BuxeA.00027.q

Putative acyl-CoA dehydrogenase

CENTER ID: BuxeA.00027.q
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00027.q.B1.GE39438 Full length( BuxeA.00027.q ) 1 416
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.3179
RefSeq: YP_559616.1
UniProt: Q13WH5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRAPPASLYP EQPVMDLNFT PEEEAFRADV QAFLRDRLPP RLADKVHGGR RLTRDDMAGW HAILNAQGWL ANHWPKEYGG PGWNPVQKFI FENECALAGA PRVVPFGVNM LGPVLIKYGS EAQKRHWLPR ILDGSDWWCQ GYSEPGAGSD LASVKTSAVR GTDAQGEHYI VNGQKTWTTL GHYANMIFCL VRSATDVRKQ EGISFLLIDM KTPGVEVCPI ITLDGEHEVN EVFFTDVRVP VENLVGEENK GWTYAKYLLT YERTNIAGVG FSVAAFNRLR KIAAKQRRNG RPLAEDPAFA ARMARVEIDL ENMKTTNLRV IAAVAGGGVP GAESSMLKIR GTEIRQEISS LTRRAMGPYA QPFVEEALQD GFEGTPVGPD EAASAASLYF NNRKLSIFGG SNEIQKNIIS KMILGL
NT Sequence
TTGCGCGCGC CGCCCGCCTC ACTCTATCCG GAACAACCCG TCATGGATCT GAACTTCACT CCCGAGGAAG AGGCTTTCCG CGCCGACGTG CAGGCCTTCT TGCGTGACAG GCTCCCGCCG CGACTCGCCG ACAAGGTGCA CGGCGGCCGC CGTCTGACGC GCGACGACAT GGCCGGCTGG CACGCGATTC TCAACGCGCA AGGCTGGCTT GCGAACCACT GGCCGAAAGA ATACGGCGGC CCGGGTTGGA ACCCGGTGCA GAAATTCATC TTCGAAAACG AATGTGCGCT GGCCGGCGCG CCGCGCGTGG TGCCGTTCGG CGTCAACATG CTCGGCCCCG TGCTGATCAA ATACGGCAGC GAAGCGCAAA AACGCCATTG GCTGCCGCGT ATTCTCGACG GCTCGGACTG GTGGTGCCAG GGCTATTCGG AACCGGGCGC CGGCTCCGAT CTCGCCTCGG TCAAAACTTC CGCGGTGCGC GGCACGGATG CACAGGGCGA GCACTACATC GTCAACGGCC AGAAGACGTG GACCACGCTC GGCCACTACG CGAACATGAT TTTCTGCCTC GTACGCAGCG CCACCGACGT GCGCAAACAG GAAGGCATCA GCTTTCTGCT GATCGACATG AAGACGCCGG GCGTCGAAGT GTGCCCGATC ATCACGCTCG ACGGCGAGCA CGAAGTCAAC GAGGTGTTCT TTACCGACGT GCGCGTGCCG GTCGAGAACC TGGTCGGCGA AGAGAATAAA GGCTGGACGT ACGCCAAGTA CCTGCTCACG TATGAGCGCA CCAATATCGC GGGCGTTGGT TTTTCCGTGG CCGCGTTCAA TCGTCTGCGC AAGATTGCCG CGAAACAGCG GCGCAATGGC CGGCCGCTCG CGGAGGATCC TGCGTTCGCG GCGCGCATGG CGCGTGTCGA AATCGATCTG GAGAACATGA AGACCACCAA CCTGCGCGTG ATCGCGGCGG TGGCCGGCGG CGGCGTGCCG GGAGCCGAGA GTTCGATGCT GAAGATTCGC GGCACCGAGA TCCGTCAGGA AATCTCGTCG CTCACGCGCC GCGCAATGGG TCCGTACGCG CAACCGTTCG TCGAGGAAGC GTTGCAAGAC GGCTTCGAAG GCACGCCGGT CGGCCCCGAC GAAGCCGCGA GCGCCGCTTC GCTTTACTTC AATAATCGCA AGCTGTCGAT CTTCGGCGGC TCGAACGAAA TCCAGAAGAA CATCATTTCC AAAATGATCC TGGGACTG
Details for BuxeA.00027.q.B1.GE39438
HARVESTED ON: data unavailable
SEQUENCED ON: 8/13/2015
EXPECTED MW: 47kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: None
TOTAL EXPRESSION LEVEL: Data Unavailable
SOLUBLE EXPRESSION LEVEL Data Unavailable
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMR APPASLYPEQ PVMDLNFTPE EEAFRADVQA FLRDRLPPRL ADKVHGGRRL TRDDMAGWHA ILNAQGWLAN HWPKEYGGPG WNPVQKFIFE NECALAGAPR VVPFGVNMLG PVLIKYGSEA QKRHWLPRIL DGSDWWCQGY SEPGAGSDLA SVKTSAVRGT DAQGEHYIVN GQKTWTTLGH YANMIFCLVR SATDVRKQEG ISFLLIDMKT PGVEVCPIIT LDGEHEVNEV FFTDVRVPVE NLVGEENKGW TYAKYLLTYE RTNIAGVGFS VAAFNRLRKI AAKQRRNGRP LAEDPAFAAR MARVEIDLEN MKTTNLRVIA AVAGGGVPGA ESSMLKIRGT EIRQEISSLT RRAMGPYAQP FVEEALQDGF EGTPVGPDEA ASAASLYFNN RKLSIFGGSN EIQKNIISKM ILGL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgcgcg cgccgcccgc ctcactctat ccggaacaac ccgtcatgga tctgaacttc actcccgagg aagaggcttt ccgcgccgac gtgcaggcct tcttgcgtga caggctcccg ccgcgactcg ccgacaaggt gcacggcggc cgccgtctga cgcgcgacga catggccggc tggcacgcga ttctcaacgc gcaaggctgg cttgcgaacc actggccgaa agaatacggc ggcccgggtt ggaacccggt gcagaaattc atcttcgaaa acgaatgtgc gctggccggc gcgccgcgcg tggtgccgtt cggcgtcaac atgctcggcc ccgtgctgat caaatacggc agcgaagcgc aaaaacgcca ttggctgccg cgtattctcg acggctcgga ctggtggtgc cagggctatt cggaaccggg cgccggctcc gatctcgcct cggtcaaaac ttccgcggtg cgcggcacgg atgcacaggg cgagcactac atcgtcaacg gccagaagac gtggaccacg ctcggccact acgcgaacat gattttctgc ctcgtacgca gcgccaccga cgtgcgcaaa caggaaggca tcagctttct gctgatcgac atgaagacgc cgggcgtcga agtgtgcccg atcatcacgc tcgacggcga gcacgaagtc aacgaggtgt tctttaccga cgtgcgcgtg ccggtcgaga acctggtcgg cgaagagaat aaaggctgga cgtacgccaa gtacctgctc acgtatgagc gcaccaatat cgcgggcgtt ggtttttccg tggccgcgtt caatcgtctg cgcaagattg ccgcgaaaca gcggcgcaat ggccggccgc tcgcggagga tcctgcgttc gcggcgcgca tggcgcgtgt cgaaatcgat ctggagaaca tgaagaccac caacctgcgc gtgatcgcgg cggtggccgg cggcggcgtg ccgggagccg agagttcgat gctgaagatt cgcggcaccg agatccgtca ggaaatctcg tcgctcacgc gccgcgcaat gggtccgtac gcgcaaccgt tcgtcgagga agcgttgcaa gacggcttcg aaggcacgcc ggtcggcccc gacgaagccg cgagcgccgc ttcgctttac ttcaataatc gcaagctgtc gatcttcggc ggctcgaacg aaatccagaa gaacatcatt tccaaaatga tcctgggact gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat