SSGCID: Target Page

BuxeA.00057.m

ABC branched chain amino acid family transporter, ATPase subunit

CENTER ID:	BuxeA.00057.m
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00057.m.A1.GE33000	Full length( BuxeA.00057.m )		1	247

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.8198
RefSeq:	YP_555688.1
UniProt:	Q13HV1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLRIENLCAG YGDVDVVHNV TLDVLPNEVV GVLGCNGAGK TTLVKAVMGL LPRVSGQVSF LDQPVTGLRT HEIAQRGIGL VPQGRWIFPK LTVRENLLMG TQAAGGADIL DEVFDYFPVL KTRLSQQGGT LSGGEQQVLA ISRALCGRPK LLLLDEPSDG VQPNLVERIG EVIPELCRRS KLAVLLVEQN LDLVLQSARR CIVLSNGRLV HAGAVEPRHS GKGEHPLARY LSPAADAAPD FRPENIQ

NT Sequence

ATGCTGCGCA TTGAAAACCT GTGCGCCGGA TATGGCGATG TGGATGTGGT TCACAACGTC ACGCTGGACG TGCTGCCAAA CGAGGTGGTC GGTGTGCTCG GCTGCAACGG CGCGGGCAAG ACCACGCTGG TGAAGGCGGT CATGGGGCTG CTGCCGCGTG TGAGCGGGCA GGTGTCTTTC CTGGACCAGC CGGTGACAGG CCTGCGCACG CACGAGATCG CGCAGCGCGG CATCGGTCTG GTGCCGCAGG GGCGGTGGAT CTTTCCGAAG TTGACCGTGC GGGAGAACCT GCTGATGGGT ACGCAGGCCG CCGGCGGGGC GGACATCCTC GATGAGGTAT TCGATTACTT CCCGGTGCTC AAGACGCGCC TATCGCAGCA GGGCGGCACG CTCTCGGGTG GCGAGCAGCA GGTCCTCGCC ATCTCACGCG CCCTGTGCGG CCGTCCAAAA CTGCTCTTGC TGGATGAGCC CTCCGATGGT GTGCAGCCCA ATCTGGTCGA GCGTATCGGT GAGGTGATTC CCGAGCTCTG CCGCCGCTCG AAACTGGCGG TGCTCCTCGT CGAACAGAAC CTCGACCTCG TCCTTCAGAG CGCACGCCGC TGCATCGTGC TCAGCAACGG GCGACTTGTG CATGCCGGCG CCGTTGAGCC GCGACACAGC GGGAAGGGCG AACACCCGCT CGCGCGCTAT CTCTCCCCGG CCGCGGATGC CGCGCCGGAC TTCCGCCCTG AAAACATCCA G

Details for BuxeA.00057.m.A1.GE33000

HARVESTED ON:	8/16/2011
SEQUENCED ON:	8/22/2011
EXPECTED MW:	28kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLRIENLCA GYGDVDVVHN VTLDVLPNEV VGVLGCNGAG KTTLVKAVMG LLPRVSGQVS FLDQPVTGLR THEIAQRGIG LVPQGRWIFP KLTVRENLLM GTQAAGGADI LDEVFDYFPV LKTRLSQQGG TLSGGEQQVL AISRALCGRP KLLLLDEPSD GVQPNLVERI GEVIPELCRR SKLAVLLVEQ NLDLVLQSAR RCIVLSNGRL VHAGAVEPRH SGKGEHPLAR YLSPAADAAP XFRPENIQ

Validated NT Sequence

attttgttta actttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgctg cgcattgaaa acctgtgcgc cggatatggc gatgtggatg tggttcacaa cgtcacgctg gacgtgctgc caaacgaggt ggtcggtgtg ctcggctgca acggcgcggg caagaccacg ctggtgaagg cggtcatggg gctgctgccg cgtgtgagcg ggcaggtgtc tttcctggac cagccggtga caggcctgcg cacgcacgag atcgcgcagc gcggcatcgg tctggtgccg caggggcggt ggatctttcc gaagttgacc gtgcgggaga acctgctgat gggtacgcag gccgccggcg gggcggacat cctcgatgag gtattcgatt acttcccggt gctcaagacg cgcctatcgc agcagggcgg cacgctctcg ggtggcgagc agcaggtcct cgccatctca cgcgccctgt gcggccgtcc aaaactgctc ttgctggatg agccctccga tggtgtgcag cccaatctgg tcgagcgtat cggtgaggtg attcccgagc tctgccgccg ctcgaaactg gcggtgctcc tcgtcgaaca gaacctcgac ctcgtccttc agagcgcacg ccgctgcatc gtgctcagca acgggcgact tgtgcatgcc ggcgccgttg agccgcgaca cagcgggaag ggcgaacacc cgctcgcgcg ctatctctcc ccggccgcgg atgccgcgcc gganttccgc cctgaaaaca tccagtaata aacagcacga acaagttctg cagccaagct tctcgaggan ccggctgcta acaaagcccg aaaggaagct ganttggctg ctgc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgcgcatt gaaaacctgt gcgccggata tggcgatgtg gatgtggttc acaacgtcac gctggacgtg ctgccaaacg aggtggtcgg tgtgctcggc tgcaacggcg cgggcaagac cacgctggtg aaggcggtca tggggctgct gccgcgtgtg agcgggcagg tgtctttcct ggaccagccg gtgacaggcc tgcgcacgca cgagatcgcg cagcgcggca tcggtctggt gccgcagggg cggtggatct ttccgaagtt gaccgtgcgg gagaacctgc tgatgggtac gcaggccgcc ggcggggcgg acatcctcga tgaggtattc gattacttcc cggtgctcaa gacgcgccta tcgcagcagg gcggcacgct ctcgggtggc gagcagcagg tcctcgccat ctcacgcgcc ctgtgcggcc gtccaaaact gctcttgctg gatgagccct ccgatggtgt gcagcccaat ctggtcgagc gtatcggtga ggtgattccc gagctctgcc gccgctcgaa actggcggtg ctcctcgtcg aacagaacct cgacctcgtc cttcagagcg cacgccgctg catcgtgctc agcaacgggc gacttgtgca tgccggcgcc gttgagccgc gacacagcgg gaagggcgaa cacccgctcg cgcgctatct ctccccggcc gcggatgccg cgccggactt ccgccctgaa aacatccagt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa