BuxeA.00024.f

Putative alpha KG dependent 2,4-D dioxygenase

CENTER ID:	BuxeA.00024.f
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	native diffraction-data
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00024.f.B1.GE39346	Full length( BuxeA.00024.f )		1	301

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.00024.f.B1.PS02505	Full length( BuxeA.00024.f )		1	301

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_155768
PATRIC ID:	fig\|266265.5.peg.5572
RefSeq:	YP_553181.1
UniProt:	Q13Q08

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAFTVEVVHP LLAARIHGLD LHQPVALEDV EAIEQTSGRY PVLIFPKQFI NDDELLAFSA NFGPVHTAMS YQTREKEHRL QPKITDISNL GKDNQTFKRG DHRRMNNFVS KRWHTDASYE AIPARYSFLL AYTVAQRGGE TQFADMRAAY DEMPEDLRPV VEDLSCEYNI LWARMLSGYT EFPDEERAKL PPAQHKLVRQ HPVSGRKTLY LSGHATHVVD WPIPEGRDLL RELMEFATQP QFVYTHKWHE RDLVMWDNRA LMHRGRRHIP DTDPREMHRA TVMDDPAWTR ADSSVHSEAG T

NT Sequence

ATGGCGTTTA CCGTTGAGGT CGTGCACCCG TTATTGGCCG CCCGCATTCA CGGCCTCGAT CTGCATCAAC CCGTCGCGCT GGAAGATGTC GAGGCGATCG AGCAGACAAG CGGCCGTTAT CCGGTTCTGA TCTTTCCGAA GCAGTTCATC AACGACGACG AACTGCTCGC GTTCAGCGCG AACTTCGGGC CGGTCCACAC CGCCATGTCG TATCAGACGC GTGAGAAAGA GCACCGGTTG CAGCCGAAGA TCACCGACAT CTCGAACCTC GGCAAGGACA ATCAGACGTT CAAGCGCGGT GATCATCGCC GGATGAACAA CTTCGTCAGC AAGCGGTGGC ATACGGACGC TTCATATGAA GCAATTCCGG CGCGCTACTC GTTCCTGCTG GCGTATACCG TGGCGCAGCG GGGCGGCGAG ACACAGTTCG CCGATATGCG GGCGGCGTAC GACGAGATGC CTGAAGATCT TCGCCCGGTA GTCGAAGATC TGAGCTGCGA GTACAACATC TTGTGGGCGC GCATGCTGAG CGGCTACACC GAGTTTCCGG ACGAGGAGCG GGCGAAGCTG CCGCCCGCGC AGCACAAGCT CGTCAGGCAG CATCCCGTGT CGGGACGCAA GACGCTCTAT CTGTCGGGGC ACGCGACGCA CGTAGTGGAC TGGCCGATTC CCGAAGGCCG CGACCTGTTG CGCGAATTGA TGGAATTCGC GACGCAGCCC CAGTTCGTCT ATACCCATAA ATGGCACGAA CGCGATCTGG TGATGTGGGA CAACCGCGCG TTGATGCACC GCGGGCGCCG CCATATTCCC GATACTGACC CGCGCGAGAT GCATCGCGCG ACGGTAATGG ACGATCCTGC GTGGACGCGA GCGGACAGCA GCGTGCACAG CGAAGCGGGA ACG

Details for BuxeA.00024.f.B1.GE39346

HARVESTED ON:	7/10/2015
SEQUENCED ON:	7/6/2015
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA FTVEVVHPLL AARIHGLDLH QPVALEDVEA IEQTSGRYPV LIFPKQFIND DELLAFSANF GPVHTAMSYQ TREKEHRLQP KITDISNLGK DNQTFKRGDH RRMNNFVSKR WHTDASYEAI PARYSFLLAY TVAQRGGETQ FADMRAAYDE MPEDLRPVVE DLSCEYNILW ARMLSGYTEF PDEERAKLPP AQHKLVRQHP VSGRKTLYLS GHATHVVDWP IPEGRDLLRE LMEFATQPQF VYTHKWHERD LVMWDNRALM HRGRRHIPDT DPREMHRATV MDDPAWTRAD SSVHSEAGT

Validated NT Sequence

tcacttacgt tcccgcttcg ctgtgcacgc tgctgtccgc tcgcgtccac gcaggatcgt ccattaccgt cgcgcgatgc atctcgcgcg ggtcagtatc gggaatatgg cggcgcccgc ggtgcatcaa cgcgcggttg tcccacatca ccagatcgcg ttcgtgccat ttatgggtat agacgaactg gggctgcgtc gcgaattcca tcaattcgcg caacaggtcg cggccttcgg gaatcggcca gtccactacg tgcgtcgcgt gccccgacag atagagcgtc ttgcgtcccg acacgggatg ctgcctgacg agcttgtgct gcgcgggcgg cagcttcgcc cgctcctcgt ccggaaactc ggtgtagccg ctcagcatgc gcgcccacaa gatgttgtac tcgcagctca gatcttcgac taccgggcga agatcttcag gcatctcgtc gtacgccgcc cgcatatcgg cgaactgtgt ctcgccgccc cgctgcgcca cggtatacgc cagcaggaac gagtagcgcg ccggaattgc ttcatatgaa gcgtccgtat gccaccgctt gctgacgaag ttgttcatcc ggcgatgatc accgcgcttg aacgtctgat tgtccttgcc gaggttcgag atgtcggtga tcttcggctg caaccggtgc tctttctcac gcgtctgata cgacatggcg gtgtggaccg gcccgaagtt cgcgctgaac gcgagcagtt cgtcgtcgtt gatgaactgc ttcggaaaga tcagaaccgg ataacggccg cttgtctgct cgatcgcctc gacatcttcc agcgcgacgg gttgatgcag atcgaggccg tgaatgcggg cggccaataa cgggtgcacg acctcaacgg taaacgccat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgt ttaccgttga ggtcgtgcac ccgttattgg ccgcccgcat tcacggcctc gatctgcatc aacccgtcgc gctggaagat gtcgaggcga tcgagcagac aagcggccgt tatccggttc tgatctttcc gaagcagttc atcaacgacg acgaactgct cgcgttcagc gcgaacttcg ggccggtcca caccgccatg tcgtatcaga cgcgtgagaa agagcaccgg ttgcagccga agatcaccga catctcgaac ctcggcaagg acaatcagac gttcaagcgc ggtgatcatc gccggatgaa caacttcgtc agcaagcggt ggcatacgga cgcttcatat gaagcaattc cggcgcgcta ctcgttcctg ctggcgtata ccgtggcgca gcggggcggc gagacacagt tcgccgatat gcgggcggcg tacgacgaga tgcctgaaga tcttcgcccg gtagtcgaag atctgagctg cgagtacaac atcttgtggg cgcgcatgct gagcggctac accgagtttc cggacgagga gcgggcgaag ctgccgcccg cgcagcacaa gctcgtcagg cagcatcccg tgtcgggacg caagacgctc tatctgtcgg ggcacgcgac gcacgtagtg gactggccga ttcccgaagg ccgcgacctg ttgcgcgaat tgatggaatt cgcgacgcag ccccagttcg tctataccca taaatggcac gaacgcgatc tggtgatgtg ggacaaccgc gcgttgatgc accgcgggcg ccgccatatt cccgatactg acccgcgcga gatgcatcgc gcgacggtaa tggacgatcc tgcgtggacg cgagcggaca gcagcgtgca cagcgaagcg ggaacgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for BuxeA.00024.f.B1.PS02505

PURIFICATION DATe:	10/1/2015
CONCENTRATION:	35.99mg/ml
OBSERVED MW:	37kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC buffer
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCGT TTACCGTTGA GGTCGTGCAC CCGTTATTGG CCGCCCGCAT TCACGGCCTC GATCTGCATC AACCCGTCGC GCTGGAAGAT GTCGAGGCGA TCGAGCAGAC AAGCGGCCGT TATCCGGTTC TGATCTTTCC GAAGCAGTTC ATCAACGACG ACGAACTGCT CGCGTTCAGC GCGAACTTCG GGCCGGTCCA CACCGCCATG TCGTATCAGA CGCGTGAGAA AGAGCACCGG TTGCAGCCGA AGATCACCGA CATCTCGAAC CTCGGCAAGG ACAATCAGAC GTTCAAGCGC GGTGATCATC GCCGGATGAA CAACTTCGTC AGCAAGCGGT GGCATACGGA CGCTTCATAT GAAGCAATTC CGGCGCGCTA CTCGTTCCTG CTGGCGTATA CCGTGGCGCA GCGGGGCGGC GAGACACAGT TCGCCGATAT GCGGGCGGCG TACGACGAGA TGCCTGAAGA TCTTCGCCCG GTAGTCGAAG ATCTGAGCTG CGAGTACAAC ATCTTGTGGG CGCGCATGCT GAGCGGCTAC ACCGAGTTTC CGGACGAGGA GCGGGCGAAG CTGCCGCCCG CGCAGCACAA GCTCGTCAGG CAGCATCCCG TGTCGGGACG CAAGACGCTC TATCTGTCGG GGCACGCGAC GCACGTAGTG GACTGGCCGA TTCCCGAAGG CCGCGACCTG TTGCGCGAAT TGATGGAATT CGCGACGCAG CCCCAGTTCG TCTATACCCA TAAATGGCAC GAACGCGATC TGGTGATGTG GGACAACCGC GCGTTGATGC ACCGCGGGCG CCGCCATATT CCCGATACTG ACCCGCGCGA GATGCATCGC GCGACGGTAA TGGACGATCC TGCGTGGACG CGAGCGGACA GCAGCGTGCA CAGCGAAGCG GGAACGTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T