SSGCID: Target Page

BuxeA.00039.a

Methionine aminopeptidase

CENTER ID:	BuxeA.00039.a
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00039.a.B1.GE39354	Full length( BuxeA.00039.a )		1	255

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.7530
RefSeq:	YP_555027.1
UniProt:	Q13JR2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTKRPEEIAL MAESGQLLAD VFGYLDQMSL IGMSTMQVND LVDSLIVNEL NACPASKGQY GYAYALNSSR NNVVCHGVPS TTDILQSGDI VNFDITLEKN GYIADSSKTY LVGEVSPLAK RLVQVTYEAM WKGIKTVRPG ARLGDVGHAI ERHARRNGYS VVREYCGHGI GREMHEEPQV LHWGKPRTGL LLREGMVFTI EPMINQGRHS VRTEEDGWTV VTRDGQLSAQ FEHTVAVTRN GVQVLTLRSE ERVPN

NT Sequence

ATGACCAAGC GACCCGAAGA AATCGCATTG ATGGCGGAAT CGGGCCAACT GCTGGCAGAT GTATTTGGCT ATCTGGATCA AATGAGTCTG ATCGGCATGT CGACCATGCA GGTCAACGAT CTGGTCGATA GCCTCATCGT CAATGAACTC AACGCATGCC CGGCAAGCAA AGGCCAGTAC GGCTACGCCT ACGCGCTTAA TTCTTCACGC AACAATGTTG TTTGTCACGG TGTTCCGTCC ACCACAGACA TCCTGCAAAG CGGGGACATC GTTAATTTCG ACATCACGCT GGAGAAGAAT GGCTACATCG CTGATTCCAG TAAGACCTAT CTCGTGGGGG AGGTGTCTCC ACTGGCTAAA CGCCTCGTGC AAGTGACCTA CGAAGCGATG TGGAAAGGGA TCAAGACCGT TCGTCCCGGC GCCAGACTCG GCGATGTAGG TCATGCTATT GAGCGGCACG CGCGCAGAAA TGGCTATTCG GTCGTGAGGG AATACTGCGG GCATGGCATC GGGCGTGAAA TGCATGAGGA GCCGCAAGTG CTGCATTGGG GGAAGCCGCG AACGGGTTTG TTGTTGCGCG AAGGTATGGT GTTCACCATT GAACCCATGA TCAACCAGGG GCGACATTCT GTTAGAACCG AAGAAGATGG CTGGACGGTG GTCACGCGCG ACGGGCAACT GTCGGCGCAG TTCGAACACA CCGTGGCCGT GACCCGAAAC GGCGTACAGG TCTTAACGCT ACGATCCGAA GAAAGGGTAC CCAAC

Details for BuxeA.00039.a.B1.GE39354

HARVESTED ON:	7/10/2015
SEQUENCED ON:	7/6/2015
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT KRPEEIALMA ESGQLLADVF GYLDQMSLIG MSTMQVNDLV DSLIVNELNA CPASKGQYGY AYALNSSRNN VVCHGVPSTT DILQSGDIVN FDITLEKNGY IADSSKTYLV GEVSPLAKRL VQVTYEAMWK GIKTVRPGAR LGDVGHAIER HARRNGYSVV REYCGHGIGR EMHEEPQVLH WGKPRTGLLL REGMVFTIEP MINQGRHSVR TEEDGWTVVT RDGQLSAQFE HTVAVTRNGV QVLTLRSEER VPN

Validated NT Sequence

atcttactca cttagttggg taccctttct tcggatcgta gcgttaagac ctgtacgccg tttcgggtca cggccacggt gtgttcgaac tgcgccgaca gttgcccgtc gcgcgtgacc accgtccagc catcttcttc ggttctaaca gaatgtcgcc cctggttgat catgggttca atggtgaaca ccataccttc gcgcaacaac aaacccgttc gcggcttccc ccaatgcagc acttgcggct cctcatgcat ttcacgcccg atgccatgcc cgcagtattc cctcacgacc gaatagccat ttctgcgcgc gtgccgctca atagcatgac ctacatcgcc gagtctggcg ccgggacgaa cggtcttgat ccctttccac atcgcttcgt aggtcacttg cacgaggcgt ttagccagtg gagacacctc ccccacgaga taggtcttac tggaatcagc gatgtagcca ttcttctcca gcgtgatgtc gaaattaacg atgtccccgc tttgcaggat gtctgtggtg gacggaacac cgtgacaaac aacattgttg cgtgaagaat taagcgcgta ggcgtagccg tactggcctt tgcttgccgg gcatgcgttg agttcattga cgatgaggct atcgaccaga tcgttgacct gcatggtcga catgccgatc agactcattt gatccagata gccaaataca tctgccagca gttggcccga ttccgccatc aatgcgattt cttcgggtcg cttggtcata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacca agcgacccga agaaatcgca ttgatggcgg aatcgggcca actgctggca gatgtatttg gctatctgga tcaaatgagt ctgatcggca tgtcgaccat gcaggtcaac gatctggtcg atagcctcat cgtcaatgaa ctcaacgcat gcccggcaag caaaggccag tacggctacg cctacgcgct taattcttca cgcaacaatg ttgtttgtca cggtgttccg tccaccacag acatcctgca aagcggggac atcgttaatt tcgacatcac gctggagaag aatggctaca tcgctgattc cagtaagacc tatctcgtgg gggaggtgtc tccactggct aaacgcctcg tgcaagtgac ctacgaagcg atgtggaaag ggatcaagac cgttcgtccc ggcgccagac tcggcgatgt aggtcatgct attgagcggc acgcgcgcag aaatggctat tcggtcgtga gggaatactg cgggcatggc atcgggcgtg aaatgcatga ggagccgcaa gtgctgcatt gggggaagcc gcgaacgggt ttgttgttgc gcgaaggtat ggtgttcacc attgaaccca tgatcaacca ggggcgacat tctgttagaa ccgaagaaga tggctggacg gtggtcacgc gcgacgggca actgtcggcg cagttcgaac acaccgtggc cgtgacccga aacggcgtac aggtcttaac gctacgatcc gaagaaaggg tacccaactg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat