SSGCID: Target Page

BuxeA.00024.j

Taurine dioxygenase

CENTER ID:	BuxeA.00024.j
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00024.j.B2.GE39350	Mature protein( BuxeA.00024.j )		16	302

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.2364
RefSeq:	YP_558852.1
UniProt:	Q13YN9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTTAFASTAA SQAGLAQPAQ HIEIRAFDGP VGAEVLGLDL GRPLSQHDFE RIHRAHLDHH VLVFRDQRIT PDQQIAFSRR FGPLQIHVLH QFQLPGHPEV LVVSNIVENG QPIGLGDAGH FWHSDLSYKE TPSLGSLLHA QELPAQGGDT LFANMHLAWD TFPAHLRAAV EGRSAEHTYL AKYAELQKRS PWRPDLSAEQ IAQVKPVVHP IVRTHPETGR KALFVSEHFT TRVIGLPEDE SRSLLEEIFA HSVRPEHLYR HEWSEHDMVF WDNRSLMHLA AGTPDHLRRK LYRTTIEGDV PF

NT Sequence

GTGACGACTG CCTTTGCATC CACCGCCGCC AGCCAGGCTG GCCTTGCCCA ACCGGCCCAG CACATCGAGA TTCGCGCGTT CGACGGCCCC GTCGGCGCGG AAGTGCTCGG CCTCGATCTC GGCCGGCCGC TCAGCCAGCA CGATTTCGAG CGCATTCACC GTGCGCATCT GGACCATCAC GTGCTGGTGT TCCGCGATCA ACGCATCACG CCGGATCAGC AGATCGCTTT CAGCCGCCGC TTCGGTCCGC TGCAGATTCA CGTGCTGCAT CAGTTCCAAC TGCCGGGCCA TCCGGAGGTG CTGGTCGTCT CGAACATCGT CGAGAACGGC CAGCCGATCG GTCTGGGCGA CGCCGGCCAT TTCTGGCACT CCGATCTGTC CTACAAGGAG ACGCCGAGCC TCGGTTCACT GCTGCATGCG CAGGAGTTGC CTGCGCAAGG CGGCGACACG CTGTTCGCCA ACATGCATCT GGCGTGGGAC ACGTTCCCGG CGCATTTGCG CGCCGCAGTG GAAGGGCGTT CGGCGGAACA CACGTATCTC GCGAAATACG CTGAGCTGCA AAAGCGCAGC CCGTGGCGCC CGGATCTCTC CGCCGAGCAG ATCGCCCAGG TCAAGCCGGT GGTGCACCCG ATCGTCCGCA CGCATCCGGA GACCGGCCGC AAGGCGCTCT TTGTCAGCGA GCATTTCACG ACTCGCGTGA TCGGCTTGCC GGAGGACGAG AGCCGGTCTC TGCTCGAGGA AATTTTCGCG CATAGCGTGC GCCCCGAGCA TCTGTACCGG CATGAGTGGT CCGAACACGA CATGGTGTTC TGGGACAACC GCTCGTTGAT GCACCTCGCG GCCGGCACGC CGGATCATCT GCGCCGCAAA CTGTACCGCA CGACAATTGA AGGCGACGTG CCGTTC

Details for BuxeA.00024.j.B2.GE39350

HARVESTED ON:	7/10/2015
SEQUENCED ON:	7/7/2015
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	50

Validated AA Sequence

MHLAWDTFPA HLRAAVEGRS AEHTYLAKYA ELQKRSPWRP DLSAEQIAQV KPVVHPIVRT HPETGRKALF VSEHFTTRVI GLPEDESRSL LEEIFAHSVR PEHLYRHEWS EHDMVFWDNR SLMHLAAGTP DHLRRKLYRT XIEGDVPF

Validated NT Sequence

atcttactca cttagaacgg cacgtcgcct tcaattgncg tgcggtacag tttgcggcgc agatgatccg gcgtgccggc cgcgaggtgc atcaacgagc ggttgtccca gaacaccatg tcgtgttcgg accactcatg ccggtacaga tgctcggggc gcacgctatg cgcgaaaatt tcctcgagca gagaccggct ctcgtcctcc ggcaagccga tcacgcgagt cgtgaaatgc tcgctgacaa agagcgcctt gcggccggtc tccggatgcg tgcggacgat cgggtgcacc accggcttga cctgggcgat ctgctcggcg gagagatccg ggcgccacgg gctgcgcttt tgcagctcag cgtatttcgc gagatacgtg tgttccgccg aacgcccttc cactgcggcg cgcaaatgcg ccgggaacgt gtcccacgcc agatgcatgt tggcgaacag cgtgtcnnng cct

Expected Protein Sequence

MAHHHHHHAQ PAQHIEIRAF DGPVGAEVLG LDLGRPLSQH DFERIHRAHL DHHVLVFRDQ RITPDQQIAF SRRFGPLQIH VLHQFQLPGH PEVLVVSNIV ENGQPIGLGD AGHFWHSDLS YKETPSLGSL LHAQELPAQG GDTLFANMHL AWDTFPAHLR AAVEGRSAEH TYLAKYAELQ KRSPWRPDLS AEQIAQVKPV VHPIVRTHPE TGRKALFVSE HFTTRVIGLP EDESRSLLEE IFAHSVRPEH LYRHEWSEHD MVFWDNRSLM HLAAGTPDHL RRKLYRTTIE GDVPF

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcccaac cggcccagca catcgagatt cgcgcgttcg acggccccgt cggcgcggaa gtgctcggcc tcgatctcgg ccggccgctc agccagcacg atttcgagcg cattcaccgt gcgcatctgg accatcacgt gctggtgttc cgcgatcaac gcatcacgcc ggatcagcag atcgctttca gccgccgctt cggtccgctg cagattcacg tgctgcatca gttccaactg ccgggccatc cggaggtgct ggtcgtctcg aacatcgtcg agaacggcca gccgatcggt ctgggcgacg ccggccattt ctggcactcc gatctgtcct acaaggagac gccgagcctc ggttcactgc tgcatgcgca ggagttgcct gcgcaaggcg gcgacacgct gttcgccaac atgcatctgg cgtgggacac gttcccggcg catttgcgcg ccgcagtgga agggcgttcg gcggaacaca cgtatctcgc gaaatacgct gagctgcaaa agcgcagccc gtggcgcccg gatctctccg ccgagcagat cgcccaggtc aagccggtgg tgcacccgat cgtccgcacg catccggaga ccggccgcaa ggcgctcttt gtcagcgagc atttcacgac tcgcgtgatc ggcttgccgg aggacgagag ccggtctctg ctcgaggaaa ttttcgcgca tagcgtgcgc cccgagcatc tgtaccggca tgagtggtcc gaacacgaca tggtgttctg ggacaaccgc tcgttgatgc acctcgcggc cggcacgccg gatcatctgc gccgcaaact gtaccgcacg acaattgaag gcgacgtgcc gttctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat