SSGCID: Target Page

BuxeA.00057.w

ABC sugar transporter, ATPase subunit

CENTER ID:	BuxeA.00057.w
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00057.w.A1.GE33010	Full length( BuxeA.00057.w )		1	265

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.6886
RefSeq:	YP_554412.1
UniProt:	Q13LH7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTPTSASTA MPVLQARGLV KRYGNVTALD GCDFEVLPGE ILAVIGDNGA GKSSLIKALS GATVPDEGEI LLDGKPVRFR SPLDARAQGI ETVYQELAVA PAMSIAENLF LARELVKPGW RGSIFRMIDK RRMLEEATAH MKDLQIGIRS MRQAVETLSG GQRQGVAVAR SAAFARHVVI LDEPTAALGV KEGNMVLELI RRVRDRGLPV ILISHNMPHV FEVADRIHIQ RLGRRAALVN TKDVHMSEAV AIMTGAKEAD VKAIA

NT Sequence

ATGTCGACAC CCACTTCCGC TTCGACCGCC ATGCCCGTTC TACAGGCGCG CGGACTCGTC AAACGTTATG GCAACGTGAC CGCGCTCGAC GGTTGCGATT TCGAAGTGCT GCCCGGCGAG ATTCTCGCCG TGATCGGCGA TAACGGCGCG GGGAAATCTT CGCTGATCAA GGCCCTTTCC GGCGCCACGG TGCCGGATGA AGGCGAGATT CTGCTCGACG GCAAACCGGT CAGGTTCCGC AGCCCGCTCG ATGCCCGCGC GCAAGGCATC GAAACGGTGT ATCAGGAACT CGCGGTCGCG CCGGCCATGA GCATCGCCGA AAATCTGTTC CTCGCCCGCG AGCTGGTGAA ACCGGGCTGG CGCGGTTCGA TCTTCAGGAT GATCGACAAG CGCCGCATGC TGGAGGAAGC AACCGCGCAT ATGAAGGATC TGCAGATCGG CATCCGTTCG ATGCGCCAGG CCGTCGAAAC GCTCTCGGGC GGCCAGCGCC AGGGCGTGGC TGTGGCGCGC AGCGCGGCTT TCGCGCGGCA CGTGGTGATT CTCGACGAAC CCACCGCCGC GCTCGGCGTG AAGGAAGGCA ACATGGTGCT GGAACTGATC CGGCGCGTGC GTGATCGCGG CCTACCGGTG ATCCTCATCA GCCACAACAT GCCGCACGTG TTCGAGGTCG CGGACCGCAT CCATATCCAG CGGCTCGGCC GGCGTGCCGC GCTCGTCAAC ACTAAGGACG TGCATATGTC GGAAGCCGTG GCGATCATGA CGGGCGCGAA GGAAGCCGAC GTCAAGGCGA TTGCA

Details for BuxeA.00057.w.A1.GE33010

HARVESTED ON:	8/16/2011
SEQUENCED ON:	8/22/2011
EXPECTED MW:	30kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSTPTSAST AMPVLQARGL VKRYGNVTAL DGCDFEVLPG EILAVIGDNG AGKSSLIKAL SGATVPDEGE ILLDGKPVRF RSPLDARAQG IETVYQELAV APAMSIAENL FLARELVKPG WRGSIFRMID KRRMLEEATA HMKDLQIGIR SMRQAVETLS GGQRQGVAVA RSAAFARHVV ILDEPTAALG VKEGNMVLEL IRRVRDRGLP VILISHNMPH VFEVADRIHI QRLGRRAALV NTKDVHMSEA VAIMTGAKEA DVKAIA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgacaccc acttccgctt cgaccgccat gcccgttcta caggcgcgcg gactcgtcaa acgttatggc aacgtgaccg cgctcgacgg ttgcgatttc gaagtgctgc ccggcgagat tctcgccgtg atcggcgata acggcgcggg gaaatcttcg ctgatcaagg ccctttccgg cgccacggtg ccggatgaag gcgagattct gctcgacggc aaaccggtca ggttccgcag cccgctcgat gcccgcgcgc aaggcatcga aacggtgtat caggaactcg cggtcgcgcc ggccatgagc atcgccgaaa atctgttcct cgcccgcgag ctggtgaaac cgggctggcg cggttcgatc ttcaggatga tcgacaagcg ccgcatgctg gaggaagcaa ccgcgcatat gaaggatctg cagatcggca tccgttcgat gcgccaggcc gtcgaaacgc tctcgggcgg ccagcgccag ggcgtggctg tggcgcgcag cgcggctttc gcgcggcacg tggtgattct cgacgaaccc accgccgcgc tcggcgtgaa ggaaggcaac atggtgctgg aactgatccg gcgcgtgcgt gatcgcggcc taccggtgat cctcatcagc cacaacatgc cgcacgtgtt cgaggtcgcg gaccgcatcc atatccagcg gctcggccgg cgtgccgcgc tcgtcaacac taaggacgtg catatgtcgg aagccgtggc gatcatgacg ggcgcgaagg aagccgacgt caaggcgatt gcataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa