BuxeA.00046.b
Alpha,alpha-trehalose-phosphate synthase(UDP-forming)
| CENTER ID: | BuxeA.00046.b |
| ORGANISM: | Burkholderia xenovorans LB400 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuxeA.00046.b.B1.GE39358 | Full length( BuxeA.00046.b ) | 1 | 486 | ||
| BuxeA.00046.b.B11.GE39359 | Full length( BuxeA.00046.b ) | 1 | 486 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuxeA.00046.b.B1.PS02504 | Full length( BuxeA.00046.b ) | 1 | 486 |
Structures
5V0T
DEPOSITED: 2/28/2017
DETERMINATION: XRay
CLONE:
PROTEIN: BuxeA.00046.d.B11.PS02506
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129327 |
| PATRIC ID: | fig|266265.5.peg.3333 |
| RefSeq: | YP_559763.1 |
| UniProt: | Q13W28 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGRLIIVSNR VAPISEGGPA AGGLAVGVYD ALKETGGMWF GWSGDVLSSG QPQIKVEERG PVTFATIALM RRDYDQYYRG FSNATLWPAF HYRADLLQYD RHDFEGYWRV NAWLAQQLVP LLREDDVIWV HDYHLIPFAQ ALRAAGVKNR IGFFLHIPFP ASQVLLAVPP HRELVEALCS FDLLGFQTAP DLRAFCDYIV NEANGTADPS ASGPLTIHAF GRTLRAAAYP IGVYPDEIAE LAKAGERGKP VRTMKATLHS RKLIMSVDRL DYSKGLVERF RAFERLLEHS TAQRNKVSFL QIAPPTRADM HAYQDIRLQL EGESGRINGR FAELDWTPIL YIHKQYERSV LAALFRTAHV GYVTPLRDGM NLVAKEYVSA QDPENPGVLV LSRFAGAAQE LDGALIVNPV DIDGMAEALA RALDMPLAER QARHRDMMVQ LRENNVSVWR DNFMRDLQGV AGAKGTRNGK ARAVAGKKAA RETQAG
NT Sequence
ATGGGCCGAC TGATCATCGT ATCGAACCGG GTTGCGCCGA TCTCGGAGGG CGGCCCGGCG GCGGGTGGTC TGGCGGTCGG CGTGTACGAC GCACTGAAGG AAACCGGCGG CATGTGGTTC GGCTGGAGCG GCGACGTGCT CAGTTCGGGC CAGCCGCAGA TCAAGGTCGA AGAGCGCGGC CCGGTGACCT TCGCGACGAT CGCGCTGATG CGCCGCGATT ACGATCAGTA CTACCGTGGC TTCTCGAACG CGACGCTGTG GCCCGCGTTC CACTATCGCG CCGACCTGCT GCAATACGAC CGGCACGACT TCGAGGGCTA CTGGCGCGTC AATGCCTGGC TCGCGCAACA ACTCGTGCCG CTGTTGCGCG AAGACGATGT GATCTGGGTT CACGACTATC ACCTGATTCC ATTTGCGCAG GCGCTGCGTG CGGCGGGCGT GAAGAATCGC ATCGGCTTTT TTCTGCACAT TCCGTTTCCG GCTTCCCAGG TGCTGCTGGC AGTGCCGCCG CATCGCGAAC TTGTCGAAGC GCTGTGTTCG TTCGATCTGC TCGGCTTCCA GACTGCGCCG GATTTGCGCG CGTTCTGCGA CTACATCGTC AATGAGGCGA ACGGCACCGC CGACCCATCG GCAAGCGGTC CGCTGACGAT CCACGCGTTC GGCCGCACGC TGCGGGCGGC AGCTTATCCG ATCGGCGTCT ATCCGGACGA GATCGCCGAA CTCGCGAAAG CGGGCGAGCG CGGCAAACCG GTGCGCACGA TGAAGGCGAC GCTGCACTCG CGCAAGCTGA TCATGAGCGT GGACCGGCTC GATTACTCGA AGGGGCTGGT CGAGCGTTTT CGCGCCTTCG AGCGTCTGCT CGAACACTCG ACCGCGCAAC GCAACAAGGT GTCGTTCCTG CAGATCGCGC CGCCGACGCG CGCCGACATG CACGCATACC AGGACATCCG CTTGCAGCTC GAAGGGGAGT CGGGGCGCAT CAACGGGCGC TTCGCCGAAC TGGACTGGAC GCCGATTCTC TACATCCACA AGCAATACGA GCGCTCGGTG CTGGCCGCGC TGTTCCGCAC CGCGCATGTC GGCTATGTCA CGCCGTTGCG TGACGGCATG AACCTCGTCG CCAAGGAGTA CGTATCGGCG CAGGATCCGG AGAATCCCGG CGTGCTCGTG CTGTCACGCT TTGCCGGCGC CGCGCAGGAA CTGGACGGCG CGCTGATCGT CAATCCGGTC GACATCGACG GCATGGCCGA GGCGCTCGCA CGGGCGCTCG ACATGCCGCT CGCGGAGCGT CAGGCGCGTC ATCGCGACAT GATGGTGCAG TTGCGCGAGA ACAATGTGTC GGTGTGGCGC GATAACTTCA TGCGCGATCT GCAGGGCGTC GCTGGTGCAA AGGGCACCCG GAATGGGAAG GCGCGCGCAG TGGCGGGGAA AAAGGCGGCG CGCGAAACGC AGGCCGGG
Details for BuxeA.00046.b.B1.GE39358
| HARVESTED ON: | 7/10/2015 |
| SEQUENCED ON: | 7/6/2015 |
| EXPECTED MW: | 55kDa |
| OBSERVED MW: | 55kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG RLIIVSNRVA PISEGGPAAG GLAVGVYDAL KETGGMWFGW SGDVLSSGQP QIKVEERGPV TFATIALMRR DYDQYYRGFS NATLWPAFHY RADLLQYDRH DFEGYWRVNA WLAQQLVPLL REDDVIWVHD YHLIPFAQAL RAAGVKNRIG FFLHIPFPAS QVLLAVPPHR ELVEALCSFD LLGFQTAPDL RAFCDYIVNE ANGTADPSAS GPLTIHAFGR TLRAAAYPIG VYPDEIAELA KAGERGKPVR TMKATLHSRK LIMSVDRLDY SKGLVERFRA FERLLEHSTA QRNKVSFLQI APPTRADMHA YQDIRLQLEG ESGRINGRFA ELDWTPILYI HKQYERSVLA ALFRTAHVGY VTPLRDGMNL VAKEYVSAQD PENPGVLVLS RFAGAAQELD GALIVNPVDI DGMAEALARA LDMPLAERQA RHRDMMVQLR ENNVSVWRDN FMRDLQGVAG AKGTRNGKAR AVAGKKAARE TQAG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggcc gactgatcat cgtatcgaac cgggttgcgc cgatctcgga gggcggcccg gcggcgggtg gtctggcggt cggcgtgtac gacgcactga aggaaaccgg cggcatgtgg ttcggctgga gcggcgacgt gctcagttcg ggccagccgc agatcaaggt cgaagagcgc ggcccggtga ccttcgcgac gatcgcgctg atgcgccgcg attacgatca gtactaccgt ggcttctcga acgcgacgct gtggcccgcg ttccactatc gcgccgacct gctgcaatac gaccggcacg acttcgaggg ctactggcgc gtcaatgcct ggctcgcgca acaactcgtg ccgctgttgc gcgaagacga tgtgatctgg gttcacgact atcacctgat tccatttgcg caggcgctgc gtgcggcggg cgtgaagaat cgcatcggct tttttctgca cattccgttt ccggcttccc aggtgctgct ggcagtgccg ccgcatcgcg aacttgtcga agcgctgtgt tcgttcgatc tgctcggctt ccagactgcg ccggatttgc gcgcgttctg cgactacatc gtcaatgagg cgaacggcac cgccgaccca tcggcaagcg gtccgctgac gatccacgcg ttcggccgca cgctgcgggc ggcagcttat ccgatcggcg tctatccgga cgagatcgcc gaactcgcga aagcgggcga gcgcggcaaa ccggtgcgca cgatgaaggc gacgctgcac tcgcgcaagc tgatcatgag cgtggaccgg ctcgattact cgaaggggct ggtcgagcgt tttcgcgcct tcgagcgtct gctcgaacac tcgaccgcgc aacgcaacaa ggtgtcgttc ctgcagatcg cgccgccgac gcgcgccgac atgcacgcat accaggacat ccgcttgcag ctcgaagggg agtcggggcg catcaacggg cgcttcgccg aactggactg gacgccgatt ctctacatcc acaagcaata cgagcgctcg gtgctggccg cgctgttccg caccgcgcat gtcggctatg tcacgccgtt gcgtgacggc atgaacctcg tcgccaagga gtacgtatcg gcgcaggatc cggagaatcc cggcgtgctc gtgctgtcac gctttgccgg cgccgcgcag gaactggacg gcgcgctgat cgtcaatccg gtcgacatcg acggcatggc cgaggcgctc gcacgggcgc tcgacatgcc gctcgcggag cgtcaggcgc gtcatcgcga catgatggtg cagttgcgcg agaacaatgt gtcggtgtgg cgcgataact tcatgcgcga tctgcagggc gtcgctggtg caaagggcac ccggaatggg aaggcgcgcg cagtggcggg gaaaaaggcg gcgcgcgaaa cgcaggccgg gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for BuxeA.00046.b.B11.GE39359
| HARVESTED ON: | 7/10/2015 |
| SEQUENCED ON: | 7/6/2015 |
| EXPECTED MW: | 55kDa |
| OBSERVED MW: | 55kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG RLIIVSNRVA PISEGGPAAG GLAVGVYDAL KETGGMWFGW SGDVLSSGQP QIKVEERGPV TFATIALMRR DYDQYYRGFS NATLWPAFHY RADLLQYDRH DFEGYWRVNA WLAQQLVPLL REDDVIWVHD YHLIPFAQAL RAAGVKNRIG FFLHIPFPAS QVLLAVPPHR ELVEALCSFD LLGFQTAPDL RAFCDYIVNE ANGTADPSAS GPLTIHAFGR TLRAAAYPIG VYPDEIAELA KAGERGKPVR TMKATLHSRK LIMSVDRLDY SKGLVERFRA FERLLEHSTA QRNKVSFLQI APPTRADMHA YQDIRLQLEG ESGRINGRFA ELDWTPILYI HKQYERSVLA ALFRTAHVGY VTPLRDGMNL VAKEYVSAQD PENPGVLVLS RFAGAAQELD GALIVNPVDI DGMAEALARA LDMPLAERQA RHRDMMVQLR ENNVSVWRDN FMRDLQGVAG AKGTRNGKAR AVAGKKAARE TQAG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggcc gactgatcat cgtatcgaac cgggttgcgc cgatctcgga gggcggcccg gcggcgggtg gtctggcggt cggcgtgtac gacgcactga aggaaaccgg cggcatgtgg ttcggctgga gcggcgacgt gctcagttcg ggccagccgc agatcaaggt cgaagagcgc ggcccggtga ccttcgcgac gatcgcgctg atgcgccgcg attacgatca gtactaccgt ggcttctcga acgcgacgct gtggcccgcg ttccactatc gcgccgacct gctgcaatac gaccggcacg acttcgaggg ctactggcgc gtcaatgcct ggctcgcgca acaactcgtg ccgctgttgc gcgaagacga tgtgatctgg gttcacgact atcacctgat tccatttgcg caggcgctgc gtgcggcggg cgtgaagaat cgcatcggct tttttctgca cattccgttt ccggcttccc aggtgctgct ggcagtgccg ccgcatcgcg aacttgtcga agcgctgtgt tcgttcgatc tgctcggctt ccagactgcg ccggatttgc gcgcgttctg cgactacatc gtcaatgagg cgaacggcac cgccgaccca tcggcaagcg gtccgctgac gatccacgcg ttcggccgca cgctgcgggc ggcagcttat ccgatcggcg tctatccgga cgagatcgcc gaactcgcga aagcgggcga gcgcggcaaa ccggtgcgca cgatgaaggc gacgctgcac tcgcgcaagc tgatcatgag cgtggaccgg ctcgattact cgaaggggct ggtcgagcgt tttcgcgcct tcgagcgtct gctcgaacac tcgaccgcgc aacgcaacaa ggtgtcgttc ctgcagatcg cgccgccgac gcgcgccgac atgcacgcat accaggacat ccgcttgcag ctcgaagggg agtcggggcg catcaacggg cgcttcgccg aactggactg gacgccgatt ctctacatcc acaagcaata cgagcgctcg gtgctggccg cgctgttccg caccgcgcat gtcggctatg tcacgccgtt gcgtgacggc atgaacctcg tcgccaagga gtacgtatcg gcgcaggatc cggagaatcc cggcgtgctc gtgctgtcac gctttgccgg cgccgcgcag gaactggacg gcgcgctgat cgtcaatccg gtcgacatcg acggcatggc cgaggcgctc gcacgggcgc tcgacatgcc gctcgcggag cgtcaggcgc gtcatcgcga catgatggtg cagttgcgcg agaacaatgt gtcggtgtgg cgcgataact tcatgcgcga tctgcagggc gtcgctggtg caaagggcac ccggaatggg aaggcgcgcg cagtggcggg gaaaaaggcg gcgcgcgaaa cgcaggccgg gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for BuxeA.00046.b.B1.PS02504
| PURIFICATION DATe: | 9/30/2015 |
| CONCENTRATION: | 24.97mg/ml |
| OBSERVED MW: | 55kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG RLIIVSNRVA PISEGGPAAG GLAVGVYDAL KETGGMWFGW SGDVLSSGQP QIKVEERGPV TFATIALMRR DYDQYYRGFS NATLWPAFHY RADLLQYDRH DFEGYWRVNA WLAQQLVPLL REDDVIWVHD YHLIPFAQAL RAAGVKNRIG FFLHIPFPAS QVLLAVPPHR ELVEALCSFD LLGFQTAPDL RAFCDYIVNE ANGTADPSAS GPLTIHAFGR TLRAAAYPIG VYPDEIAELA KAGERGKPVR TMKATLHSRK LIMSVDRLDY SKGLVERFRA FERLLEHSTA QRNKVSFLQI APPTRADMHA YQDIRLQLEG ESGRINGRFA ELDWTPILYI HKQYERSVLA ALFRTAHVGY VTPLRDGMNL VAKEYVSAQD PENPGVLVLS RFAGAAQELD GALIVNPVDI DGMAEALARA LDMPLAERQA RHRDMMVQLR ENNVSVWRDN FMRDLQGVAG AKGTRNGKAR AVAGKKAARE TQAG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGCC GACTGATCAT CGTATCGAAC CGGGTTGCGC CGATCTCGGA GGGCGGCCCG GCGGCGGGTG GTCTGGCGGT CGGCGTGTAC GACGCACTGA AGGAAACCGG CGGCATGTGG TTCGGCTGGA GCGGCGACGT GCTCAGTTCG GGCCAGCCGC AGATCAAGGT CGAAGAGCGC GGCCCGGTGA CCTTCGCGAC GATCGCGCTG ATGCGCCGCG ATTACGATCA GTACTACCGT GGCTTCTCGA ACGCGACGCT GTGGCCCGCG TTCCACTATC GCGCCGACCT GCTGCAATAC GACCGGCACG ACTTCGAGGG CTACTGGCGC GTCAATGCCT GGCTCGCGCA ACAACTCGTG CCGCTGTTGC GCGAAGACGA TGTGATCTGG GTTCACGACT ATCACCTGAT TCCATTTGCG CAGGCGCTGC GTGCGGCGGG CGTGAAGAAT CGCATCGGCT TTTTTCTGCA CATTCCGTTT CCGGCTTCCC AGGTGCTGCT GGCAGTGCCG CCGCATCGCG AACTTGTCGA AGCGCTGTGT TCGTTCGATC TGCTCGGCTT CCAGACTGCG CCGGATTTGC GCGCGTTCTG CGACTACATC GTCAATGAGG CGAACGGCAC CGCCGACCCA TCGGCAAGCG GTCCGCTGAC GATCCACGCG TTCGGCCGCA CGCTGCGGGC GGCAGCTTAT CCGATCGGCG TCTATCCGGA CGAGATCGCC GAACTCGCGA AAGCGGGCGA GCGCGGCAAA CCGGTGCGCA CGATGAAGGC GACGCTGCAC TCGCGCAAGC TGATCATGAG CGTGGACCGG CTCGATTACT CGAAGGGGCT GGTCGAGCGT TTTCGCGCCT TCGAGCGTCT GCTCGAACAC TCGACCGCGC AACGCAACAA GGTGTCGTTC CTGCAGATCG CGCCGCCGAC GCGCGCCGAC ATGCACGCAT ACCAGGACAT CCGCTTGCAG CTCGAAGGGG AGTCGGGGCG CATCAACGGG CGCTTCGCCG AACTGGACTG GACGCCGATT CTCTACATCC ACAAGCAATA CGAGCGCTCG GTGCTGGCCG CGCTGTTCCG CACCGCGCAT GTCGGCTATG TCACGCCGTT GCGTGACGGC ATGAACCTCG TCGCCAAGGA GTACGTATCG GCGCAGGATC CGGAGAATCC CGGCGTGCTC GTGCTGTCAC GCTTTGCCGG CGCCGCGCAG GAACTGGACG GCGCGCTGAT CGTCAATCCG GTCGACATCG ACGGCATGGC CGAGGCGCTC GCACGGGCGC TCGACATGCC GCTCGCGGAG CGTCAGGCGC GTCATCGCGA CATGATGGTG CAGTTGCGCG AGAACAATGT GTCGGTGTGG CGCGATAACT TCATGCGCGA TCTGCAGGGC GTCGCTGGTG CAAAGGGCAC CCGGAATGGG AAGGCGCGCG CAGTGGCGGG GAAAAAGGCG GCGCGCGAAA CGCAGGCCGG GTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT