BuxeA.00057.s

ABC branched chain amino acid family transporter, ATPase subunit

CENTER ID: BuxeA.00057.s
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00057.s.A1.GE33006 Full length( BuxeA.00057.s ) 1 302
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.7877
RefSeq: YP_555373.1
UniProt: Q13IR6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNPASVSGRD ILFDVRRVTR RFDGLTAVDG VSLSVARGEC VSVIGPNGAG KSTLFNLLTG MDVPDEGEIR LDGEDVTGTS PERLAARGVA RTFQHGRVFG NLSVLDNVLI GAHSRLALAR PGWPVLGAFS EVVRALVRPS ALRREEAALR ADAEAIIARF GTRLTPRIEQ PAHSLSYANR RRVEIARALA LRPRMLLLDE PTAGMNESET AEMLELILDL KRDGLTILLI EHKLDLVMRL SDRVIVLDDG RKIAAGLPDA VRNDPAVIDA YLGHRHVGAQ AGPAAQAVAA RSDSTVTGAQ HA
NT Sequence
ATGAACCCTG CATCGGTAAG CGGCCGGGAC ATCCTGTTCG ACGTGCGGCG CGTGACGCGC CGCTTCGACG GCCTGACCGC GGTGGATGGC GTGAGCCTGT CAGTGGCGCG CGGCGAATGC GTGAGCGTGA TCGGCCCGAA CGGCGCGGGC AAGTCGACGC TGTTCAATCT GCTGACCGGC ATGGATGTGC CGGACGAAGG CGAGATTCGC CTCGACGGTG AGGACGTGAC CGGTACTTCG CCGGAACGCC TCGCCGCGCG CGGCGTAGCG CGCACGTTTC AGCACGGCCG CGTGTTCGGC AACCTGAGCG TGCTCGATAA CGTGTTGATC GGCGCGCATT CCCGGCTTGC GCTGGCGCGT CCAGGCTGGC CCGTGCTCGG CGCGTTCTCG GAAGTGGTCC GCGCGCTGGT TCGGCCGTCC GCGCTTCGGC GCGAGGAAGC AGCCTTGCGT GCCGACGCCG AAGCGATCAT CGCGCGATTC GGCACGCGTC TCACGCCGCG CATCGAGCAA CCGGCGCATA GCCTCTCGTA TGCGAACCGC CGGCGCGTCG AGATTGCGCG TGCGCTGGCG CTGCGGCCGC GCATGTTGCT GCTCGACGAA CCCACCGCGG GAATGAACGA ATCGGAGACC GCGGAAATGC TCGAGCTGAT TCTCGACCTC AAGCGCGATG GACTGACGAT TCTGCTGATC GAACATAAAC TCGATCTGGT GATGCGCCTG TCCGACCGCG TGATCGTTCT CGACGACGGC AGGAAGATCG CAGCGGGTTT GCCTGACGCC GTGCGCAACG ACCCGGCGGT CATCGACGCC TATCTCGGCC ATCGTCATGT GGGCGCGCAA GCCGGACCGG CCGCACAAGC GGTCGCGGCG CGCAGCGACT CCACGGTGAC AGGCGCGCAA CACGCA
Details for BuxeA.00057.s.A1.GE33006
HARVESTED ON: 8/16/2011
SEQUENCED ON: 8/22/2011
EXPECTED MW: 34kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNPASVSGR DILFDVRRVT RRFDGLTAVD GVSLSVARGE CVSVIGPNGA GKSTLFNLLT GMDVPDEGEI RLDGEDVTGT SPERLAARGV ARTFQHGRVF GNLSVLDNVL IGAHSRLALA RPGWPVLGAF SEVVRALVRP SALRREEAAL RADAEAIIAR FGTRLTPRIE QPAHSLSYAN RRRVEIARAL ALRPRMLLLD EPTAGMNESE TAEMLELILD LKRDGLTILL IEHKLDLVMR LSDRVIVLDD XRKIAAGLXD AVRNDPAVID AYLGHRHVGA QAGPAAQAVA ARSDSTVTXA QHA
Validated NT Sequence
attttnttnn ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaacc ctgcatcggt aagcggccgg gacatcctgt tcgacgtgcg gcgcgtgacg cgccgcttcg acggcctgac cgcggtggat ggcgtgagcc tgtcagtggc gcgcggcgaa tgcgtgagcg tgatcggccc gaacggcgcg ggcaagtcga cgctgttcaa tctgctgacc ggcatggatg tgccggacga aggcgagatt cgcctcgacg gtgaggacgt gaccggtact tcgccggaac gcctcgccgc gcgcggcgta gcgcgcacgt ttcagcacgg ccgcgtgttc ggcaacctga gcgtgctcga taacgtgttg atcggcgcgc attcccggct tgcgctggcg cgtccaggct ggcccgtgct cggcgcgttc tcggaagtgg tccgcgcgct ggttcggccg tccgcgcttc ggcgcgagga agcagccttg cgtgccgacg ccgaagcgat catcgcgcga ttcggcacgc gtctcacgcc gcgcatcgag caaccggcgc atagcctctc gtatgcgaac cgccggcgcg tcgagattgc gcgtgcgctg gcgctgcggc cgcgcatgtt gctgctcgac gaacccaccg cgggaatgaa cgaatcggag accgcggaaa tgctcgagct gattctcgac ctcaagcgcg atggactgac gattctgctg atcgaacata aactcgatct ggtgatgcgc ctgtccgacc gcgtgatcgt tctcgacgac ngcaggaaga tcgcagcggg tttgnctgac gccgtgcgca acgacccggc ggtcatcgac gcctatctcg gccatcgtca tgtgggcgcg caagccggac cggccgcnca agcggtcgcg gcgcgcagcg actccacggt gacngnngcg caacacgcat aatnaacagc acgaanagtt ctgcagccaa gctnnncnag gntccggctg ntannnagcc cgaaagganc tnant
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNPASVSGR DILFDVRRVT RRFDGLTAVD GVSLSVARGE CVSVIGPNGA GKSTLFNLLT GMDVPDEGEI RLDGEDVTGT SPERLAARGV ARTFQHGRVF GNLSVLDNVL IGAHSRLALA RPGWPVLGAF SEVVRALVRP SALRREEAAL RADAEAIIAR FGTRLTPRIE QPAHSLSYAN RRRVEIARAL ALRPRMLLLD EPTAGMNESE TAEMLELILD LKRDGLTILL IEHKLDLVMR LSDRVIVLDD GRKIAAGLPD AVRNDPAVID AYLGHRHVGA QAGPAAQAVA ARSDSTVTGA QHA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaccctgca tcggtaagcg gccgggacat cctgttcgac gtgcggcgcg tgacgcgccg cttcgacggc ctgaccgcgg tggatggcgt gagcctgtca gtggcgcgcg gcgaatgcgt gagcgtgatc ggcccgaacg gcgcgggcaa gtcgacgctg ttcaatctgc tgaccggcat ggatgtgccg gacgaaggcg agattcgcct cgacggtgag gacgtgaccg gtacttcgcc ggaacgcctc gccgcgcgcg gcgtagcgcg cacgtttcag cacggccgcg tgttcggcaa cctgagcgtg ctcgataacg tgttgatcgg cgcgcattcc cggcttgcgc tggcgcgtcc aggctggccc gtgctcggcg cgttctcgga agtggtccgc gcgctggttc ggccgtccgc gcttcggcgc gaggaagcag ccttgcgtgc cgacgccgaa gcgatcatcg cgcgattcgg cacgcgtctc acgccgcgca tcgagcaacc ggcgcatagc ctctcgtatg cgaaccgccg gcgcgtcgag attgcgcgtg cgctggcgct gcggccgcgc atgttgctgc tcgacgaacc caccgcggga atgaacgaat cggagaccgc ggaaatgctc gagctgattc tcgacctcaa gcgcgatgga ctgacgattc tgctgatcga acataaactc gatctggtga tgcgcctgtc cgaccgcgtg atcgttctcg acgacggcag gaagatcgca gcgggtttgc ctgacgccgt gcgcaacgac ccggcggtca tcgacgccta tctcggccat cgtcatgtgg gcgcgcaagc cggaccggcc gcacaagcgg tcgcggcgcg cagcgactcc acggtgacag gcgcgcaaca cgcataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa