SSGCID: Target Page

BuxeA.00057.h

ABC branched chain amino acid family transporter, ATPase subunit

CENTER ID:	BuxeA.00057.h
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00057.h.A1.GE32995	Full length( BuxeA.00057.h )		1	241

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.8629
RefSeq:	YP_556093.1
UniProt:	Q13GP6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MALLEVNHLT KRFGGLTAVN DVSFSVDEHQ ILGVIGPNGA GKSTLFTLLT GFTRPSAGEW SIAGVSLHGR NPEQICKAGI VRTFQIVQPF WSMSVFENAM VAAVHRSRSR AQARELAEES LAWVGLSAKR DYPIGSLTIA DKKALEMAKA CASGARVILL DEVMAGLRPG EVDRVVQTVL TLRRERGLTF LVVEHHMDAV MTLSDQILVL NFGALLAKGT PEQIQRDTRV QEAYLGVTLH A

NT Sequence

GTGGCGCTGC TTGAAGTCAA CCATCTCACC AAACGTTTCG GCGGCCTGAC CGCGGTCAAC GACGTATCGT TCTCGGTCGA CGAGCATCAG ATTCTCGGCG TCATCGGTCC CAATGGCGCC GGCAAGAGCA CCCTATTCAC GCTGCTCACC GGCTTCACGC GCCCAAGCGC GGGCGAGTGG TCGATCGCCG GCGTATCGCT GCACGGACGC AATCCGGAGC AGATCTGCAA AGCCGGCATC GTGAGAACGT TCCAGATCGT TCAGCCGTTC TGGAGCATGA GTGTGTTCGA GAACGCGATG GTCGCGGCCG TACATCGGAG CCGGTCACGA GCCCAAGCTC GCGAACTGGC GGAAGAATCG CTCGCCTGGG TCGGACTGTC GGCGAAACGC GACTATCCCA TCGGCAGTCT GACGATCGCC GACAAGAAAG CGCTTGAAAT GGCCAAGGCC TGCGCGTCGG GCGCCCGTGT CATCCTGCTC GACGAAGTCA TGGCCGGCTT GCGGCCTGGC GAAGTCGACC GCGTCGTGCA GACCGTGCTC ACGCTGCGGC GCGAACGCGG CCTCACCTTT CTCGTCGTCG AACATCATAT GGATGCCGTC ATGACCCTGT CCGATCAGAT CCTCGTACTC AATTTCGGCG CGTTGCTCGC CAAAGGAACG CCGGAACAGA TCCAGCGCGA CACGCGGGTC CAGGAAGCGT ATCTGGGAGT GACGCTGCAT GCT

Details for BuxeA.00057.h.A1.GE32995

HARVESTED ON:	8/16/2011
SEQUENCED ON:	8/22/2011
EXPECTED MW:	28kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVALLEVNH LTKRFGGLTA VNDVSFSVDE HQILGVIGPN GAGKSTLFTL LTGFTRPSAG EWSIAGVSLH GRNPEQICKA GIVRTFQIVQ PFWSMSVFEN AMVAAVHRSR SRAQARELAE ESLAWVGLSA KRDYPIGSLT IADKKALEMA KACASGARVI LLDEVMAGLR PGEVDRVVQT VLTLRRERGL TFLVVEHHMD AVMTLSDQIL VLNFGALLAK GTPEQIQRDT RVQEAYLGVT LHA

Validated NT Sequence

attttnnnnn ntttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg cgctgcttga agtcaaccat ctcaccaaac gtttcggcgg cctgaccgcg gtcaacgacg tatcgttctc ggtcgacgag catcagattc tcggcgtcat cggtcccaat ggcgccggca agagcaccct attcacgctg ctcaccggct tcacgcgccc aagcgcgggc gagtggtcga tcgccggcgt atcgctgcac ggacgcaatc cggagcagat ctgcaaagcc ggcatcgtga gaacgttcca gatcgttcag ccgttctgga gcatgagtgt gttcgagaac gcgatggtcg cggccgtaca tcggagccgg tcacgagccc aagctcgcga actggcggaa gaatcgctcg cctgggtcgg actgtcggcg aaacgcgact atcccatcgg cagtctgacg atcgccgaca agaaagcgct tgaaatggcc aaggcctgcg cgtcgggcgc ccgtgtcatc ctgctcgacg aagtcatggc cggcttgcgg cctggcgaag tcgaccgcgt cgtgcagacc gtgctcacgc tgcggcgcga acgcggcctc acctttctcg tcgtcgaaca tcatatggat gccgtcatga ccctgtccga tcagatcctc gtactcaatt tcggcgcgtt gctcgccaaa ggaacgccgg aacagatcca gcgcgacacg cgggtccagg aagcgtatct gggagtgacg ctgcatgctt aataaacagc acgaacaagt tctgcagcca agcttctcga ggatccngct gctaacaaag cccgaaaggn nnctnanttg gntgntgcnn nnnannnnnn nnnaaaaaaa aaaaannnnn nttnaacnnn nnnaangnnc cnnnnnnnnn nnncccncng nnngnnntcn nnnnnnncnt cnnntnnnnn nnnc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggcgctg cttgaagtca accatctcac caaacgtttc ggcggcctga ccgcggtcaa cgacgtatcg ttctcggtcg acgagcatca gattctcggc gtcatcggtc ccaatggcgc cggcaagagc accctattca cgctgctcac cggcttcacg cgcccaagcg cgggcgagtg gtcgatcgcc ggcgtatcgc tgcacggacg caatccggag cagatctgca aagccggcat cgtgagaacg ttccagatcg ttcagccgtt ctggagcatg agtgtgttcg agaacgcgat ggtcgcggcc gtacatcgga gccggtcacg agcccaagct cgcgaactgg cggaagaatc gctcgcctgg gtcggactgt cggcgaaacg cgactatccc atcggcagtc tgacgatcgc cgacaagaaa gcgcttgaaa tggccaaggc ctgcgcgtcg ggcgcccgtg tcatcctgct cgacgaagtc atggccggct tgcggcctgg cgaagtcgac cgcgtcgtgc agaccgtgct cacgctgcgg cgcgaacgcg gcctcacctt tctcgtcgtc gaacatcata tggatgccgt catgaccctg tccgatcaga tcctcgtact caatttcggc gcgttgctcg ccaaaggaac gccggaacag atccagcgcg acacgcgggt ccaggaagcg tatctgggag tgacgctgca tgcttaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa