BuxeA.00057.d

ABC branched chain amino acid family transporter, ATPase subunit

CENTER ID: BuxeA.00057.d
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00057.d.A1.GE32991 Full length( BuxeA.00057.d ) 1 271
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.8761
RefSeq: YP_556221.1
UniProt: Q13GB8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMKAAVLHEI EPPSSAVPAA VEKPYVLEVN RLWASYGRIE ALRNLSLRVR AGQIVTIVGA NGAGKSTLLN ALMGTMPCKG EMLYEGQSLH GSSIEERVFR GISLVPETRE LFPDMSVYDN LALGAFTSYV RGERNLAQQI GPVFERFPRL RERRNQNAMT LSGGERQMLA LGRALLSRPR LLMLDEPSLG LAPLIVKDIF EMIGGLRSTG VSILLVEQNA RAALKAADYA YVMENGEVKL EGAASELIND ASVIGAYLGR RSASPGSSRP V
NT Sequence
ATGATGAAAG CGGCTGTACT TCATGAGATC GAGCCGCCGT CGTCAGCCGT CCCAGCCGCG GTGGAAAAAC CCTATGTCCT CGAAGTGAAC CGGCTTTGGG CCAGCTATGG GCGAATTGAA GCATTGCGCA ACCTTTCTCT GCGGGTGCGC GCGGGACAGA TCGTCACCAT CGTGGGGGCC AACGGCGCGG GAAAGTCGAC CCTTCTAAAT GCCCTGATGG GCACCATGCC ATGCAAGGGC GAAATGTTGT ATGAAGGGCA GTCGCTGCAC GGAAGCTCGA TTGAAGAGCG CGTGTTTCGA GGTATCTCGC TCGTGCCGGA GACCCGGGAA CTGTTCCCCG ATATGTCCGT GTACGACAAC CTTGCGCTCG GCGCATTCAC ATCCTACGTG CGCGGCGAGC GCAATCTCGC GCAACAGATC GGGCCGGTCT TCGAGCGCTT TCCTCGCCTG CGCGAGCGCC GCAATCAGAA TGCCATGACC CTGTCCGGTG GCGAAAGGCA AATGCTGGCG CTAGGCCGTG CGCTGCTGTC CCGGCCGCGC TTGTTGATGC TCGACGAACC TAGCCTGGGC CTCGCGCCGC TAATCGTAAA AGATATCTTC GAGATGATTG GCGGACTACG CTCGACGGGC GTGTCGATTC TTCTCGTCGA ACAGAATGCG CGCGCGGCGC TCAAGGCCGC CGACTACGCG TATGTGATGG AGAACGGAGA AGTAAAACTG GAAGGCGCAG CGAGCGAACT CATTAATGAC GCCTCTGTCA TTGGCGCCTA TCTTGGACGG CGTAGCGCCT CGCCTGGATC ATCCAGACCG GTA
Details for BuxeA.00057.d.A1.GE32991
HARVESTED ON: 8/16/2011
SEQUENCED ON: 8/22/2011
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMMKAAVLHE IEPPSSAVPA AVEKPYVLEV NRLWASYGRI EALRNLSLRV RAGQIVTIVG ANGAGKSTLL NALMGTMPCK GEMLYEGQSL HGSSIEERVF RGISLVPETR ELFPDMSVYD NLALGAFTSY VRGERNLAQQ IGPVFERFPR LRERRNQNAM TLSGGERQML ALGRALLSRP RLLMLDEPSL GLAPLIVKDI FEMIGGLRST GVSILLVEQN ARAALKAADY AYVMENGEVK LEGAASELIN DASVIGAYLG RRXASPGSSR PV
Validated NT Sequence
tttntttanc tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgatgaa agcggctgta cttcatgaga tcgagccgcc gtcgtcagcc gtcccagccg cggtggaaaa accctatgtc ctcgaagtga accggctttg ggccagctat gggcgaattg aagcattgcg caacctttct ctgcgggtgc gcgcgggaca gatcgtcacc atcgtggggg ccaacggcgc gggaaagtcg acccttctaa atgccctgat gggcaccatg ccatgcaagg gcgaaatgtt gtatgaaggg cagtcgctgc acggaagctc gattgaagag cgcgtgtttc gaggtatctc gctcgtgccg gagacccggg aactgttccc cgatatgtcc gtgtacgaca accttgcgct cggcgcattc acatcctacg tgcgcggcga gcgcaatctc gcgcaacaga tcgggccggt cttcgagcgc tttcctcgcc tgcgcgagcg ccgcaatcag aatgccatga ccctgtccgg tggcgaaagg caaatgctgg cgctaggccg tgcgctgctg tcccggccgc gcttgttgat gctcgacgaa cctagcctgg gcctcgcgcc gctaatcgta aaagatatct tcgagatgat tggcggacta cgctcgacgg gcgtgtcgat tcttctcgtc gaacagaatg cgcgcgcggc gctcaaggcc gccgactacg cgtatgtgat ggagaacgga gaagtaaaac tggaaggcgc agcgagcgaa ctcattaatg acgcctctgt cattggcgcc tatctnggac ggcgtancgc ctcgcctgga tcatccagac cggtataata aacagcacga acaagttctg cagccaagct tctcgaggat nccggctgct aacaaagccc gaaaggaact gagttggctg ctgcnnnnnn nanannnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMMKAAVLHE IEPPSSAVPA AVEKPYVLEV NRLWASYGRI EALRNLSLRV RAGQIVTIVG ANGAGKSTLL NALMGTMPCK GEMLYEGQSL HGSSIEERVF RGISLVPETR ELFPDMSVYD NLALGAFTSY VRGERNLAQQ IGPVFERFPR LRERRNQNAM TLSGGERQML ALGRALLSRP RLLMLDEPSL GLAPLIVKDI FEMIGGLRST GVSILLVEQN ARAALKAADY AYVMENGEVK LEGAASELIN DASVIGAYLG RRSASPGSSR PV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatgaaagcg gctgtacttc atgagatcga gccgccgtcg tcagccgtcc cagccgcggt ggaaaaaccc tatgtcctcg aagtgaaccg gctttgggcc agctatgggc gaattgaagc attgcgcaac ctttctctgc gggtgcgcgc gggacagatc gtcaccatcg tgggggccaa cggcgcggga aagtcgaccc ttctaaatgc cctgatgggc accatgccat gcaagggcga aatgttgtat gaagggcagt cgctgcacgg aagctcgatt gaagagcgcg tgtttcgagg tatctcgctc gtgccggaga cccgggaact gttccccgat atgtccgtgt acgacaacct tgcgctcggc gcattcacat cctacgtgcg cggcgagcgc aatctcgcgc aacagatcgg gccggtcttc gagcgctttc ctcgcctgcg cgagcgccgc aatcagaatg ccatgaccct gtccggtggc gaaaggcaaa tgctggcgct aggccgtgcg ctgctgtccc ggccgcgctt gttgatgctc gacgaaccta gcctgggcct cgcgccgcta atcgtaaaag atatcttcga gatgattggc ggactacgct cgacgggcgt gtcgattctt ctcgtcgaac agaatgcgcg cgcggcgctc aaggccgccg actacgcgta tgtgatggag aacggagaag taaaactgga aggcgcagcg agcgaactca ttaatgacgc ctctgtcatt ggcgcctatc ttggacggcg tagcgcctcg cctggatcat ccagaccggt ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa