BuxeA.00027.j
Putative acyl-CoA dehydrogenase
| CENTER ID: | BuxeA.00027.j |
| ORGANISM: | Burkholderia xenovorans LB400 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuxeA.00027.j.B1.GE39436 | Full length( BuxeA.00027.j ) | 1 | 402 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuxeA.00027.j.B1.PS37826 | Full length( BuxeA.00027.j ) | 1 | 402 |
Structures
5JSC
DEPOSITED: 5/7/2016
DETERMINATION: XRay
CLONE: BuxeA.00027.j.B1.GE39436
PROTEIN: BuxeA.00027.j.B1.PS37826
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|266265.5.peg.7520 |
| RefSeq: | YP_555018.1 |
| UniProt: | Q13JS1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSANTELDLH SALAWPFFEP RHRELAAGIE AWCRANLAHE DHEDVDATCR RLVRELGAAG WLKYGVGGVA YGGHGDTIDT RAVCLLRETL AKHSGLADFA LAMQGLGSGA ISLGGTHEQK TRYLPRVANG TAIAAFALSE PEAGSDVAAM TLSAREDGDA YVLDGDKTWI SNGGIADFYV VFARTGEAPG ARGISAFVVD ADTPGLEIAE RIDVIAPHPL ARLHFAGARV PRSQMLGAPG EGFKLAMRTL DIFRTSVAAA SLGFARHAMA EGVARAASRK MFGQTLGDFQ LTQAKLAQMA LTIDSSALLV YRAAWLRDQG ENVTREAAMA KWHASEGAQQ VIDAAVQLYG GMGVQSGTAV EMLYREIRAL RIYEGATEVQ QLIVGRDLLK AHAAATAGAN TR
NT Sequence
TTGAGCGCCA ACACCGAACT GGATCTGCAT AGCGCGCTGG CCTGGCCGTT CTTCGAGCCG CGCCATCGCG AACTGGCCGC GGGTATCGAA GCATGGTGCC GCGCGAACCT CGCGCACGAA GACCACGAAG ACGTGGACGC CACCTGCCGC CGTCTGGTGC GCGAACTCGG CGCGGCGGGC TGGCTGAAAT ACGGCGTGGG CGGTGTAGCC TACGGCGGCC ACGGCGACAC GATCGACACG CGCGCCGTCT GCCTGCTGCG CGAAACGCTC GCCAAACACT CTGGCCTCGC GGACTTCGCG CTGGCGATGC AAGGCCTCGG TTCGGGCGCC ATCTCGCTTG GCGGCACGCA CGAACAGAAG ACGCGCTATC TGCCGCGCGT CGCCAACGGC ACGGCCATCG CGGCGTTCGC GCTATCCGAG CCCGAGGCGG GATCGGACGT CGCCGCCATG ACGCTCTCCG CACGCGAAGA CGGCGACGCC TACGTGCTCG ACGGCGACAA GACGTGGATC TCCAACGGCG GCATCGCCGA TTTCTACGTG GTGTTCGCGA GAACGGGCGA AGCGCCGGGC GCACGCGGCA TCAGCGCGTT CGTTGTGGAT GCGGATACGC CGGGGTTGGA GATTGCGGAA CGCATCGACG TGATCGCGCC GCATCCGCTC GCACGTCTGC ACTTTGCCGG CGCGCGCGTG CCGCGCAGCC AGATGCTCGG CGCACCCGGC GAAGGTTTCA AGCTCGCCAT GCGCACGCTG GATATTTTCC GCACGTCGGT GGCGGCTGCT TCGCTTGGCT TCGCGCGTCA TGCAATGGCC GAAGGTGTCG CGCGTGCCGC GTCGCGCAAA ATGTTCGGGC AGACGCTCGG CGATTTTCAA CTGACGCAGG CCAAACTCGC GCAGATGGCC TTGACCATCG ACAGCAGCGC ATTGCTGGTC TATCGCGCGG CATGGCTGCG CGATCAAGGC GAAAACGTCA CGCGTGAAGC GGCAATGGCG AAATGGCACG CAAGCGAAGG CGCGCAACAG GTGATCGACG CCGCCGTGCA ACTCTACGGC GGCATGGGCG TGCAAAGCGG CACGGCCGTC GAAATGCTGT ATCGCGAGAT TCGCGCGCTG CGCATTTACG AAGGCGCGAC CGAAGTGCAG CAACTGATCG TCGGACGTGA TCTGCTGAAA GCGCATGCTG CCGCGACTGC CGGAGCAAAC ACCAGA
Details for BuxeA.00027.j.B1.GE39436
| HARVESTED ON: | 8/5/2015 |
| SEQUENCED ON: | 8/13/2015 |
| EXPECTED MW: | 44kDa |
| OBSERVED MW: | 44kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHML SANTELDLHS ALAWPFFEPR HRELAAGIEA WCRANLAHED HEDVDATCRR LVRELGAAGW LKYGVGGVAY GGHGDTIDTR AVCLLRETLA KHSGLADFAL AMQGLGSGAI SLGGTHEQKT RYLPRVANGT AIAAFALSEP EAGSDVAAMT LSAREDGDAY VLDGDKTWIS NGGIADFYVV FARTGEAPGA RGISAFVVDA DTPGLEIAER IDVIAPHPLA RLHFAGARVP RSQMLGAPGE GFKLAMRTLD IFRTSVAAAS LGFARHAMAE GVARAASRKM FGQTLGDFQL TQAKLAQMAL TIDSSALLVY RAAWLRDQGE NVTREAAMAK WHASEGAQQV IDAAVQLYGG MGVQSGTAVE MLYREIRALR IYEGATEVQQ LIVGRDLLKA HAA
Validated NT Sequence
cggcagcatg cgctttcagc agatcacgtc cgacgatcag ttgctgcact tcggtcgcgc cttcgtaaat gcgcagcgcg cgaatctcgc gatacagcat ttcgacggcc gtgccgcttt gcacgcccat gccgccgtag agttgcacgg cggcgtcgat cacctgttgc gcgccttcgc ttgcgtgcca tttcgccatt gccgcttcac gcgtgacgtt ttcgccttga tcgcgcagcc atgccgcgcg atagaccagc aatgcgctgc tgtcgatggt caaggccatc tgcgcgagtt tggcctgcgt cagttgaaaa tcgccgagcg tctgcccgaa cattttgcgc gacgcggcac gcgcgacacc ttcggccatt gcatgacgcg cgaagccaag cgaagcagcc gccaccgacg tgcggaaaat atccagcgtg cgcatggcga gcttgaaacc ttcgccgggt gcgccgagca tctggctgcg cggcacgcgc gcgccggcaa agtgcagacg tgcgagcgga tgcggcgcga tcacgtcgat gcgttccgca atctccaacc ccggcgtatc cgcatccaca acgaacgcgc tgatgccgcg tgcgcccggc gcttcgcccg ttctcgcgaa caccacgtag aaatcggcga tgccgccgtt ggagatccac gtcttgtcgc cgtcgagcac gtaggcgtcg ccgtcttcgc gtgcggagag cgtcatggcg gcgacgtccg atcccgcctc gggctcggat agcgcgaacg ccgcgatggc cgtgccgttg gcgacgcgcg gcagatagcg cgtcttctgt tcgtgcgtgc cgccaagcga gatggcgccc gaaccgaggc cttgcatcgc cagcgcgaag tccgcgaggc cagagtgttt ggcgagcgtt tcgcgcagca ggcagacggc gcgcgtgtcg atcgtgtcgc cgtggccgcc gtaggctaca ccgcccacgc cgtatttcag ccagcccgcc gcgccgagtt cgcgcaccag acggcggcag gtggcgtcca cgtcttcgtg gtcttcgtgc gcgaggttcg cgcggcacca tgcttcgata cccgcggcca gttcgcgatg gcgcggctcg aagaacggcc aggccagcgc gctatgcaga tccagttcgg tgttggcgct caacatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMS ANTELDLHSA LAWPFFEPRH RELAAGIEAW CRANLAHEDH EDVDATCRRL VRELGAAGWL KYGVGGVAYG GHGDTIDTRA VCLLRETLAK HSGLADFALA MQGLGSGAIS LGGTHEQKTR YLPRVANGTA IAAFALSEPE AGSDVAAMTL SAREDGDAYV LDGDKTWISN GGIADFYVVF ARTGEAPGAR GISAFVVDAD TPGLEIAERI DVIAPHPLAR LHFAGARVPR SQMLGAPGEG FKLAMRTLDI FRTSVAAASL GFARHAMAEG VARAASRKMF GQTLGDFQLT QAKLAQMALT IDSSALLVYR AAWLRDQGEN VTREAAMAKW HASEGAQQVI DAAVQLYGGM GVQSGTAVEM LYREIRALRI YEGATEVQQL IVGRDLLKAH AAATAGANTR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgagcg ccaacaccga actggatctg catagcgcgc tggcctggcc gttcttcgag ccgcgccatc gcgaactggc cgcgggtatc gaagcatggt gccgcgcgaa cctcgcgcac gaagaccacg aagacgtgga cgccacctgc cgccgtctgg tgcgcgaact cggcgcggcg ggctggctga aatacggcgt gggcggtgta gcctacggcg gccacggcga cacgatcgac acgcgcgccg tctgcctgct gcgcgaaacg ctcgccaaac actctggcct cgcggacttc gcgctggcga tgcaaggcct cggttcgggc gccatctcgc ttggcggcac gcacgaacag aagacgcgct atctgccgcg cgtcgccaac ggcacggcca tcgcggcgtt cgcgctatcc gagcccgagg cgggatcgga cgtcgccgcc atgacgctct ccgcacgcga agacggcgac gcctacgtgc tcgacggcga caagacgtgg atctccaacg gcggcatcgc cgatttctac gtggtgttcg cgagaacggg cgaagcgccg ggcgcacgcg gcatcagcgc gttcgttgtg gatgcggata cgccggggtt ggagattgcg gaacgcatcg acgtgatcgc gccgcatccg ctcgcacgtc tgcactttgc cggcgcgcgc gtgccgcgca gccagatgct cggcgcaccc ggcgaaggtt tcaagctcgc catgcgcacg ctggatattt tccgcacgtc ggtggcggct gcttcgcttg gcttcgcgcg tcatgcaatg gccgaaggtg tcgcgcgtgc cgcgtcgcgc aaaatgttcg ggcagacgct cggcgatttt caactgacgc aggccaaact cgcgcagatg gccttgacca tcgacagcag cgcattgctg gtctatcgcg cggcatggct gcgcgatcaa ggcgaaaacg tcacgcgtga agcggcaatg gcgaaatggc acgcaagcga aggcgcgcaa caggtgatcg acgccgccgt gcaactctac ggcggcatgg gcgtgcaaag cggcacggcc gtcgaaatgc tgtatcgcga gattcgcgcg ctgcgcattt acgaaggcgc gaccgaagtg cagcaactga tcgtcggacg tgatctgctg aaagcgcatg ctgccgcgac tgccggagca aacaccagat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BuxeA.00027.j.B1.PS37826
| PURIFICATION DATe: | 2/4/2016 |
| CONCENTRATION: | 55mg/ml |
| OBSERVED MW: | 46kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | sec buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML SANTELDLHS ALAWPFFEPR HRELAAGIEA WCRANLAHED HEDVDATCRR LVRELGAAGW LKYGVGGVAY GGHGDTIDTR AVCLLRETLA KHSGLADFAL AMQGLGSGAI SLGGTHEQKT RYLPRVANGT AIAAFALSEP EAGSDVAAMT LSAREDGDAY VLDGDKTWIS NGGIADFYVV FARTGEAPGA RGISAFVVDA DTPGLEIAER IDVIAPHPLA RLHFAGARVP RSQMLGAPGE GFKLAMRTLD IFRTSVAAAS LGFARHAMAE GVARAASRKM FGQTLGDFQL TQAKLAQMAL TIDSSALLVY RAAWLRDQGE NVTREAAMAK WHASEGAQQV IDAAVQLYGG MGVQSGTAVE MLYREIRALR IYEGATEVQQ LIVGRDLLKA HAA
Validated NT Sequence
cggcagcatg cgctttcagc agatcacgtc cgacgatcag ttgctgcact tcggtcgcgc cttcgtaaat gcgcagcgcg cgaatctcgc gatacagcat ttcgacggcc gtgccgcttt gcacgcccat gccgccgtag agttgcacgg cggcgtcgat cacctgttgc gcgccttcgc ttgcgtgcca tttcgccatt gccgcttcac gcgtgacgtt ttcgccttga tcgcgcagcc atgccgcgcg atagaccagc aatgcgctgc tgtcgatggt caaggccatc tgcgcgagtt tggcctgcgt cagttgaaaa tcgccgagcg tctgcccgaa cattttgcgc gacgcggcac gcgcgacacc ttcggccatt gcatgacgcg cgaagccaag cgaagcagcc gccaccgacg tgcggaaaat atccagcgtg cgcatggcga gcttgaaacc ttcgccgggt gcgccgagca tctggctgcg cggcacgcgc gcgccggcaa agtgcagacg tgcgagcgga tgcggcgcga tcacgtcgat gcgttccgca atctccaacc ccggcgtatc cgcatccaca acgaacgcgc tgatgccgcg tgcgcccggc gcttcgcccg ttctcgcgaa caccacgtag aaatcggcga tgccgccgtt ggagatccac gtcttgtcgc cgtcgagcac gtaggcgtcg ccgtcttcgc gtgcggagag cgtcatggcg gcgacgtccg atcccgcctc gggctcggat agcgcgaacg ccgcgatggc cgtgccgttg gcgacgcgcg gcagatagcg cgtcttctgt tcgtgcgtgc cgccaagcga gatggcgccc gaaccgaggc cttgcatcgc cagcgcgaag tccgcgaggc cagagtgttt ggcgagcgtt tcgcgcagca ggcagacggc gcgcgtgtcg atcgtgtcgc cgtggccgcc gtaggctaca ccgcccacgc cgtatttcag ccagcccgcc gcgccgagtt cgcgcaccag acggcggcag gtggcgtcca cgtcttcgtg gtcttcgtgc gcgaggttcg cgcggcacca tgcttcgata cccgcggcca gttcgcgatg gcgcggctcg aagaacggcc aggccagcgc gctatgcaga tccagttcgg tgttggcgct caacatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHMS ANTELDLHSA LAWPFFEPRH RELAAGIEAW CRANLAHEDH EDVDATCRRL VRELGAAGWL KYGVGGVAYG GHGDTIDTRA VCLLRETLAK HSGLADFALA MQGLGSGAIS LGGTHEQKTR YLPRVANGTA IAAFALSEPE AGSDVAAMTL SAREDGDAYV LDGDKTWISN GGIADFYVVF ARTGEAPGAR GISAFVVDAD TPGLEIAERI DVIAPHPLAR LHFAGARVPR SQMLGAPGEG FKLAMRTLDI FRTSVAAASL GFARHAMAEG VARAASRKMF GQTLGDFQLT QAKLAQMALT IDSSALLVYR AAWLRDQGEN VTREAAMAKW HASEGAQQVI DAAVQLYGGM GVQSGTAVEM LYREIRALRI YEGATEVQQL IVGRDLLKAH AAATAGANTR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTGAGCG CCAACACCGA ACTGGATCTG CATAGCGCGC TGGCCTGGCC GTTCTTCGAG CCGCGCCATC GCGAACTGGC CGCGGGTATC GAAGCATGGT GCCGCGCGAA CCTCGCGCAC GAAGACCACG AAGACGTGGA CGCCACCTGC CGCCGTCTGG TGCGCGAACT CGGCGCGGCG GGCTGGCTGA AATACGGCGT GGGCGGTGTA GCCTACGGCG GCCACGGCGA CACGATCGAC ACGCGCGCCG TCTGCCTGCT GCGCGAAACG CTCGCCAAAC ACTCTGGCCT CGCGGACTTC GCGCTGGCGA TGCAAGGCCT CGGTTCGGGC GCCATCTCGC TTGGCGGCAC GCACGAACAG AAGACGCGCT ATCTGCCGCG CGTCGCCAAC GGCACGGCCA TCGCGGCGTT CGCGCTATCC GAGCCCGAGG CGGGATCGGA CGTCGCCGCC ATGACGCTCT CCGCACGCGA AGACGGCGAC GCCTACGTGC TCGACGGCGA CAAGACGTGG ATCTCCAACG GCGGCATCGC CGATTTCTAC GTGGTGTTCG CGAGAACGGG CGAAGCGCCG GGCGCACGCG GCATCAGCGC GTTCGTTGTG GATGCGGATA CGCCGGGGTT GGAGATTGCG GAACGCATCG ACGTGATCGC GCCGCATCCG CTCGCACGTC TGCACTTTGC CGGCGCGCGC GTGCCGCGCA GCCAGATGCT CGGCGCACCC GGCGAAGGTT TCAAGCTCGC CATGCGCACG CTGGATATTT TCCGCACGTC GGTGGCGGCT GCTTCGCTTG GCTTCGCGCG TCATGCAATG GCCGAAGGTG TCGCGCGTGC CGCGTCGCGC AAAATGTTCG GGCAGACGCT CGGCGATTTT CAACTGACGC AGGCCAAACT CGCGCAGATG GCCTTGACCA TCGACAGCAG CGCATTGCTG GTCTATCGCG CGGCATGGCT GCGCGATCAA GGCGAAAACG TCACGCGTGA AGCGGCAATG GCGAAATGGC ACGCAAGCGA AGGCGCGCAA CAGGTGATCG ACGCCGCCGT GCAACTCTAC GGCGGCATGG GCGTGCAAAG CGGCACGGCC GTCGAAATGC TGTATCGCGA GATTCGCGCG CTGCGCATTT ACGAAGGCGC GACCGAAGTG CAGCAACTGA TCGTCGGACG TGATCTGCTG AAAGCGCATG CTGCCGCGAC TGCCGGAGCA AACACCAGAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT