BuxeA.00046.c

Alpha,alpha-trehalose-phosphate synthase(UDP-forming)

CENTER ID: BuxeA.00046.c
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00046.c.B1.GE39360 Full length( BuxeA.00046.c ) 1 470
BuxeA.00046.c.B11.GE39361 Full length( BuxeA.00046.c ) 1 470
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00046.c.B11.PS02519 Full length( BuxeA.00046.c ) 1 470

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.2279
RefSeq: YP_558765.1
UniProt: Q13YX6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGRLIVVSNR VATPTETKGS AGGLAVGVFG ALKDAGGVWF GWSGDVVSET VANAGPTLEQ DGAVTFATVG LTRKDYDQYY RGFSNATLWP VFHYRNDLAR YEREEYTGYR RVNAWLAHKL IKLLEPDDVI WIHDYHLIPF AEALRNEGVT NRIGFFLHIP FPAPQILLNI PPHEELVKSL SCYDLLGFQT ATDELAFKDY VTRHARGTVS ADGLVEAFGR KLRTGVYRIG VFPDEIAEQA KRYESRQHVL DLKQSLEGRK LIMSVDRLDY SKGLVERFRA FEHLLERSPE WRGNVTLVQI APPTRSDVAT YQKIRQDLEY EAGRINGRYS GLDYTPIRYL NQQYDRWKLM SLFRESQIGF VTPLHDGMNL VAKEYVAAQN PDDPGVLVLS IFAGAAAELS GALLVNPHDA IGMCEALQRA LQMPLDARQR RHEINMAALR KNDLGVWRDT FLHDLRSVPV QQPGKGGGHN
NT Sequence
ATGGGGCGAT TGATCGTCGT GTCGAATCGC GTCGCAACGC CGACGGAAAC CAAGGGATCG GCCGGCGGCC TCGCGGTCGG CGTATTCGGC GCGCTGAAGG ATGCCGGAGG CGTGTGGTTC GGCTGGAGCG GCGACGTGGT CAGCGAGACC GTCGCGAACG CCGGTCCGAC GCTGGAGCAG GACGGCGCGG TGACCTTCGC GACCGTAGGG CTCACGCGCA AGGATTACGA CCAGTACTAT CGCGGCTTTT CGAACGCGAC ATTGTGGCCG GTGTTCCACT ACCGCAACGA CCTCGCGCGC TACGAGCGCG AGGAATACAC GGGCTACCGG CGCGTCAACG CGTGGCTCGC GCACAAGCTG ATCAAGCTGC TCGAACCCGA CGACGTGATC TGGATTCACG ACTACCACCT GATCCCGTTC GCCGAGGCGC TGCGCAACGA GGGTGTCACG AACCGCATCG GCTTCTTCCT GCACATTCCG TTTCCCGCGC CGCAGATCCT GCTCAACATT CCGCCGCACG AGGAACTGGT GAAGTCGCTG TCGTGCTACG ACCTGCTGGG CTTCCAGACC GCCACCGACG AACTCGCGTT CAAGGATTAC GTCACGCGCC ACGCGCGCGG CACGGTGAGT GCGGACGGTC TCGTCGAAGC GTTCGGCCGC AAGCTGCGCA CCGGCGTCTA CCGGATCGGC GTGTTCCCCG ACGAAATCGC CGAGCAGGCG AAGCGCTACG AAAGCCGTCA GCACGTGCTC GACCTGAAGC AGAGCCTGGA AGGGCGCAAG CTGATCATGA GCGTCGACCG GCTGGACTAT TCGAAGGGAC TGGTCGAGCG GTTCCGGGCC TTCGAGCATC TGCTGGAGCG CTCGCCGGAA TGGCGCGGCA ACGTCACGCT GGTGCAGATC GCGCCGCCCA CCCGTTCGGA CGTCGCCACC TATCAGAAGA TTCGCCAGGA TCTGGAGTAC GAAGCGGGGC GCATCAATGG CCGCTACTCG GGTCTCGACT ACACGCCGAT CCGCTATCTG AATCAGCAGT ACGACCGCTG GAAGCTGATG TCGCTGTTTC GCGAATCGCA GATCGGTTTC GTCACGCCGC TGCACGACGG TATGAATCTG GTCGCGAAGG AATACGTCGC CGCGCAGAAT CCCGACGATC CCGGCGTGCT GGTGCTGTCG ATTTTCGCGG GTGCGGCGGC GGAGCTGAGC GGCGCGCTGC TCGTCAATCC ACATGATGCG ATCGGCATGT GCGAAGCCTT GCAGCGCGCG TTGCAGATGC CGCTCGACGC ACGTCAGCGG CGCCATGAAA TCAACATGGC CGCGCTGCGC AAGAACGATC TCGGCGTGTG GCGCGACACG TTCCTGCACG ATCTGCGCAG CGTGCCGGTG CAGCAGCCAG GCAAGGGTGG CGGGCATAAC
Details for BuxeA.00046.c.B1.GE39360
HARVESTED ON: 7/10/2015
SEQUENCED ON: 7/6/2015
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG RLIVVSNRVA TPTETKGSAG GLAVGVFGAL KDAGGVWFGW SGDVVSETVA NAGPTLEQDG AVTFATVGLT RKDYDQYYRG FSNATLWPVF HYRNDLARYE REEYTGYRRV NAWLAHKLIK LLEPDDVIWI HDYHLIPFAE ALRNEGVTNR IGFFLHIPFP APQILLNIPP HEELVKSLSC YDLLGFQTAT DELAFKDYVT RHARGTVSAD GLVEAFGRKL RTGVYRIGVF PDEIAEQAKR YESRQHVLDL KQSLEGRKLI MSVDRLDYSK GLVERFRAFE HLLERSPEWR GNVTLVQIAP PTRSDVATYQ KIRQDLEYEA GRINGRYSGL DYTPIRYLNQ QYDRWKLMSL FRESQIGFVT PLHDGMNLVA KEYVAAQNPD DPGVLVLSIF AGAAAELSGA LLVNPHDAIG MCEALQRALQ MPLDARQRRH EINMAALRKN DLGVWRDTFL HDLRSVPVQQ PGKGGGHN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggggc gattgatcgt cgtgtcgaat cgcgtcgcaa cgccgacgga aaccaaggga tcggccggcg gcctcgcggt cggcgtattc ggcgcgctga aggatgccgg aggcgtgtgg ttcggctgga gcggcgacgt ggtcagcgag accgtcgcga acgccggtcc gacgctggag caggacggcg cggtgacctt cgcgaccgta gggctcacgc gcaaggatta cgaccagtac tatcgcggct tttcgaacgc gacattgtgg ccggtgttcc actaccgcaa cgacctcgcg cgctacgagc gcgaggaata cacgggctac cggcgcgtca acgcgtggct cgcgcacaag ctgatcaagc tgctcgaacc cgacgacgtg atctggattc acgactacca cctgatcccg ttcgccgagg cgctgcgcaa cgagggtgtc acgaaccgca tcggcttctt cctgcacatt ccgtttcccg cgccgcagat cctgctcaac attccgccgc acgaggaact ggtgaagtcg ctgtcgtgct acgacctgct gggcttccag accgccaccg acgaactcgc gttcaaggat tacgtcacgc gccacgcgcg cggcacggtg agtgcggacg gtctcgtcga agcgttcggc cgcaagctgc gcaccggcgt ctaccggatc ggcgtgttcc ccgacgaaat cgccgagcag gcgaagcgct acgaaagccg tcagcacgtg ctcgacctga agcagagcct ggaagggcgc aagctgatca tgagcgtcga ccggctggac tattcgaagg gactggtcga gcggttccgg gccttcgagc atctgctgga gcgctcgccg gaatggcgcg gcaacgtcac gctggtgcag atcgcgccgc ccacccgttc ggacgtcgcc acctatcaga agattcgcca ggatctggag tacgaagcgg ggcgcatcaa tggccgctac tcgggtctcg actacacgcc gatccgctat ctgaatcagc agtacgaccg ctggaagctg atgtcgctgt ttcgcgaatc gcagatcggt ttcgtcacgc cgctgcacga cggtatgaat ctggtcgcga aggaatacgt cgccgcgcag aatcccgacg atcccggcgt gctggtgctg tcgattttcg cgggtgcggc ggcggagctg agcggcgcgc tgctcgtcaa tccacatgat gcgatcggca tgtgcgaagc cttgcagcgc gcgttgcaga tgccgctcga cgcacgtcag cggcgccatg aaatcaacat ggccgcgctg cgcaagaacg atctcggcgt gtggcgcgac acgttcctgc acgatctgcg cagcgtgccg gtgcagcagc caggcaaggg tggcgggcat aactgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for BuxeA.00046.c.B11.GE39361
HARVESTED ON: 7/10/2015
SEQUENCED ON: 7/6/2015
EXPECTED MW: 54kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG RLIVVSNRVA TPTETKGSAG GLAVGVFGAL KDAGGVWFGW SGDVVSETVA NAGPTLEQDG AVTFATVGLT RKDYDQYYRG FSNATLWPVF HYRNDLARYE REEYTGYRRV NAWLAHKLIK LLEPDDVIWI HDYHLIPFAE ALRNEGVTNR IGFFLHIPFP APQILLNIPP HEELVKSLSC YDLLGFQTAT DELAFKDYVT RHARGTVSAD GLVEAFGRKL RTGVYRIGVF PDEIAEQAKR YESRQHVLDL KQSLEGRKLI MSVDRLDYSK GLVERFRAFE HLLERSPEWR GNVTLVQIAP PTRSDVATYQ KIRQDLEYEA GRINGRYSGL DYTPIRYLNQ QYDRWKLMSL FRESQIGFVT PLHDGMNLVA KEYVAAQNPD DPGVLVLSIF AGAAAELSGA LLVNPHDAIG MCEALQRALQ MPLDARQRRH EINMAALRKN DLGVWRDTFL HDLRSVPVQ
Validated NT Sequence
tgctgcaccg gcacgctgcg cagatcgtgc aggaacgtgt cgcgccacac gccgagatcg ttcttgcgca gcgcggccat gttgatttca tggcgccgct gacgtgcgtc gagcggcatc tgcaacgcgc gctgcaaggc ttcgcacatg ccgatcgcat catgtggatt gacgagcagc gcgccgctca gctccgccgc cgcacccgcg aaaatcgaca gcaccagcac gccgggatcg tcgggattct gcgcggcgac gtattccttc gcgaccagat tcataccgtc gtgcagcggc gtgacgaaac cgatctgcga ttcgcgaaac agcgacatca gcttccagcg gtcgtactgc tgattcagat agcggatcgg cgtgtagtcg agacccgagt agcggccatt gatgcgcccc gcttcgtact ccagatcctg gcgaatcttc tgataggtgg cgacgtccga acgggtgggc ggcgcgatct gcaccagcgt gacgttgccg cgccattccg gcgagcgctc cagcagatgc tcgaaggccc ggaaccgctc gaccagtccc ttcgaatagt ccagccggtc gacgctcatg atcagcttgc gcccttccag gctctgcttc aggtcgagca cgtgctgacg gctttcgtag cgcttcgcct gctcggcgat ttcgtcgggg aacacgccga tccggtagac gccggtgcgc agcttgcggc cgaacgcttc gacgagaccg tccgcactca ccgtgccgcg cgcgtggcgc gtgacgtaat ccttgaacgc gagttcgtcg gtggcggtct ggaagcccag caggtcgtag cacgacagcg acttcaccag ttcctcgtgc ggcggaatgt tgagcaggat ctgcggcgcg ggaaacggaa tgtgcaggaa gaagccgatg cggttcgtga caccctcgtt gcgcagcgcc tcggcgaacg ggatcaggtg gtagtcgtga atccagatca cgtcgtcggg ttcgagcagc ttgatcagct tgtgcgcgag ccacgcgttg acgcgccggt agcccgtgta ttcctcgcgc tcgtagcgcg cgaggtcgtt gcggtagtgg aacaccggcc acaatgtcgc gttcgaaaag ccgcgatagt actggtcgta atccttgcgc gtgagcccta cggtcgcgaa ggtcaccgcg ccgtcctgct ccagcgtcgg accggcgttc gcgacggtct cgctgaccac gtcgccgctc cagccgaacc acacgcctcc ggcatccttc agcgcgccga atacgccgac cgcgaggccg ccggccgatc ccttggtttc cgtcggcgtt gcgacgcgat tcgacacgac gatcaatcgc cccatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMG RLIVVSNRVA TPTETKGSAG GLAVGVFGAL KDAGGVWFGW SGDVVSETVA NAGPTLEQDG AVTFATVGLT RKDYDQYYRG FSNATLWPVF HYRNDLARYE REEYTGYRRV NAWLAHKLIK LLEPDDVIWI HDYHLIPFAE ALRNEGVTNR IGFFLHIPFP APQILLNIPP HEELVKSLSC YDLLGFQTAT DELAFKDYVT RHARGTVSAD GLVEAFGRKL RTGVYRIGVF PDEIAEQAKR YESRQHVLDL KQSLEGRKLI MSVDRLDYSK GLVERFRAFE HLLERSPEWR GNVTLVQIAP PTRSDVATYQ KIRQDLEYEA GRINGRYSGL DYTPIRYLNQ QYDRWKLMSL FRESQIGFVT PLHDGMNLVA KEYVAAQNPD DPGVLVLSIF AGAAAELSGA LLVNPHDAIG MCEALQRALQ MPLDARQRRH EINMAALRKN DLGVWRDTFL HDLRSVPVQQ PGKGGGHN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggggc gattgatcgt cgtgtcgaat cgcgtcgcaa cgccgacgga aaccaaggga tcggccggcg gcctcgcggt cggcgtattc ggcgcgctga aggatgccgg aggcgtgtgg ttcggctgga gcggcgacgt ggtcagcgag accgtcgcga acgccggtcc gacgctggag caggacggcg cggtgacctt cgcgaccgta gggctcacgc gcaaggatta cgaccagtac tatcgcggct tttcgaacgc gacattgtgg ccggtgttcc actaccgcaa cgacctcgcg cgctacgagc gcgaggaata cacgggctac cggcgcgtca acgcgtggct cgcgcacaag ctgatcaagc tgctcgaacc cgacgacgtg atctggattc acgactacca cctgatcccg ttcgccgagg cgctgcgcaa cgagggtgtc acgaaccgca tcggcttctt cctgcacatt ccgtttcccg cgccgcagat cctgctcaac attccgccgc acgaggaact ggtgaagtcg ctgtcgtgct acgacctgct gggcttccag accgccaccg acgaactcgc gttcaaggat tacgtcacgc gccacgcgcg cggcacggtg agtgcggacg gtctcgtcga agcgttcggc cgcaagctgc gcaccggcgt ctaccggatc ggcgtgttcc ccgacgaaat cgccgagcag gcgaagcgct acgaaagccg tcagcacgtg ctcgacctga agcagagcct ggaagggcgc aagctgatca tgagcgtcga ccggctggac tattcgaagg gactggtcga gcggttccgg gccttcgagc atctgctgga gcgctcgccg gaatggcgcg gcaacgtcac gctggtgcag atcgcgccgc ccacccgttc ggacgtcgcc acctatcaga agattcgcca ggatctggag tacgaagcgg ggcgcatcaa tggccgctac tcgggtctcg actacacgcc gatccgctat ctgaatcagc agtacgaccg ctggaagctg atgtcgctgt ttcgcgaatc gcagatcggt ttcgtcacgc cgctgcacga cggtatgaat ctggtcgcga aggaatacgt cgccgcgcag aatcccgacg atcccggcgt gctggtgctg tcgattttcg cgggtgcggc ggcggagctg agcggcgcgc tgctcgtcaa tccacatgat gcgatcggca tgtgcgaagc cttgcagcgc gcgttgcaga tgccgctcga cgcacgtcag cggcgccatg aaatcaacat ggccgcgctg cgcaagaacg atctcggcgt gtggcgcgac acgttcctgc acgatctgcg cagcgtgccg gtgcagcagc caggcaaggg tggcgggcat aactgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for BuxeA.00046.c.B11.PS02519
PURIFICATION DATe: 10/20/2015
CONCENTRATION: 32.18mg/ml
OBSERVED MW: 55kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG RLIVVSNRVA TPTETKGSAG GLAVGVFGAL KDAGGVWFGW SGDVVSETVA NAGPTLEQDG AVTFATVGLT RKDYDQYYRG FSNATLWPVF HYRNDLARYE REEYTGYRRV NAWLAHKLIK LLEPDDVIWI HDYHLIPFAE ALRNEGVTNR IGFFLHIPFP APQILLNIPP HEELVKSLSC YDLLGFQTAT DELAFKDYVT RHARGTVSAD GLVEAFGRKL RTGVYRIGVF PDEIAEQAKR YESRQHVLDL KQSLEGRKLI MSVDRLDYSK GLVERFRAFE HLLERSPEWR GNVTLVQIAP PTRSDVATYQ KIRQDLEYEA GRINGRYSGL DYTPIRYLNQ QYDRWKLMSL FRESQIGFVT PLHDGMNLVA KEYVAAQNPD DPGVLVLSIF AGAAAELSGA LLVNPHDAIG MCEALQRALQ MPLDARQRRH EINMAALRKN DLGVWRDTFL HDLRSVPVQ
Validated NT Sequence
tgctgcaccg gcacgctgcg cagatcgtgc aggaacgtgt cgcgccacac gccgagatcg ttcttgcgca gcgcggccat gttgatttca tggcgccgct gacgtgcgtc gagcggcatc tgcaacgcgc gctgcaaggc ttcgcacatg ccgatcgcat catgtggatt gacgagcagc gcgccgctca gctccgccgc cgcacccgcg aaaatcgaca gcaccagcac gccgggatcg tcgggattct gcgcggcgac gtattccttc gcgaccagat tcataccgtc gtgcagcggc gtgacgaaac cgatctgcga ttcgcgaaac agcgacatca gcttccagcg gtcgtactgc tgattcagat agcggatcgg cgtgtagtcg agacccgagt agcggccatt gatgcgcccc gcttcgtact ccagatcctg gcgaatcttc tgataggtgg cgacgtccga acgggtgggc ggcgcgatct gcaccagcgt gacgttgccg cgccattccg gcgagcgctc cagcagatgc tcgaaggccc ggaaccgctc gaccagtccc ttcgaatagt ccagccggtc gacgctcatg atcagcttgc gcccttccag gctctgcttc aggtcgagca cgtgctgacg gctttcgtag cgcttcgcct gctcggcgat ttcgtcgggg aacacgccga tccggtagac gccggtgcgc agcttgcggc cgaacgcttc gacgagaccg tccgcactca ccgtgccgcg cgcgtggcgc gtgacgtaat ccttgaacgc gagttcgtcg gtggcggtct ggaagcccag caggtcgtag cacgacagcg acttcaccag ttcctcgtgc ggcggaatgt tgagcaggat ctgcggcgcg ggaaacggaa tgtgcaggaa gaagccgatg cggttcgtga caccctcgtt gcgcagcgcc tcggcgaacg ggatcaggtg gtagtcgtga atccagatca cgtcgtcggg ttcgagcagc ttgatcagct tgtgcgcgag ccacgcgttg acgcgccggt agcccgtgta ttcctcgcgc tcgtagcgcg cgaggtcgtt gcggtagtgg aacaccggcc acaatgtcgc gttcgaaaag ccgcgatagt actggtcgta atccttgcgc gtgagcccta cggtcgcgaa ggtcaccgcg ccgtcctgct ccagcgtcgg accggcgttc gcgacggtct cgctgaccac gtcgccgctc cagccgaacc acacgcctcc ggcatccttc agcgcgccga atacgccgac cgcgaggccg ccggccgatc ccttggtttc cgtcggcgtt gcgacgcgat tcgacacgac gatcaatcgc cccatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMG RLIVVSNRVA TPTETKGSAG GLAVGVFGAL KDAGGVWFGW SGDVVSETVA NAGPTLEQDG AVTFATVGLT RKDYDQYYRG FSNATLWPVF HYRNDLARYE REEYTGYRRV NAWLAHKLIK LLEPDDVIWI HDYHLIPFAE ALRNEGVTNR IGFFLHIPFP APQILLNIPP HEELVKSLSC YDLLGFQTAT DELAFKDYVT RHARGTVSAD GLVEAFGRKL RTGVYRIGVF PDEIAEQAKR YESRQHVLDL KQSLEGRKLI MSVDRLDYSK GLVERFRAFE HLLERSPEWR GNVTLVQIAP PTRSDVATYQ KIRQDLEYEA GRINGRYSGL DYTPIRYLNQ QYDRWKLMSL FRESQIGFVT PLHDGMNLVA KEYVAAQNPD DPGVLVLSIF AGAAAELSGA LLVNPHDAIG MCEALQRALQ MPLDARQRRH EINMAALRKN DLGVWRDTFL HDLRSVPVQQ PGKGGGHN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGGC GATTGATCGT CGTGTCGAAT CGCGTCGCAA CGCCGACGGA AACCAAGGGA TCGGCCGGCG GCCTCGCGGT CGGCGTATTC GGCGCGCTGA AGGATGCCGG AGGCGTGTGG TTCGGCTGGA GCGGCGACGT GGTCAGCGAG ACCGTCGCGA ACGCCGGTCC GACGCTGGAG CAGGACGGCG CGGTGACCTT CGCGACCGTA GGGCTCACGC GCAAGGATTA CGACCAGTAC TATCGCGGCT TTTCGAACGC GACATTGTGG CCGGTGTTCC ACTACCGCAA CGACCTCGCG CGCTACGAGC GCGAGGAATA CACGGGCTAC CGGCGCGTCA ACGCGTGGCT CGCGCACAAG CTGATCAAGC TGCTCGAACC CGACGACGTG ATCTGGATTC ACGACTACCA CCTGATCCCG TTCGCCGAGG CGCTGCGCAA CGAGGGTGTC ACGAACCGCA TCGGCTTCTT CCTGCACATT CCGTTTCCCG CGCCGCAGAT CCTGCTCAAC ATTCCGCCGC ACGAGGAACT GGTGAAGTCG CTGTCGTGCT ACGACCTGCT GGGCTTCCAG ACCGCCACCG ACGAACTCGC GTTCAAGGAT TACGTCACGC GCCACGCGCG CGGCACGGTG AGTGCGGACG GTCTCGTCGA AGCGTTCGGC CGCAAGCTGC GCACCGGCGT CTACCGGATC GGCGTGTTCC CCGACGAAAT CGCCGAGCAG GCGAAGCGCT ACGAAAGCCG TCAGCACGTG CTCGACCTGA AGCAGAGCCT GGAAGGGCGC AAGCTGATCA TGAGCGTCGA CCGGCTGGAC TATTCGAAGG GACTGGTCGA GCGGTTCCGG GCCTTCGAGC ATCTGCTGGA GCGCTCGCCG GAATGGCGCG GCAACGTCAC GCTGGTGCAG ATCGCGCCGC CCACCCGTTC GGACGTCGCC ACCTATCAGA AGATTCGCCA GGATCTGGAG TACGAAGCGG GGCGCATCAA TGGCCGCTAC TCGGGTCTCG ACTACACGCC GATCCGCTAT CTGAATCAGC AGTACGACCG CTGGAAGCTG ATGTCGCTGT TTCGCGAATC GCAGATCGGT TTCGTCACGC CGCTGCACGA CGGTATGAAT CTGGTCGCGA AGGAATACGT CGCCGCGCAG AATCCCGACG ATCCCGGCGT GCTGGTGCTG TCGATTTTCG CGGGTGCGGC GGCGGAGCTG AGCGGCGCGC TGCTCGTCAA TCCACATGAT GCGATCGGCA TGTGCGAAGC CTTGCAGCGC GCGTTGCAGA TGCCGCTCGA CGCACGTCAG CGGCGCCATG AAATCAACAT GGCCGCGCTG CGCAAGAACG ATCTCGGCGT GTGGCGCGAC ACGTTCCTGC ACGATCTGCG CAGCGTGCCG GTGCAGCAGC CAGGCAAGGG TGGCGGGCAT AACTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT