc gcatggtaga tcgaggtata gaagtcggtg tagtcgccga tccgcgcggg caccgtgtat tcggcgtcgg cctgcgcgac cagcgcattc gcgaacgcgg cctgctgcag cgcgccctcg cgcagcgcgc gcgatagcgc gagccgtagc gccgaccacg cggccgagcc ctgcgccatc agcgcattga ggctgctctc gctgccggcc cgggccgcgt cggcgaccac accatctagc aggcgcgcga ccgcgccgag atcgacgatc atctcgccga tcgcgacgcc accgcggaac gcctccgggc tgccgttacg gcgaaacacc gcgaacggca agttctggat cggaaaatcg ctaccagccg cgttggccga ggcgacccag ctcttgagac tcggatcgtg ggtttcgttg agaacggaca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMS VLNETHDPSL KSWVASANAA GSDFPIQNLP FAVFRRNGSP EAFRGGVAIG EMIVDLGAVA RLLDGVVADA ARAGSESSLN ALMAQGSAAW SALRLALSRA LREGALQQAA FANALVAQAD AEYTVPARIG DYTDFYTSIY HATNIGRLFR PDNPLMPNYK WVPIGYHGRA SSIAVSGQQF PRPVGQTFPP GSEQPSFGPS KRLDYELELA VYIGTGNALG SPIPIDDAEA HVFGIGLLND WSARDVQAWE YQPLGPFLAK SFASTVSPWI VTLEALAPYR VAFERNAGDP QPLPYLDSVR QRERGAFDIT LELGIESARL RSQGRPAARV SLTNYRHAYW TIAQMVAHHT VNGCNLQPGD LLGTGTLSGP TLDQAGALIE TTSGGKNPFE LGNGEARTFL EDGDAVIIRG WCEKAGAARI GFGECRGAIL PARAV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCG TTCTCAACGA AACCCACGAT CCGAGTCTCA AGAGCTGGGT CGCCTCGGCC AACGCGGCTG GTAGCGATTT TCCGATCCAG AACTTGCCGT TCGCGGTGTT TCGCCGTAAC GGCAGCCCGG AGGCGTTCCG CGGTGGCGTC GCGATCGGCG AGATGATCGT CGATCTCGGC GCGGTCGCGC GCCTGCTAGA TGGTGTGGTC GCCGACGCGG CCCGGGCCGG CAGCGAGAGC AGCCTCAATG CGCTGATGGC GCAGGGCTCG GCCGCGTGGT CGGCGCTACG GCTCGCGCTA TCGCGCGCGC TGCGCGAGGG CGCGCTGCAG CAGGCCGCGT TCGCGAATGC GCTGGTCGCG CAGGCCGACG CCGAATACAC GGTGCCCGCG CGGATCGGCG ACTACACCGA CTTCTATACC TCGATCTACC ATGCGACCAA TATCGGCAGG CTGTTCCGGC CGGACAATCC GCTGATGCCG AACTACAAGT GGGTACCGAT CGGCTATCAC GGCCGCGCCT CGTCGATCGC GGTGTCGGGC CAGCAGTTCC CGCGGCCGGT CGGGCAGACG TTTCCGCCGG GCTCGGAGCA GCCGAGCTTC GGGCCGAGCA AGCGGCTCGA CTACGAGCTC GAGCTCGCCG TCTACATTGG CACTGGCAAC GCGCTCGGCA GTCCGATCCC GATCGACGAT GCGGAGGCGC ATGTGTTCGG CATCGGCCTC CTGAACGACT GGTCCGCCCG CGACGTCCAG GCTTGGGAAT ATCAGCCGCT CGGGCCGTTC CTCGCGAAGA GCTTCGCGAG CACGGTGTCT CCGTGGATCG TCACGCTCGA GGCGCTCGCT CCGTACCGCG TGGCGTTCGA GCGCAACGCG GGCGATCCGC AGCCGCTGCC TTACCTCGAT TCGGTGCGGC AGCGCGAGCG GGGCGCATTC GACATCACGC TCGAGCTCGG GATCGAATCG GCGCGGCTGC GCTCGCAGGG CCGGCCGGCC GCGCGCGTAA GCCTCACGAA CTACCGCCAC GCGTACTGGA CGATCGCGCA GATGGTCGCG CATCACACGG TCAACGGTTG CAACCTGCAG CCGGGCGACC TGCTTGGCAC GGGCACGCTG TCGGGTCCGA CGCTGGACCA GGCGGGCGCG CTGATCGAGA CCACGAGCGG AGGCAAGAAC CCGTTCGAGC TCGGCAATGG CGAGGCGCGG ACTTTCCTCG AGGACGGCGA TGCGGTGATC ATCCGTGGAT GGTGCGAGAA GGCGGGTGCG GCACGCATCG GCTTCGGCGA GTGTCGTGGC GCGATTTTGC CCGCGCGTGC GGTGTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT
Details for BuxeA.00077.c.B1.PS38171
PURIFICATION DATe: 1/18/2017
CONCENTRATION: 32.7mg/ml
OBSERVED MW: 51kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS VLNETHDPSL KSWVASANAA GSDFPIQNLP FAVFRRNGSP EAFRGGVAIG EMIVDLGAVA RLLDGVVADA ARAGSESSLN ALMAQGSAAW SALRLALSRA LREGALQQAA FANALVAQAD AEYTVPARIG DYTDFYTSIY HATNIGRLFR PDNPLMPNYK WVPIGYHGRA SSIAVSGQQF PRPVGQTFPP GSEQPSFGPS KRLDYELELA VYIGTGNALG SPIPIDDAEA HVFGIGLLND WSARDVQAWE YQPLGPFLAK SFASTVSPWI VTLEALAPYR VAFERNAGDP QPLPYLDSVR QRERGAFDIT LELGIESARL RSQGRPAARV SLTNYRHAYW TIAQMVAHHT VNGCNLQPGD LLGTGTLSGP TLDQAGALIE TTSGGKNPFE LGNGEARTFL EDGDAVIIRG WCEKAGAARI GFGECRGX
Validated NT Sequence
tnnngccacg acactcgccg aagccgatgc gtgccgcacc cgccttctcg caccatccac ggatgatcac cgcatcgccg tcctcgagga aagtccgcgc ctcgccattg ccgagctcga acgggttctt gcctccgctc gtggtctcga tcagcgcgcc cgcctggtcc agcgtcggac ccgacagcgt gcccgtgcca agcaggtcgc ccggctgcag gttgcaaccg ttgaccgtgt gatgcgcgac catctgcgcg atcgtccagt acgcgtggcg gtagttcgtg aggcttacgc gcgcggccgg ccggccctgc gagcgcagcc gcgccgattc gatcccgagc tcgagcgtga tgtcgaatgc gccccgctcg cgctgccgca ccgaatcgag gtaaggcagc ggctgcggat cgcccgcgtt gcgctcgaac gccacgcggt acggagcgag cgcctcgagc gtgacgatcc acggagacac cgtgctcgcg aagctcttcg cgaggaacgg cccgagcggc tgatattccc aagcctggac gtcgcgggcg gaccagtcgt tcaggaggcc gatgccgaac acatgcgcct ccgcatcgtc gatcgggatc ggactgccga gcgcgttgcc agtgccaatg tagacggcga gctcgagctc gtagtcgagc cgcttgctcg gcccgaagct cggctgctcc gagcccggcg gaaacgtctg cccgaccggc cgcgggaact gctggcccga caccgcgatc gacgaggcgc ggccgtgata gccgatcggt acccacttgt agttcggcat cagcggattg tccggccgga acagcctgcc gatattggtc gcatggtaga tcgaggtata gaagtcggtg tagtcgccga tccgcgcggg caccgtgtat tcggcgtcgg cctgcgcgac cagcgcattc gcgaacgcgg cctgctgcag cgcgccctcg cgcagcgcgc gcgatagcgc gagccgtagc gccgaccacg cggccgagcc ctgcgccatc agcgcattga ggctgctctc gctgccggcc cgggccgcgt cggcgaccac accatctagc aggcgcgcga ccgcgccgag atcgacgatc atctcgccga tcgcgacgcc accgcggaac gcctccgggc tgccgttacg gcgaaacacc gcgaacggca agttctggat cggaaaatcg ctaccagccg cgttggccga ggcgacccag ctcttgagac tcggatcgtg ggtttcgttg agaacggaca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMS VLNETHDPSL KSWVASANAA GSDFPIQNLP FAVFRRNGSP EAFRGGVAIG EMIVDLGAVA RLLDGVVADA ARAGSESSLN ALMAQGSAAW SALRLALSRA LREGALQQAA FANALVAQAD AEYTVPARIG DYTDFYTSIY HATNIGRLFR PDNPLMPNYK WVPIGYHGRA SSIAVSGQQF PRPVGQTFPP GSEQPSFGPS KRLDYELELA VYIGTGNALG SPIPIDDAEA HVFGIGLLND WSARDVQAWE YQPLGPFLAK SFASTVSPWI VTLEALAPYR VAFERNAGDP QPLPYLDSVR QRERGAFDIT LELGIESARL RSQGRPAARV SLTNYRHAYW TIAQMVAHHT VNGCNLQPGD LLGTGTLSGP TLDQAGALIE TTSGGKNPFE LGNGEARTFL EDGDAVIIRG WCEKAGAARI GFGECRGAIL PARAV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCG TTCTCAACGA AACCCACGAT CCGAGTCTCA AGAGCTGGGT CGCCTCGGCC AACGCGGCTG GTAGCGATTT TCCGATCCAG AACTTGCCGT TCGCGGTGTT TCGCCGTAAC GGCAGCCCGG AGGCGTTCCG CGGTGGCGTC GCGATCGGCG AGATGATCGT CGATCTCGGC GCGGTCGCGC GCCTGCTAGA TGGTGTGGTC GCCGACGCGG CCCGGGCCGG CAGCGAGAGC AGCCTCAATG CGCTGATGGC GCAGGGCTCG GCCGCGTGGT CGGCGCTACG GCTCGCGCTA TCGCGCGCGC TGCGCGAGGG CGCGCTGCAG CAGGCCGCGT TCGCGAATGC GCTGGTCGCG CAGGCCGACG CCGAATACAC GGTGCCCGCG CGGATCGGCG ACTACACCGA CTTCTATACC TCGATCTACC ATGCGACCAA TATCGGCAGG CTGTTCCGGC CGGACAATCC GCTGATGCCG AACTACAAGT GGGTACCGAT CGGCTATCAC GGCCGCGCCT CGTCGATCGC GGTGTCGGGC CAGCAGTTCC CGCGGCCGGT CGGGCAGACG TTTCCGCCGG GCTCGGAGCA GCCGAGCTTC GGGCCGAGCA AGCGGCTCGA CTACGAGCTC GAGCTCGCCG TCTACATTGG CACTGGCAAC GCGCTCGGCA GTCCGATCCC GATCGACGAT GCGGAGGCGC ATGTGTTCGG CATCGGCCTC CTGAACGACT GGTCCGCCCG CGACGTCCAG GCTTGGGAAT ATCAGCCGCT CGGGCCGTTC CTCGCGAAGA GCTTCGCGAG CACGGTGTCT CCGTGGATCG TCACGCTCGA GGCGCTCGCT CCGTACCGCG TGGCGTTCGA GCGCAACGCG GGCGATCCGC AGCCGCTGCC TTACCTCGAT TCGGTGCGGC AGCGCGAGCG GGGCGCATTC GACATCACGC TCGAGCTCGG GATCGAATCG GCGCGGCTGC GCTCGCAGGG CCGGCCGGCC GCGCGCGTAA GCCTCACGAA CTACCGCCAC GCGTACTGGA CGATCGCGCA GATGGTCGCG CATCACACGG TCAACGGTTG CAACCTGCAG CCGGGCGACC TGCTTGGCAC GGGCACGCTG TCGGGTCCGA CGCTGGACCA GGCGGGCGCG CTGATCGAGA CCACGAGCGG AGGCAAGAAC CCGTTCGAGC TCGGCAATGG CGAGGCGCGG ACTTTCCTCG AGGACGGCGA TGCGGTGATC ATCCGTGGAT GGTGCGAGAA GGCGGGTGCG GCACGCATCG GCTTCGGCGA GTGTCGTGGC GCGATTTTGC CCGCGCGTGC GGTGTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT