BuxeA.00068.a

Phenylalanine 4-hydroxylase

CENTER ID: BuxeA.00068.a
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00068.a.B1.GE39494 Full length( BuxeA.00068.a ) 1 336
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.4601
RefSeq: YP_560968.1
UniProt: Q13SM3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHDYCIIMRS NAVFASFPHT RRSTLASPIH TCRHRRARII MPTANTARLK EQFDAGLETR ADFTIDQPTE RYGTVDHAVW QQLYARQTAL LKGRVCDEFL AGVERIGMPA DRVPSFDEIN AKLTPATGWQ IVAVPGLVPD QVFFEHLANR RFPVTWWMRR PDQLDYLQEP DCFHDLFGHV PLLINPVFAD YMHAYGRAAL AANDAGALPL LARLYWYTVE FGLIRDAASP NGVKIYGAGI VSSKGETLYS QQSAAPNRLG FDLERVMRTR YRIDTFQKTY FVIDDFAQLF NVTQTDFTPL LARLAAEPAF AAGDVLEHDR VMTRGSREGW LADGDI
NT Sequence
ATGCACGATT ATTGCATAAT CATGCGCTCA AACGCCGTCT TTGCGTCCTT TCCGCATACG CGACGCTCTA CACTTGCATC ACCGATTCAC ACTTGCCGTC ATCGTAGAGC GCGCATCATC ATGCCCACCG CCAATACCGC CAGACTGAAA GAGCAATTCG ACGCCGGACT CGAAACCCGC GCCGATTTCA CCATCGACCA ACCAACCGAA CGCTACGGCA CGGTCGACCA CGCGGTATGG CAGCAGCTCT ATGCGCGCCA GACAGCGCTG CTCAAAGGGC GCGTCTGTGA CGAGTTTCTC GCGGGAGTCG AACGCATCGG CATGCCGGCC GACCGCGTGC CCTCCTTCGA CGAGATCAAT GCGAAGCTCA CGCCCGCCAC CGGCTGGCAG ATAGTCGCCG TGCCCGGACT CGTGCCGGAC CAGGTGTTCT TCGAGCATCT GGCGAATCGC CGCTTTCCCG TCACATGGTG GATGCGCCGG CCGGATCAAC TCGATTACTT GCAGGAGCCG GATTGTTTTC ATGATCTGTT CGGCCACGTG CCGCTGCTGA TCAACCCGGT TTTCGCCGAC TACATGCACG CGTATGGCCG CGCCGCGCTC GCCGCCAACG ACGCCGGTGC GCTGCCGCTG CTCGCGCGGC TCTACTGGTA CACCGTGGAG TTCGGCCTGA TCCGCGACGC GGCGAGTCCG AACGGCGTGA AGATCTATGG CGCGGGAATC GTGTCGAGCA AGGGCGAAAC GCTCTACAGC CAGCAAAGCG CCGCGCCGAA CCGCCTTGGC TTCGACCTCG AACGCGTGAT GCGCACGCGC TACCGTATCG ATACGTTCCA GAAGACTTAC TTCGTGATCG ACGACTTCGC GCAGTTGTTC AACGTGACGC AAACCGATTT CACGCCGCTG CTGGCAAGGC TGGCAGCCGA ACCCGCATTC GCGGCCGGCG ACGTCCTCGA ACACGATCGC GTGATGACAC GCGGCTCGCG CGAAGGCTGG CTGGCAGACG GCGATATC
Details for BuxeA.00068.a.B1.GE39494
HARVESTED ON: 9/10/2015
SEQUENCED ON: 9/16/2015
EXPECTED MW: 39kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMH DYCIIMRSNA VFASFPHTRR STLASPIHTC RHRRARIIXP TANTARLKEQ FDAGLETRAD FTIDQPTERY GTVDHAVWQQ LYARQTALLK GRVCDEFLAG VERIGMPADR VPSFDEINAK LTPATGWQIV AVPGLVPDQV FFEHLANRRF PVTWWMRRPD QLDYLQEPDC FHDLFGHVPL LINPVFADYM HAYGRAALAA NDAGALPLLA RLYWYTVEFG LIRDAASPNG VKIYGAGIVS SKGETLYSQQ SAXPNRLGFD LERVMRTRYR IDTFQKTYFV IDDFAQLFXX XQTDFTPLLA RLAAEPAFAA GDVLEHDRVM TRGSR
Validated NT Sequence
ttcgcgcgag ccgcgtgtca tcacgcgatc gtgttcgagg acgtcgccgg ccgcgaatgc gggttcggct gccagccttg ccagcagcgg cgtgaaatcg gtttgcgnnn nnntgaacaa ctgcgcgaag tcgtcgatca cgaagtaagt cttctggaac gtatcgatac ggtagcgcgt gcgcatcacg cgttcgaggt cgaagccaag gcggttcggc nnngcgcttt gctggctgta gagcgtttcg cccttgctcg acacgattcc cgcgccatag atcttcacgc cgttcggact cgccgcgtcg cggatcaggc cgaactccac ggtgtaccag tagagccgcg cgagcagcgg cagcgcaccg gcgtcgttgg cggcgagcgc ggcgcggcca tacgcgtgca tgtagtcggc gaaaaccggg ttgatcagca gcggcacgtg gccgaacaga tcatgaaaac aatccggctc ctgcaagtaa tcgagttgat ccggccggcg catccaccat gtgacgggaa agcggcgatt cgccagatgc tcgaagaaca cctggtccgg cacgagtccg ggcacggcga ctatctgcca gccggtggcg ggcgtgagct tcgcattgat ctcgtcgaag gagggcacgc ggtcggccgg catgccgatg cgttcgactc ccgcgagaaa ctcgtcacag acgcgccctt tgagcagcgc tgtctggcgc gcatagagct gctgccatac cgcgtggtcg accgtgccgt agcgttcggt tggttggtcg atggtgaaat cggcgcgggt ttcgagtccg gcgtcgaatt gctctttcag tctggcggta ttggcggtgg gnatgatgat gcgcgctcta cgatgacggc aagtgtgaat cggtgatgca agtgtagagc gtcgcgtatg cggaaaggac gcaaagacgg cgtttgagcg catgattatg caataatcgt gcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMH DYCIIMRSNA VFASFPHTRR STLASPIHTC RHRRARIIMP TANTARLKEQ FDAGLETRAD FTIDQPTERY GTVDHAVWQQ LYARQTALLK GRVCDEFLAG VERIGMPADR VPSFDEINAK LTPATGWQIV AVPGLVPDQV FFEHLANRRF PVTWWMRRPD QLDYLQEPDC FHDLFGHVPL LINPVFADYM HAYGRAALAA NDAGALPLLA RLYWYTVEFG LIRDAASPNG VKIYGAGIVS SKGETLYSQQ SAAPNRLGFD LERVMRTRYR IDTFQKTYFV IDDFAQLFNV TQTDFTPLLA RLAAEPAFAA GDVLEHDRVM TRGSREGWLA DGDI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcacg attattgcat aatcatgcgc tcaaacgccg tctttgcgtc ctttccgcat acgcgacgct ctacacttgc atcaccgatt cacacttgcc gtcatcgtag agcgcgcatc atcatgccca ccgccaatac cgccagactg aaagagcaat tcgacgccgg actcgaaacc cgcgccgatt tcaccatcga ccaaccaacc gaacgctacg gcacggtcga ccacgcggta tggcagcagc tctatgcgcg ccagacagcg ctgctcaaag ggcgcgtctg tgacgagttt ctcgcgggag tcgaacgcat cggcatgccg gccgaccgcg tgccctcctt cgacgagatc aatgcgaagc tcacgcccgc caccggctgg cagatagtcg ccgtgcccgg actcgtgccg gaccaggtgt tcttcgagca tctggcgaat cgccgctttc ccgtcacatg gtggatgcgc cggccggatc aactcgatta cttgcaggag ccggattgtt ttcatgatct gttcggccac gtgccgctgc tgatcaaccc ggttttcgcc gactacatgc acgcgtatgg ccgcgccgcg ctcgccgcca acgacgccgg tgcgctgccg ctgctcgcgc ggctctactg gtacaccgtg gagttcggcc tgatccgcga cgcggcgagt ccgaacggcg tgaagatcta tggcgcggga atcgtgtcga gcaagggcga aacgctctac agccagcaaa gcgccgcgcc gaaccgcctt ggcttcgacc tcgaacgcgt gatgcgcacg cgctaccgta tcgatacgtt ccagaagact tacttcgtga tcgacgactt cgcgcagttg ttcaacgtga cgcaaaccga tttcacgccg ctgctggcaa ggctggcagc cgaacccgca ttcgcggccg gcgacgtcct cgaacacgat cgcgtgatga cacgcggctc gcgcgaaggc tggctggcag acggcgatat ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat