BuxeA.00046.a

Alpha,alpha-trehalose-phosphate synthase(UDP-forming)

CENTER ID: BuxeA.00046.a
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00046.a.B1.GE39356 Full length( BuxeA.00046.a ) 1 460
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.8608
RefSeq: YP_556074.1
UniProt: Q13GR5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRLVVVSNR TVDPRKTAAG GLAVAVRESL QRTGGLWFGW SGKIYDESDE MTSRRGVHVQ TLGNVTLATV DLSQQDHDNY YLGYANGVLW PVFHYRLDLA DFDTRFAAGY RRVNQLFAEK LLPLLRPDDI IWVHDYHLIP LAAELRAIGC GNRIGFFLHI PMPPPMIMAA IPEHEWLMRS LFAYDLVGFQ GQIDVEHFTH YAESEANARQ VGPQQLRAFN RTLRLGAYPI GIDVEEFVSL ANGKEAREMF TRMRDEYSRR RLLLGVDRLD YSKGLPQRIH AFRELLAHYP ENRDSATLIQ IASPSRESVA AYDDLRRQMD SLCGAINGDY GELDWMPVRY IHRHVARKRL PGLYRASKVA LVTPLRDGMN LVAKEFIAAQ DPSDPGMLVL SRFAGAAEQL KEALLVNPYD TQGTAHAIQR ALTMPLDERR NRHAALMKRI REHDVHWWSS SFLDTLQDIP
NT Sequence
ATGAGTCGCC TGGTTGTGGT CTCAAATCGT ACGGTCGACC CACGTAAAAC TGCTGCCGGC GGCCTCGCTG TCGCTGTGCG GGAAAGTCTT CAGCGCACCG GTGGCCTTTG GTTCGGCTGG AGCGGGAAAA TCTACGACGA GTCGGATGAG ATGACAAGCC GGCGTGGCGT TCACGTGCAA ACACTCGGCA ATGTCACACT CGCCACAGTC GATCTGAGCC AGCAGGATCA TGACAACTAC TATCTCGGCT ATGCGAACGG AGTGCTGTGG CCGGTCTTTC ACTATCGGCT GGATCTCGCG GACTTCGACA CGCGCTTCGC TGCCGGTTAC CGGCGCGTGA ACCAGCTGTT TGCCGAGAAA CTCCTTCCAC TGCTCAGACC CGATGACATC ATCTGGGTGC ACGACTATCA CCTGATTCCG CTTGCAGCGG AATTACGCGC AATAGGCTGT GGTAACCGTA TCGGGTTCTT TCTGCATATT CCAATGCCGC CGCCGATGAT CATGGCAGCC ATTCCGGAAC ACGAATGGTT GATGCGTTCG TTGTTCGCCT ATGATCTCGT GGGCTTCCAG GGGCAGATCG ACGTCGAGCA CTTTACGCAT TACGCCGAAT CCGAAGCGAA CGCCAGACAG GTCGGCCCGC AACAACTCCG TGCATTCAAT CGCACATTGA GACTGGGTGC GTATCCCATC GGAATCGATG TGGAGGAGTT CGTCAGTCTC GCCAATGGCA AGGAAGCGCG CGAGATGTTC ACGCGGATGC GTGACGAGTA TTCGCGTCGA CGCCTGCTGC TAGGCGTCGA CCGGCTCGAT TACTCCAAAG GCTTGCCTCA GCGCATTCAC GCGTTTCGAG AACTGCTTGC ACACTATCCA GAGAATCGCG ATAGCGCGAC CTTGATCCAG ATTGCGTCGC CTAGCCGCGA AAGCGTGGCT GCCTATGACG ATCTTCGTCG TCAGATGGAC AGCCTGTGCG GCGCGATCAA CGGCGACTAC GGTGAACTGG ACTGGATGCC GGTTCGCTAC ATTCACCGGC ACGTGGCACG CAAGCGCCTG CCTGGTCTTT ATCGCGCGAG CAAGGTCGCG CTGGTCACTC CGCTGCGCGA CGGGATGAAT CTCGTCGCCA AGGAATTTAT CGCCGCACAG GATCCGTCTG ATCCCGGCAT GTTGGTACTG TCCCGTTTCG CCGGCGCCGC GGAGCAACTC AAGGAAGCGT TGCTGGTCAA TCCCTACGAC ACGCAAGGCA CCGCGCATGC CATCCAAAGG GCGCTGACCA TGCCGCTCGA TGAACGTCGC AATCGTCATG CTGCACTGAT GAAAAGGATT CGCGAGCATG ACGTCCACTG GTGGTCTTCC AGTTTCCTGG ACACACTCCA GGATATTCCT
Details for BuxeA.00046.a.B1.GE39356
HARVESTED ON: 7/10/2015
SEQUENCED ON: 7/6/2015
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS RLVVVSNRTV DPRKTAAGGL AVAVRESLQR TGGLWFGWSG KIYDESDEMT SRRGVHVQTL GNVTLATVDL SQQDHDNYYL GYANGVLWPV FHYRLDLADF DTRFAAGYRR VNQLFAEKLL PLLRPDDIIW VHDYHLIPLA AELRAIGCGN RIGFFLHIPM PPPMIMAAIP EHEWLMRSLF AYDLVGFQGQ IDVEHFTHYA ESEANARQVG PQQLRAFNRT LRLGAYPIGI DVEEFVSLAN GKEAREMFTR MRDEYSRRRL LLGVDRLDYS KGLPQRIHAF RELLAHYPEN RDSATLIQIA SPSRESVAAY DDLRRQMDSL CGAINGDYGE LDWMPVRYIH RHVARKRLPG LYRASKVALV TPLRDGMNLV AKEFIAAQDP SDPGMLVLSR FAGAAEQLKE ALLVNPYDTQ GTAHAIQRAL TMPLDERRNR HAALMKRIRE HDVHWWSSSF LDTLQDIP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtc gcctggttgt ggtctcaaat cgtacggtcg acccacgtaa aactgctgcc ggcggcctcg ctgtcgctgt gcgggaaagt cttcagcgca ccggtggcct ttggttcggc tggagcggga aaatctacga cgagtcggat gagatgacaa gccggcgtgg cgttcacgtg caaacactcg gcaatgtcac actcgccaca gtcgatctga gccagcagga tcatgacaac tactatctcg gctatgcgaa cggagtgctg tggccggtct ttcactatcg gctggatctc gcggacttcg acacgcgctt cgctgccggt taccggcgcg tgaaccagct gtttgccgag aaactccttc cactgctcag acccgatgac atcatctggg tgcacgacta tcacctgatt ccgcttgcag cggaattacg cgcaataggc tgtggtaacc gtatcgggtt ctttctgcat attccaatgc cgccgccgat gatcatggca gccattccgg aacacgaatg gttgatgcgt tcgttgttcg cctatgatct cgtgggcttc caggggcaga tcgacgtcga gcactttacg cattacgccg aatccgaagc gaacgccaga caggtcggcc cgcaacaact ccgtgcattc aatcgcacat tgagactggg tgcgtatccc atcggaatcg atgtggagga gttcgtcagt ctcgccaatg gcaaggaagc gcgcgagatg ttcacgcgga tgcgtgacga gtattcgcgt cgacgcctgc tgctaggcgt cgaccggctc gattactcca aaggcttgcc tcagcgcatt cacgcgtttc gagaactgct tgcacactat ccagagaatc gcgatagcgc gaccttgatc cagattgcgt cgcctagccg cgaaagcgtg gctgcctatg acgatcttcg tcgtcagatg gacagcctgt gcggcgcgat caacggcgac tacggtgaac tggactggat gccggttcgc tacattcacc ggcacgtggc acgcaagcgc ctgcctggtc tttatcgcgc gagcaaggtc gcgctggtca ctccgctgcg cgacgggatg aatctcgtcg ccaaggaatt tatcgccgca caggatccgt ctgatcccgg catgttggta ctgtcccgtt tcgccggcgc cgcggagcaa ctcaaggaag cgttgctggt caatccctac gacacgcaag gcaccgcgca tgccatccaa agggcgctga ccatgccgct cgatgaacgt cgcaatcgtc atgctgcact gatgaaaagg attcgcgagc atgacgtcca ctggtggtct tccagtttcc tggacacact ccaggatatt ccttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat