BuxeA.00036.a
Phosphoribosylaminoimidazole carboxylase
| CENTER ID: | BuxeA.00036.a |
| ORGANISM: | Burkholderia xenovorans LB400 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuxeA.00036.a.A1.GE32987 | Full length( BuxeA.00036.a ) | 1 | 398 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuxeA.00036.a.A1.PW33597 | Full length( BuxeA.00036.a ) | 1 | 398 | ||
| BuxeA.00036.a.B2.PW39468 | AlphaFold domain | 9 | 397 |
Structures
9ZOI
DEPOSITED: 12/15/2025
DETERMINATION: XRay
CLONE: BuxeA.00036.a.B2.GE45405
PROTEIN: BuxeA.00036.a.B2.PW39468
9ZOK
DEPOSITED: 12/15/2025
DETERMINATION: XRay
CLONE: BuxeA.00036.a.B2.GE45405
PROTEIN: BuxeA.00036.a.B2.PW39468
9ZPB
DEPOSITED: 12/16/2025
DETERMINATION: XRay
CLONE: BuxeA.00036.a.B2.GE45405
PROTEIN: BuxeA.00036.a.B2.PW39468
10BM
DEPOSITED: 1/9/2026
DETERMINATION: XRay
CLONE: BuxeA.00036.a.B2.GE45405
PROTEIN: BuxeA.00036.a.B2.PW39468
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|266265.5.peg.3909 |
| RefSeq: | YP_560292.1 |
| UniProt: | Q13UJ9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNPDNNPVSP ILPGAWLGMV GGGQLGRMFC FAAQAMGYRV AVLDPDENSP AGAVADRHLR AAYNDEASLT ELARLCAAVS TEFENVPAAS LDFLARTTFV SPAGRCVAVA QDRIAEKRFI ASSGVTVAPH VVIESSDALA ALDDAKLEAV LPGILKTARM GYDGKGQIRV RNAEEVREAH ASLAGVPCVL EKRLPLKFEV SALIARAASG ASVVYPLAQN THRDGVLSHT IVPAPDASPT LVQQAQQAAL QIADKLGYVG VLCVEFFILE DGSLVANEMA PRPHNSGHYT VDACATSQFE QQVRAMTGMP LGDTRQHSPA VMLNILGDVW FPDGPKRPAV TPPWQEVAAM PAARLHLYGK EEARCGRKMG HVNFTAATLE EARTAARDCA RLLHIITS
NT Sequence
ATGAACCCAG ACAACAACCC GGTTTCACCG ATTCTGCCCG GCGCATGGCT TGGCATGGTC GGTGGCGGCC AGCTCGGCCG CATGTTCTGC TTTGCCGCTC AGGCTATGGG CTATCGCGTC GCCGTGCTCG ATCCCGACGA AAACAGTCCG GCGGGCGCCG TTGCCGATCG GCATCTGCGC GCGGCGTACA ACGACGAAGC GTCGCTCACC GAGCTCGCGC GGCTGTGCGC GGCGGTGTCC ACGGAGTTCG AGAACGTGCC TGCCGCGAGC CTCGACTTCC TCGCGCGGAC CACCTTCGTG AGTCCGGCTG GCCGCTGCGT CGCCGTGGCG CAGGACCGCA TTGCCGAGAA GCGCTTCATT GCGTCATCGG GCGTGACGGT CGCGCCGCAT GTGGTGATCG AATCGTCGGA TGCGCTCGCC GCGCTCGACG ACGCCAAACT CGAAGCCGTG CTGCCGGGCA TTCTCAAGAC CGCCCGCATG GGCTACGACG GCAAAGGCCA GATTCGCGTG CGCAACGCCG AGGAAGTGCG CGAGGCGCAT GCTTCGCTCG CCGGAGTGCC GTGCGTGCTG GAAAAGCGGT TGCCGCTGAA GTTCGAAGTG TCCGCGCTGA TTGCGCGGGC AGCGAGCGGC GCCTCGGTGG TCTATCCGCT CGCGCAGAAC ACGCATCGCG ACGGCGTGCT GTCGCACACC ATTGTCCCGG CGCCGGACGC GAGCCCGACG CTCGTTCAGC AGGCGCAGCA GGCCGCGCTG CAAATCGCCG ACAAACTCGG TTACGTGGGC GTGCTGTGCG TCGAGTTTTT CATTCTTGAA GACGGCTCGC TGGTCGCCAA TGAAATGGCG CCGCGTCCGC ACAACTCCGG CCATTACACG GTCGACGCCT GCGCGACCAG CCAGTTCGAG CAGCAGGTGC GCGCCATGAC CGGCATGCCG TTGGGCGATA CACGTCAGCA TTCGCCGGCG GTGATGCTCA ACATTCTCGG CGACGTGTGG TTTCCGGACG GCCCGAAGCG CCCGGCGGTC ACGCCGCCCT GGCAGGAGGT CGCCGCGATG CCGGCGGCGC GTTTGCATCT GTACGGCAAG GAAGAGGCGC GTTGCGGCCG CAAGATGGGC CACGTGAACT TCACGGCGGC TACGCTCGAA GAAGCCCGCA CCGCTGCGCG CGATTGCGCA CGGCTTCTGC ACATCATCAC GAGC
Details for BuxeA.00036.a.A1.GE32987
| HARVESTED ON: | 8/16/2011 |
| SEQUENCED ON: | 8/22/2011 |
| EXPECTED MW: | 44kDa |
| OBSERVED MW: | 44kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Validated AA Sequence
MNPDNNPVSP ILPGAWLGMV GGGQLGRMFC FAAQAMGYRV AVLDPDENSP AGAVADRHLR AAYNDEASLT ELARLCAAVS TEFENVPAAS LDFLARTTFV SPAGRCVAVA QDRIAEKRFI ASSGVTVAPH VVIESSDALA ALDDAKLEAV LPGILKTARM GYDGKGQIRV RNAEEVREAH ASLAGVPCVL EKRLPLKFEV SALIARAASG ASVVYPLAQN THRDGVLSHT IVPAPDASPT LVQQAQQAAL QIADKLGYVG VLCVEFFILE DGSLVANEMA PRPHNSGHYT VDACATSQFE QQVRAMTGMP LGDTRQHSPA VMLNILGDVW FPDGPKRPAV TPPWQEVAAM PAARLHLYGK EEARCGRKMG HVNFTAATLE EARTAARDCA RLLHIIT
Validated NT Sequence
gctcgtgatg atgtgcagaa gccgtgcgca atcgcgcgca gcggtgcggg cttcttcgag cgtagccgcc gtgaagttca cgtggcccat cttgcggccg caacgcgcct cttccttgcc gtacagatgc aaacgcgccg ccggcatcgc ggcgacctcc tgccagggcg gcgtgaccgc cgggcgcttc gggccgtccg gaaaccacac gtcgccgaga atgttgagca tcaccgccgg cgaatgctga cgtgtatcgc ccaacggcat gccggtcatg gcgcgcacct gctgctcgaa ctggctggtc gcgcaggcgt cgaccgtgta atggccggag ttgtgcggac gcggcgccat ttcattggcg accagcgagc cgtcttcaag aatgaaaaac tcgacgcaca gcacgcccac gtaaccgagt ttgtcggcga tttgcagcgc ggcctgctgc gcctgctgaa cgagcgtcgg gctcgcgtcc ggcgccggga caatggtgtg cgacagcacg ccgtcgcgat gcgtgttctg cgcgagcgga tagaccaccg aggcgccgct cgctgcccgc gcaatcagcg cggacacttc gaacttcagc ggcaaccgct tttccagcac gcacggcact ccggcgagcg aagcatgcgc ctcgcgcact tcctcggcgt tgcgcacgcg aatctggcct ttgccgtcgt agcccatgcg ggcggtcttg agaatgcccg gcagcacggc ttcgagtttg gcgtcgtcga gcgcggcgag cgcatccgac gattcgatca ccacatgcgg cgcgaccgtc acgcccgatg acgcaatgaa gcgcttctcg gcaatgcggt cctgcgccac ggcgacgcag cggccagccg gactcacgaa ggtggtccgc gcgaggaagt cgaggctcgc ggcaggcacg ttctcgaact ccgtggacac cgccgcgcac agccgcgcga gctcggtgag cgacgcttcg tcgttgtacg ccgcgcgcag atgccgatcg gcaacggcgc ccgccggact gttttcgtcg ggatcgagca cggcgacgcg atagcccata gcctgagcgg caaagcagaa catgcggccg agctggccgc caccgaccat gccaagccat gcgccgggca gaatcggtga aaccgggttg ttgtctgggt tcatcgaacc aggaccctgg gtctgagctt ccaggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNPDNNPVS PILPGAWLGM VGGGQLGRMF CFAAQAMGYR VAVLDPDENS PAGAVADRHL RAAYNDEASL TELARLCAAV STEFENVPAA SLDFLARTTF VSPAGRCVAV AQDRIAEKRF IASSGVTVAP HVVIESSDAL AALDDAKLEA VLPGILKTAR MGYDGKGQIR VRNAEEVREA HASLAGVPCV LEKRLPLKFE VSALIARAAS GASVVYPLAQ NTHRDGVLSH TIVPAPDASP TLVQQAQQAA LQIADKLGYV GVLCVEFFIL EDGSLVANEM APRPHNSGHY TVDACATSQF EQQVRAMTGM PLGDTRQHSP AVMLNILGDV WFPDGPKRPA VTPPWQEVAA MPAARLHLYG KEEARCGRKM GHVNFTAATL EEARTAARDC ARLLHIITS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacccagac aacaacccgg tttcaccgat tctgcccggc gcatggcttg gcatggtcgg tggcggccag ctcggccgca tgttctgctt tgccgctcag gctatgggct atcgcgtcgc cgtgctcgat cccgacgaaa acagtccggc gggcgccgtt gccgatcggc atctgcgcgc ggcgtacaac gacgaagcgt cgctcaccga gctcgcgcgg ctgtgcgcgg cggtgtccac ggagttcgag aacgtgcctg ccgcgagcct cgacttcctc gcgcggacca ccttcgtgag tccggctggc cgctgcgtcg ccgtggcgca ggaccgcatt gccgagaagc gcttcattgc gtcatcgggc gtgacggtcg cgccgcatgt ggtgatcgaa tcgtcggatg cgctcgccgc gctcgacgac gccaaactcg aagccgtgct gccgggcatt ctcaagaccg cccgcatggg ctacgacggc aaaggccaga ttcgcgtgcg caacgccgag gaagtgcgcg aggcgcatgc ttcgctcgcc ggagtgccgt gcgtgctgga aaagcggttg ccgctgaagt tcgaagtgtc cgcgctgatt gcgcgggcag cgagcggcgc ctcggtggtc tatccgctcg cgcagaacac gcatcgcgac ggcgtgctgt cgcacaccat tgtcccggcg ccggacgcga gcccgacgct cgttcagcag gcgcagcagg ccgcgctgca aatcgccgac aaactcggtt acgtgggcgt gctgtgcgtc gagtttttca ttcttgaaga cggctcgctg gtcgccaatg aaatggcgcc gcgtccgcac aactccggcc attacacggt cgacgcctgc gcgaccagcc agttcgagca gcaggtgcgc gccatgaccg gcatgccgtt gggcgataca cgtcagcatt cgccggcggt gatgctcaac attctcggcg acgtgtggtt tccggacggc ccgaagcgcc cggcggtcac gccgccctgg caggaggtcg ccgcgatgcc ggcggcgcgt ttgcatctgt acggcaagga agaggcgcgt tgcggccgca agatgggcca cgtgaacttc acggcggcta cgctcgaaga agcccgcacc gctgcgcgcg attgcgcacg gcttctgcac atcatcacga gctaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BuxeA.00036.a.A1.PW33597
| PURIFICATION DATe: | 11/11/2011 |
| CONCENTRATION: | 32.78mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Protocol Notes
notes unavailable
Validated AA Sequence
MNPDNNPVSP ILPGAWLGMV GGGQLGRMFC FAAQAMGYRV AVLDPDENSP AGAVADRHLR AAYNDEASLT ELARLCAAVS TEFENVPAAS LDFLARTTFV SPAGRCVAVA QDRIAEKRFI ASSGVTVAPH VVIESSDALA ALDDAKLEAV LPGILKTARM GYDGKGQIRV RNAEEVREAH ASLAGVPCVL EKRLPLKFEV SALIARAASG ASVVYPLAQN THRDGVLSHT IVPAPDASPT LVQQAQQAAL QIADKLGYVG VLCVEFFILE DGSLVANEMA PRPHNSGHYT VDACATSQFE QQVRAMTGMP LGDTRQHSPA VMLNILGDVW FPDGPKRPAV TPPWQEVAAM PAARLHLYGK EEARCGRKMG HVNFTAATLE EARTAARDCA RLLHIIT
Validated NT Sequence
gctcgtgatg atgtgcagaa gccgtgcgca atcgcgcgca gcggtgcggg cttcttcgag cgtagccgcc gtgaagttca cgtggcccat cttgcggccg caacgcgcct cttccttgcc gtacagatgc aaacgcgccg ccggcatcgc ggcgacctcc tgccagggcg gcgtgaccgc cgggcgcttc gggccgtccg gaaaccacac gtcgccgaga atgttgagca tcaccgccgg cgaatgctga cgtgtatcgc ccaacggcat gccggtcatg gcgcgcacct gctgctcgaa ctggctggtc gcgcaggcgt cgaccgtgta atggccggag ttgtgcggac gcggcgccat ttcattggcg accagcgagc cgtcttcaag aatgaaaaac tcgacgcaca gcacgcccac gtaaccgagt ttgtcggcga tttgcagcgc ggcctgctgc gcctgctgaa cgagcgtcgg gctcgcgtcc ggcgccggga caatggtgtg cgacagcacg ccgtcgcgat gcgtgttctg cgcgagcgga tagaccaccg aggcgccgct cgctgcccgc gcaatcagcg cggacacttc gaacttcagc ggcaaccgct tttccagcac gcacggcact ccggcgagcg aagcatgcgc ctcgcgcact tcctcggcgt tgcgcacgcg aatctggcct ttgccgtcgt agcccatgcg ggcggtcttg agaatgcccg gcagcacggc ttcgagtttg gcgtcgtcga gcgcggcgag cgcatccgac gattcgatca ccacatgcgg cgcgaccgtc acgcccgatg acgcaatgaa gcgcttctcg gcaatgcggt cctgcgccac ggcgacgcag cggccagccg gactcacgaa ggtggtccgc gcgaggaagt cgaggctcgc ggcaggcacg ttctcgaact ccgtggacac cgccgcgcac agccgcgcga gctcggtgag cgacgcttcg tcgttgtacg ccgcgcgcag atgccgatcg gcaacggcgc ccgccggact gttttcgtcg ggatcgagca cggcgacgcg atagcccata gcctgagcgg caaagcagaa catgcggccg agctggccgc caccgaccat gccaagccat gcgccgggca gaatcggtga aaccgggttg ttgtctgggt tcatcgaacc aggaccctgg gtctgagctt ccaggg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNPDNNPVS PILPGAWLGM VGGGQLGRMF CFAAQAMGYR VAVLDPDENS PAGAVADRHL RAAYNDEASL TELARLCAAV STEFENVPAA SLDFLARTTF VSPAGRCVAV AQDRIAEKRF IASSGVTVAP HVVIESSDAL AALDDAKLEA VLPGILKTAR MGYDGKGQIR VRNAEEVREA HASLAGVPCV LEKRLPLKFE VSALIARAAS GASVVYPLAQ NTHRDGVLSH TIVPAPDASP TLVQQAQQAA LQIADKLGYV GVLCVEFFIL EDGSLVANEM APRPHNSGHY TVDACATSQF EQQVRAMTGM PLGDTRQHSP AVMLNILGDV WFPDGPKRPA VTPPWQEVAA MPAARLHLYG KEEARCGRKM GHVNFTAATL EEARTAARDC ARLLHIITS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAACCCAGAC AACAACCCGG TTTCACCGAT TCTGCCCGGC GCATGGCTTG GCATGGTCGG TGGCGGCCAG CTCGGCCGCA TGTTCTGCTT TGCCGCTCAG GCTATGGGCT ATCGCGTCGC CGTGCTCGAT CCCGACGAAA ACAGTCCGGC GGGCGCCGTT GCCGATCGGC ATCTGCGCGC GGCGTACAAC GACGAAGCGT CGCTCACCGA GCTCGCGCGG CTGTGCGCGG CGGTGTCCAC GGAGTTCGAG AACGTGCCTG CCGCGAGCCT CGACTTCCTC GCGCGGACCA CCTTCGTGAG TCCGGCTGGC CGCTGCGTCG CCGTGGCGCA GGACCGCATT GCCGAGAAGC GCTTCATTGC GTCATCGGGC GTGACGGTCG CGCCGCATGT GGTGATCGAA TCGTCGGATG CGCTCGCCGC GCTCGACGAC GCCAAACTCG AAGCCGTGCT GCCGGGCATT CTCAAGACCG CCCGCATGGG CTACGACGGC AAAGGCCAGA TTCGCGTGCG CAACGCCGAG GAAGTGCGCG AGGCGCATGC TTCGCTCGCC GGAGTGCCGT GCGTGCTGGA AAAGCGGTTG CCGCTGAAGT TCGAAGTGTC CGCGCTGATT GCGCGGGCAG CGAGCGGCGC CTCGGTGGTC TATCCGCTCG CGCAGAACAC GCATCGCGAC GGCGTGCTGT CGCACACCAT TGTCCCGGCG CCGGACGCGA GCCCGACGCT CGTTCAGCAG GCGCAGCAGG CCGCGCTGCA AATCGCCGAC AAACTCGGTT ACGTGGGCGT GCTGTGCGTC GAGTTTTTCA TTCTTGAAGA CGGCTCGCTG GTCGCCAATG AAATGGCGCC GCGTCCGCAC AACTCCGGCC ATTACACGGT CGACGCCTGC GCGACCAGCC AGTTCGAGCA GCAGGTGCGC GCCATGACCG GCATGCCGTT GGGCGATACA CGTCAGCATT CGCCGGCGGT GATGCTCAAC ATTCTCGGCG ACGTGTGGTT TCCGGACGGC CCGAAGCGCC CGGCGGTCAC GCCGCCCTGG CAGGAGGTCG CCGCGATGCC GGCGGCGCGT TTGCATCTGT ACGGCAAGGA AGAGGCGCGT TGCGGCCGCA AGATGGGCCA CGTGAACTTC ACGGCGGCTA CGCTCGAAGA AGCCCGCACC GCTGCGCGCG ATTGCGCACG GCTTCTGCAC ATCATCACGA GCTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA
Details for BuxeA.00036.a.B2.PW39468
| PURIFICATION DATe: | 8/26/2025 |
| CONCENTRATION: | 23.8mg/ml |
| OBSERVED MW: | 42kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 15 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHSP ILPGAWLGMV GGGQLGRMFC FAAQAMGYRV AVLDPDENSP AGAVADRHLR AAYNDEASLT ELARLCAAVS TEFENVPAAS LDFLARTTFV SPAGRCVAVA QDRIAEKRFI ASSGVTVAPH VVIESSDALA ALDDAKLEAV LPGILKTARM GYDGKGQIRV RNAEEVREAH ASLAGVPCVL EKRLPLKFEV SALIARAASG ASVVYPLAQN THRDGVLSHT IVPAPDASPT LVQQAQQAAL QIADKLGYVG VLCVEFFILE DGSLVANEMA PRPHNSGHYT VDACATSQFE QQVRAMTGMP LGDTRQHSPA VMLNILGDVW FPDGPKRPAV TPPWQEVAAM PAARLHLYGK EEARCGRKMG HVNFTAATLE EARTAARDCA RLLHIIT
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTCACCGA TTCTGCCCGG CGCATGGCTT GGCATGGTCG GTGGCGGCCA GCTCGGCCGC ATGTTCTGCT TTGCCGCTCA GGCTATGGGC TATCGCGTCG CCGTGCTCGA TCCCGACGAA AACAGTCCGG CGGGCGCCGT TGCCGATCGG CATCTGCGCG CGGCGTACAA CGACGAAGCG TCGCTCACCG AGCTCGCGCG GCTGTGCGCG GCGGTGTCCA CGGAGTTCGA GAACGTGCCT GCCGCGAGCC TCGACTTCCT CGCGCGGACC ACCTTCGTGA GTCCGGCTGG CCGCTGCGTC GCCGTGGCGC AGGACCGCAT TGCCGAGAAG CGCTTCATTG CGTCATCGGG CGTGACGGTC GCGCCGCATG TGGTGATCGA ATCGTCGGAT GCGCTCGCCG CGCTCGACGA CGCCAAACTC GAAGCCGTGC TGCCGGGCAT TCTCAAGACC GCCCGCATGG GCTACGACGG CAAAGGCCAG ATTCGCGTGC GCAACGCCGA GGAAGTGCGC GAGGCGCATG CTTCGCTCGC CGGAGTGCCG TGCGTGCTGG AAAAGCGGTT GCCGCTGAAG TTCGAAGTGT CCGCGCTGAT TGCGCGGGCA GCGAGCGGCG CCTCGGTGGT CTATCCGCTC GCGCAGAACA CGCATCGCGA CGGCGTGCTG TCGCACACCA TTGTCCCGGC GCCGGACGCG AGCCCGACGC TCGTTCAGCA GGCGCAGCAG GCCGCGCTGC AAATCGCCGA CAAACTCGGT TACGTGGGCG TGCTGTGCGT CGAGTTTTTC ATTCTTGAAG ACGGCTCGCT GGTCGCCAAT GAAATGGCGC CGCGTCCGCA CAACTCCGGC CATTACACGG TCGACGCCTG CGCGACCAGC CAGTTCGAGC AGCAGGTGCG CGCCATGACC GGCATGCCGT TGGGCGATAC ACGTCAGCAT TCGCCGGCGG TGATGCTCAA CATTCTCGGC GACGTGTGGT TTCCGGACGG CCCGAAGCGC CCGGCGGTCA CGCCGCCCTG GCAGGAGGTC GCCGCGATGC CGGCGGCGCG TTTGCATCTG TACGGCAAGG AAGAGGCGCG TTGCGGCCGC AAGATGGGCC ACGTGAACTT CACGGCGGCT ACGCTCGAAG AAGCCCGCAC CGCTGCGCGC GATTGCGCAC GGCTTCTGCA CATCATCACG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT