BuxeA.00057.t

ABC sugar transporter, ATPase subunit

CENTER ID: BuxeA.00057.t
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00057.t.A1.GE33007 Full length( BuxeA.00057.t ) 1 365
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.7704
RefSeq: YP_555201.1
UniProt: Q13J88

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAKIEFHNVG KRYINGHVAL ENLSLQIRDK EFLVLLGPSG CGKSTTLNMI AGLEEVTDGS LRFDDVEVNS VPAHKRDVAM VFQSYALYPH KNVYENIAFG LRMRRHPQAE IDSRVRAAAR SLDIEHLLER RPGQLSGGQR QRVALGRAMV RQPAAFLMDE PLSNLDAALR VSMRASIKAL HQSMQTTFVY VTHDQAEALT LADRIVVMRD GVVQQIGSPD DIYERPANKF VANFLGSPPI NFIDGVLQDD GNGLRFRRGD LSLALDDSTR RRLSGRAGQS VSLGIRAEDV TEDAEDVECR LVGQITALLP AGSDQFICVQ GEGQPMFFRV GKERSLRIGE RISITVNRAR MHVFDQAEGT NLKLV
NT Sequence
ATGGCAAAAA TCGAATTCCA TAATGTCGGA AAGCGTTACA TAAACGGGCA CGTCGCACTT GAAAACCTGA GCCTGCAGAT CCGCGACAAG GAGTTCCTTG TGTTGCTGGG ACCTTCTGGT TGTGGGAAGT CCACGACCCT GAACATGATC GCCGGGCTTG AGGAGGTCAC CGACGGTAGC CTGCGCTTTG ACGACGTCGA GGTCAATAGC GTGCCAGCCC ATAAGCGGGA CGTGGCGATG GTGTTCCAGA GCTACGCGCT CTATCCGCAC AAGAACGTCT ACGAAAATAT CGCCTTCGGT CTGCGGATGC GCAGACACCC ACAAGCGGAA ATCGACTCAC GCGTACGCGC AGCCGCCCGT TCGCTTGATA TCGAGCATCT GCTTGAGCGG CGCCCCGGCC AGCTTTCTGG CGGACAGCGT CAGCGGGTAG CGCTCGGGAG GGCGATGGTG CGCCAGCCCG CCGCGTTCCT GATGGACGAG CCGCTGTCGA ACCTGGACGC GGCGCTGCGC GTGTCAATGC GTGCGAGCAT CAAGGCGCTA CACCAGTCCA TGCAGACGAC GTTTGTCTAC GTAACACACG ACCAGGCCGA GGCGCTGACA CTCGCCGACC GGATCGTTGT AATGCGTGAC GGGGTCGTGC AGCAGATCGG CTCCCCGGAT GATATCTACG AGCGACCAGC AAACAAGTTT GTCGCGAATT TTCTCGGTAG CCCGCCGATC AACTTCATCG ATGGCGTTCT TCAGGACGAC GGTAACGGCT TGCGCTTCCG GCGCGGCGAC CTGTCACTCG CGTTGGATGA CTCGACGCGA CGCCGACTCT CGGGCCGTGC GGGACAGAGC GTATCTCTCG GCATTCGCGC CGAGGACGTC ACTGAAGATG CGGAGGATGT CGAGTGTCGT CTCGTCGGGC AGATCACGGC ACTGCTCCCG GCGGGCTCCG ATCAGTTCAT CTGCGTGCAG GGGGAAGGAC AGCCAATGTT CTTCCGTGTC GGTAAGGAGC GCTCGCTGCG GATCGGCGAG CGCATCTCGA TCACGGTCAA TCGCGCACGC ATGCATGTGT TCGATCAGGC CGAAGGCACT AACCTTAAGC TTGTA
Details for BuxeA.00057.t.A1.GE33007
HARVESTED ON: 8/16/2011
SEQUENCED ON: 8/22/2011
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 74
PERCENT COVERAGE: 106
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAKIEFHNV GKRYINGHVA LENLSLQIRD KEFLVLLGPS GCGKSTTLNM IAGLEEVTDG SLRFDDVEVN SVPAHKRDVA MVFQSYALYP HKNVYENIAF GLRMRRHPQA EIDSRVRAAA RSLDIEHLLE RRPGQLSGGQ RQRVALGRAM VRQPAAFLMD EPLSNLDAAL RVSMRASIKA LHQSMQTTFV YVTHDQAEAL TLADRIVVMR DGVVQQIGSP DDIYERPANK FVANFLGSPP INFIDGVLQD DGXACASGAA XCHSRWMTRR DAXSRAVRDR AXLSAFAPRT SLXXRRXSSX VSSGXSRHCX RRAXISSSAX XGKDSXXLXX XXXXXXXSXX XXXXXVXXXX XXXXXXXXXX TXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XX
Validated NT Sequence
ttttgtnnnn tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggcaaa aatcgaattc cataatgtcg gaaagcgtta cataaacggg cacgtcgcac ttgaaaacct gagcctgcag atccgcgaca aggagttcct tgtgttgctg ggaccttctg gttgtgggaa gtccacgacc ctgaacatga tcgccgggct tgaggaggtc accgacggta gcctgcgctt tgacgacgtc gaggtcaata gcgtgccagc ccataagcgg gacgtggcga tggtgttcca gagctacgcg ctctatccgc acaagaacgt ctacgaaaat atcgccttcg gtctgcggat gcgcagacac ccacaagcgg aaatcgactc acgcgtacgc gcagccgccc gttcgcttga tatcgagcat ctgcttgagc ggcgccccgg ccagctttct ggcggacagc gtcagcgggt agcgctcggg agggcgatgg tgcgccagcc cgccgcgttc ctgatggacg agccgctgtc gaacctggac gcggcgctgc gcgtgtcaat gcgtgcgagc atcaaggcgc tacaccagtc catgcagacg acgtttgtct acgtaacaca cgaccaggcc gaggcgctga cactcgccga ccggatcgtt gtaatgcgtg acggggtcgt gcagcagatc ggctccccgg atgatatcta cgagcgacca gcaaacaagt ttgtcgcgaa ttttctcggt agcccgccga tcaacttcat cgatggcgtt cttcaggacg acggnncggc ttgcgcttcc ggngcggcga nctgtcactc gcgttggatg actcgacgcg acgccnactc tcgggccgtg cgggacagag cgnatctctc ggcattcgcg ccgaggacgt cnctgaanan gcggaggang tcgagtgncg tctcgtcggg canatcacgg cactgctncc ggcgggctnc gatcagttca tctgcgtgnn nggggaagga cagcnangnt cttncnngta annngngntn nnnnnncnna tcgnnnannn nannnncgat nnnggtcaan nnnnnnnnnn nnnnntnnnn nnnnnnngnc nannnnnnna cccnanctng nnnnnnnann nnnnnaanaa ntnnnnnnnc nnannttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAKIEFHNV GKRYINGHVA LENLSLQIRD KEFLVLLGPS GCGKSTTLNM IAGLEEVTDG SLRFDDVEVN SVPAHKRDVA MVFQSYALYP HKNVYENIAF GLRMRRHPQA EIDSRVRAAA RSLDIEHLLE RRPGQLSGGQ RQRVALGRAM VRQPAAFLMD EPLSNLDAAL RVSMRASIKA LHQSMQTTFV YVTHDQAEAL TLADRIVVMR DGVVQQIGSP DDIYERPANK FVANFLGSPP INFIDGVLQD DGNGLRFRRG DLSLALDDST RRRLSGRAGQ SVSLGIRAED VTEDAEDVEC RLVGQITALL PAGSDQFICV QGEGQPMFFR VGKERSLRIG ERISITVNRA RMHVFDQAEG TNLKLV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcaaaaatc gaattccata atgtcggaaa gcgttacata aacgggcacg tcgcacttga aaacctgagc ctgcagatcc gcgacaagga gttccttgtg ttgctgggac cttctggttg tgggaagtcc acgaccctga acatgatcgc cgggcttgag gaggtcaccg acggtagcct gcgctttgac gacgtcgagg tcaatagcgt gccagcccat aagcgggacg tggcgatggt gttccagagc tacgcgctct atccgcacaa gaacgtctac gaaaatatcg ccttcggtct gcggatgcgc agacacccac aagcggaaat cgactcacgc gtacgcgcag ccgcccgttc gcttgatatc gagcatctgc ttgagcggcg ccccggccag ctttctggcg gacagcgtca gcgggtagcg ctcgggaggg cgatggtgcg ccagcccgcc gcgttcctga tggacgagcc gctgtcgaac ctggacgcgg cgctgcgcgt gtcaatgcgt gcgagcatca aggcgctaca ccagtccatg cagacgacgt ttgtctacgt aacacacgac caggccgagg cgctgacact cgccgaccgg atcgttgtaa tgcgtgacgg ggtcgtgcag cagatcggct ccccggatga tatctacgag cgaccagcaa acaagtttgt cgcgaatttt ctcggtagcc cgccgatcaa cttcatcgat ggcgttcttc aggacgacgg taacggcttg cgcttccggc gcggcgacct gtcactcgcg ttggatgact cgacgcgacg ccgactctcg ggccgtgcgg gacagagcgt atctctcggc attcgcgccg aggacgtcac tgaagatgcg gaggatgtcg agtgtcgtct cgtcgggcag atcacggcac tgctcccggc gggctccgat cagttcatct gcgtgcaggg ggaaggacag ccaatgttct tccgtgtcgg taaggagcgc tcgctgcgga tcggcgagcg catctcgatc acggtcaatc gcgcacgcat gcatgtgttc gatcaggccg aaggcactaa ccttaagctt gtataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa