BuxeA.00051.c

Response regulator receiver domain protein(CheY)

CENTER ID: BuxeA.00051.c
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00051.c.B1.GE39492 Full length( BuxeA.00051.c ) 1 150
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.5121
RefSeq: YP_552763.1
UniProt: Q13R76

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAILPAPLSR PGREHPRPGA TDISGISKMS RVLLVDDQPE ALSALRAVLV GRGYTVATAA DGAEAFERLQ RTRVSAVVCD WRMPNMDGAE LIESMQARSE LASVPVILTS GSGEVPAFPV KGFLRKPFAL DKLLSLLAEC ESDAATPAVC
NT Sequence
ATGGCGATTT TGCCTGCACC CCTGTCGCGA CCGGGCCGCG AACACCCGAG GCCCGGTGCA ACCGATATCT CAGGCATAAG CAAAATGAGT CGCGTATTAC TGGTCGACGA CCAACCCGAG GCATTGTCCG CGCTGCGCGC CGTATTGGTC GGCCGTGGTT ACACGGTCGC CACGGCGGCG GATGGCGCCG AAGCTTTTGA ACGACTGCAA CGCACGCGCG TGAGCGCCGT CGTGTGTGAC TGGCGCATGC CCAACATGGA CGGCGCCGAG TTGATCGAAT CGATGCAGGC CCGCAGTGAA CTGGCGTCCG TACCCGTCAT TCTGACGAGC GGCAGCGGCG AGGTGCCGGC CTTTCCCGTC AAAGGCTTTC TCAGGAAACC GTTCGCGCTG GACAAGCTGC TCTCGTTGCT GGCCGAATGC GAAAGCGACG CGGCGACGCC TGCCGTCTGC
Details for BuxeA.00051.c.B1.GE39492
HARVESTED ON: data unavailable
SEQUENCED ON: 9/16/2015
EXPECTED MW: 17kDa
OBSERVED MW: data unavailable
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: None
TOTAL EXPRESSION LEVEL: Data Unavailable
SOLUBLE EXPRESSION LEVEL Data Unavailable
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA ILPAPLSRPG REHPRPGATD ISGISKMSRV LLVDDQPEAL SALRAVLVGR GYTVATAADG AEAFERLQRT RVSAVVCDWR MPNMDGAELI ESMQARSELA SVPVILTSGS GEVPAFPVKG FLRKPFALDK LLSLLAECES DAATPAVC
Validated NT Sequence
atcttactca cttagcagac ggcaggcgtc gccgcgtcgc tttcgcattc ggccagcaac gagagcagct tgtccagcgc gaacggtttc ctgagaaagc ctttgacggg aaaggccggc acctcgccgc tgccgctcgt cagaatgacg ggtacggacg ccagttcact gcgggcctgc atcgattcga tcaactcggc gccgtccatg ttgggcatgc gccagtcaca cacgacggcg ctcacgcgcg tgcgttgcag tcgttcaaaa gcttcggcgc catccgccgc cgtggcgacc gtgtaaccac ggccgaccaa tacggcgcgc agcgcggaca atgcctcggg ttggtcgtcg accagtaata cgcgactcat tttgcttatg cctgagatat cggttgcacc gggcctcggg tgttcgcggc ccggtcgcga caggggtgca ggcaaaatcg ccatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMA ILPAPLSRPG REHPRPGATD ISGISKMSRV LLVDDQPEAL SALRAVLVGR GYTVATAADG AEAFERLQRT RVSAVVCDWR MPNMDGAELI ESMQARSELA SVPVILTSGS GEVPAFPVKG FLRKPFALDK LLSLLAECES DAATPAVC
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcga ttttgcctgc acccctgtcg cgaccgggcc gcgaacaccc gaggcccggt gcaaccgata tctcaggcat aagcaaaatg agtcgcgtat tactggtcga cgaccaaccc gaggcattgt ccgcgctgcg cgccgtattg gtcggccgtg gttacacggt cgccacggcg gcggatggcg ccgaagcttt tgaacgactg caacgcacgc gcgtgagcgc cgtcgtgtgt gactggcgca tgcccaacat ggacggcgcc gagttgatcg aatcgatgca ggcccgcagt gaactggcgt ccgtacccgt cattctgacg agcggcagcg gcgaggtgcc ggcctttccc gtcaaaggct ttctcaggaa accgttcgcg ctggacaagc tgctctcgtt gctggccgaa tgcgaaagcg acgcggcgac gcctgccgtc tgctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat