BuxeA.00024.h

Taurine dioxygenase

CENTER ID: BuxeA.00024.h
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00024.h.B1.GE39348 Full length( BuxeA.00024.h ) 1 305
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.4978
RefSeq: YP_552626.1
UniProt: Q13RL3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQATEIAPDT AVVHSNSDAW PFVVERYTPT IGAEIRGVDL RDPLDDETYA ALRRALLKFK VLFFRDQDIT PAQHVAVAKR FGELEIHPAF PHHPEHPELV ILGRNDTKRG RENLYHSDVS WREIPSMGSM LRCVQCPEVG GDTMWINMVA AYDNLPGDVK ARIAGLNAVH DFLPLFGIAV PKDQHATMRA KFPPVEHPVV RIHPETGEKI LYVNEAFTTH LSNYGQLTAT EYRVGFDYKL AELDLLQYLF RQAQAPEYQV RLKWRPNTIA FWDNRSCQHY AVQDYFPAPR HMMRATVIGD RPLAA
NT Sequence
ATGCAAGCAA CTGAGATCGC ACCCGATACG GCAGTAGTGC ACTCGAATTC CGATGCCTGG CCGTTCGTCG TCGAACGCTA TACACCGACC ATCGGTGCGG AGATTCGCGG CGTCGATCTG CGCGATCCGC TCGACGACGA GACCTACGCT GCACTGCGCC GCGCACTGTT GAAGTTCAAG GTGCTGTTCT TCCGCGACCA GGACATCACT CCCGCGCAGC ATGTCGCGGT GGCGAAGCGA TTCGGAGAAC TGGAGATCCA TCCTGCGTTT CCCCACCATC CCGAACATCC GGAACTGGTG ATACTCGGCC GCAACGATAC CAAACGCGGC CGCGAAAACC TGTACCACTC CGACGTATCG TGGCGCGAGA TTCCATCAAT GGGCTCCATG TTGCGATGCG TGCAATGCCC AGAGGTCGGC GGCGACACCA TGTGGATCAA CATGGTGGCG GCCTACGACA ACCTGCCCGG CGACGTGAAG GCGAGAATCG CCGGCCTCAA CGCGGTTCAC GACTTCCTCC CGCTATTCGG CATTGCCGTG CCCAAGGACC AGCACGCGAC CATGCGCGCG AAGTTTCCAC CCGTCGAGCA TCCGGTTGTG CGGATTCATC CCGAAACCGG CGAGAAGATC CTCTACGTCA ACGAGGCGTT TACCACGCAT CTGTCGAACT ACGGCCAGTT GACTGCCACG GAATATCGCG TCGGTTTCGA CTACAAGCTC GCCGAACTGG ATCTGCTGCA GTACCTGTTC CGCCAGGCCC AGGCGCCGGA ATATCAGGTG CGCCTCAAGT GGCGCCCGAA CACGATCGCC TTCTGGGACA ACCGCTCGTG TCAGCACTAC GCGGTCCAGG ACTACTTCCC GGCACCGCGC CACATGATGC GTGCGACGGT GATCGGCGAT CGTCCGCTAG CCGCA
Details for BuxeA.00024.h.B1.GE39348
HARVESTED ON: 7/10/2015
SEQUENCED ON: 7/6/2015
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMQ ATEIAPDTAV VHSNSDAWPF VVERYTPTIG AEIRGVDLRD PLDDETYAAL RRALLKFKVL FFRDQDITPA QHVAVAKRFG ELEIHPAFPH HPEHPELVIL GRNDTKRGRE NLYHSDVSWR EIPSMGSMLR CVQCPEVGGD TMWINMVAAY DNLPGDVKAR IAGLNAVHDF LPLFGIAVPK DQHATMRAKF PPVEHPVVRI HPETGEKILY VNEAFTTHLS NYGQLTATEY RVGFDYKLAE LDLLQYLFRQ AQAPEYQVRL KWRPNTIAFW DNRSCQHYAV QDYFPAPRHM MRATVIGDRP LAA
Validated NT Sequence
tcacttatgc ggctagcgga cgatcgccga tcaccgtcgc acgcatcatg tggcgcggtg ccgggaagta gtcctggacc gcgtagtgct gacacgagcg gttgtcccag aaggcgatcg tgttcgggcg ccacttgagg cgcacctgat attccggcgc ctgggcctgg cggaacaggt actgcagcag atccagttcg gcgagcttgt agtcgaaacc gacgcgatat tccgtggcag tcaactggcc gtagttcgac agatgcgtgg taaacgcctc gttgacgtag aggatcttct cgccggtttc gggatgaatc cgcacaaccg gatgctcgac gggtggaaac ttcgcgcgca tggtcgcgtg ctggtccttg ggcacggcaa tgccgaatag cgggaggaag tcgtgaaccg cgttgaggcc ggcgattctc gccttcacgt cgccgggcag gttgtcgtag gccgccacca tgttgatcca catggtgtcg ccgccgacct ctgggcattg cacgcatcgc aacatggagc ccattgatgg aatctcgcgc cacgatacgt cggagtggta caggttttcg cggccgcgtt tggtatcgtt gcggccgagt atcaccagtt ccggatgttc gggatggtgg ggaaacgcag gatggatctc cagttctccg aatcgcttcg ccaccgcgac atgctgcgcg ggagtgatgt cctggtcgcg gaagaacagc accttgaact tcaacagtgc gcggcgcagt gcagcgtagg tctcgtcgtc gagcggatcg cgcagatcga cgccgcgaat ctccgcaccg atggtcggtg tatagcgttc gacgacgaac ggccaggcat cggaattcga gtgcactact gccgtatcgg gtgcgatctc agttgcttgc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMQ ATEIAPDTAV VHSNSDAWPF VVERYTPTIG AEIRGVDLRD PLDDETYAAL RRALLKFKVL FFRDQDITPA QHVAVAKRFG ELEIHPAFPH HPEHPELVIL GRNDTKRGRE NLYHSDVSWR EIPSMGSMLR CVQCPEVGGD TMWINMVAAY DNLPGDVKAR IAGLNAVHDF LPLFGIAVPK DQHATMRAKF PPVEHPVVRI HPETGEKILY VNEAFTTHLS NYGQLTATEY RVGFDYKLAE LDLLQYLFRQ AQAPEYQVRL KWRPNTIAFW DNRSCQHYAV QDYFPAPRHM MRATVIGDRP LAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaag caactgagat cgcacccgat acggcagtag tgcactcgaa ttccgatgcc tggccgttcg tcgtcgaacg ctatacaccg accatcggtg cggagattcg cggcgtcgat ctgcgcgatc cgctcgacga cgagacctac gctgcactgc gccgcgcact gttgaagttc aaggtgctgt tcttccgcga ccaggacatc actcccgcgc agcatgtcgc ggtggcgaag cgattcggag aactggagat ccatcctgcg tttccccacc atcccgaaca tccggaactg gtgatactcg gccgcaacga taccaaacgc ggccgcgaaa acctgtacca ctccgacgta tcgtggcgcg agattccatc aatgggctcc atgttgcgat gcgtgcaatg cccagaggtc ggcggcgaca ccatgtggat caacatggtg gcggcctacg acaacctgcc cggcgacgtg aaggcgagaa tcgccggcct caacgcggtt cacgacttcc tcccgctatt cggcattgcc gtgcccaagg accagcacgc gaccatgcgc gcgaagtttc cacccgtcga gcatccggtt gtgcggattc atcccgaaac cggcgagaag atcctctacg tcaacgaggc gtttaccacg catctgtcga actacggcca gttgactgcc acggaatatc gcgtcggttt cgactacaag ctcgccgaac tggatctgct gcagtacctg ttccgccagg cccaggcgcc ggaatatcag gtgcgcctca agtggcgccc gaacacgatc gccttctggg acaaccgctc gtgtcagcac tacgcggtcc aggactactt cccggcaccg cgccacatga tgcgtgcgac ggtgatcggc gatcgtccgc tagccgcatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat