SSGCID: Target Page

BuxeA.00027.b

Putative acyl-CoA dehydrogenase

CENTER ID:	BuxeA.00027.b
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00027.b.B1.GE39434	Full length( BuxeA.00027.b )		1	385

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.8598
RefSeq:	YP_556064.1
UniProt:	Q13GS5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTFMTEERRL IQASARQFAR EVVLPLANRL DPLKEDIPMS LRERMAGLGY FGILIPEEYG GLGLGVSEYC LITEELSRAW MSVGSIIRNL GLMVVQGMTD GQRQRWLPRM ACGEFLGALA MSEPNAGSDV AALTTRAIPD GDGWRITGSK CWCTFADGAD YIVVIARTAR VTDVARRHQG LSAFLIEKPR GALPAGVIGS PMPKIGYYGW KTWELSFDDV YVGPETLIGE PGRAFYYVAH GLETARAHTA ARSIGLAQGA LEDALDYATQ RVQFGHPIGD FQALRFRLAD MATRVETARQ LLRSVCEQID SGVRCEKEAA MAKYYAAEIS ETVTSDALQI HGGAGYTTHF AVERYWRDAR LTKIFEGTSE IQLRIISDHL LGKVA

NT Sequence

ATGACGTTCA TGACCGAGGA GCGCCGGCTG ATCCAGGCGT CGGCCCGGCA GTTCGCGCGC GAGGTGGTGT TGCCGCTCGC CAACCGGCTC GATCCGCTGA AGGAGGATAT CCCGATGTCC TTGCGCGAGC GGATGGCCGG ACTCGGCTAC TTCGGCATTC TGATCCCGGA GGAATACGGC GGGCTCGGGC TCGGCGTGTC CGAGTACTGC CTGATCACGG AGGAGCTATC GCGGGCCTGG ATGAGCGTCG GCAGCATCAT CCGCAACCTC GGGCTGATGG TCGTGCAGGG GATGACCGAC GGGCAGCGGC AGCGCTGGCT GCCGCGCATG GCATGCGGCG AATTCCTCGG TGCGCTGGCG ATGTCGGAGC CCAACGCCGG CTCAGACGTG GCCGCGCTCA CCACACGCGC GATACCGGAC GGCGACGGCT GGCGCATCAC GGGCTCGAAA TGCTGGTGCA CATTTGCCGA TGGCGCCGAC TACATCGTCG TGATCGCGCG CACCGCGCGG GTCACCGACG TGGCGAGGCG CCATCAGGGG CTGTCCGCGT TCCTGATCGA GAAGCCCCGT GGCGCATTGC CGGCGGGCGT GATCGGCTCG CCGATGCCGA AGATCGGCTA CTACGGCTGG AAGACGTGGG AGTTGTCGTT CGACGACGTC TACGTCGGCC CTGAGACGCT GATCGGCGAA CCGGGCCGCG CGTTCTACTA CGTTGCCCAC GGCCTCGAGA CGGCACGCGC GCATACCGCC GCGCGCTCGA TCGGATTGGC TCAGGGCGCT CTTGAGGATG CACTCGACTA CGCGACCCAG CGCGTGCAGT TCGGCCACCC AATCGGTGAC TTTCAGGCGC TGCGTTTCCG TCTCGCCGAC ATGGCGACGC GTGTCGAGAC CGCCCGGCAA CTGCTGCGCT CGGTGTGCGA GCAGATCGAC AGCGGCGTGC GCTGCGAGAA GGAAGCCGCG ATGGCCAAGT ACTACGCAGC GGAGATCAGC GAGACGGTCA CCAGCGATGC GTTACAGATC CACGGCGGCG CCGGCTACAC GACCCACTTC GCGGTCGAGC GCTACTGGCG CGACGCACGC CTGACCAAGA TCTTCGAGGG CACCTCTGAG ATCCAGCTAC GCATCATTTC CGATCACTTG CTGGGCAAGG TCGCA

Details for BuxeA.00027.b.B1.GE39434

HARVESTED ON:	8/5/2015
SEQUENCED ON:	8/13/2015
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMT FMTEERRLIQ ASARQFAREV VLPLANRLDP LKEDIPMSLR ERMAGLGYFG ILIPEEYGGL GLGVSEYCLI TEELSRAWMS VGSIIRNLGL MVVQGMTDGQ RQRWLPRMAC GEFLGALAMS EPNAGSDVAA LTTRAIPDGD GWRITGSKCW CTFADGADYI VVIARTARVT DVARRHQGLS AFLIEKPRGA LPAGVIGSPM PKIGYYGWKT WELSFDDVYV GPETLIGEPG RAFYYVAHGL ETARAHTAAR SIGLAQGALE DALDYATQRV QFGHPIGDFQ ALRFRLADMA TRVETARQLL RSVCEQIDSG VRCEKEAAMA KYYAAEISET VTSDALQIHG GAGYTTHFAV ERYWRDARLT KIFEGTSEIQ LRII

Validated NT Sequence

gaaatgatgc gtagctggat ctcagaggtg ccctcgaaga tcttggtcag gcgtgcgtcg cgccagtagc gctcgaccgc gaagtgggtc gtgtagccgg cgccgccgtg gatctgtaac gcatcgctgg tgaccgtctc gctgatctcc gctgcgtagt acttggccat cgcggcttcc ttctcgcagc gcacgccgct gtcgatctgc tcgcacaccg agcgcagcag ttgccgggcg gtctcgacac gcgtcgccat gtcggcgaga cggaaacgca gcgcctgaaa gtcaccgatt gggtggccga actgcacgcg ctgggtcgcg tagtcgagtg catcctcaag agcgccctga gccaatccga tcgagcgcgc ggcggtatgc gcgcgtgccg tctcgaggcc gtgggcaacg tagtagaacg cgcggcccgg ttcgccgatc agcgtctcag ggccgacgta gacgtcgtcg aacgacaact cccacgtctt ccagccgtag tagccgatct tcggcatcgg cgagccgatc acgcccgccg gcaatgcgcc acggggcttc tcgatcagga acgcggacag cccctgatgg cgcctcgcca cgtcggtgac ccgcgcggtg cgcgcgatca cgacgatgta gtcggcgcca tcggcaaatg tgcaccagca tttcgagccc gtgatgcgcc agccgtcgcc gtccggtatc gcgcgtgtgg tgagcgcggc cacgtctgag ccggcgttgg gctccgacat cgccagcgca ccgaggaatt cgccgcatgc catgcgcggc agccagcgct gccgctgccc gtcggtcatc ccctgcacga ccatcagccc gaggttgcgg atgatgctgc cgacgctcat ccaggcccgc gatagctcct ccgtgatcag gcagtactcg gacacgccga gcccgagccc gccgtattcc tccgggatca gaatgccgaa gtagccgagt ccggccatcc gctcgcgcaa ggacatcggg atatcctcct tcagcggatc gagccggttg gcgagcggca acaccacctc gcgcgcgaac tgccgggccg acgcctggat cagccggcgc tcctcggtca tgaacgtcat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgt tcatgaccga ggagcgccgg ctgatccagg cgtcggcccg gcagttcgcg cgcgaggtgg tgttgccgct cgccaaccgg ctcgatccgc tgaaggagga tatcccgatg tccttgcgcg agcggatggc cggactcggc tacttcggca ttctgatccc ggaggaatac ggcgggctcg ggctcggcgt gtccgagtac tgcctgatca cggaggagct atcgcgggcc tggatgagcg tcggcagcat catccgcaac ctcgggctga tggtcgtgca ggggatgacc gacgggcagc ggcagcgctg gctgccgcgc atggcatgcg gcgaattcct cggtgcgctg gcgatgtcgg agcccaacgc cggctcagac gtggccgcgc tcaccacacg cgcgataccg gacggcgacg gctggcgcat cacgggctcg aaatgctggt gcacatttgc cgatggcgcc gactacatcg tcgtgatcgc gcgcaccgcg cgggtcaccg acgtggcgag gcgccatcag gggctgtccg cgttcctgat cgagaagccc cgtggcgcat tgccggcggg cgtgatcggc tcgccgatgc cgaagatcgg ctactacggc tggaagacgt gggagttgtc gttcgacgac gtctacgtcg gccctgagac gctgatcggc gaaccgggcc gcgcgttcta ctacgttgcc cacggcctcg agacggcacg cgcgcatacc gccgcgcgct cgatcggatt ggctcagggc gctcttgagg atgcactcga ctacgcgacc cagcgcgtgc agttcggcca cccaatcggt gactttcagg cgctgcgttt ccgtctcgcc gacatggcga cgcgtgtcga gaccgcccgg caactgctgc gctcggtgtg cgagcagatc gacagcggcg tgcgctgcga gaaggaagcc gcgatggcca agtactacgc agcggagatc agcgagacgg tcaccagcga tgcgttacag atccacggcg gcgccggcta cacgacccac ttcgcggtcg agcgctactg gcgcgacgca cgcctgacca agatcttcga gggcacctct gagatccagc tacgcatcat ttccgatcac ttgctgggca aggtcgcatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat