BuxeA.00027.d

Putative acyl-CoA dehydrogenase

CENTER ID: BuxeA.00027.d
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00027.d.B1.GE39435 Full length( BuxeA.00027.d ) 1 429
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00027.d.B1.PS37829 Full length( BuxeA.00027.d ) 1 429

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.8378
RefSeq: YP_555865.1
UniProt: Q13HC4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSWDFETDAD FQAELDWIDQ FVREEVEPLE HVLGSPWNIH DPAFKALVKP LQQRVKERRL WACHLGPELG GPGYGQVKLA LMNEILGRAL FAPIVFGCQA PDSGNAEILA HYGTEAQKER FLKPLLAGDI VSCFAMTEPQ GGADPKVFTT RAVRDGDAWV INGRKWFASN ARYADFFIVM AITDPEVSAY KGMSMFIVPA NTPGLSIIRN VGMADEPEAT HAYLAFDDVR VADEQMLGAP GDAFVVAQVR LGGGRVHHAM RTIGLAQKAL DALCERALSR HTQGGALADK QMVQEKIADS WLELEQFRLL VMRTAWRIDR YQDYQKVRKD IAAVKAAMPR VLHDIAARAL HVHGSIGASS EMPFAGMISR AFQMGLADGP TEVHKVTVAK QLLRDYAPCE QMFPAYHRPT AAAAARRKFA AALAQGAAR
NT Sequence
ATGTCCTGGG ACTTCGAAAC CGACGCCGAT TTTCAGGCCG AACTCGACTG GATCGACCAG TTCGTGCGCG AGGAGGTCGA GCCGCTCGAA CACGTGCTGG GCAGCCCGTG GAATATCCAC GATCCGGCCT TCAAGGCGCT CGTCAAGCCG TTGCAGCAGC GGGTCAAGGA GCGCCGGCTG TGGGCCTGTC ACCTCGGCCC CGAACTGGGC GGCCCCGGCT ATGGACAGGT GAAGCTCGCG CTCATGAACG AGATTCTGGG CCGCGCGCTG TTCGCGCCGA TCGTGTTCGG CTGCCAGGCG CCGGACTCAG GCAACGCGGA AATCCTCGCG CATTACGGCA CGGAGGCGCA GAAGGAGCGC TTCCTGAAAC CATTGCTCGC CGGCGACATC GTGTCCTGCT TCGCGATGAC CGAGCCGCAG GGCGGCGCCG ACCCCAAGGT GTTCACGACC CGCGCGGTGC GCGACGGCGA TGCGTGGGTG ATCAACGGAC GCAAGTGGTT CGCGTCGAAT GCGCGCTACG CGGACTTCTT CATCGTGATG GCGATCACCG ATCCGGAAGT GTCGGCGTAC AAGGGCATGT CGATGTTCAT CGTGCCGGCG AACACGCCCG GCCTGAGCAT CATCCGCAAT GTCGGCATGG CCGACGAGCC GGAGGCGACA CACGCGTACC TGGCGTTCGA TGACGTGCGC GTGGCGGACG AACAGATGCT GGGCGCGCCG GGCGACGCGT TTGTCGTCGC GCAGGTGCGG CTTGGCGGCG GCCGGGTGCA TCATGCGATG CGCACGATCG GACTCGCGCA GAAGGCGCTC GATGCGCTAT GCGAGCGCGC GCTGTCGCGC CATACACAGG GCGGCGCGCT CGCCGACAAG CAGATGGTGC AGGAGAAGAT CGCCGACTCC TGGCTCGAAC TCGAACAGTT TCGTCTGCTC GTGATGCGGA CCGCGTGGCG CATCGACCGT TACCAGGACT ATCAGAAGGT GCGCAAGGAC ATTGCCGCCG TGAAGGCCGC GATGCCCAGG GTATTGCACG ACATTGCCGC GCGCGCGCTG CATGTGCATG GGTCGATCGG AGCCTCGTCG GAGATGCCGT TCGCGGGAAT GATCTCGCGT GCGTTTCAGA TGGGGCTGGC CGATGGGCCG ACCGAGGTTC ACAAGGTGAC GGTGGCGAAG CAACTGCTGC GCGACTACGC GCCATGCGAG CAGATGTTTC CCGCCTACCA TCGCCCGACG GCCGCCGCCG CCGCACGCCG GAAATTCGCG GCGGCGCTCG CTCAGGGAGC GGCACGA
Details for BuxeA.00027.d.B1.GE39435
HARVESTED ON: 8/5/2015
SEQUENCED ON: 8/13/2015
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS WDFETDADFQ AELDWIDQFV REEVEPLEHV LGSPWNIHDP AFKALVKPLQ QRVKERRLWA CHLGPELGGP GYGQVKLALM NEILGRALFA PIVFGCQAPD SGNAEILAHY GTEAQKERFL KPLLAGDIVS CFAMTEPQGG ADPKVFTTRA VRDGDAWVIN GRKWFASNAR YADFFIVMAI TDPEVSAYKG MSMFIVPANT PGLSIIRNVG MADEPEATHA YLAFDDVRVA DEQMLGAPGD AFVVAQVRLG GGRVHHAMRT IGLAQKALDA LCERALSRHT QGGALADKQM VQEKIADSWL ELEQFRLLVM RTAWRIDRYQ DYQKVRKDIA AVKAAMPRVL HDIAARALHV HGSIGASSEM PFAGMISRAF QMGLADGPTE VHKVTVAKQL LRDYAPCEQM FPAYHRPTAA AAARRKFAA
Validated NT Sequence
ccgccgcgaa tttccggcgt gcggcggcgg cggccgtcgg gcgatggtag gcgggaaaca tctgctcgca tggcgcgtag tcgcgcagca gttgcttcgc caccgtcacc ttgtgaacct cggtcggccc atcggccagc cccatctgaa acgcacgcga gatcattccc gcgaacggca tctccgacga ggctccgatc gacccatgca catgcagcgc gcgcgcggca atgtcgtgca ataccctggg catcgcggcc ttcacggcgg caatgtcctt gcgcaccttc tgatagtcct ggtaacggtc gatgcgccac gcggtccgca tcacgagcag acgaaactgt tcgagttcga gccaggagtc ggcgatcttc tcctgcacca tctgcttgtc ggcgagcgcg ccgccctgtg tatggcgcga cagcgcgcgc tcgcatagcg catcgagcgc cttctgcgcg agtccgatcg tgcgcatcgc atgatgcacc cggccgccgc caagccgcac ctgcgcgacg acaaacgcgt cgcccggcgc gcccagcatc tgttcgtccg ccacgcgcac gtcatcgaac gccaggtacg cgtgtgtcgc ctccggctcg tcggccatgc cgacattgcg gatgatgctc aggccgggcg tgttcgccgg cacgatgaac atcgacatgc ccttgtacgc cgacacttcc ggatcggtga tcgccatcac gatgaagaag tccgcgtagc gcgcattcga cgcgaaccac ttgcgtccgt tgatcaccca cgcatcgccg tcgcgcaccg cgcgggtcgt gaacaccttg gggtcggcgc cgccctgcgg ctcggtcatc gcgaagcagg acacgatgtc gccggcgagc aatggtttca ggaagcgctc cttctgcgcc tccgtgccgt aatgcgcgag gatttccgcg ttgcctgagt ccggcgcctg gcagccgaac acgatcggcg cgaacagcgc gcggcccaga atctcgttca tgagcgcgag cttcacctgt ccatagccgg ggccgcccag ttcggggccg aggtgacagg cccacagccg gcgctccttg acccgctgct gcaacggctt gacgagcgcc ttgaaggccg gatcgtggat attccacggg ctgcccagca cgtgttcgag cggctcgacc tcctcgcgca cgaactggtc gatccagtcg agttcggcct gaaaatcggc gtcggtttcg aagtcccagg acatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMS WDFETDADFQ AELDWIDQFV REEVEPLEHV LGSPWNIHDP AFKALVKPLQ QRVKERRLWA CHLGPELGGP GYGQVKLALM NEILGRALFA PIVFGCQAPD SGNAEILAHY GTEAQKERFL KPLLAGDIVS CFAMTEPQGG ADPKVFTTRA VRDGDAWVIN GRKWFASNAR YADFFIVMAI TDPEVSAYKG MSMFIVPANT PGLSIIRNVG MADEPEATHA YLAFDDVRVA DEQMLGAPGD AFVVAQVRLG GGRVHHAMRT IGLAQKALDA LCERALSRHT QGGALADKQM VQEKIADSWL ELEQFRLLVM RTAWRIDRYQ DYQKVRKDIA AVKAAMPRVL HDIAARALHV HGSIGASSEM PFAGMISRAF QMGLADGPTE VHKVTVAKQL LRDYAPCEQM FPAYHRPTAA AAARRKFAAA LAQGAAR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcct gggacttcga aaccgacgcc gattttcagg ccgaactcga ctggatcgac cagttcgtgc gcgaggaggt cgagccgctc gaacacgtgc tgggcagccc gtggaatatc cacgatccgg ccttcaaggc gctcgtcaag ccgttgcagc agcgggtcaa ggagcgccgg ctgtgggcct gtcacctcgg ccccgaactg ggcggccccg gctatggaca ggtgaagctc gcgctcatga acgagattct gggccgcgcg ctgttcgcgc cgatcgtgtt cggctgccag gcgccggact caggcaacgc ggaaatcctc gcgcattacg gcacggaggc gcagaaggag cgcttcctga aaccattgct cgccggcgac atcgtgtcct gcttcgcgat gaccgagccg cagggcggcg ccgaccccaa ggtgttcacg acccgcgcgg tgcgcgacgg cgatgcgtgg gtgatcaacg gacgcaagtg gttcgcgtcg aatgcgcgct acgcggactt cttcatcgtg atggcgatca ccgatccgga agtgtcggcg tacaagggca tgtcgatgtt catcgtgccg gcgaacacgc ccggcctgag catcatccgc aatgtcggca tggccgacga gccggaggcg acacacgcgt acctggcgtt cgatgacgtg cgcgtggcgg acgaacagat gctgggcgcg ccgggcgacg cgtttgtcgt cgcgcaggtg cggcttggcg gcggccgggt gcatcatgcg atgcgcacga tcggactcgc gcagaaggcg ctcgatgcgc tatgcgagcg cgcgctgtcg cgccatacac agggcggcgc gctcgccgac aagcagatgg tgcaggagaa gatcgccgac tcctggctcg aactcgaaca gtttcgtctg ctcgtgatgc ggaccgcgtg gcgcatcgac cgttaccagg actatcagaa ggtgcgcaag gacattgccg ccgtgaaggc cgcgatgccc agggtattgc acgacattgc cgcgcgcgcg ctgcatgtgc atgggtcgat cggagcctcg tcggagatgc cgttcgcggg aatgatctcg cgtgcgtttc agatggggct ggccgatggg ccgaccgagg ttcacaaggt gacggtggcg aagcaactgc tgcgcgacta cgcgccatgc gagcagatgt ttcccgccta ccatcgcccg acggccgccg ccgccgcacg ccggaaattc gcggcggcgc tcgctcaggg agcggcacga tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BuxeA.00027.d.B1.PS37829
PURIFICATION DATe: 2/9/2016
CONCENTRATION: 39mg/ml
OBSERVED MW: 53kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS WDFETDADFQ AELDWIDQFV REEVEPLEHV LGSPWNIHDP AFKALVKPLQ QRVKERRLWA CHLGPELGGP GYGQVKLALM NEILGRALFA PIVFGCQAPD SGNAEILAHY GTEAQKERFL KPLLAGDIVS CFAMTEPQGG ADPKVFTTRA VRDGDAWVIN GRKWFASNAR YADFFIVMAI TDPEVSAYKG MSMFIVPANT PGLSIIRNVG MADEPEATHA YLAFDDVRVA DEQMLGAPGD AFVVAQVRLG GGRVHHAMRT IGLAQKALDA LCERALSRHT QGGALADKQM VQEKIADSWL ELEQFRLLVM RTAWRIDRYQ DYQKVRKDIA AVKAAMPRVL HDIAARALHV HGSIGASSEM PFAGMISRAF QMGLADGPTE VHKVTVAKQL LRDYAPCEQM FPAYHRPTAA AAARRKFAA
Validated NT Sequence
ccgccgcgaa tttccggcgt gcggcggcgg cggccgtcgg gcgatggtag gcgggaaaca tctgctcgca tggcgcgtag tcgcgcagca gttgcttcgc caccgtcacc ttgtgaacct cggtcggccc atcggccagc cccatctgaa acgcacgcga gatcattccc gcgaacggca tctccgacga ggctccgatc gacccatgca catgcagcgc gcgcgcggca atgtcgtgca ataccctggg catcgcggcc ttcacggcgg caatgtcctt gcgcaccttc tgatagtcct ggtaacggtc gatgcgccac gcggtccgca tcacgagcag acgaaactgt tcgagttcga gccaggagtc ggcgatcttc tcctgcacca tctgcttgtc ggcgagcgcg ccgccctgtg tatggcgcga cagcgcgcgc tcgcatagcg catcgagcgc cttctgcgcg agtccgatcg tgcgcatcgc atgatgcacc cggccgccgc caagccgcac ctgcgcgacg acaaacgcgt cgcccggcgc gcccagcatc tgttcgtccg ccacgcgcac gtcatcgaac gccaggtacg cgtgtgtcgc ctccggctcg tcggccatgc cgacattgcg gatgatgctc aggccgggcg tgttcgccgg cacgatgaac atcgacatgc ccttgtacgc cgacacttcc ggatcggtga tcgccatcac gatgaagaag tccgcgtagc gcgcattcga cgcgaaccac ttgcgtccgt tgatcaccca cgcatcgccg tcgcgcaccg cgcgggtcgt gaacaccttg gggtcggcgc cgccctgcgg ctcggtcatc gcgaagcagg acacgatgtc gccggcgagc aatggtttca ggaagcgctc cttctgcgcc tccgtgccgt aatgcgcgag gatttccgcg ttgcctgagt ccggcgcctg gcagccgaac acgatcggcg cgaacagcgc gcggcccaga atctcgttca tgagcgcgag cttcacctgt ccatagccgg ggccgcccag ttcggggccg aggtgacagg cccacagccg gcgctccttg acccgctgct gcaacggctt gacgagcgcc ttgaaggccg gatcgtggat attccacggg ctgcccagca cgtgttcgag cggctcgacc tcctcgcgca cgaactggtc gatccagtcg agttcggcct gaaaatcggc gtcggtttcg aagtcccagg acatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHMS WDFETDADFQ AELDWIDQFV REEVEPLEHV LGSPWNIHDP AFKALVKPLQ QRVKERRLWA CHLGPELGGP GYGQVKLALM NEILGRALFA PIVFGCQAPD SGNAEILAHY GTEAQKERFL KPLLAGDIVS CFAMTEPQGG ADPKVFTTRA VRDGDAWVIN GRKWFASNAR YADFFIVMAI TDPEVSAYKG MSMFIVPANT PGLSIIRNVG MADEPEATHA YLAFDDVRVA DEQMLGAPGD AFVVAQVRLG GGRVHHAMRT IGLAQKALDA LCERALSRHT QGGALADKQM VQEKIADSWL ELEQFRLLVM RTAWRIDRYQ DYQKVRKDIA AVKAAMPRVL HDIAARALHV HGSIGASSEM PFAGMISRAF QMGLADGPTE VHKVTVAKQL LRDYAPCEQM FPAYHRPTAA AAARRKFAAA LAQGAAR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCT GGGACTTCGA AACCGACGCC GATTTTCAGG CCGAACTCGA CTGGATCGAC CAGTTCGTGC GCGAGGAGGT CGAGCCGCTC GAACACGTGC TGGGCAGCCC GTGGAATATC CACGATCCGG CCTTCAAGGC GCTCGTCAAG CCGTTGCAGC AGCGGGTCAA GGAGCGCCGG CTGTGGGCCT GTCACCTCGG CCCCGAACTG GGCGGCCCCG GCTATGGACA GGTGAAGCTC GCGCTCATGA ACGAGATTCT GGGCCGCGCG CTGTTCGCGC CGATCGTGTT CGGCTGCCAG GCGCCGGACT CAGGCAACGC GGAAATCCTC GCGCATTACG GCACGGAGGC GCAGAAGGAG CGCTTCCTGA AACCATTGCT CGCCGGCGAC ATCGTGTCCT GCTTCGCGAT GACCGAGCCG CAGGGCGGCG CCGACCCCAA GGTGTTCACG ACCCGCGCGG TGCGCGACGG CGATGCGTGG GTGATCAACG GACGCAAGTG GTTCGCGTCG AATGCGCGCT ACGCGGACTT CTTCATCGTG ATGGCGATCA CCGATCCGGA AGTGTCGGCG TACAAGGGCA TGTCGATGTT CATCGTGCCG GCGAACACGC CCGGCCTGAG CATCATCCGC AATGTCGGCA TGGCCGACGA GCCGGAGGCG ACACACGCGT ACCTGGCGTT CGATGACGTG CGCGTGGCGG ACGAACAGAT GCTGGGCGCG CCGGGCGACG CGTTTGTCGT CGCGCAGGTG CGGCTTGGCG GCGGCCGGGT GCATCATGCG ATGCGCACGA TCGGACTCGC GCAGAAGGCG CTCGATGCGC TATGCGAGCG CGCGCTGTCG CGCCATACAC AGGGCGGCGC GCTCGCCGAC AAGCAGATGG TGCAGGAGAA GATCGCCGAC TCCTGGCTCG AACTCGAACA GTTTCGTCTG CTCGTGATGC GGACCGCGTG GCGCATCGAC CGTTACCAGG ACTATCAGAA GGTGCGCAAG GACATTGCCG CCGTGAAGGC CGCGATGCCC AGGGTATTGC ACGACATTGC CGCGCGCGCG CTGCATGTGC ATGGGTCGAT CGGAGCCTCG TCGGAGATGC CGTTCGCGGG AATGATCTCG CGTGCGTTTC AGATGGGGCT GGCCGATGGG CCGACCGAGG TTCACAAGGT GACGGTGGCG AAGCAACTGC TGCGCGACTA CGCGCCATGC GAGCAGATGT TTCCCGCCTA CCATCGCCCG ACGGCCGCCG CCGCCGCACG CCGGAAATTC GCGGCGGCGC TCGCTCAGGG AGCGGCACGA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT