SSGCID: Target Page

BuxeA.00065.a

4-hydroxybenzoate 3-monooxygenase (PobB)

CENTER ID:	BuxeA.00065.a
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00065.a.B1.GE39493	Full length( BuxeA.00065.a )		1	394

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00065.a.B1.PS37837	Full length( BuxeA.00065.a )		1	394

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.2502
RefSeq:	YP_558978.1
UniProt:	Q13YB3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRTQVGIVGA GPAGLLLSHL LHLRGIDSVV LESRARDQIE STIRAGVLEQ GTMDLLTDAG VGARMKAEGA LHHGFELAFE GKRRRIDLTG LTGHSITVYA QHEVIKDLVA ARVADGGKLR FGVSQTSIHD IETDKPSIRY HHEGEAYELQ CDFVIGCDGS QGVSRASIPP ALRRDFERVY PFGWFGILCE GPPSSDELIY ARHERGFALV STRSPNVQRM YFQCDPKDSV ENWSDDRIWA ELHARVDSHD GQKIIDGKIF QKNIVGMRSF VSNTMQHGRL FLAGDAAHIV PPTGAKGMNL AVADVRVLTE ALNAFYVENR TDLLDGYSAT ALKRIWRAEH FSYWMTSMMH RIDGASPFDQ QLQVAELEYV TTSRAAATAM AENYVGVAGA QAQA

NT Sequence

ATGCGCACTC AAGTTGGCAT CGTCGGTGCC GGGCCTGCGG GTCTGCTGCT TTCCCATCTT CTTCACCTGC GTGGCATCGA CTCCGTCGTG CTCGAGTCGC GCGCTCGTGA TCAGATCGAA TCCACCATTC GCGCGGGCGT ACTCGAACAG GGCACGATGG ACCTGTTGAC CGATGCCGGC GTCGGCGCGC GGATGAAAGC CGAAGGCGCG CTGCATCACG GCTTCGAACT GGCTTTCGAA GGCAAGCGGC GCCGCATCGA TCTGACTGGA CTCACGGGGC ATTCCATTAC GGTCTACGCA CAGCACGAAG TGATCAAGGA CCTCGTCGCG GCGCGTGTGG CCGACGGCGG AAAGCTGCGT TTCGGCGTGT CGCAGACCTC GATTCACGAC ATCGAAACGG ACAAGCCGTC GATCCGCTAT CACCACGAAG GCGAAGCGTA CGAACTGCAA TGCGACTTCG TGATCGGCTG CGACGGTTCG CAAGGCGTGT CGCGCGCATC GATTCCACCG GCATTGCGCA GGGATTTCGA ACGTGTGTAC CCATTCGGTT GGTTCGGAAT CCTGTGTGAA GGGCCGCCGT CGTCGGATGA ACTGATCTAC GCGCGTCACG AGCGCGGTTT CGCGCTGGTC AGCACGCGCT CGCCGAACGT GCAGCGCATG TACTTCCAGT GCGATCCTAA AGATTCGGTC GAGAACTGGT CCGACGACCG GATCTGGGCG GAACTGCATG CGCGAGTCGA CTCGCACGAC GGACAGAAAA TTATCGACGG CAAGATCTTC CAGAAGAACA TTGTCGGCAT GCGCAGTTTC GTGTCGAACA CCATGCAGCA TGGCCGGCTA TTTCTGGCCG GCGATGCGGC GCATATCGTG CCGCCCACCG GCGCGAAAGG CATGAACCTG GCCGTGGCCG ACGTGCGTGT GCTGACAGAG GCGCTCAATG CGTTCTACGT TGAAAACCGC ACGGACCTGC TGGACGGCTA CAGCGCGACC GCGCTTAAAC GCATCTGGCG TGCCGAGCAC TTCTCGTACT GGATGACCAG CATGATGCAC CGTATCGACG GTGCATCGCC TTTCGACCAG CAGTTGCAGG TCGCCGAGCT CGAATATGTG ACCACCTCGC GCGCGGCGGC CACCGCGATG GCCGAAAATT ACGTTGGCGT GGCCGGCGCG CAAGCGCAAG CA

Details for BuxeA.00065.a.B1.GE39493

HARVESTED ON:	9/10/2015
SEQUENCED ON:	9/16/2015
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	77
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHXXX RTQVGIVXXG PAGLLLSHLL HLRXXXXXVL XXXXXXQIES TIXAGVLXQG TMDLLTDAXX XARMXXXXAL XXXXXLAXXX KRRRXXLTGL TGHSITVXAQ XXVIKDLVXX RVXDGGKLRF GVSQXSIHDI ETDXXXXRYH HXGEAXXLQC DFVIGCDGXK GXSRASIPXX LRXDFERVYP FGWFGILCEG PPSSDELIXX RHERGFALVS TRSXNVQRMY FXXXXXDSVE XXSDDRIWAE LHARVDSHDG QKIIDGKIFQ KNIVGMRSXV SNTMQHXXLF LAGDAXHIVP PTGAKGMNXX VADVXXLTXX XNAXXVENRT DLXDGYSATA LKRXXRAEHF SYWXTSMMXX IDGASPFXQQ LQVAXXXYVT TSRAAATAMA ENY

Validated NT Sequence

cgtaattttc ggccatcgcg gtggccgccg cgcgcgaggt ggtcacatan nnnnnntcgg cnacctgcaa ctgctgntcg aaaggcgatg caccgtcgat acnntgcatc atgctggtnn nccagtacga gaagtgctcg gcacgnnnnn tgcgtttaag cgcggtcgcg ctgtagccgt cnnncaggtc cgtgcggttt tcaacgtnnn acgcattgan nnnntctgtc agcannnnca cgtcggccac gnnnnngttc atgcctttcg cgccggtggg cggcacgata tgcnncgcat cgccggccag aaatagnngn nnatgctgca tggtgttcga cacnnaactg cgcatgccga caatgttctt ctggaagatc ttgccgtcga taattttctg tccgtcgtgc gagtcgactc gcgcatgcag ttccgcccag atccggtcgt cggaccnnnt ctcgaccgaa tcttnannan cgcnnnggaa gtacatgcgc tgcacgttnn nngagcgcgt gctgaccagc gcgaaaccgc gctcgtgacg nnnntagatc agttcatccg acgacggcgg cccttcacac aggattccga accaaccgaa tgggtacaca cgttcgaaat ccangcgcaa tgncngtgga atcgatgcgc gcgacncgcc tttnnaaccg tcgcagccga tcacgaagtc gcattgcagn nnnnacgctt cgccttngtg gtgatagcgg annnannnnn ngtccgtttc gatgtcgtga atcgagntct gcgacacgcc gaancgcagc tttccgccgt cnnncacacg cgnnnnnacg aggtccttga tcactnnnnn ctgtgcntag accgtaatgg aatgccccgt gagtccagtc agannnatgc ggcgccgctt nnnnnnnnan gccagnnnnn nnnnntgann nagcgcgnnn nnnnnnttca tccgcgcgcn nannnnggca tcggtcaaca ggtccatcgt gccctgttnn agtacgcccg cnnnaatggt ggattcgatc tgnnnnnnnn nnnncnacnn nagcacnncg gnnncnannn nncgcaggtg aagaagatgg gaaagcagca gacccgcagg cccnnnnncg acgatgccaa cttgagtgcg cannnnnngg tggtggtggt ggtgagccat

Expected Protein Sequence

MAHHHHHHMR TQVGIVGAGP AGLLLSHLLH LRGIDSVVLE SRARDQIEST IRAGVLEQGT MDLLTDAGVG ARMKAEGALH HGFELAFEGK RRRIDLTGLT GHSITVYAQH EVIKDLVAAR VADGGKLRFG VSQTSIHDIE TDKPSIRYHH EGEAYELQCD FVIGCDGSQG VSRASIPPAL RRDFERVYPF GWFGILCEGP PSSDELIYAR HERGFALVST RSPNVQRMYF QCDPKDSVEN WSDDRIWAEL HARVDSHDGQ KIIDGKIFQK NIVGMRSFVS NTMQHGRLFL AGDAAHIVPP TGAKGMNLAV ADVRVLTEAL NAFYVENRTD LLDGYSATAL KRIWRAEHFS YWMTSMMHRI DGASPFDQQL QVAELEYVTT SRAAATAMAE NYVGVAGAQA QA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgca ctcaagttgg catcgtcggt gccgggcctg cgggtctgct gctttcccat cttcttcacc tgcgtggcat cgactccgtc gtgctcgagt cgcgcgctcg tgatcagatc gaatccacca ttcgcgcggg cgtactcgaa cagggcacga tggacctgtt gaccgatgcc ggcgtcggcg cgcggatgaa agccgaaggc gcgctgcatc acggcttcga actggctttc gaaggcaagc ggcgccgcat cgatctgact ggactcacgg ggcattccat tacggtctac gcacagcacg aagtgatcaa ggacctcgtc gcggcgcgtg tggccgacgg cggaaagctg cgtttcggcg tgtcgcagac ctcgattcac gacatcgaaa cggacaagcc gtcgatccgc tatcaccacg aaggcgaagc gtacgaactg caatgcgact tcgtgatcgg ctgcgacggt tcgcaaggcg tgtcgcgcgc atcgattcca ccggcattgc gcagggattt cgaacgtgtg tacccattcg gttggttcgg aatcctgtgt gaagggccgc cgtcgtcgga tgaactgatc tacgcgcgtc acgagcgcgg tttcgcgctg gtcagcacgc gctcgccgaa cgtgcagcgc atgtacttcc agtgcgatcc taaagattcg gtcgagaact ggtccgacga ccggatctgg gcggaactgc atgcgcgagt cgactcgcac gacggacaga aaattatcga cggcaagatc ttccagaaga acattgtcgg catgcgcagt ttcgtgtcga acaccatgca gcatggccgg ctatttctgg ccggcgatgc ggcgcatatc gtgccgccca ccggcgcgaa aggcatgaac ctggccgtgg ccgacgtgcg tgtgctgaca gaggcgctca atgcgttcta cgttgaaaac cgcacggacc tgctggacgg ctacagcgcg accgcgctta aacgcatctg gcgtgccgag cacttctcgt actggatgac cagcatgatg caccgtatcg acggtgcatc gcctttcgac cagcagttgc aggtcgccga gctcgaatat gtgaccacct cgcgcgcggc ggccaccgcg atggccgaaa attacgttgg cgtggccggc gcgcaagcgc aagcatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for BuxeA.00065.a.B1.PS37837

PURIFICATION DATe:	2/16/2016
CONCENTRATION:	11.7mg/ml
OBSERVED MW:	45kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	SEC
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	77
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGCA CTCAAGTTGG CATCGTCGGT GCCGGGCCTG CGGGTCTGCT GCTTTCCCAT CTTCTTCACC TGCGTGGCAT CGACTCCGTC GTGCTCGAGT CGCGCGCTCG TGATCAGATC GAATCCACCA TTCGCGCGGG CGTACTCGAA CAGGGCACGA TGGACCTGTT GACCGATGCC GGCGTCGGCG CGCGGATGAA AGCCGAAGGC GCGCTGCATC ACGGCTTCGA ACTGGCTTTC GAAGGCAAGC GGCGCCGCAT CGATCTGACT GGACTCACGG GGCATTCCAT TACGGTCTAC GCACAGCACG AAGTGATCAA GGACCTCGTC GCGGCGCGTG TGGCCGACGG CGGAAAGCTG CGTTTCGGCG TGTCGCAGAC CTCGATTCAC GACATCGAAA CGGACAAGCC GTCGATCCGC TATCACCACG AAGGCGAAGC GTACGAACTG CAATGCGACT TCGTGATCGG CTGCGACGGT TCGCAAGGCG TGTCGCGCGC ATCGATTCCA CCGGCATTGC GCAGGGATTT CGAACGTGTG TACCCATTCG GTTGGTTCGG AATCCTGTGT GAAGGGCCGC CGTCGTCGGA TGAACTGATC TACGCGCGTC ACGAGCGCGG TTTCGCGCTG GTCAGCACGC GCTCGCCGAA CGTGCAGCGC ATGTACTTCC AGTGCGATCC TAAAGATTCG GTCGAGAACT GGTCCGACGA CCGGATCTGG GCGGAACTGC ATGCGCGAGT CGACTCGCAC GACGGACAGA AAATTATCGA CGGCAAGATC TTCCAGAAGA ACATTGTCGG CATGCGCAGT TTCGTGTCGA ACACCATGCA GCATGGCCGG CTATTTCTGG CCGGCGATGC GGCGCATATC GTGCCGCCCA CCGGCGCGAA AGGCATGAAC CTGGCCGTGG CCGACGTGCG TGTGCTGACA GAGGCGCTCA ATGCGTTCTA CGTTGAAAAC CGCACGGACC TGCTGGACGG CTACAGCGCG ACCGCGCTTA AACGCATCTG GCGTGCCGAG CACTTCTCGT ACTGGATGAC CAGCATGATG CACCGTATCG ACGGTGCATC GCCTTTCGAC CAGCAGTTGC AGGTCGCCGA GCTCGAATAT GTGACCACCT CGCGCGCGGC GGCCACCGCG ATGGCCGAAA ATTACGTTGG CGTGGCCGGC GCGCAAGCGC AAGCATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT