BuxeA.00057.g

ABC branched chain amino acid family transporter, ATPase subunit

CENTER ID: BuxeA.00057.g
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00057.g.A1.GE32994 Full length( BuxeA.00057.g ) 1 240
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.8630
RefSeq: YP_556094.1
UniProt: Q13GP5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLELIDVHAG YGDTEILHGV SLEVRAGTLV ALVGGNAAGK STLAGSVAGT VPVRGGRVLF EGRDLASLRA HERARAGIVL VPEGRKLFPQ LSVEETLLTS SSFAPARTHR KQNIERMYAL FPRLVERRKQ LAATMSGGEQ QMLAIARGLM SEPRLLILDE PSLGLAPMMV EQIFVAAQAL QAQGLTILLI EQHLNEALKR CDFAYVVENG CVVLSGSGSE LLADARLREA YLGKSGRVAA
NT Sequence
ATGCTTGAAC TCATCGACGT GCATGCCGGC TACGGCGACA CCGAAATCCT GCACGGCGTC TCGCTCGAAG TGCGGGCCGG TACGCTGGTT GCGCTGGTGG GCGGCAATGC CGCCGGCAAA TCGACACTTG CGGGCAGCGT CGCCGGAACC GTTCCGGTGC GCGGCGGGCG AGTTCTGTTC GAAGGTCGCG ACCTTGCGTC GTTACGCGCC CACGAACGCG CGCGGGCCGG TATCGTGCTC GTGCCGGAAG GTCGCAAGCT GTTTCCCCAG CTCTCGGTCG AGGAAACCCT GTTGACCTCC TCCTCGTTCG CACCGGCGCG TACGCATCGC AAGCAGAACA TCGAGCGGAT GTATGCGCTC TTTCCGCGTC TGGTCGAGCG GCGCAAGCAA CTCGCCGCCA CCATGTCCGG CGGCGAACAG CAGATGCTGG CGATCGCCCG TGGTCTGATG TCGGAACCAC GATTGCTGAT TCTGGATGAA CCGTCACTGG GTCTTGCGCC CATGATGGTG GAGCAGATCT TCGTCGCGGC GCAGGCCTTG CAGGCGCAGG GGCTGACCAT CCTGCTGATC GAACAGCATC TGAACGAAGC CTTGAAGCGC TGCGACTTCG CTTACGTCGT GGAAAACGGC TGTGTGGTGC TGTCGGGCTC GGGATCCGAA CTGCTTGCCG ATGCCCGACT GCGCGAGGCC TACCTGGGCA AGTCCGGTCG CGTCGCGGCG
Details for BuxeA.00057.g.A1.GE32994
HARVESTED ON: 8/16/2011
SEQUENCED ON: 8/22/2011
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLELIDVHA GYGDTEILHG VSLEVRAGTL VALVGGNAAG KSTLAGSVAG TVPVRGGRVL FEGRDLASLR AHERARAGIV LVPEGRKLFP QLSVEETLLT SSSFAPARTH RKQNIERMYA LFPRLVERRK QLAATMSGGE QQMLAIARGL MSEPRLLILD EPSLGLAPMM VEQIFVAAXA LQAQGLTILL IEQHLNEALK RCDFAYVVEN GCVVLSGSGS ELLADARLRE AYLGKSGRVA A
Validated NT Sequence
cnnnnnnnnn nnnnntttnn ttnnctttaa gaaggagana taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg cttgaactca tcgacgtgca tgccggctac ggcgacaccg aaatcctgca cggcgtctcg ctcgaagtgc gggccggtac gctggttgcg ctggtgggcg gcaatgccgc cggcaaatcg acacttgcgg gcagcgtcgc cggaaccgtt ccggtgcgcg gcgggcgagt tctgttcgaa ggtcgcgacc ttgcgtcgtt acgcgcccac gaacgcgcgc gggccggtat cgtgctcgtg ccggaaggtc gcaagctgtt tccccagctc tcggtcgagg aaaccctgtt gacctcctcc tcgttcgcac cggcgcgtac gcatcgcaag cagaacatcg agcggatgta tgcgctcttt ccgcgtctgg tcgagcggcg caagcaactc gccgccacca tgtccggcgg cgaacagcag atgctggcga tcgcccgtgg tctgatgtcg gaaccacgat tgctgattct ggatgaaccg tcactgggtc ttgcgcccat gatggtggag cagatcttcg tcgcggcgca ngccttgcag gcgcaggggc tgaccatcct gctgatcgaa cagcatctga acgaagcctt gaagcgctgc gacttcgctt acgtcgtgga aaacggctgt gtggtgctgt cgggctcggg atccgaactg cttgccgatg cccgactgcg cgaggcctac ctgggcaagt ccggtcgcgt cgcggcgtaa taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctnanttggn tgntgnnnnn nnnnncncnc ngnnnnnnnc nanaannnng taangancng ancnnnnnnn ncncnncnnn nnnnnnnnnn annntnnnnn nnnnncnatc cgntntctgn tnnnnaggnn angncanccn nnntttnnnc nnnntcgnnn gnnnccccc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLELIDVHA GYGDTEILHG VSLEVRAGTL VALVGGNAAG KSTLAGSVAG TVPVRGGRVL FEGRDLASLR AHERARAGIV LVPEGRKLFP QLSVEETLLT SSSFAPARTH RKQNIERMYA LFPRLVERRK QLAATMSGGE QQMLAIARGL MSEPRLLILD EPSLGLAPMM VEQIFVAAQA LQAQGLTILL IEQHLNEALK RCDFAYVVEN GCVVLSGSGS ELLADARLRE AYLGKSGRVA A
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcttgaactc atcgacgtgc atgccggcta cggcgacacc gaaatcctgc acggcgtctc gctcgaagtg cgggccggta cgctggttgc gctggtgggc ggcaatgccg ccggcaaatc gacacttgcg ggcagcgtcg ccggaaccgt tccggtgcgc ggcgggcgag ttctgttcga aggtcgcgac cttgcgtcgt tacgcgccca cgaacgcgcg cgggccggta tcgtgctcgt gccggaaggt cgcaagctgt ttccccagct ctcggtcgag gaaaccctgt tgacctcctc ctcgttcgca ccggcgcgta cgcatcgcaa gcagaacatc gagcggatgt atgcgctctt tccgcgtctg gtcgagcggc gcaagcaact cgccgccacc atgtccggcg gcgaacagca gatgctggcg atcgcccgtg gtctgatgtc ggaaccacga ttgctgattc tggatgaacc gtcactgggt cttgcgccca tgatggtgga gcagatcttc gtcgcggcgc aggccttgca ggcgcagggg ctgaccatcc tgctgatcga acagcatctg aacgaagcct tgaagcgctg cgacttcgct tacgtcgtgg aaaacggctg tgtggtgctg tcgggctcgg gatccgaact gcttgccgat gcccgactgc gcgaggccta cctgggcaag tccggtcgcg tcgcggcgta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a