SSGCID: Target Page

BuxeA.00057.q

ABC branched chain amino acid family transporter, ATPase subunit

CENTER ID:	BuxeA.00057.q
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00057.q.A1.GE33004	Full length( BuxeA.00057.q )		1	262

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.8003
RefSeq:	YP_555496.1
UniProt:	Q13IE3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEQVRTDCIL QVDRLCKRFG GVAAVRDVSF DLKRGEILGL VGPNGAGKTS CFNLITGFYR PDSGDVLFEG RPLTGMKPHR IARAGVVRSF QKTNVLKPLS VFENVLAGHY LAARQSLWRV FFPGQAVRDA ETRVRREAKR IVDLMGLGKR IDSPAYLLSC GELRLLEVAM ALAAKPRLLM LDEPAAGLNT QEAVVLGKVL RELRDREVEA IILVEHNMNL VTNTCDRLVV MNFGEKLAEG APRDILHDPR VVEAYLGKAH AA

NT Sequence

ATGGAACAGG TTCGCACGGA TTGCATCTTG CAGGTGGACC GGTTGTGCAA GCGCTTTGGC GGCGTCGCCG CGGTTCGCGA CGTCAGTTTC GATCTGAAGC GGGGCGAAAT TCTCGGGCTT GTCGGTCCGA ACGGCGCGGG CAAGACCAGT TGCTTTAACC TTATCACCGG GTTTTATCGT CCGGACAGTG GCGACGTGCT GTTCGAAGGA CGTCCGCTGA CGGGCATGAA GCCGCACCGG ATTGCGCGCG CCGGCGTGGT GCGCAGCTTC CAGAAAACCA ACGTGCTCAA GCCGCTGTCG GTGTTCGAGA ACGTTTTGGC TGGTCACTAT CTGGCTGCGC GTCAATCGCT ATGGCGGGTG TTTTTTCCAG GCCAGGCGGT GCGCGATGCG GAAACTCGCG TGCGGCGGGA AGCGAAACGG ATCGTCGATC TGATGGGATT GGGCAAACGC ATCGATTCGC CCGCGTATCT GCTGTCGTGC GGAGAGTTGC GCTTGCTGGA AGTCGCGATG GCGCTGGCCG CCAAGCCGCG CCTGCTGATG CTCGACGAGC CCGCCGCTGG GCTGAACACG CAGGAGGCCG TCGTGCTCGG CAAGGTGCTT CGCGAACTGC GTGACCGGGA AGTCGAGGCG ATCATTCTCG TTGAACACAA CATGAACCTC GTGACGAACA CCTGCGACCG CCTCGTCGTG ATGAACTTCG GCGAAAAGCT CGCCGAAGGC GCGCCACGCG ATATTCTCCA CGATCCGCGG GTGGTCGAAG CTTATCTTGG CAAGGCACAC GCAGCA

Details for BuxeA.00057.q.A1.GE33004

HARVESTED ON:	8/16/2011
SEQUENCED ON:	8/22/2011
EXPECTED MW:	31kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMEQVRTDCI LQVDRLCKRF GGVAAVRDVS FDLKRGEILG LVGPNGAGKT SCFNLITGFY RPDSGDVLFE GRPLTGMKPH RIARAGVVRS FQKTNVLKPL SVFENVLAGH YLAARQSLWR VFFPGQAVRD AETRVRREAK RIVDLMGLGK RIDSPAYLLS CGELRLLEVA MALAAKPRLL MLDEPAAGLN TQEAVVLGKV LRELRDREVE AIILVEHNMN LVTNTCDRLV VMNFGEKLAE XAPRDILHDP RVVEAYLGKA HAA

Validated NT Sequence

ttttntttna ctttaagang gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaac aggttcgcac ggattgcatc ttgcaggtgg accggttgtg caagcgcttt ggcggcgtcg ccgcggttcg cgacgtcagt ttcgatctga agcggggcga aattctcggg cttgtcggtc cgaacggcgc gggcaagacc agttgcttta accttatcac cgggttttat cgtccggaca gtggcgacgt gctgttcgaa ggacgtccgc tgacgggcat gaagccgcac cggattgcgc gcgccggcgt ggtgcgcagc ttccagaaaa ccaacgtgct caagccgctg tcggtgttcg agaacgtttt ggctggtcac tatctggctg cgcgtcaatc gctatggcgg gtgttttttc caggccaggc ggtgcgcgat gcggaaactc gcgtgcggcg ggaagcgaaa cggatcgtcg atctgatggg attgggcaaa cgcatcgatt cgcccgcgta tctgctgtcg tgcggagagt tgcgcttgct ggaagtcgcg atggcgctgg ccgccaagcc gcgcctgctg atgctcgacg agcccgccgc tgggctgaac acgcaggagg ccgtcgtgct cggcaaggtg cttcgcgaac tgcgtgaccg ggaagtcgag gcgatcattc tcgttgaaca caacatgaac ctcgtgacga acacctgcga ccgcctcgtc gtgatgaact tcggcgaaaa gctcgccgaa ngcgcgccac gcgatattct ccacgatccg cgggtggtcg aagcttatct tggcaaggca cacgcagcat aataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctnanttg gctgctgcnn nnnnnnannn na

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaacaggtt cgcacggatt gcatcttgca ggtggaccgg ttgtgcaagc gctttggcgg cgtcgccgcg gttcgcgacg tcagtttcga tctgaagcgg ggcgaaattc tcgggcttgt cggtccgaac ggcgcgggca agaccagttg ctttaacctt atcaccgggt tttatcgtcc ggacagtggc gacgtgctgt tcgaaggacg tccgctgacg ggcatgaagc cgcaccggat tgcgcgcgcc ggcgtggtgc gcagcttcca gaaaaccaac gtgctcaagc cgctgtcggt gttcgagaac gttttggctg gtcactatct ggctgcgcgt caatcgctat ggcgggtgtt ttttccaggc caggcggtgc gcgatgcgga aactcgcgtg cggcgggaag cgaaacggat cgtcgatctg atgggattgg gcaaacgcat cgattcgccc gcgtatctgc tgtcgtgcgg agagttgcgc ttgctggaag tcgcgatggc gctggccgcc aagccgcgcc tgctgatgct cgacgagccc gccgctgggc tgaacacgca ggaggccgtc gtgctcggca aggtgcttcg cgaactgcgt gaccgggaag tcgaggcgat cattctcgtt gaacacaaca tgaacctcgt gacgaacacc tgcgaccgcc tcgtcgtgat gaacttcggc gaaaagctcg ccgaaggcgc gccacgcgat attctccacg atccgcgggt ggtcgaagct tatcttggca aggcacacgc agcataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa