SSGCID: Target Page

BuxeA.00057.i

ABC branched chain amino acid family transporter, ATPase subunit

CENTER ID:	BuxeA.00057.i
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00057.i.A1.GE32996	Full length( BuxeA.00057.i )		1	234

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.8325
RefSeq:	YP_555814.1
UniProt:	Q13HH5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLDLGNVNVA YGRRPVIHNM HLKVGEGEAV SVLGPNGAGK TTLLRAISGM IPVKTGSIRL NSQELANASP NAVARHGVMH VAEGRRLFGN MTCRENLMLG GYMRSGPAVN QDFRRLLDVF PEIEPKLDVR AGALSGGQQQ MLAIARGLMS RPKILMLDEP TIGLSPLVVQ RLKTVLTSSA REFFGSLLLV EQDLSLALAV TSRVYVIRNG RIVHEGAVQN IQRENLVEQY FGVA

NT Sequence

ATGCTTGATC TTGGAAATGT GAATGTGGCT TATGGACGGC GACCTGTTAT TCATAATATG CATTTGAAGG TCGGCGAAGG AGAAGCTGTC TCGGTGCTCG GACCGAATGG GGCGGGCAAG ACGACGCTGC TGCGCGCAAT ATCCGGCATG ATACCGGTCA AGACAGGTAG CATTCGCCTG AACAGTCAGG AACTCGCAAA TGCCTCGCCG AACGCCGTTG CGAGGCATGG GGTAATGCAT GTTGCTGAGG GGAGGCGCCT TTTTGGCAAC ATGACATGTC GGGAAAATCT TATGCTGGGG GGCTACATGC GTAGTGGGCC CGCCGTGAAT CAGGATTTCA GGAGGCTGTT GGACGTTTTC CCCGAAATTG AACCGAAGCT CGATGTTCGG GCAGGTGCGC TTTCAGGAGG CCAGCAGCAG ATGCTCGCAA TTGCACGCGG ATTGATGAGC AGACCAAAGA TCCTGATGCT TGACGAGCCG ACGATCGGAC TGAGTCCGCT TGTCGTGCAA AGACTTAAAA CGGTTTTAAC CAGCTCCGCT CGTGAGTTTT TCGGATCTCT ACTCCTGGTT GAGCAGGATC TCTCCCTCGC GCTCGCTGTA ACATCACGCG TCTACGTAAT CCGGAATGGC AGGATCGTCC ATGAGGGCGC TGTCCAGAAT ATCCAGCGAG AGAATCTGGT CGAGCAGTAT TTCGGTGTCG CT

Details for BuxeA.00057.i.A1.GE32996

HARVESTED ON:	8/16/2011
SEQUENCED ON:	8/23/2011
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLDLGNVNV AYGRRPVIHN MHLKVGEGEA VSVLGPNGAG KTTLLRAISG MIPVKTGSIR LNSQELANAS PNAVARHGVM HVAEGRRLFG NMTCRENLML GGYMRSGPAV NQDFRRLLDV FPEIEPKLDV RAGALSGGQQ QMLAIARGLM SRPKILMLDE PTIGLSPLVV QRLKTVLTSS AREFFGSLLL VEQDLSLALA VTSRVYVIRN GRIVHEGAVQ NIQRENLVEQ YFGVA

Validated NT Sequence

ttttnttnnn nttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgcttga tcttggaaat gtgaatgtgg cttatggacg gcgacctgtt attcataata tgcatttgaa ggtcggcgaa ggagaagctg tctcggtgct cggaccgaat ggggcgggca agacgacgct gctgcgcgca atatccggca tgataccggt caagacaggt agcattcgcc tgaacagtca ggaactcgca aatgcctcgc cgaacgccgt tgcgaggcat ggggtaatgc atgttgctga ggggaggcgc ctttttggca acatgacatg tcgggaaaat cttatgctgg ggggctacat gcgtagtggg cccgccgtga atcaggattt caggaggctg ttggacgttt tccccgaaat tgaaccgaag ctcgatgttc gggcaggtgc gctttcagga ggccagcagc agatgctcgc aattgcacgc ggattgatga gcagaccaaa gatcctgatg cttgacgagc cgacgatcgg actgagtccg cttgtcgtgc aaagacttaa aacggtttta accagctccg ctcgtgagtt tttcggatct ctactcctgg ttgagcagga tctctccctc gcgctcgctg taacatcacg cgtctacgta atccggaatg gcaggatcgt ccatgagggc gctgtccaga atatccagcg agagaatctg gtcgagcagt atttcggtgt cgcttaataa acagcacgaa caagttctgc agccnagctt ctcgaggatc cngctgctaa caaagcccga aaggaagctg agttggctgc tgcnnccgnt nannnnnnnn nnnnnnnnaa aaaaagna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcttgatctt ggaaatgtga atgtggctta tggacggcga cctgttattc ataatatgca tttgaaggtc ggcgaaggag aagctgtctc ggtgctcgga ccgaatgggg cgggcaagac gacgctgctg cgcgcaatat ccggcatgat accggtcaag acaggtagca ttcgcctgaa cagtcaggaa ctcgcaaatg cctcgccgaa cgccgttgcg aggcatgggg taatgcatgt tgctgagggg aggcgccttt ttggcaacat gacatgtcgg gaaaatctta tgctgggggg ctacatgcgt agtgggcccg ccgtgaatca ggatttcagg aggctgttgg acgttttccc cgaaattgaa ccgaagctcg atgttcgggc aggtgcgctt tcaggaggcc agcagcagat gctcgcaatt gcacgcggat tgatgagcag accaaagatc ctgatgcttg acgagccgac gatcggactg agtccgcttg tcgtgcaaag acttaaaacg gttttaacca gctccgctcg tgagtttttc ggatctctac tcctggttga gcaggatctc tccctcgcgc tcgctgtaac atcacgcgtc tacgtaatcc ggaatggcag gatcgtccat gagggcgctg tccagaatat ccagcgagag aatctggtcg agcagtattt cggtgtcgct taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa