SSGCID: Target Page

BuxeA.00057.c

ABC branched chain amino acid family transporter, ATPase subunit

CENTER ID:	BuxeA.00057.c
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00057.c.A1.GE32990	Full length( BuxeA.00057.c )		1	234

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.9003
RefSeq:	YP_556462.1
UniProt:	Q13FM7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MADDGILLDA QGISAGYGLS RVLFDVDLQV PRVGGVTILG RNGAGKTTLL KTLVGELPFQ GGSVELAGLP LSGLRTHERI KSGIGYVPQE GGVFAGLSVQ ENLELGIARQ RRHGDIALAF AWFPKLQQRL KQTAGTLSGG ERKMLAIARA LLGAPRILLL DEPTEGMWFG VIDEIAEQLV QLASRIAVVT VEQNLGLAWK ISDHVHVLDR GRIVLSGPTS QVREDPRLLQ LLAP

NT Sequence

TTGGCTGACG ACGGAATCTT GCTGGACGCG CAAGGTATCA GCGCTGGTTA CGGGCTTAGC CGTGTTTTGT TCGACGTCGA TCTGCAAGTG CCTCGCGTTG GCGGAGTCAC GATTCTTGGG CGTAATGGCG CGGGCAAAAC CACCTTGTTG AAAACACTCG TCGGAGAGTT GCCTTTCCAG GGCGGCTCGG TCGAGTTGGC GGGCCTGCCG TTGTCCGGGC TGCGTACCCA TGAGCGGATC AAAAGCGGTA TCGGCTACGT TCCCCAGGAG GGCGGTGTAT TCGCCGGGCT GAGCGTCCAG GAAAATCTCG AACTGGGTAT CGCCCGACAA CGCAGGCATG GTGACATTGC TCTCGCGTTC GCCTGGTTTC CCAAACTTCA GCAACGCCTG AAGCAGACCG CCGGAACGCT GTCGGGCGGC GAACGAAAAA TGCTTGCCAT TGCGCGCGCG CTGCTTGGCG CGCCGCGCAT CCTTTTGCTG GATGAGCCGA CCGAGGGGAT GTGGTTCGGC GTCATAGATG AGATTGCAGA ACAACTGGTG CAACTAGCGA GCCGCATTGC TGTCGTGACC GTCGAGCAGA ACCTCGGACT GGCATGGAAG ATAAGCGATC ACGTTCATGT GCTGGATCGT GGCCGCATCG TGTTGTCCGG CCCAACTTCC CAGGTTCGCG AGGATCCTCG CCTCCTGCAA TTACTGGCGC CT

Details for BuxeA.00057.c.A1.GE32990

HARVESTED ON:	8/16/2011
SEQUENCED ON:	8/22/2011
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLADDGILL DAQGISAGYG LSRVLFDVDL QVPRVGGVTI LGRNGAGKTT LLKTLVGELP FQGGSVELAG LPLSGLRTHE RIKSGIGYVP QEGGVFAGLS VQENLELGIA RQRRHGDIAL AFAWFPKLQQ RLKQTAGTLS GGERKMLAIA RALLGAPRIL LLDEPTEGMW FGVIDEIAEQ LVQLASRIAV VTVEQNLGLA WKISDHVHVL DRGRIVLSGP TSQVREDPRL LQLLAP

Validated NT Sequence

antttntttn nctttaagan ggaganatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgttg gctgacgacg gaatcttgct ggacgcgcaa ggtatcagcg ctggttacgg gcttagccgt gttttgttcg acgtcgatct gcaagtgcct cgcgttggcg gagtcacgat tcttgggcgt aatggcgcgg gcaaaaccac cttgttgaaa acactcgtcg gagagttgcc tttccagggc ggctcggtcg agttggcggg cctgccgttg tccgggctgc gtacccatga gcggatcaaa agcggtatcg gctacgttcc ccaggagggc ggtgtattcg ccgggctgag cgtccaggaa aatctcgaac tgggtatcgc ccgacaacgc aggcatggtg acattgctct cgcgttcgcc tggtttccca aacttcagca acgcctgaag cagaccgccg gaacgctgtc gggcggcgaa cgaaaaatgc ttgccattgc gcgcgcgctg cttggcgcgc cgcgcatcct tttgctggat gagccgaccg aggggatgtg gttcggcgtc atagatgaga ttgcagaaca actggtgcaa ctagcgagcc gcattgctgt cgtgaccgtc gagcagaacc tcggactggc atggaagata agcgatcacg ttcatgtgct ggatcgtggc cgcatcgtgt tgtccggccc aacttcccag gttcgcgagg atcctcgcct cctgcaatta ctggcgcctt aataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctganttg gctgctgcnn nnnnnnnnnn nannnnnnnn aaannnaann nnttnnnnnn nnannnnnnn nngnncnnnn nnnnnnccgc nnnnnnnnnn nngnnnnnnn nnnaagna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttggctgac gacggaatct tgctggacgc gcaaggtatc agcgctggtt acgggcttag ccgtgttttg ttcgacgtcg atctgcaagt gcctcgcgtt ggcggagtca cgattcttgg gcgtaatggc gcgggcaaaa ccaccttgtt gaaaacactc gtcggagagt tgcctttcca gggcggctcg gtcgagttgg cgggcctgcc gttgtccggg ctgcgtaccc atgagcggat caaaagcggt atcggctacg ttccccagga gggcggtgta ttcgccgggc tgagcgtcca ggaaaatctc gaactgggta tcgcccgaca acgcaggcat ggtgacattg ctctcgcgtt cgcctggttt cccaaacttc agcaacgcct gaagcagacc gccggaacgc tgtcgggcgg cgaacgaaaa atgcttgcca ttgcgcgcgc gctgcttggc gcgccgcgca tccttttgct ggatgagccg accgagggga tgtggttcgg cgtcatagat gagattgcag aacaactggt gcaactagcg agccgcattg ctgtcgtgac cgtcgagcag aacctcggac tggcatggaa gataagcgat cacgttcatg tgctggatcg tggccgcatc gtgttgtccg gcccaacttc ccaggttcgc gaggatcctc gcctcctgca attactggcg ccttaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa