SSGCID: Target Page

BuxeA.00027.a

Putative acyl-CoA dehydrogenase

CENTER ID:	BuxeA.00027.a
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00027.a.B1.GE39433	Full length( BuxeA.00027.a )		1	390

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.8616
RefSeq:	YP_556081.1
UniProt:	Q13GQ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDFRFDESQR MMIDTARQIG QRFGLEYWRK QDAARSFPAE YWRTVCEAGL CGVALPEEYG GSGLGMMEMS LIVENLAATG GGSTVGQLFM INPIFGGVSI ARFGPEAMRK ELLPGIISGE INCCMALTEP DAGTNTLEIR TFASADGDGW RLNGRKIWIT GVQSANKMLI VARTKKVADS TSRTDGLSMF MIDVQREGLS FTPIDKLGTC TLDSSSVFFD DVAIEPTELI GTLHQGWREL LDVLNTERIV TTAGLVGTGE LAIQLAVDYA NDRKVFGNTP ISAYQGLQFP LAQCHVEVQA ARLLNYKAAT QFDMGLPYGS EANAAKLLAA QAVSRATERA MQTMGGMGYS KEFHVERLWR DCRLFRFAPV SEEMILNYVA NHDLKMPRSY

NT Sequence

ATGGACTTTC GATTTGACGA GTCACAGCGA ATGATGATCG ACACGGCGCG CCAGATTGGC CAACGCTTCG GTCTGGAGTA CTGGCGCAAG CAGGACGCGG CCAGGTCGTT TCCGGCCGAA TACTGGCGCA CGGTCTGCGA GGCCGGTTTG TGCGGCGTGG CGCTGCCCGA GGAATACGGC GGAAGCGGGC TCGGCATGAT GGAGATGTCA CTCATCGTCG AGAATCTCGC GGCGACCGGC GGCGGCTCCA CCGTAGGTCA GCTCTTCATG ATCAATCCGA TCTTCGGCGG CGTGTCGATT GCCCGCTTCG GCCCCGAGGC AATGCGAAAA GAACTGCTGC CGGGCATCAT CAGCGGCGAG ATCAACTGTT GCATGGCGCT CACCGAGCCG GATGCGGGCA CCAACACGCT GGAGATCCGC ACTTTCGCCA GTGCCGACGG CGACGGCTGG CGCCTGAACG GCCGCAAGAT CTGGATCACG GGCGTGCAAT CGGCGAACAA GATGCTGATC GTTGCGCGCA CGAAAAAGGT CGCGGACTCG ACCTCGCGAA CCGATGGCCT CTCGATGTTC ATGATCGATG TGCAACGCGA AGGCCTGTCT TTCACACCCA TCGATAAGCT CGGCACCTGC ACGCTCGATT CCAGCAGTGT GTTTTTCGAC GACGTCGCGA TTGAGCCCAC CGAACTGATC GGCACGCTGC ATCAAGGTTG GCGTGAGTTG CTGGACGTGC TGAACACGGA GCGCATCGTG ACGACCGCGG GTCTCGTCGG CACGGGCGAG CTGGCGATCC AGCTCGCCGT CGACTATGCA AACGACCGCA AGGTATTCGG CAACACACCC ATCAGCGCCT ACCAGGGTTT GCAGTTTCCG CTCGCACAGT GTCACGTGGA AGTGCAAGCC GCGCGCCTGC TCAACTATAA GGCCGCGACG CAGTTCGACA TGGGTCTTCC GTATGGCAGC GAGGCCAATG CCGCCAAACT CCTGGCCGCG CAGGCCGTGA GCCGCGCGAC GGAGCGCGCG ATGCAAACGA TGGGTGGCAT GGGTTATTCG AAGGAGTTTC ACGTCGAGCG CCTATGGCGC GATTGCCGCC TGTTCCGGTT CGCACCGGTT TCGGAAGAAA TGATCCTGAA CTACGTCGCG AATCATGATC TGAAGATGCC GCGCTCGTAC

Details for BuxeA.00027.a.B1.GE39433

HARVESTED ON:	data unavailable
SEQUENCED ON:	8/13/2015
EXPECTED MW:	44kDa
OBSERVED MW:	0kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	None
TOTAL EXPRESSION LEVEL:	Data Unavailable
SOLUBLE EXPRESSION LEVEL	Data Unavailable
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMD FRFDESQRMM IDTARQIGQR FGLEYWRKQD AARSFPAEYW RTVCEAGLCG VALPEEYGGS GLGMMEMSLI VENLAATGGG STVGQLFMIN PIFGGVSIAR FGPEAMRKEL LPGIISGEIN CCMALTEPDA GTNTLEIRTF ASADGDGWRL NGRKIWITGV QSANKMLIVA RTKKVADSTS RTDGLSMFMI DVQREGLSFT PIDKLGTCTL DSSSVFFDDV AIEPTELIGT LHQGWRELLD VLNTERIVTT AGLVGTGELA IQLAVDYAND RKVFGNTPIS AYQGLQFPLA QCHVEVQAAR LLNYKAATQF DMGLPYGSEA NAAKLLAAQA VSRATERAMQ TMGGMGYSKE FHVERLWRDC RLFRFAPVSE EMILNYVANH

Validated NT Sequence

catgattcgc gacgtagttc aggatcattt cttccgaaac cggtgcgaac cggaacaggc ggcaatcgcg ccataggcgc tcgacgtgaa actccttcga ataacccatg ccacccatcg tttgcatcgc gcgctccgtc gcgcggctca cggcctgcgc ggccaggagt ttggcggcat tggcctcgct gccatacgga agacccatgt cgaactgcgt cgcggcctta tagttgagca ggcgcgcggc ttgcacttcc acgtgacact gtgcgagcgg aaactgcaaa ccctggtagg cgctgatggg tgtgttgccg aataccttgc ggtcgtttgc atagtcgacg gcgagctgga tcgccagctc gcccgtgccg acgagacccg cggtcgtcac gatgcgctcc gtgttcagca cgtccagcaa ctcacgccaa ccttgatgca gcgtgccgat cagttcggtg ggctcaatcg cgacgtcgtc gaaaaacaca ctgctggaat cgagcgtgca ggtgccgagc ttatcgatgg gtgtgaaaga caggccttcg cgttgcacat cgatcatgaa catcgagagg ccatcggttc gcgaggtcga gtccgcgacc tttttcgtgc gcgcaacgat cagcatcttg ttcgccgatt gcacgcccgt gatccagatc ttgcggccgt tcaggcgcca gccgtcgccg tcggcactgg cgaaagtgcg gatctccagc gtgttggtgc ccgcatccgg ctcggtgagc gccatgcaac agttgatctc gccgctgatg atgcccggca gcagttcttt tcgcattgcc tcggggccga agcgggcaat cgacacgccg ccgaagatcg gattgatcat gaagagctga cctacggtgg agccgccgcc ggtcgccgcg agattctcga cgatgagtga catctccatc atgccgagcc cgcttccgcc gtattcctcg ggcagcgcca cgccgcacaa accggcctcg cagaccgtgc gccagtattc ggccggaaac gacctggccg cgtcctgctt gcgccagtac tccagaccga agcgttggcc aatctggcgc gccgtgtcga tcatcattcg ctgtgactcg tcaaatcgaa agtccatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggact ttcgatttga cgagtcacag cgaatgatga tcgacacggc gcgccagatt ggccaacgct tcggtctgga gtactggcgc aagcaggacg cggccaggtc gtttccggcc gaatactggc gcacggtctg cgaggccggt ttgtgcggcg tggcgctgcc cgaggaatac ggcggaagcg ggctcggcat gatggagatg tcactcatcg tcgagaatct cgcggcgacc ggcggcggct ccaccgtagg tcagctcttc atgatcaatc cgatcttcgg cggcgtgtcg attgcccgct tcggccccga ggcaatgcga aaagaactgc tgccgggcat catcagcggc gagatcaact gttgcatggc gctcaccgag ccggatgcgg gcaccaacac gctggagatc cgcactttcg ccagtgccga cggcgacggc tggcgcctga acggccgcaa gatctggatc acgggcgtgc aatcggcgaa caagatgctg atcgttgcgc gcacgaaaaa ggtcgcggac tcgacctcgc gaaccgatgg cctctcgatg ttcatgatcg atgtgcaacg cgaaggcctg tctttcacac ccatcgataa gctcggcacc tgcacgctcg attccagcag tgtgtttttc gacgacgtcg cgattgagcc caccgaactg atcggcacgc tgcatcaagg ttggcgtgag ttgctggacg tgctgaacac ggagcgcatc gtgacgaccg cgggtctcgt cggcacgggc gagctggcga tccagctcgc cgtcgactat gcaaacgacc gcaaggtatt cggcaacaca cccatcagcg cctaccaggg tttgcagttt ccgctcgcac agtgtcacgt ggaagtgcaa gccgcgcgcc tgctcaacta taaggccgcg acgcagttcg acatgggtct tccgtatggc agcgaggcca atgccgccaa actcctggcc gcgcaggccg tgagccgcgc gacggagcgc gcgatgcaaa cgatgggtgg catgggttat tcgaaggagt ttcacgtcga gcgcctatgg cgcgattgcc gcctgttccg gttcgcaccg gtttcggaag aaatgatcct gaactacgtc gcgaatcatg atctgaagat gccgcgctcg tactgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat